Accumulating studies reveal that mesenchymal stem cells(MSCs)promote skin wound healing mainly through the paracrine effects.Exosomes,one of the crucial paracrine mediators in wound healing,are cell-derived nanosized ...Accumulating studies reveal that mesenchymal stem cells(MSCs)promote skin wound healing mainly through the paracrine effects.Exosomes,one of the crucial paracrine mediators in wound healing,are cell-derived nanosized membranous vesicles containing diverse bioactive cargoes.With the potent ability of modulating skin cell behaviors,MSC-derived exosomes(MSC-Exos)are regarded as a promising nanomaterial for regenerative wound therapy.Under hostile conditions,MSC-Exos are efficient in protecting skin cells from severe damage and restoring their function.According to recent studies,MSC-Exos possess remarkable pro-healing effects in a variety of skin wounds,typically resulting in increased wound closure,inhibited scar tissue formation,and better restoration of skin function.To further enhance the therapeutic potential of MSC-Exos,the development of applicable pretreatment strategies and the optimization of exosome delivery are under intensive investigation.Herein,we summarize current research progress of MSC-Exos for skin wound treatment,with an emphasis on the biological effects of these nanovesicles,the repair mechanisms,and future challenges in clinical translation.展开更多
Background Robot-assisted minimally invasive esophagectomy(RAMIE)is becoming increasingly popular as a treatment for esophageal cancer.The purpose of this study was to use the da Vinci robotic system(Intuitive Surgica...Background Robot-assisted minimally invasive esophagectomy(RAMIE)is becoming increasingly popular as a treatment for esophageal cancer.The purpose of this study was to use the da Vinci robotic system(Intuitive Surgical,Sunnyvale,CA,USA)to perform 111 consecutive cases of radical esophageal cancer and report the perioperative data of surgery and postoperative complications and short-term oncological outcomes.Methods We retrospectively analyzed 111 patients who underwent RAMIE conducted at Fujian Medical University Union Hospital from August 2016 to January 2021.Each patient’s characteristics,clinicopathological stage,postoperative pathological stage,surgery outcome,postoperative recovery,and short-term oncological outcomes were analyzed.Results Of the 111 patients who underwent RAMIE,77 were male and 34 were female,and the mean age was 62.1±8.8 years.Twenty-seven patients(24.3%)received preoperative neoadjuvant therapies,the most popular of which was preoperative induction chemotherapy in 16 cases(14.4%),followed by preoperative induction radiotherapy in 8 cases(7.2%)and preoperative induction chemotherapy plus immunization in 3 cases(2.7%).The vast majority of patients(110/111,99.1%)underwent radical resection,with a mean intraoperative bleeding amount of 99.9±68.4 mL and a mean operative time of 271.9±70.0 min.The mean total number of lymph nodes removed was 40.9±16.7,including 21.8±9.0 thoracic lymph nodes.Fifty-five(49.6%)patients had lymph node metastases,including 17(15.3%)with lymph node metastases in the left recurrent laryngeal nerve,24(21.6%)with lymph node metastases in the right recurrent laryngeal nerve,and 7(6.3%)with lymph node metastases in the bilateral recurrent laryngeal nerve.The positive rate of left recurrent nerve lymph nodes was 4.77%,and he positive rate of right recurrent nerve lymph nodes was 8.38%.The main postoperative complications included pulmonary infection in 24 cases(21.6%),celiac disease in 3 cases(2.7%),tracheoesophageal fistula in 1 case(0.9%),anastomotic fistula in 3 cases(2.7%),postoperative cardiac arrhythmias in 11 cases(9.9%),VTE in 3 cases(2.7%),and pleural effusion(requiring postoperative tube drainage)in 13 cases(11.7%).Additionally,2 cases of postoperative pneumothorax(1.8%),1 case of poor incision healing(0.9%),1 case of incomplete bowel obstruction(0.9%),1 case of neck hematoma(0.9%),and 1 case of postoperative admission to the intensive care unit(0.9%)occurred.The median length of stay was 10.9±6.1 days,and there were no cases of perioperative death.Conclusions Robotic esophageal cancer radical surgery is safe and feasible.The surgeon can be proficient in thoracic robotic surgery and mediastinal lymph node dissection and achieve high levels of perioperative safety and short-term efficacy.展开更多
As an emerging type of adult stem cell featuring non-invasive acquisition,urine-derived stem cells(USCs)have shown great potential for applications in tissue engineering and regenerative medicine.With a growing amount...As an emerging type of adult stem cell featuring non-invasive acquisition,urine-derived stem cells(USCs)have shown great potential for applications in tissue engineering and regenerative medicine.With a growing amount of research on the topic,the effectiveness of USCs in various disease models has been shown and the underlying mechanisms have also been explored,though many aspects still remain unclear.In this review,we aim to provide an up-to-date overview of the biological characteristics of USCs and their applications in skin,bone and articular cartilage repair.In addition to the identification procedure of USCs,we also summarize current knowledge of the underlying repair mechanisms and application modes of USCs.Potential concerns and perspectives have also been summarized.展开更多
基金supported by the National Natural Science Foundation of China(Nos.32071331 and 31600792)Post-Doctor Research Project,West China Hospital,Sichuan University(No.2018HXBH053).
文摘Accumulating studies reveal that mesenchymal stem cells(MSCs)promote skin wound healing mainly through the paracrine effects.Exosomes,one of the crucial paracrine mediators in wound healing,are cell-derived nanosized membranous vesicles containing diverse bioactive cargoes.With the potent ability of modulating skin cell behaviors,MSC-derived exosomes(MSC-Exos)are regarded as a promising nanomaterial for regenerative wound therapy.Under hostile conditions,MSC-Exos are efficient in protecting skin cells from severe damage and restoring their function.According to recent studies,MSC-Exos possess remarkable pro-healing effects in a variety of skin wounds,typically resulting in increased wound closure,inhibited scar tissue formation,and better restoration of skin function.To further enhance the therapeutic potential of MSC-Exos,the development of applicable pretreatment strategies and the optimization of exosome delivery are under intensive investigation.Herein,we summarize current research progress of MSC-Exos for skin wound treatment,with an emphasis on the biological effects of these nanovesicles,the repair mechanisms,and future challenges in clinical translation.
基金supported by the National key clinical specialty of Thoracic Surgery.
文摘Background Robot-assisted minimally invasive esophagectomy(RAMIE)is becoming increasingly popular as a treatment for esophageal cancer.The purpose of this study was to use the da Vinci robotic system(Intuitive Surgical,Sunnyvale,CA,USA)to perform 111 consecutive cases of radical esophageal cancer and report the perioperative data of surgery and postoperative complications and short-term oncological outcomes.Methods We retrospectively analyzed 111 patients who underwent RAMIE conducted at Fujian Medical University Union Hospital from August 2016 to January 2021.Each patient’s characteristics,clinicopathological stage,postoperative pathological stage,surgery outcome,postoperative recovery,and short-term oncological outcomes were analyzed.Results Of the 111 patients who underwent RAMIE,77 were male and 34 were female,and the mean age was 62.1±8.8 years.Twenty-seven patients(24.3%)received preoperative neoadjuvant therapies,the most popular of which was preoperative induction chemotherapy in 16 cases(14.4%),followed by preoperative induction radiotherapy in 8 cases(7.2%)and preoperative induction chemotherapy plus immunization in 3 cases(2.7%).The vast majority of patients(110/111,99.1%)underwent radical resection,with a mean intraoperative bleeding amount of 99.9±68.4 mL and a mean operative time of 271.9±70.0 min.The mean total number of lymph nodes removed was 40.9±16.7,including 21.8±9.0 thoracic lymph nodes.Fifty-five(49.6%)patients had lymph node metastases,including 17(15.3%)with lymph node metastases in the left recurrent laryngeal nerve,24(21.6%)with lymph node metastases in the right recurrent laryngeal nerve,and 7(6.3%)with lymph node metastases in the bilateral recurrent laryngeal nerve.The positive rate of left recurrent nerve lymph nodes was 4.77%,and he positive rate of right recurrent nerve lymph nodes was 8.38%.The main postoperative complications included pulmonary infection in 24 cases(21.6%),celiac disease in 3 cases(2.7%),tracheoesophageal fistula in 1 case(0.9%),anastomotic fistula in 3 cases(2.7%),postoperative cardiac arrhythmias in 11 cases(9.9%),VTE in 3 cases(2.7%),and pleural effusion(requiring postoperative tube drainage)in 13 cases(11.7%).Additionally,2 cases of postoperative pneumothorax(1.8%),1 case of poor incision healing(0.9%),1 case of incomplete bowel obstruction(0.9%),1 case of neck hematoma(0.9%),and 1 case of postoperative admission to the intensive care unit(0.9%)occurred.The median length of stay was 10.9±6.1 days,and there were no cases of perioperative death.Conclusions Robotic esophageal cancer radical surgery is safe and feasible.The surgeon can be proficient in thoracic robotic surgery and mediastinal lymph node dissection and achieve high levels of perioperative safety and short-term efficacy.
基金supported by National Natural Science Foundation of China(Grant No.31771065)the 1.3.5 Project for Disciplines of Excellence,West China Hospital,Sichuan University(Grant No.ZYJC18002).
文摘As an emerging type of adult stem cell featuring non-invasive acquisition,urine-derived stem cells(USCs)have shown great potential for applications in tissue engineering and regenerative medicine.With a growing amount of research on the topic,the effectiveness of USCs in various disease models has been shown and the underlying mechanisms have also been explored,though many aspects still remain unclear.In this review,we aim to provide an up-to-date overview of the biological characteristics of USCs and their applications in skin,bone and articular cartilage repair.In addition to the identification procedure of USCs,we also summarize current knowledge of the underlying repair mechanisms and application modes of USCs.Potential concerns and perspectives have also been summarized.
