Abiotic stress is one of the most important factors reducing soybean yield. It is essential to identify regulatory factors contributing to stress responses.A previous study found that the tandem CCCH zincfinger protei...Abiotic stress is one of the most important factors reducing soybean yield. It is essential to identify regulatory factors contributing to stress responses.A previous study found that the tandem CCCH zincfinger protein Gm ZF351 is an oil level regulator. In this study, we discovered that the Gm ZF351 gene is induced by stress and that the overexpression of Gm ZF351 confers stress tolerance to transgenic soybean. Gm ZF351 directly regulates the expression of Gm CIPK9 and Gm SnRK, leading to stomata closing, by binding to their promoter regions, which carry two CT(G/C)(T/A)AA elements.Stress induction of Gm ZF351 is mediated through reduction in the H3K27me3 level at the Gm ZF351locus.TwoJMJ30-demethylase-likegenes,Gm JMJ30-1 and Gm JMJ30-2, are involved in this demethylationprocess.Overexpressionof Gm JMJ30-1/2 in transgenic hairy roots enhances Gm ZF351 expression mediated by histone demethylation and confers stress tolerance to soybean.Yield-related agronomic traits were evaluated in stable Gm ZF351-transgenic plants under mild drought stress conditions. Our study reveals a new mode of Gm JMJ30-Gm ZF351 action in stress tolerance, in addition to that of Gm ZF351 in oil accumulation. Manipulation of the components in this pathway is expected to improve soybean traits and adaptation under unfavorable environments.展开更多
Seed weight is usually associated with seed size and is one of the important agronomic traits that determine yield.Understanding of seed weight control is limited,especially in soybean plants.Here we show that Glycine...Seed weight is usually associated with seed size and is one of the important agronomic traits that determine yield.Understanding of seed weight control is limited,especially in soybean plants.Here we show that Glycine max JASMONATEZIM DOMAIN 3(GmJAZ3),a gene identified through gene co-expression network analysis,regulates seed-related traits in soybean.Overexpression of GmJAZ3 promotes seed size/weight and other organ sizes in stable transgenic soybean plants likely by increasing cell proliferation.GmJAZ3 interacted with both G.max RESPONSE REGULATOR 18a(GmRR18a)and GmMYC2a to inhibit their transcriptional activation of cytokinin oxidase gene G.max CYTOKININ OXIDASE 3-4(GmCKX3-4),which usually affects seed traits.Meanwhile,the GmRR18a binds to the promoter of GmMYC2a and activates GmMYC2a gene expression.In GmJAZ3-overexpressing soybean seeds,the protein contents were increased while the fatty acid contents were reduced compared to those in the control seeds,indicating that the GmJAZ3 affects seed size/weight and compositions.Natural variation in JAZ3 promoter region was further analyzed and Hap3 promoter correlates with higher promoter activity,higher gene expression and higher seed weight.The Hap3 promoter may be selected and fixed during soybean domestication.JAZ3 orthologs from other plants/crops may also control seed size and weight.Taken together,our study reveals a novel molecular module GmJAZ3-GmRR18a/GmMYC2a-GmCKXs for seed size and weight control,providing promising targets during soybean molecular breeding for better seed traits.展开更多
The soybean aphid,Aphis glycines,is an extreme specialist and an important invasive pest that relies on olfaction for behaviors such as feeding,mating,and foraging.Odorant-binding proteins(OBPs)play a vital role in ol...The soybean aphid,Aphis glycines,is an extreme specialist and an important invasive pest that relies on olfaction for behaviors such as feeding,mating,and foraging.Odorant-binding proteins(OBPs)play a vital role in olfaction by binding to volatile compounds and by regulating insect sensing of the environment.In this work we used rapid amplification of complementary DNA ends technology to identify and characterize 10 genes encoding A.glycines OBPs(AglyOBPs)belonging to 3 subfamilies,including 4 classic OBPs,5 Plus-C OBPs,and one Minus-C OBP.Quantitative real-time polymerase chain reaction demonstrated variable specific expression patterns for the 10 genes based on developmental stage and aphid tssue sampled.Expression levels of 7 AglyOBPs(2,3,4,5,7,9,and 10)were highest in the 4th instar,indicating that the 4th nymphal instar is an important developmental period during which soybean aphids regulate feeding and search for host plants.Tissue-specific expression results demonstrated that AglyOBP2,7,and 9 exhibited significantly higher expression levels in antennae.Meanwhile,ligand-binding analysis of5 OBPs demonstrated binding of AglyOBP2 and AglyOBP3 to a broad spectrum of volatiles released by green leaf plants,with bias toward 6-to 8-carbon chain volatiles and strong binding of AglyOBP7 to trans-B-farnesene.Taken together,our findings build a foundation of knowledge for use in the study of molecular olfaction mechanisms and prov ide insights to guide future soybean aphid research.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. U1906203, 32101676, 32171930, 31671258, 31971896)the Chinese Academy of Science (CAS) Key Project (ZDRW-ZS-2019-2)+1 种基金the Key R&D Project (Grant No. 2019YFD1002701)State Key Lab of Plant Genomics, IGDB, CAS。
文摘Abiotic stress is one of the most important factors reducing soybean yield. It is essential to identify regulatory factors contributing to stress responses.A previous study found that the tandem CCCH zincfinger protein Gm ZF351 is an oil level regulator. In this study, we discovered that the Gm ZF351 gene is induced by stress and that the overexpression of Gm ZF351 confers stress tolerance to transgenic soybean. Gm ZF351 directly regulates the expression of Gm CIPK9 and Gm SnRK, leading to stomata closing, by binding to their promoter regions, which carry two CT(G/C)(T/A)AA elements.Stress induction of Gm ZF351 is mediated through reduction in the H3K27me3 level at the Gm ZF351locus.TwoJMJ30-demethylase-likegenes,Gm JMJ30-1 and Gm JMJ30-2, are involved in this demethylationprocess.Overexpressionof Gm JMJ30-1/2 in transgenic hairy roots enhances Gm ZF351 expression mediated by histone demethylation and confers stress tolerance to soybean.Yield-related agronomic traits were evaluated in stable Gm ZF351-transgenic plants under mild drought stress conditions. Our study reveals a new mode of Gm JMJ30-Gm ZF351 action in stress tolerance, in addition to that of Gm ZF351 in oil accumulation. Manipulation of the components in this pathway is expected to improve soybean traits and adaptation under unfavorable environments.
基金supported by the National Key R&D Project(2021YFF1001201,2021YFF1000104-2)the National Natural Science Foundation of China(32090062,32090063)+2 种基金the Chinese Academy of Science leading project(XDA24010105)the State Key Lab of Plant Genomicsthe Qilu Zhongke Academy of Modern Microbiology Technology。
文摘Seed weight is usually associated with seed size and is one of the important agronomic traits that determine yield.Understanding of seed weight control is limited,especially in soybean plants.Here we show that Glycine max JASMONATEZIM DOMAIN 3(GmJAZ3),a gene identified through gene co-expression network analysis,regulates seed-related traits in soybean.Overexpression of GmJAZ3 promotes seed size/weight and other organ sizes in stable transgenic soybean plants likely by increasing cell proliferation.GmJAZ3 interacted with both G.max RESPONSE REGULATOR 18a(GmRR18a)and GmMYC2a to inhibit their transcriptional activation of cytokinin oxidase gene G.max CYTOKININ OXIDASE 3-4(GmCKX3-4),which usually affects seed traits.Meanwhile,the GmRR18a binds to the promoter of GmMYC2a and activates GmMYC2a gene expression.In GmJAZ3-overexpressing soybean seeds,the protein contents were increased while the fatty acid contents were reduced compared to those in the control seeds,indicating that the GmJAZ3 affects seed size/weight and compositions.Natural variation in JAZ3 promoter region was further analyzed and Hap3 promoter correlates with higher promoter activity,higher gene expression and higher seed weight.The Hap3 promoter may be selected and fixed during soybean domestication.JAZ3 orthologs from other plants/crops may also control seed size and weight.Taken together,our study reveals a novel molecular module GmJAZ3-GmRR18a/GmMYC2a-GmCKXs for seed size and weight control,providing promising targets during soybean molecular breeding for better seed traits.
基金We gratefully acknowledge Dr.Tao Zhong(Shenyang Academy of Agricultural Sciences,China)for his con-structive reviews of the manuscript.This、work was supported by the Natural Science Foundation of Hei-longjiang(C2018060)Heilongjiang Postdoctoral Fund(LBH-Z16187)+2 种基金Scientific Research Project of Hei-longjiang Academy of Agricultural Sciences(2017ZC10,2017SJ032 and 2018JJPY004)National Key Research and Development Program(2017YFE0111000),National Natural Science Foundation ofChina(31771823)Ma-jor Project of Research and Development of Applied Tech-nology in Heilongjiang Province(GA18B101).
文摘The soybean aphid,Aphis glycines,is an extreme specialist and an important invasive pest that relies on olfaction for behaviors such as feeding,mating,and foraging.Odorant-binding proteins(OBPs)play a vital role in olfaction by binding to volatile compounds and by regulating insect sensing of the environment.In this work we used rapid amplification of complementary DNA ends technology to identify and characterize 10 genes encoding A.glycines OBPs(AglyOBPs)belonging to 3 subfamilies,including 4 classic OBPs,5 Plus-C OBPs,and one Minus-C OBP.Quantitative real-time polymerase chain reaction demonstrated variable specific expression patterns for the 10 genes based on developmental stage and aphid tssue sampled.Expression levels of 7 AglyOBPs(2,3,4,5,7,9,and 10)were highest in the 4th instar,indicating that the 4th nymphal instar is an important developmental period during which soybean aphids regulate feeding and search for host plants.Tissue-specific expression results demonstrated that AglyOBP2,7,and 9 exhibited significantly higher expression levels in antennae.Meanwhile,ligand-binding analysis of5 OBPs demonstrated binding of AglyOBP2 and AglyOBP3 to a broad spectrum of volatiles released by green leaf plants,with bias toward 6-to 8-carbon chain volatiles and strong binding of AglyOBP7 to trans-B-farnesene.Taken together,our findings build a foundation of knowledge for use in the study of molecular olfaction mechanisms and prov ide insights to guide future soybean aphid research.