<div style="text-align:justify;"> Pomegranate flowers as row materials were used for extraction of polysaccharides by water-extraction and alcohol-precipitation method. After purification, the physical...<div style="text-align:justify;"> Pomegranate flowers as row materials were used for extraction of polysaccharides by water-extraction and alcohol-precipitation method. After purification, the physical and chemical properties, structure, monosaccharide composition and molecular weight were studied. The results showed that the polysaccharides from pomegranate flowers mainly contained two kinds of water soluble acidic polysaccharides, and monosaccharide composition were arabinose and galactose, both contained hydroxyl, carboxyl, amino, hydroxyl radical, sulfate, beta glycosidic bond and alpha glycosidic bond structure. The molecular weight of PP1 and PP2 were 6.16 × 104 (±6.6%) and 9.01 × 104 (±3.2%), respectively. The results of this study laid the foundation for further development and application of polysaccharides from pomegranate flowers. </div>展开更多
Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China.The B.syzigachne(AH-02)population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl.To investigate the function of GT7...Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China.The B.syzigachne(AH-02)population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl.To investigate the function of GT73C1 in this population,the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction,and the sequence was 100%consistent with the transcriptome data.Its phylogenetic tree was displayed and annotated using FigTree v1.4.4.The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants.Furthermore,the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl,which was consistent with the findings from the whole plant bioassay.These results indicate that GT73C1 is closely related to the metabolic resistance of B.distachyon.展开更多
文摘<div style="text-align:justify;"> Pomegranate flowers as row materials were used for extraction of polysaccharides by water-extraction and alcohol-precipitation method. After purification, the physical and chemical properties, structure, monosaccharide composition and molecular weight were studied. The results showed that the polysaccharides from pomegranate flowers mainly contained two kinds of water soluble acidic polysaccharides, and monosaccharide composition were arabinose and galactose, both contained hydroxyl, carboxyl, amino, hydroxyl radical, sulfate, beta glycosidic bond and alpha glycosidic bond structure. The molecular weight of PP1 and PP2 were 6.16 × 104 (±6.6%) and 9.01 × 104 (±3.2%), respectively. The results of this study laid the foundation for further development and application of polysaccharides from pomegranate flowers. </div>
基金Scientific Research Foundation for Ph.D.Programs of Zaozhuang University(21/1020708)Science and Technology Program of Zaozhuang(2020NS20)+1 种基金Project of Shandong Province Higher Educational Science and Technology Program(J18KA134)Shandong Provincial Natural Science Foundation(ZR2019PC011).
文摘Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China.The B.syzigachne(AH-02)population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl.To investigate the function of GT73C1 in this population,the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction,and the sequence was 100%consistent with the transcriptome data.Its phylogenetic tree was displayed and annotated using FigTree v1.4.4.The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants.Furthermore,the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl,which was consistent with the findings from the whole plant bioassay.These results indicate that GT73C1 is closely related to the metabolic resistance of B.distachyon.