Reducing the global cancer burden with gastrointestinal screening: China's 30 years practice

Gastrointestinal(GI) cancers include esophageal [EC(International Classification of Disease 10^(th) revision: C15)], gastric [GC(C16)], and colorectal [CRC(C18-C21)] cancers. China is a high-incidence country of GI cancers.

Practical Experience of Management of Urban Parks at Home and Abroad

By systematically reviewing the practical experience of management of urban parks at home and abroad,the advanced experience in organization,management system,operation mechanism and social participation were summarized,and the optimization and improvement measures for the construction and management of urban parks in Chengdu were proposed to provide reference for the high-level construction,high-quality experience and efficient management of urban parks in the construction of park city demonstration zone in Chengdu.

Optimization on microstructure,mechanical properties and damping capacities of duplex structured Mg–8Li–4Zn–1Mn alloys

Optimizing the mechanical properties and damping capacity of the duplex-structured Mg–Li–Zn–Mn alloy by tailoring the microstructure via hot extrusion was investigated.The results show that the Mg–8Li–4Zn–1Mn alloy is mainly composed ofα-Mg,β-Li,Mg–Li–Zn and Mn phases.The microstructure of the test alloy is refined owing to dynamic recrystallization(DRX)during hot extrusion.After hot extrusion,the crushed precipitates are uniformly distributed in the test alloy.The yield strength(YS),ultimate tensile strength(UTS),and elongation(EL)of as-extruded alloy reach 156 MPa,208 MPa,and 32.3%,respectively,which are much better than that of as-cast alloy.Furthermore,the as-extruded and as-cast alloys both exhibit superior damping capacities,with the damping capacity(Q^(-1))of 0.030 and 0.033 at the strain amplitude of 2×10^(-3),respectively.The mechanical properties of the test alloy can be significantly improved by hot extrusion,whereas the damping capacities have no noticeable change,which indicates that the duplex-structured Mg–Li alloys with appropriate mechanical properties and damping properties can be obtained by alloying and hot extrusion.

Metformin accelerates bone fracture healing by promoting type H vessel formation through inhibition of YAP1/TAZ expression

Due to increasing morbidity worldwide,fractures are becoming an emerging public health concern.This study aimed to investigate the effect of metformin on the healing of osteoporotic as well as normal fractures.Type H vessels have recently been identified as a bone-specific vascular subtype that supports osteogenesis.Here,we show that metformin accelerated fracture healing in both osteoporotic and normal mice.Moreover,metformin promoted angiogenesis in vitro under hypoxia as well as type H vessel formation throughout fracture healing.Mechanistically,metformin increased the expression of HIF-1α,an important positive regulator of type H vessel formation,by inhibiting the expression of YAP1/TAZ in calluses and hypoxia-cultured human microvascular endothelial cells(HMECs).The results of HIF-1αor YAP1/TAZ interference in hypoxia-cultured HMECs using si RNA further suggested that the enhancement of HIF-1αand its target genes by metformin is primarily through YAP1/TAZ inhibition.Finally,overexpression of YAP1/TAZ partially counteracted the effect of metformin in promoting type H vessel-induced angiogenesis-osteogenesis coupling during fracture repair.In summary,our findings suggest that metformin has the potential to be a therapeutic agent for fractures by promoting type H vessel formation through YAP1/TAZ inhibition.

Causal associations of brain structure with bone mineral density:a large-scale genetic correlation study

In this study,we aimed to investigate the causal associations of brain structure with bone mineral density(BMD).Based on the genome-wide association study(GWAS)summary statistics of 1325 brain imaging-derived phenotypes(BIDPs)of brain structure from the UK Biobank and GWAS summary datasets of 5 BMD locations,including the total body,femoral neck,lumbar spine,forearm,and heel from the GEFOS Consortium,linkage disequilibrium score regression(LDSC)was conducted to determine the genetic correlations,and Mendelian randomization(MR)was then performed to explore the causal relationship between the BIDPs and BMD.Several sensitivity analyses were performed to verify the strength and stability of the present MR outcomes.To increase confidence in our findings,we also performed confirmatory MR between BIDPs and osteoporosis.LDSC revealed that 1.93%of BIDPs,with a false discovery rate(FDR)<0.01,were genetically correlated with BMD.Additionally,we observed that 1.31%of BIDPs exhibited a significant causal relationship with BMD(FDR<0.01)through MR.Both the LDSC and MR results demonstrated that the BIDPs“Volume of normalized brain,”“Volume of gray matter in Left Inferior Frontal Gyrus,pars opercularis,”“Volume of Estimated Total Intra Cranial”and“Volume-ratio of brain segmentation/estimated total intracranial”had strong associations with BMD.Interestingly,our results showed that more left BIDPs were causally associated with BMD,especially within and around the left frontal region.In conclusion,a part of the brain structure causally influences BMD,which may provide important perspectives for the prevention of osteoporosis and offer valuable insights for further research on the brain-bone axis.

三叶青多糖增强Lewis肺癌小鼠抗肿瘤免疫反应

背景与目的肺癌具有高致病率和高致死率,但肺癌治疗仍缺乏低毒高效的抗肿瘤药物。三叶青多糖在抗肿瘤治疗方面具有开发价值。本文旨在观察三叶青多糖对Lewis肺癌小鼠免疫反应的影响,并探讨其分子机制。方法建立Lewis肺癌小鼠模型并采用随机数字表法进行分组。脾多肽组灌胃50 mg/kg脾多肽,三叶青多糖低、中、高剂量组分别灌胃62.5、125、250 mg/kg三叶青多糖,模型组和对照组灌胃等容积生理盐水。比较肿瘤形成、转移情况;苏木素-伊红(haematoxylin-eosin,HE)染色观察肿瘤细胞病理学变化;流式细胞术检测巨噬细胞吞噬能力、凋亡水平、M1/M2型极化水平及外周血T细胞亚群和细胞因子水平;噻唑蓝溴化四唑(methyl thiazolyldiphenyl tetrazolium,MTT)法检测巨噬细胞增殖活性;β-D-吡喃半乳糖苷氯酚红(chlorophenol red-β-D-galactopyranoside,CPRG)法检测树突状细胞(dendritic cell,DC)抗原提呈功能;实时荧光定量聚合酶链反应(real time quantitative polymerase chain reaction,RT-qPCR)和蛋白质免疫印迹法(Western blot,WB)检测肿瘤组织分化抗原簇47(cluster of differentiation 47,CD47)信使核糖核酸(messenger ribonucleic acid,mRNA)和蛋白表达及巨噬细胞中信号调节蛋白α(signal regulatory proteinα,SIRPα)表达。结果三叶青多糖3个剂量组和脾多肽组抑瘤率和抗转移率高于模型组,肿瘤组织病理损伤严重,巨噬细胞吞噬墨汁阳性率和吞噬肿瘤细胞效率升高,巨噬细胞凋亡率下降,巨噬细胞增殖活性、巨噬细胞向M1型极化比例、DC抗原提呈能力、CD4^(+)、CD4^(+)/CD8^(+)升高,血清肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白介素-1β(interleukin-1β,IL-1β)、肿瘤组织CD47、巨噬细胞蛋白酪氨酸磷酸酶1(SH2-containing protein tyrosine phosphatase 1,SHP-1)、蛋白酪氨酸磷酸酶2(SH2-containing protein tyrosine phosphatase 2,SHP-2)及磷酸化信号调节蛋白α(phosphorylation signal regulatory proteinα,p-SIRPα)表达下降,差异均有统计学意义(P<0.05)。上述指标三叶青多糖低剂量组与脾多肽组的差异均无统计学意义(P>0.05),且三叶青多糖的作用均呈剂量依赖性。结论三叶青多糖有抗Lewis肺癌小鼠肿瘤生长和转移及免疫反应的作用,其机制可能与抑制SIRP/CD47信号通路有关。

电解液中Cu(111)晶面电溶解/沉积势垒施加电荷相关性的跨尺度计算

电化学沉积和电化学腐蚀的核心问题是不同电压/电荷作用下的电极/电解质界面行为,其控制量是溶解/沉积反应路径的势垒,但是势垒的测量和计算难度比较大。本文采用密度泛函和连续介质耦合方法研究了不同加载电荷面密度下平整表面和含阶梯表面的Cu(111)面薄板电极直接和间接溶解/沉积两种路径的能量形态。结果发现,不同加载电荷面密度下溶质Cu原子在Cu(111)面的表面扩散和溶解过程中初末态能量分别和最高过渡态能量存在简单的线性关系,符合经典的Brønsted-Evans-Polanyi关系。在直接/间接溶解和沉积过程中,势垒和加载的电荷面密度呈线性或二次函数关系。通过这些表达式可以直接从稳态能量计算溶解/沉积和表面扩散的势垒,也可以直接计算不同加载电荷面密度下的势垒,极大的降低实验和计算工作量。通过拟合公式计算出不同临界加载电荷面密度时的势垒大小可以得出:对于溶解过程中,随着加载电荷面密度逐渐增大至0.135|e|/Å2,阶梯处原子首先以直接溶解的方式进入到电解质溶液中;对于沉积过程,随着加载电荷面密度降低至0.105|e|/Å2,电沉积首先发生在平整表面,并可越过较低的表面扩散势垒移动至台阶处,表面扩散是速率控制步骤。当加载电荷面密度进一步减小为0.086|e|/Å2,此时的沉积方式以直接沉积到阶梯位置为主。

经自然腔道内镜下胰腺包裹性坏死引流清创术

感染性胰腺坏死(IPN)是急性胰腺炎(AP)的严重局部并发症,其死亡率达32.0%。目前的共识认为对于怀疑或确诊的IPN,有创的干预治疗(包括经皮引流、内镜或手术)应尽可能推迟至发病4周后进行,使坏死组织液化并形成包裹性坏死(WON)。随着内镜技术发展,经自然腔道(胃或十二指肠)内镜下引流及清创术已被国内外指南推荐为WON的首选方法之一。该文详细阐述了WON的诊断与评估;内镜下引流与清创术的适应证、禁忌证、操作步骤、术后评估和并发症处理,最后介绍了近年内镜引流与清创治疗的研究进展。

分子前驱体衍生的氧化镍电极催化水氧化性能研究（英文）

太阳能分解水制氢是解决当前能源和环境危机的潜在手段之一.其中由于水氧化半反应涉及4个电子和4个质子的转移,因此是全分解水反应的瓶颈所在.为了发展高效的水氧化催化剂,降低水氧化过电位,人们付出了巨大的努力.目前活性最高的水氧化催化剂都是基于钌和铱的贵金属催化剂,高昂的成本阻碍了这些催化剂的规模化应用,因此人们尝试利用各种方法制备基于廉价金属的水氧化催化剂.2008年,Nocera课题组利用电沉积法从磷酸溶液中得到了高活性氧化钴催化剂,之后该法逐渐得到推广.最近,Spiccia和Allen课题组利用分子前驱体通过电沉积法制备了氧化镍催化剂,但其催化活性和稳定性有待进一步提高.本文将一个简单的镍配合物[Ni(en)_3]Cl_2(en=1,2-乙二胺)作为前驱体溶解到磷酸缓冲溶液中,在FTO基底上电沉积得到具有高催化活性的氧化镍水氧化催化剂.在pH=11的磷酸缓冲溶液中,由分子前驱体沉积所得到的NiO_x的催化电流达到1 mA/cm^2时的过电位为375 mV,且可稳定工作10 h以上.其催化过程中的Tafel斜率为46 mV/decade,表现出优异的动力学特性.该电极和之前文献中催化活性最高的从分子前驱体衍生得到的NiO_x相比展现出较大的优势.比如在1.3 V(相对于NHE)电压下,[Ni(en)3]Cl2衍生的NiO_x催化电流密度可以达到8.5 mA/cm^2,法拉第效率为98%.而Ni-氨基乙酸衍生的NiO_x在相同条件下催化电流密度为4 mA/cm^2,法拉第效率仅为60%.该工作充分证明以分子配合物作为前驱体是制备高效高稳定性多相水氧化催化剂的简便途径.有机配体和金属螯合的分子前驱体在结构上具有灵活可调的特性,从而有助于构建活性和效率更高的催化体系.

盐酸小檗碱在皮芯氨纶纤维中的负载与释放

采用皮芯湿法纺丝技术制备了包载盐酸小檗碱(BCH)为模型药物的氨纶纤维,分别通过扫描电镜、热重分析、差示量热扫描、单纱强力、白度、接触角测试表征和分析了纤维的形貌结构、药物分布、热/力学性能、白度及亲疏水性能。并针对氨纶纤维所包载的BCH进行了体外释放行为追踪。结果表明,皮芯湿法纺纤维成型良好,具有明显的皮芯结构,且BCH以无定形形式分布于纤维中。同时,研究发现,BCH的引入显著改善了纤维的热稳定性能,其热分解温度提高70℃以上,同时也增强了纤维表面的亲水性能,其中皮芯层均负载BCH的氨纶纤维表现为亲水性,接触角小于90°。此外,体外药物释放实验证实,在释放15 h时,仅芯层负载BCH的氨纶纤维(释放了～30%)相比皮芯层均负载BCH的氨纶纤维(释放了～50%)有较好的药物缓释效果。

Generation of male germ cells from induced pluripotent stem cells （iPS cells）： an in vitro and in vivo study

Recent studies have reported that induced pluripotent stem （iPS） cells from mice and humans can differentiate into primordial germ cells. However, whether iPS cells are capable of producing male germ cells is not known. The objective of this study was to investigate the differentiation potential of mouse iPS cells into spermatogonial stem cells and late-stage male germ cells. We used an approach that combines in vitrodifferentiation and in vivotransplantation. Embryoid bodies （EBs） were obtained from iPS cells using leukaemia inhibitor factor （LIF）-free medium. Quantitative PCR revealed a decrease in Oct4 expression and an increase in StraSand Vasa mRNA in the EBs derived from iPS cells, iPS cell-derived EBs were induced by retinoic acid to differentiate into spermatogonial stem cells （SSCs）, as evidenced by their expression of VASA, as well as CDH1 and GFRal, which are markers of SSCs. Furthermore, these germ cells derived from iPS cells were transplanted into recipient testes of mice that had been pre-treated with busulfan. Notably, iPS cell-derived SSCs were able to differentiate into male germ cells ranging from spermatogonia to round spermatids, as shown by VASA and SCP3 expression. This study demonstrates that iPS cells have the potential to differentiate into late-stage male germ cells. The derivation of male germ cells from iPS cells has potential applications in the treatment of male infertility and provides a model for uncovering the molecular mechanisms underlying male germ cell development.

修饰吡啶衍生物的染料敏化光阳极水氧化性能的研究

染料敏化光电化学电池(DSPECs)是构建人工光合作用体系的潜在方式,其优势在于可通过优化染料结构来拓展可见光吸收范围,从根本上提高太阳能利用效率.染料敏化光阳极在受激发产生电荷分离之后,激发电子注入TiO_(2)半导体导带,由于其导带位置比传统的可见光半导体,如BiVO4和Fe3O4等相比较负,因此理论上可以在较小的偏压下取得较大的光电转换效率,也更有利于和光阴极相耦合实现无偏压分解水.电荷传输动力学研究表明,注入到TiO_(2)导带的电子向氧化态光敏剂和催化剂的回传是造成体系能量损失的主要原因,集中体现在光电流密度和效率的降低.目前,已经报道了多种手段来减少DSPECs光阳极表面的电子回传,包括使用带有长烷基链的锚定基团对水氧化催化剂进行修饰,在半导体表面引入电子中介体以及使用核-壳结构的基底等.其中,SnO2/TiO_(2)基底被广泛应用在染料敏化光阳极中,这种基底可以提高光生电子的注入效率,同时两种金属氧化物之间的异质结有效抑制了电子回传,从而提高了DSPECs的光电活性.然而,核-壳结构基底需要使用原子层沉积技术来制备,所以操作相对复杂.本文基于Ru-bda(bda=2,2'-联吡啶-6,6'-二羧酸)结构的分子水氧化催化剂和带有磷酸修饰基团的三联吡啶钌通过共吸附的方式制备染料敏化光阳极,在不使用核-壳结构基底的情况下,利用吡啶衍生物对TiO_(2)电极表面的修饰来减少电子回传.本文利用一系列吡啶衍生物作修饰负载在TiO_(2)光阳极上(TiO_(2)|RuP,1;RuP=Ru(4,4'-(PO3H2)2-2,2'-联吡啶)(2,2'-联吡啶)2;1=Ru(bda)(L)2,bda=2,2'-联吡啶-6,6'-二羧酸,L=(10-吡啶-4-基氧基)癸基)膦酸.在100 mW/cm2的白光照射下(λ>400 nm),TiO_(2)|RuP,1,P1(P1=4-羟基吡啶)光阳极在0.4 V(vs.NHE)的外加偏压下获得了1 mA/cm2的光电流密度,其光电流比未修饰吡啶的光阳极增加了42%.同时,其入射光子-电流转化效率在470 nm波长的单色光光照下达到最大,为13.6%.经过吡啶衍生物所修饰的光阳极光电性能和文献中利用核-壳结构基底所制备的类似光阳极性能相当,且光电流密度随吡啶对位取代基供电性能的增强而增大.瞬态吸收光谱和电化学阻抗谱测试表明,吡啶吸附在光阳极上能有效地抑制界面上的电子回传,延长电荷分离寿命,是光电流增加的根本原因,这也表明有机小分子修饰是提高染料敏化光阳极性能的简单、有效的策略.

Influence of dominance rank and affiliation relationships on selfdirected behavior in female Tibetan macaques (Macaca thibetana)

Self-directed behavior(SDB) is characterized as an indicator of anxiety, frustration and stress in nonhuman primates. In this study, we collected self-directed behavior data from one group of free-ranging Tibetan macaques(Macaca thibetana) at Mt. Huangshan, China(September 2012–May 2013) using a combination of behavioral sampling methods including focal animal sampling, behavioral sampling, continuous sampling and instantaneous sampling. Our results showed that females engaged in significantly higher rates of self-directed behavior when they were in proximity to dominant individuals compared to subordinate ones. Conflict losers significantly increased their SDB rates after agonistic episodes, indicating that SDB might also serve as an index of anxiety in M. thibetana. We further found that females significantly increased their SDB rates when focal individual was proximity to weakly affiliation relationship higher rank members than to strongly affiliation relationship higher rank members. If conflicts were not reconciled, the postconflict SDB rates of losers were higher when they stayed with strongly affiliation opponents; if conflicts were reconciled, victims of strongly affiliation relationships opponents engaged in more SDB rates before reconciliation than after reconciliation, while victims of moderately affiliation relationships opponents did not engaged in more SDB rates before reconciliation than after reconciliation. We conclude that both of dominance rank and affiliation relationships might both influence the SDB rates of female Tibetan macaques significantly, suggesting that SDB is not only an index of anxiety in Tibetan macaques, but also can provide a new insight into evaluation of social relationships between individuals.

Optimization of Polishing Parameters with Taguchi Method for LBO Crystal in CMP

Chemical mechanical polishing （CMP） was used to polish Lithium triborate （LiB3O5 or LBO） crystal. Taguchi method was applied for optimization of the polishing parameters. Material removal rate （MRR） and surface roughness are considered as criteria for the optimization. The polishing pressure, the abrasive concentration and the table velocity are important parameters which influence MRR and surface roughness in CMP of LBO crystal. Experiment results indicate that for MRR the polishing pressure is the most significant polishing parameter followed by table velocity; while for the surface roughness, the abrasive concentration is the most important one. For high MRR in CMP of LBO ctystal the optimal conditions are： pressure 620 g/cm^2, concentration 5.0 wt pct, and velocity 60 r/min, respectively. For the best surface roughness the optimal conditions are： pressure 416 g/cm^2, concentration 5.0 wt pct, and velocity 40 r/min, respectively. The contributions of individual parameters for MRR and surface roughness were obtained.

Male mate choice in Tibetan macaques Macaca thibetana at Mt.Huangshan,China

Though females are generally more selective in mate choice, males may also benefit from mate choice if male reproductivesuccess is limited by factors other than simply the number of female mates, and if females differ in short-term reproductivepotential. We studied male mate choice in a free-ranging troop of Tibetan macaques Macaco thibetana at Mt. Huangshan,China, from August 2007 to April 2008. We employed focal animal sampling and all occurrence sampling to record sexual relatedbehaviors. Eight adult females were divided into three female quality categories according to the females' age, rank and parity.Using male mating effort as a proxy for male mate choice, we found that males do distinguish female quality and showtime-variant mating strategies. Specifically, females with dominant rank, high fecundity, and middle age attracted significantlymore males. Our results suggest that female short-term reproductive potential appears to be an important variable in determiningmale mating effort. Male Tibetan macaques do exercise mate choice for higher quality females as well as reduce useless reproductivecost, which is consistent with the direct benefits theory of mate

Fabrication of chitosan microspheres for efficient adsorption of methyl orange

In this article, morphology, structure and size controllable chitosan microspheres with high mechanical strength were synthesized by microfluidic technology combining chemical crosslinking and used as an adsorbent for methyl orange. The synthesized adsorbents were characterized using scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FTIR), and an Energy Dispersive Spectrometer(EDS). The effect of pH revealed that the adsorption process depended on pH and the pH variation of methyl orange solution after adsorption indicated that adsorption capacity was affected through the associated role of chitosan nature and pH variation. Experimental results suggested that the as-prepared chitosan microspheres were controlled within a narrow size distribution(coefficients of variation is 1.81%), whose adsorption capacity reached to 207 mg·g^(-1) and mechanical strength was suitable to resist forces. In addition, the adsorption isotherm was well fitted with the Langmuir model, and the adsorption kinetic was best described by the pseudo-second-order kinetic model.The high performance microfluidic-synthesized chitosan microspheres have promising potentials in the applications of removing dyes from wastewater.

Analysis of factors influencing skip lymphatic metastasis in pN_2 non-small cell lung cancer

Objective： Although many clinical studies on skip lymphatic metastasis in non-small cell lung cancer have been reported, the risk factors for skip lymphatic metastasis are still controversy and debatable. This study investigated, by multivariate logistic regression analysis, the clinical features of skip metastasis to mediastinal lymph nodes （N2） in non-small cell lung cancer 0NISCLC） patients. Methods： We collected the clinicopathological data of 256 pN2-NSCLC patients who underwent lobectomy plus systemic lymph node dissection in Fujian Medical University Union Hospital. The cases in the present study were divided into two groups： skip metastasis （N2 skip＋） and non- skip metastasis （N2 skip-）. A retrospective analysis of clinical pathological features of two groups was performed, rib determine an independent factor, multivariate logistic regression analysis was used to identify possible risk factors. Results： A total of 256 pN2-NSCLC patients were recruited. The analysis results showed that gender, pathologic types, surgery, pleural involvement, smoking history, age, tumor stages, and differentiation were not statistical significant factors impacting on skip metastasis in pN2-NSCLC （P〉0.05）, whereas tumor size was an independent factor for skip metastasis （P=0.02）. Conclusions： The rate of skip lymphatic metastasis increases in pN2-NSCLC patients, in accompany with an increased tumor size.

Recurrence of sigmoid colon cancer–derived anal metastasis:A case report and review of literature

BACKGROUND Distant metastasis of colorectal cancer to the anus is very rare,with only 30 related cases published in PubMed thus far.Therefore,recurrence of colorectal cancer derived anus metastases is rarely seen and less presented.CASE SUMMARY Here we report an 80-year-old male patient who underwent radical resection for sigmoid colon cancer in January 2010 and another surgery for anal fistula resection in December 2010.Postoperative pathology of the anal fistula revealed a metastatic moderately differentiated adenocarcinoma.The patient subsequently received chemotherapy and radiotherapy.In May 2020,after the patient reported symptoms of anal swelling and pain,computed tomography and magnetic resonance imaging revealed a perianal abscess.Perianal mass biopsy was performed,and the postoperative pathological diagnosis was metastatic moderately differentiated adenocarcinoma.CONCLUSION This case highlights that there is a risk of recurrence of anal metastasis of colorectal cancer even after 10 years of follow-up.We also reviewed the literature and discuss potential mechanisms for anal metastasis of colorectal cancer,thus providing some suggestions for treatment of these cases.

Knockout of MicroRNA-155 Ameliorates the Th17/Th9 Immune Response and Promotes Wound Healing

MiRNAs are integral for maintaining immune homeostasis and self-tolerance.In this study,qPCR analyses were performed to determine which miRNAs play an important role in wound healing.Next,an experiment in a model of wound healing was performed,and histology,mRNA expression and T-cell subpopulations in wound tissue were analyzed.The accelerated experiments were performed by local injection of either rIL-17A and/or rlL-9 after wound healing.In vitro,the differentiation of Thl7/Th9 in miR-155^+/+ or miR-155^-/- mice was investigated,and the target genes of miR155 were analyzed.From our findings,miR-155^-/- in mice promoted wound healing and weakened T cell-mediated inflammation,especially in IL-17/IL-9,and less severe skin fibrosis developed in the mice.rIL-17A and/or rIL-9 could exacerbate inflammatory injury and delay wound healing.We also demonstrated that miR-155^-/- led to a defect in the differentiation of Th17/Th9 in vitro,and this effect of IL-17/IL-9 might be related to the expression of C-maf,which is a target gene of miR155.MiR-155 regulated IL-17/IL-9-related inflammation in wound healing and might be a potential therapeutic target to attenuate the inflammatory response in wound tissue and promote the closure of wound injuries.

Modified subintimal plaque modification improving future recanalization of chronic total occlusion percutaneous coronary intervention

Background Subintimal plaque modification(SPM) is often performed to restore antegrade flow and facilitate subsequent lesion recanalization. This study aimed to compare the safety and efficacy of modified SPM with traditional SPM. Methods A total of 1454 consecutive patients who failed a chronic total occlusion percutaneous coronary intervention(CTO PCI) attempt and underwent SPM from January 2015 to December 2019 at our hospital were reviewed retrospectively. Fifty-four patients who underwent SPM finally were included in this study. We analyzed the outcomes of all the patients, and the primary endpoint was recanalization rate, which was defined as Thrombolysis in Myocardial Infarction(TIMI) grades 2-3 flow on angiography 30 to 90 days post-procedure. Results The baseline characteristics were similar between the two groups. In the follow-up, the recanalization rate was noticeably higher in the modified SPM group compared with the traditional SPM group(90.9% vs. 62.5%, P < 0.05). The proposed strategy in the modified group was more aggressive, including a larger balloon size(1.83 ± 0.30 vs. 2.48 ± 0.26 mm, P < 0.05) and longer subintimal angioplasty(0.59 ± 0.16 vs. 0.92 ± 0.12 mm, P < 0.05). Also, the common use of a Stingray balloon and guide catheter extension resulted in improvement of patients in the modified SMP group(12.5% vs. 100%, P < 0.05). Conclusion Modified SPM, which is associated with a high likelihood of successful recanalization, is an effective and safe CTO PCI bail out strategy.