Effects of Genetic Variation in the 20th Intron of Sansui Duck ATP2A2 Gene on Eggshell Quality

In order to explore the influence of the polymorphism in the 20intron region of the Sansui duck ATP2A2 gene on the eggshell quality,this study used Primer Premier 5 software to design and synthesize a pair of primers in the 20 intron region,two-way direct sequencing and sequence alignment to mine SNPs Sites,SPSS 18.0 software was used to analyze the relationship between SNP sites and eggshell quality of Sansui duck.Three SNP sites were found in the 20 intron region of the ATP2A2 gene:g.40874 T>C,g.40920 G>A and g.40990 T=C,all of which were moderately polymorphic,at the site g.40874 T>C and g.40920 G>A both deviated significantly from Hardy-Weinberg equilibrium(P>0.05),position g.40990 T=C accords with Hardy-Weinberg equilibrium(P<0.05),and position g.40874 T>C There is a strong linkage disequilibrium between g.40990 T=C;a total of 4 haplotypes and 9 double types were detected at 3 SNP loci;the results of association analysis showed that g.40874 T>C mutation had a significant effect on eggshell strength and eggshell weight.The eggshell strength of CC genotype was significantly higher than that of TC and TT genotypes(P<0.05),the eggshell weight of CC genotype was significantly higher than that of TC genotype(P<0.05),and 40990 T=C mutation had a significant effect on eggshell strength.The eggshell strength of TC genotype was significantly higher than that of the TT genotype(P<0.05).In summary,the g.40874 T>C and g.40990 T=C found in the 20th intron region of the Sansui duck ATP2A2 gene may be the marker sites that affect the quality of the eggshell.

Run-and-Tumble Dynamics and Mechanotaxis Discovered in Microglial Migration

Microglia are resident macrophage cells in the central nervous system that search for pathogens or abnormal neural activities and migrate to resolve the issues.The effective search and targeted motion of macrophages mean dearly to maintaining a healthy brain,yet little is known about their migration dynamics.In this work,we study microglial motion with and without the presence of external mechanostimuli.We discover that the cells are promptly attracted by the applied forces(i.e.,mechanotaxis),which is a tactic behavior as yet unconfirmed in microglia.Meanwhile,in both the explorative and the targeted migration,microglia display dynamics that is strikingly analogous to bacterial run-and-tumble motion.A closer examination reveals that microglial run-and-tumble is more sophisticated,e.g.,they display a short-term memory when tumbling and rely on active steering during runs to achieve mechanotaxis,probably via the responses of mechanosensitive ion channels.These differences reflect the sharp contrast between microglia and bacteria cells(eukaryotes vs.prokaryotes)and their environments(compact tissue vs.fluid).Further analyses suggest that the reported migration dynamics has an optimal search efficiency and is shared among a subset of immune cells(human monocyte and macrophage).This work reveals a fruitful analogy between the locomotion of 2 remote systems and provides a framework for studying immune cells exploring complex environments.

ITPR1基因过表达对鸭子宫上皮细胞Ca2+浓度、脂质含量及钙转运相关基因的调控效应

肌醇1,4,5-三磷酸受体Ⅰ型(Inositol1,4,5-trisphosphatereceptor1,ITPR1)是细胞内Ca2+释放的重要通道。为探讨ITPR1基因过表达对鸭子宫上皮细胞Ca2+浓度和脂质含量的调控效应及其对钙转运相关基因的影响,文中构建鸭ITPR1基因结构域的真核表达载体,转染鸭子宫上皮细胞,检测细胞ITPR1基因的过表达量、Ca2+浓度、细胞的脂质含量以及其他6个钙转运相关基因的表达量。结果显示,转染后子宫上皮细胞内的Ca2+浓度显著降低(P<0.05),甘油三酯含量极显著升高(P<0.01),高密度脂蛋白含量极显著降低(P<0.01);相关性分析结果显示,C端ITPR1基因过表达量与总胆固醇含量呈极显著正相关(P<0.01),与低密度脂蛋白含量呈显著正相关(P<0.05), N端ITPR1基因过表达量与甘油三酯含量呈极显著正相关(P<0.01),与Ca2+浓度呈显著负相关(P<0.05);RT-qPCR结果显示,C端ITPR1基因过表达对IP3R2、VDAC2、CAV1基因的表达有显著抑制作用,N端ITPR1基因过表达对IP3R3、CACNA2D1基因的表达有显著促进作用。总之,ITPR1基因过表达能促进鸭子宫上皮细胞内Ca2+释放,促进甘油三酯、低密度脂蛋白和胆固醇的合成,抑制高密度脂蛋白的生成,且ITPR1基因过表达对6个钙转运相关基因的表达均产生影响。

CircRNA.0007127 triggers apoptosis through the miR-513a-5p/CASP8 axis in K-562 cells

Background:Circular RNAs(circR NAs)are covalently closed single-stranded RNAs with multiple biological functions.CircRNA.0007127 is derived from the carbon catabolite repression 4-negative on TATA-less(CCR4-NOT)complex subunit2(CNOT2),which was found to regulate tumor cell apoptosis through caspase pathway.Methods:Potential circR NA.0007127 target microRNAs(miRNAs)were analyzed by miRanda,TargetScan,and RNAhybrid software,and the miRNAs with binding sites for apoptosis-related genes were screened.The roles of circR NA.0007127 and its downstream target,microR NA(miR)-513a-5p,were validated by quantitative real-time polymerase chain reaction(qPCR),flow cytometry,mitochondrial membrane potential,immunofluorescence,western blot,and caspase-8(CASP8)protein activity in vitro in HO-induced K-562 cells.The circRNA.0007127-miR-513a-5p and CASP8-miR-513a-5p interactions were verified by luciferase reporter assays.Results:Silencing circRNA.0007127 decreased cell apoptosis by inhibiting CASP8 pathway activation in K-562 cells.Compared with the control group,the expression of CASP8 was reduced by 50%and the 43-kD fragment of CASP8 protein was significantly reduced(P≤0.05).The luciferase reporting assay showed that circRNA.0007127 combined with miR-513a-5p or CASP8,with extremely significant differences(P≤0.001).The overexpression of miR-513a-5p inhibited the gene expression level of CASP8in a human myeloid leukemia cell model(75%change)and the level of a 43-kD fragment of CASP8 protein(P small-interfering RNA(siRNA)and the miR-≤0.01).The rescue experiment showed that cotransfection with circRNA.0007127513a-5p inhibitor increased CASP8 gene expression and the apoptosis rate,suggesting that the miR-513a-5p inhibitor is a circRNA.0007127siRNA antagonist.Conclusions:CircRNA.0007127 regulates K-562 cell apoptosis through the miR-513a-5p/CASP8 axis,which can serve as a novel powerful molecular target for K-562 cells.

JUNO sensitivity on proton decay p→νK^(+)searches

The Jiangmen Underground Neutrino Observatory(JUNO)is a large liquid scintillator detector designed to explore many topics in fundamental physics.In this study,the potential of searching for proton decay in the p→νK^(+)mode with JUNO is investigated.The kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for identification.Moreover,the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed signals.Based on these advantages,the detection efficiency for the proton decay via p→νK^(+)is 36.9%±4.9%with a background level of 0.2±0.05(syst)±0.2(stat)events after 10 years of data collection.The estimated sensitivity based on 200 kton-years of exposure is 9.6×1033 years,which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies.

Feasibility and physics potential of detecting ^(8)B solar neutrinos at JUNO

The Jiangmen Underground Neutrino Observatory(JUNO)features a 20 kt multi-purpose underground liquid scintillator sphere as its main detector.Some of JUNO's features make it an excellent location for^8B solar neutrino measurements,such as its low-energy threshold,high energy resolution compared with water Cherenkov detectors,and much larger target mass compared with previous liquid scintillator detectors.In this paper,we present a comprehensive assessment of JUNO's potential for detecting^8B solar neutrinos via the neutrino-electron elastic scattering process.A reduced 2 MeV threshold for the recoil electron energy is found to be achievable,assuming that the intrinsic radioactive background^(238)U and^(232)Th in the liquid scintillator can be controlled to 10^(-17)g/g.With ten years of data acquisition,approximately 60,000 signal and 30,000 background events are expected.This large sample will enable an examination of the distortion of the recoil electron spectrum that is dominated by the neutrino flavor transformation in the dense solar matter,which will shed new light on the inconsistency between the measured electron spectra and the predictions of the standard three-flavor neutrino oscillation framework.IfDelta m^(2)_(21)=4.8times10^(-5);(7.5times10^(-5))eV^(2),JUNO can provide evidence of neutrino oscillation in the Earth at approximately the 3sigma(2sigma)level by measuring the non-zero signal rate variation with respect to the solar zenith angle.Moreover,JUNO can simultaneously measureDelta m^2_(21)using^8B solar neutrinos to a precision of 20% or better,depending on the central value,and to sub-percent precision using reactor antineutrinos.A comparison of these two measurements from the same detector will help understand the current mild inconsistency between the value of Delta m^2_(21)reported by solar neutrino experiments and the KamLAND experiment.

“议程设置”视域下党的七大相关文献出版研究

中国共产党第七次全国代表大会是中国共产党以及中国历史上一座不朽的里程碑。文献研究发现,1938年至1948年,党领导下的出版工作者深入群众,用创造性的工作不断赢得公信力,以“议程设置”的方式完成了关于七大的一系列文献出版活动,出版了大量历史性文献,对七大成功召开和宣传七大精神产生了至关重要的作用,在党领导下的出版事业中具有典型性的价值和意义。

Sub-percent precision measurement of neutrino oscillation parameters with JUNO

JUNO is a multi-purpose neutrino observatory under construction in the south of China.This publication presents new sensitivity estimates for the measurement of the △m_(31)^(2),△m_(21)^(2),sin^(2)θ_(12),and sin^(2)θ_(13) oscillation parameters using reactor antineutrinos,which is one of the primary physics goals of the experiment.The sensitivities are obtained using the best knowledge available to date on the location and overburden of the experimental site,the nuclear reactors in the surrounding area and beyond,the detector response uncertainties,and the reactor antineutrino spectral shape constraints expected from the TAO satellite detector.It is found that the △m_(21)^(2) and sin^(2)θ_(12) oscillation parameters will be determined to 0.5%precision or better in six years of data collection.In the same period,the △m_(31)^(2) parameter will be determined to about 0.2%precision for each mass ordering hypothesis.The new precision represents approximately an order of magnitude improvement over existing constraints for these three parameters.