Objective To study the chemical constituents of whole plant of Phyllanthus urinaria and their biological activity.Methods The chemical constituents were isolated and purified by repeated column chromatography over sil...Objective To study the chemical constituents of whole plant of Phyllanthus urinaria and their biological activity.Methods The chemical constituents were isolated and purified by repeated column chromatography over silica gel,Rp-C18（reverse phase）,MCI,and Sephadex LH-20,as well as semi-preparative HPLC.NMR spectroscopic analyses were used for the structure identification.In this case,the inhibitory rate of NO production of the isolated triterpenoids was evaluated.Results Seven triterpenoids,identified as28-norlup-20（29）-ene-3,17β-diol（1）,betulin（2）,β-betulinic acid（3）,3-oxofriedelan-28-oic acid（4）,oleanolic acid（5）,3R-E-coumaroyltaraxerol（6）,and 3R-Zcoumaroyltaraxerol（7）,were isolated and identified from the whole plants of P.urinaria.Compounds 1-5 exerted inhibitory effects against NO production in LPS-induced RAW264.7 mouse macrophages with the inhibitory rate of NO production ranging from 4.0%to 27.8%at the concentration of 25 μmol/L.Conclusion To the best of our knowledge,this is the first report of compounds 1-4,6,and 7 from the family Euphorbiaceae.Compounds 1-5 exhibited inhibitory effects against NO production in LPS-induced RAW264.7 mouse macrophages.展开更多
Twenty-one benzoylated phloroglucinol derivatives bearing homoadamantyl frameworks were isolated from the aerial parts of Hypericum sampsonii,including two new ones named hyperisampsins N and O（1 and 2）.The structur...Twenty-one benzoylated phloroglucinol derivatives bearing homoadamantyl frameworks were isolated from the aerial parts of Hypericum sampsonii,including two new ones named hyperisampsins N and O（1 and 2）.The structures of 1 and 2 were elucidated by extensive NMR and mass spectrometric analyses.Their absolute configurations were further determined by using TDDFT ECD calculations.Compounds 2,7,and 8 were evaluated for their cytotoxic activities against five human cancer cell lines,of which,2 and 8 exhibited significant cytotoxic activities toward HL-60 cell and moderate activities against others cell lines.展开更多
Two pairs of chlorine-containing phenylpropanoid enantiomers（1a/1b and 2a/2b） were isolated from the rhizomes of Acorus tatarinowii. Interestingly, these optical isomers（1a/1b and 2a/2b） were co-existed in the sam...Two pairs of chlorine-containing phenylpropanoid enantiomers（1a/1b and 2a/2b） were isolated from the rhizomes of Acorus tatarinowii. Interestingly, these optical isomers（1a/1b and 2a/2b） were co-existed in the same plant, which were characterized as the first halogen-containing natural products from the genus Acorus. Their structures and absolute configurations were elucidated by a combination of spectroscopic analysis and a single-crystal X-ray diffraction, assisted by a modified Mosher＇s method. The phenylpropanoid isomers（1a/1b and 2a/2b） were evaluated for their antioxidant activities using DPPH assay and cytotoxic activities against five human cancer cell lines.展开更多
基金New Century Excellent Talents in University,State Education Ministry of China(No.NCET-2008-0224)National Natural Science Foundation of China(No.31200258,81641129)Fundamental Research Funds for Central Universities(No.2012QN012)
文摘Objective To study the chemical constituents of whole plant of Phyllanthus urinaria and their biological activity.Methods The chemical constituents were isolated and purified by repeated column chromatography over silica gel,Rp-C18（reverse phase）,MCI,and Sephadex LH-20,as well as semi-preparative HPLC.NMR spectroscopic analyses were used for the structure identification.In this case,the inhibitory rate of NO production of the isolated triterpenoids was evaluated.Results Seven triterpenoids,identified as28-norlup-20（29）-ene-3,17β-diol（1）,betulin（2）,β-betulinic acid（3）,3-oxofriedelan-28-oic acid（4）,oleanolic acid（5）,3R-E-coumaroyltaraxerol（6）,and 3R-Zcoumaroyltaraxerol（7）,were isolated and identified from the whole plants of P.urinaria.Compounds 1-5 exerted inhibitory effects against NO production in LPS-induced RAW264.7 mouse macrophages with the inhibitory rate of NO production ranging from 4.0%to 27.8%at the concentration of 25 μmol/L.Conclusion To the best of our knowledge,this is the first report of compounds 1-4,6,and 7 from the family Euphorbiaceae.Compounds 1-5 exhibited inhibitory effects against NO production in LPS-induced RAW264.7 mouse macrophages.
基金financially supported by the Program for New Century Excellent Talents in University,State Education Ministry of China(No.NCET-2008-0224)National Science and Technology Project of China(No.2011ZX09102-004)+2 种基金the National Natural Science Foundation of China(Nos.31370372,81573316,81641129 and 21502057)the Fundamental Research Fund for the Central Universities(No.2016YXMS149)the China Postdoctoral Science Foundation(No.2015M572154)
文摘Twenty-one benzoylated phloroglucinol derivatives bearing homoadamantyl frameworks were isolated from the aerial parts of Hypericum sampsonii,including two new ones named hyperisampsins N and O（1 and 2）.The structures of 1 and 2 were elucidated by extensive NMR and mass spectrometric analyses.Their absolute configurations were further determined by using TDDFT ECD calculations.Compounds 2,7,and 8 were evaluated for their cytotoxic activities against five human cancer cell lines,of which,2 and 8 exhibited significant cytotoxic activities toward HL-60 cell and moderate activities against others cell lines.
基金financially supported by the Program for New Century Excellent Talents in University, State Education Ministry of China (No. NCET2008-0224)the National Natural Science Foundation of China (Nos. 31370372, 81573316, 31570361, 31200258)
文摘Two pairs of chlorine-containing phenylpropanoid enantiomers（1a/1b and 2a/2b） were isolated from the rhizomes of Acorus tatarinowii. Interestingly, these optical isomers（1a/1b and 2a/2b） were co-existed in the same plant, which were characterized as the first halogen-containing natural products from the genus Acorus. Their structures and absolute configurations were elucidated by a combination of spectroscopic analysis and a single-crystal X-ray diffraction, assisted by a modified Mosher＇s method. The phenylpropanoid isomers（1a/1b and 2a/2b） were evaluated for their antioxidant activities using DPPH assay and cytotoxic activities against five human cancer cell lines.
