Nonylphenol ethoxylate (NPEO)-degrading bacteria were isolated from activated sludge using an improved isolation method, and the corresponding degradation behaviours were investigated. Eight NPEO-degrading strains dis...Nonylphenol ethoxylate (NPEO)-degrading bacteria were isolated from activated sludge using an improved isolation method, and the corresponding degradation behaviours were investigated. Eight NPEO-degrading strains distributed in genera Pseudomonas, Sphingomonas, Sphingobium, Cupriavidus, Ralstonia, Achromobacter, and Staphylococcus were acquired. The latter five genera have never been reported for the degradation of NPEOs. Four degradation patterns were observed for the eight pure strains. In pattern A, NPE...展开更多
In recounting Dr.James M.Tiedje's outstanding research achievements spanning the past 55 years,it is easy to overlook his early and mid-career endeavors.Specifically,his contribution to the aerobic degradation of ...In recounting Dr.James M.Tiedje's outstanding research achievements spanning the past 55 years,it is easy to overlook his early and mid-career endeavors.Specifically,his contribution to the aerobic degradation of pesticides and other chemicals,as well as methanogenic degradation of those compounds retains their brilliance.Many researchers in environmental microbiology have gained invaluable knowledge from these studies,which have been applied to the elucidation of previously uncultivated microorganisms.展开更多
Archaea,one of the three domains of life along with Bacteria and Eukarya,contains ancient life forms such as methanogen that are observed today on Earth,and one lineage Asgard archaea is also considered the closest an...Archaea,one of the three domains of life along with Bacteria and Eukarya,contains ancient life forms such as methanogen that are observed today on Earth,and one lineage Asgard archaea is also considered the closest ancestor of Eukarya.Recently,with the development of interdisciplinary studies from Earth and Life sciences,archaeal organisms are considered to play pivotal roles in geochemical cycling in nature.However,our understanding of the attributes,origin and evolution,geochemical and ecological functions of Archaea is hampered by the scarcity of archaeal isolates,which has represented a challenge to researchers for the last 40 years.Cultivation-independent sequencing and phylogenomic analyses have demonstrated a considerable diversity of Archaea with more than 20 novel phyla.However,only four archaeal phyla have cultured representatives,leaving large gaps in our knowledge of the metabolic capabilities and ecological functions of the majority of archaeal strains identified exclusively by DNA sequencing.In this review,we summarize the discovery and development of archaeal research,highlight the knowledge gap between uncultured and cultured archaeal microbes,and call on the importance of devoting greater research efforts to archaeal cultivation.Finally,we outlined new ideas and strategic approaches,namely,(1)genome-based methods,(2)microbial network information-based methods,(3)genome-scale model-guided methods,and(4)machine learning methods,to enable the cultivation of uncultivated archaeal microbes using accumulated high-throughput sequencing data.展开更多
基金the National Natural Sci-ence Foundation of China (No. 50578153, 50525824)the Ministry of Science and Technology, China (No.2006DFA91870)
文摘Nonylphenol ethoxylate (NPEO)-degrading bacteria were isolated from activated sludge using an improved isolation method, and the corresponding degradation behaviours were investigated. Eight NPEO-degrading strains distributed in genera Pseudomonas, Sphingomonas, Sphingobium, Cupriavidus, Ralstonia, Achromobacter, and Staphylococcus were acquired. The latter five genera have never been reported for the degradation of NPEOs. Four degradation patterns were observed for the eight pure strains. In pattern A, NPE...
文摘In recounting Dr.James M.Tiedje's outstanding research achievements spanning the past 55 years,it is easy to overlook his early and mid-career endeavors.Specifically,his contribution to the aerobic degradation of pesticides and other chemicals,as well as methanogenic degradation of those compounds retains their brilliance.Many researchers in environmental microbiology have gained invaluable knowledge from these studies,which have been applied to the elucidation of previously uncultivated microorganisms.
基金国家重点研发项目(批准号:2016YFA0601102)中国大洋矿产开发协会(编号:DY135-B2-12)+6 种基金COMRA项目(编号:DY135-B2-12)国家自然科学基金项目(批准号:41525011、41921006、41902313、91751205、92051116)上海市自然科学基金项目(编号:20ZR1428000)上海交通大学交叉学科项目(编号:20CX-01)日本学术振兴会(KAKENHI Grant No.18H05295)中国科学院“科学”号高端用户项目(编号:KEXUE2019GZ06)深部生命国际研究中心(IC-DLI)资助。
基金Funding for this research was provided by the National Natural Science Foundation of China (Grant No. 20921140094) and the the Knowledge Innovation Project of Chinese Academy of Sciences (KSCX2-YW-G-054).
基金supported by the State Key R&D Project of China(Grant No.2016YFA0601102)the China Ocean Mineral Resources R&D Association(Grant No.DY135-B2-12)+6 种基金COMRA Project DY135-B2-12the National Nature Science Foundation of China(Grant Nos.41525011,41921006,41902313,91751205&92051116)the Natural Science Foundation of Shanghai(Grant No.20ZR1428000)the Shanghai Jiao Tong University Interdisciplinary Grant(Grant No.20CX-01)Japan Society for the Promotion of Science(JSPS)(KAKENHI Grant 18H05295)This is also a contribution to the Center for Ocean Mega-Science,Chinese Academy of Sciences,the Senior User Project of RV KEXUE(Grant No.KEXUE2019GZ06)the International Center for Deep-life Investigation(IC-DLI).
文摘Archaea,one of the three domains of life along with Bacteria and Eukarya,contains ancient life forms such as methanogen that are observed today on Earth,and one lineage Asgard archaea is also considered the closest ancestor of Eukarya.Recently,with the development of interdisciplinary studies from Earth and Life sciences,archaeal organisms are considered to play pivotal roles in geochemical cycling in nature.However,our understanding of the attributes,origin and evolution,geochemical and ecological functions of Archaea is hampered by the scarcity of archaeal isolates,which has represented a challenge to researchers for the last 40 years.Cultivation-independent sequencing and phylogenomic analyses have demonstrated a considerable diversity of Archaea with more than 20 novel phyla.However,only four archaeal phyla have cultured representatives,leaving large gaps in our knowledge of the metabolic capabilities and ecological functions of the majority of archaeal strains identified exclusively by DNA sequencing.In this review,we summarize the discovery and development of archaeal research,highlight the knowledge gap between uncultured and cultured archaeal microbes,and call on the importance of devoting greater research efforts to archaeal cultivation.Finally,we outlined new ideas and strategic approaches,namely,(1)genome-based methods,(2)microbial network information-based methods,(3)genome-scale model-guided methods,and(4)machine learning methods,to enable the cultivation of uncultivated archaeal microbes using accumulated high-throughput sequencing data.