A method for producing size- and shape-con-trolled calcium alginate beads with immobilized proteins was developed. Unlike previous cal-cium alginate bead production methods, pro-tein-immobilized alginate beads with un...A method for producing size- and shape-con-trolled calcium alginate beads with immobilized proteins was developed. Unlike previous cal-cium alginate bead production methods, pro-tein-immobilized alginate beads with uniform shape and sizes less then 20 micrometers in diameter could successfully be produced by using sonic vibration. BSA and FITC-conjugated anti-BSA antibodies were used to confirm pro-tein immobilization in the alginate beads. Pro-tein diffusion from the beads could be reduced to less than 10% by cross-linking the proteins to the alginate with 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxysul-fosuccinimide (NHSS). The calcium alginate beads could also be arranged freely on a slide glass by using a femtosecond laser.展开更多
Background: Commonly, skin and clothing surface contamination screening (radiation survey) is performed for residents of an area to confirm the levels of residual contamination after nuclear power disasters. Examiners...Background: Commonly, skin and clothing surface contamination screening (radiation survey) is performed for residents of an area to confirm the levels of residual contamination after nuclear power disasters. Examiners for such a screening often wear white radiation hazmat suits. However, it has been reported that examinees tend to feel tension or anxiety. In this study, we focus on the color of the radiation hazmat suit as one measure to reduce residents’ tension or anxiety. This study aims to determine the common conceptions of radiation hazmat suits based on color. Methods: An anonymous self-administered questionnaire survey was conducted with a total of 262 participants consisting of nursing students, administrative staff of a university, general citizens, and mothers raising their children in the Tohoku region in Japan. The common impressions of radiation hazmat suits, which are typically white, were measured using the Multiple Affective States Scale. This scale can measure the affective state brought about by clothing. The color of white (W) was defined as a standard color commonly used for hazmat suits;in order to determine the common impressions of radiation hazmat suits based on color, the following additional colors were shown to the participants: salmon pink (P), ivory (I), and light green (G). Results: There were no sex differences regarding common impressions of radiation hazmat suits. For white radiation hazmat suits, a high score reflecting the characteristic of “capable” was obtained, while the scores for “cheerful” and “calm” were low, and those for “depressed” and “tense” were high. The colors that showed the highest score for “calm” as a positive affirmative state were I and P. W and P reflected a high “ashamed” score, and the “tense” score for G was higher for the participants in their 20s than for those in their 30s and older. Conclusions: Results show that the color of hazmat suits provokes far greater feelings of anxiety and tension on participants than previously thought. Radiation hazmat suits may be further improved based on the results of this study.展开更多
文摘A method for producing size- and shape-con-trolled calcium alginate beads with immobilized proteins was developed. Unlike previous cal-cium alginate bead production methods, pro-tein-immobilized alginate beads with uniform shape and sizes less then 20 micrometers in diameter could successfully be produced by using sonic vibration. BSA and FITC-conjugated anti-BSA antibodies were used to confirm pro-tein immobilization in the alginate beads. Pro-tein diffusion from the beads could be reduced to less than 10% by cross-linking the proteins to the alginate with 1-ethyl-3-(3-dimethylamino-propyl)carbodiimide (EDC) and N-hydroxysul-fosuccinimide (NHSS). The calcium alginate beads could also be arranged freely on a slide glass by using a femtosecond laser.
文摘Background: Commonly, skin and clothing surface contamination screening (radiation survey) is performed for residents of an area to confirm the levels of residual contamination after nuclear power disasters. Examiners for such a screening often wear white radiation hazmat suits. However, it has been reported that examinees tend to feel tension or anxiety. In this study, we focus on the color of the radiation hazmat suit as one measure to reduce residents’ tension or anxiety. This study aims to determine the common conceptions of radiation hazmat suits based on color. Methods: An anonymous self-administered questionnaire survey was conducted with a total of 262 participants consisting of nursing students, administrative staff of a university, general citizens, and mothers raising their children in the Tohoku region in Japan. The common impressions of radiation hazmat suits, which are typically white, were measured using the Multiple Affective States Scale. This scale can measure the affective state brought about by clothing. The color of white (W) was defined as a standard color commonly used for hazmat suits;in order to determine the common impressions of radiation hazmat suits based on color, the following additional colors were shown to the participants: salmon pink (P), ivory (I), and light green (G). Results: There were no sex differences regarding common impressions of radiation hazmat suits. For white radiation hazmat suits, a high score reflecting the characteristic of “capable” was obtained, while the scores for “cheerful” and “calm” were low, and those for “depressed” and “tense” were high. The colors that showed the highest score for “calm” as a positive affirmative state were I and P. W and P reflected a high “ashamed” score, and the “tense” score for G was higher for the participants in their 20s than for those in their 30s and older. Conclusions: Results show that the color of hazmat suits provokes far greater feelings of anxiety and tension on participants than previously thought. Radiation hazmat suits may be further improved based on the results of this study.
