BACKGROUND: Ultrasound-guided transversus abdominis plane (TAP) block is an adjunct therapy to provide effective postoperative analgesia in abdominal surgical procedures. Dexamethasone is a supplement agent that can i...BACKGROUND: Ultrasound-guided transversus abdominis plane (TAP) block is an adjunct therapy to provide effective postoperative analgesia in abdominal surgical procedures. Dexamethasone is a supplement agent that can improve the efifcacy of local anesthesia. However, information about its additive effect is limited. This study aimed to compare the an-algesic efifciency using ultrasound-guided TAP block with and without perineural dexamethasone for patients who under-went laparoscopic cholecystectomy. METHODS: Sixty patients who underwent laparoscopic cho-lecystectomy were randomly divided into three groups: group I, controls; group II, TAP; and group III, TAP+perineural dexamethasone supplement. The requirement of additional analgesia and the ifrst-time request of rescue-analgesia were recorded after operation and the numerical rating scale was evaluated at speciifc intervals. RESULTS: Compared to group I, the ifrst-time requirement of rescue-analgesia in groups II and III was signiifcantly delayed (403.0±230.9, 436.0±225.3 vs 152.3±124.7,P<0.01). Compared with those in group I, patients in groups II and III were associ-ated with lower numerical rating scale pain scores (P<0.01) and less postoperative analgesic consumption (P<0.01). There was no signiifcant difference in the variables mentioned above between groups II and III (P>0.05). CONCLUSION: Perineural dexamethasone has no additive/synergistic effect with subcostal TAP block on analgesic efif-cacy for the patients undergoing laparoscopic cholecystectomy.展开更多
BACKGROUND Metastasis of pancreatic cancer to the colon is rare and the features need to be further elucidated.Herein,we report a rare case of pancreatic cancer with simultaneous liver and colon metastases.CASE SUMMAR...BACKGROUND Metastasis of pancreatic cancer to the colon is rare and the features need to be further elucidated.Herein,we report a rare case of pancreatic cancer with simultaneous liver and colon metastases.CASE SUMMARY A 48-year-old man with intrahepatic space-occupying lesions based on a computed tomography scan was admitted to our hospital for further treatment.Abdominal magnetic resonance imaging revealed a 6.4 cm×4.2 cm mass in the tail of the pancreas and multiple low-density masses in the liver parenchyma.In addition,a mass of 2.2 cm×1.6 cm with surface congestive erosions in the sigmoid colon was detected by colonoscopy.Histopathological examination of biopsies from both the liver and colon lesions revealed a moderately to poorly differentiated adenocarcinoma.Immunohistochemical staining of the colon tumor was positive for cytokeratin(CK)7 and CK,but negative for colorectal adenocarcinoma-related markers CK 20,CDX2,and SATB2,thus indicating that the metastasis originated from the pancreas.Next-generation sequencing for genomic profiling of the liver and colon metastases both found mutations in KRAS(p.G12D)and TP53(c.376-1delG),with microsatellite stable and low tumor mutational burden without actionable or cancer-predisposing gene mutations detected.The patient was subsequently treated with 12 cycles of FOLFIRINOX which led to a sustainable response,followed by ongoing maintenance treatment with irinotecan plus fluorouracil.CONCLUSION For this rare case,careful evaluation of histopathological and immunohistochemical staining results are required.The genomic profiling of colon lesions was revealed for the first time,and FOLFIRINOX showed good treatment efficacy in this patient.展开更多
Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utili...Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression (SAGE) revealed 1183 differentially expressed genes (DGs), among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F1 hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F1 hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci (QTL), a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.展开更多
Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice (Oryza sativa L.), cD...Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice (Oryza sativa L.), cDNAs of three putative cell- wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were isolated. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing caryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the caryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 in embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected in the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcript was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross-cells, the aleurone layer and the nuceUar tissue. These data indicate that the three cell-wall invertase genes play complementary/synergetic roles in assimilate unloading during the grain filling stage. In addition, the cell type-specific expression patterns of OsCIN3 in source leaf blades and anthers were also investigated, and its corresponding physiological roles were discussed.展开更多
In view of the high energy consumption and low response speed of the traditional hydraulic system for an injection molding machine,a servo motor driven constant pump hydraulic system is designed for a precision inject...In view of the high energy consumption and low response speed of the traditional hydraulic system for an injection molding machine,a servo motor driven constant pump hydraulic system is designed for a precision injection molding process,which uses a servo motor,a constant pump,and a pressure sensor,instead of a common motor,a constant pump,a pressure proportion valve,and a flow proportion valve.A model predictive control strategy based on neurodynamic optimization is proposed to control this new hydraulic system in the injection molding process.Simulation results showed that this control method has good control precision and quick response.展开更多
Understanding of charge/energy exchange processes and interfacial interactions that occur between quantum dots (QDs) and the metal oxides is of critical importance to these QD-based optoelectronic devices. This work r...Understanding of charge/energy exchange processes and interfacial interactions that occur between quantum dots (QDs) and the metal oxides is of critical importance to these QD-based optoelectronic devices. This work reports on linear dipole behavior of single near-infrared emitting CdSeTe/ZnS core/shell QDs which are encased in indium tin oxide (ITO) semiconductor lianoparticles films. A strong polarization anisotropy in photohiminescence emission is observed by defocused wide-field imaging and polarization measuremen11echniques, and the average polarization degree is up to 0.45. A possible mechanism for the observation is presented in which the electrons, locating at single QD surface from ITO by electron transfer due to the equilibration of the Fermi levels, result in a significant Stark distortion of the QD electron/hole wavefunctions. The Stark distortion results in the linear polarization property of the single QDs. The investigation of linear dipole behavior for single QDs encased in ITO films would be helpful for further improving QD-based device performance.展开更多
We report an experimental investigation of the influence of surface charges on the emission polarization properties of single CdSe/CdS dot-in-rods(DRs),which is important for their polarization-based practical applica...We report an experimental investigation of the influence of surface charges on the emission polarization properties of single CdSe/CdS dot-in-rods(DRs),which is important for their polarization-based practical applications.By covering the single DRs with N-type semiconductor indium tin oxide(ITO)nanoparticles,the surface of single DRs is charged by ITO through interracial electron transfer.This is confirmed by the experimental observations of the reduced photoluminescence intensities and lifetimes as well as the suppressing blinking.It is found that the full width at half maximum of histogram of polarization degrees of the single DRs is broadened from 0.24(on glass)to 0.41(in ITO).In order to explain the exprimental results,the band-edge exciton fine structure of single DRs is calculated by taking into account the sample parameters,the emission polarization,and the surface charges.The calculation results show that the level ordering of the emitting states determines the polarization degrees tending to increase or decrease under the influence of surface electrons.The surface electrons can induce an increase in the spacing between the emitting levels to change the populations and thus change the polarization degrees.In addition,different numbers of surface electrons may randomly distribute on the long CdSe/CdS rods,leading to the heterogeneous influences on the single DRs causing the broadening of polarization degrees also.展开更多
基金Projects(51871195,51501015)supported by the National Natural Science Foundation of ChinaProject(TC170A5SU-1)supported by Ministry of Industry and Information Technology of China。
基金Projects(51871195,51501015) supported by the National Natural Science Foundation of ChinaProject(TC170A5SU-1) supported by Ministry of Industry and Information Technology,China
文摘BACKGROUND: Ultrasound-guided transversus abdominis plane (TAP) block is an adjunct therapy to provide effective postoperative analgesia in abdominal surgical procedures. Dexamethasone is a supplement agent that can improve the efifcacy of local anesthesia. However, information about its additive effect is limited. This study aimed to compare the an-algesic efifciency using ultrasound-guided TAP block with and without perineural dexamethasone for patients who under-went laparoscopic cholecystectomy. METHODS: Sixty patients who underwent laparoscopic cho-lecystectomy were randomly divided into three groups: group I, controls; group II, TAP; and group III, TAP+perineural dexamethasone supplement. The requirement of additional analgesia and the ifrst-time request of rescue-analgesia were recorded after operation and the numerical rating scale was evaluated at speciifc intervals. RESULTS: Compared to group I, the ifrst-time requirement of rescue-analgesia in groups II and III was signiifcantly delayed (403.0±230.9, 436.0±225.3 vs 152.3±124.7,P<0.01). Compared with those in group I, patients in groups II and III were associ-ated with lower numerical rating scale pain scores (P<0.01) and less postoperative analgesic consumption (P<0.01). There was no signiifcant difference in the variables mentioned above between groups II and III (P>0.05). CONCLUSION: Perineural dexamethasone has no additive/synergistic effect with subcostal TAP block on analgesic efif-cacy for the patients undergoing laparoscopic cholecystectomy.
文摘BACKGROUND Metastasis of pancreatic cancer to the colon is rare and the features need to be further elucidated.Herein,we report a rare case of pancreatic cancer with simultaneous liver and colon metastases.CASE SUMMARY A 48-year-old man with intrahepatic space-occupying lesions based on a computed tomography scan was admitted to our hospital for further treatment.Abdominal magnetic resonance imaging revealed a 6.4 cm×4.2 cm mass in the tail of the pancreas and multiple low-density masses in the liver parenchyma.In addition,a mass of 2.2 cm×1.6 cm with surface congestive erosions in the sigmoid colon was detected by colonoscopy.Histopathological examination of biopsies from both the liver and colon lesions revealed a moderately to poorly differentiated adenocarcinoma.Immunohistochemical staining of the colon tumor was positive for cytokeratin(CK)7 and CK,but negative for colorectal adenocarcinoma-related markers CK 20,CDX2,and SATB2,thus indicating that the metastasis originated from the pancreas.Next-generation sequencing for genomic profiling of the liver and colon metastases both found mutations in KRAS(p.G12D)and TP53(c.376-1delG),with microsatellite stable and low tumor mutational burden without actionable or cancer-predisposing gene mutations detected.The patient was subsequently treated with 12 cycles of FOLFIRINOX which led to a sustainable response,followed by ongoing maintenance treatment with irinotecan plus fluorouracil.CONCLUSION For this rare case,careful evaluation of histopathological and immunohistochemical staining results are required.The genomic profiling of colon lesions was revealed for the first time,and FOLFIRINOX showed good treatment efficacy in this patient.
基金Project supported by the National Natural Science Foundation of China (No. 51377142), the National High-Tech R&D Program (863) of China (No. 2014AA052001), the Zhejiang Provincial Natural Science Foundation of China (No. LY16E070002), and the Zhejiang Province Key R&D Project (No. 2017C01039)
文摘Heterosis is a biological phenomenon whereby the offspring from two parents show improved and superior performance than either inbred parental lines. Hybrid rice is one of the most successful apotheoses in crops utilizing heterosis. Transcriptional profiling of F1 super-hybrid rice Liangyou-2186 and its parents by serial analysis of gene expression (SAGE) revealed 1183 differentially expressed genes (DGs), among which DGs were found significantly enriched in pathways such as photosynthesis and carbon-fixation, and most of the key genes involved in the carbon-fixation pathway exhibited up-regulated expression in F1 hybrid rice. Moreover, increased catabolic activity of corresponding enzymes and photosynthetic efficiency were also detected, which combined to indicate that carbon fixation is enhanced in F1 hybrid, and might probably be associated with the yield vigor and heterosis in super-hybrid rice. By correlating DGs with yield-related quantitative trait loci (QTL), a potential relationship between differential gene expression and phenotypic changes was also found. In addition, a regulatory network involving circadian-rhythms and light signaling pathways was also found, as previously reported in Arabidopsis, which suggest that such a network might also be related with heterosis in hybrid rice. Altogether, the present study provides another view for understanding the molecular mechanism underlying heterosis in rice.
基金the State Key Basic Research and Development Plan ofChina (2004CB720406)the Innovation Foundation of the Chinese Academyof Sciences (KSCX2-SW-306 and KSCX1-SW-03)the Program for Strategic Scientific Alliances between China and the Netherlands (KNAW-PSA, 04-PSA-BD-04 for P.B.F.O., KNAW-CEP and 04CDP022 for Y.X.)
文摘Cell-wall invertase plays an important role in sucrose partitioning between source and sink organs in higher plants. To investigate the role of cell-wall invertases for seed development in rice (Oryza sativa L.), cDNAs of three putative cell- wall invertase genes OsCIN1, OsCIN2 and OsCIN3 were isolated. Semi-quantitative reverse transcription-polymerase chain reaction analysis revealed different expression patterns of the three genes in various rice tissues/organs. In developing caryopses, they exhibited similar temporal expression patterns, expressed highly at the early and middle grain filling stages and gradually declined to low levels afterward. However, the spatial expression patterns of them were very different, with OsCIN1 primarily expressed in the caryopsis coat, OsCIN2 in embryo and endosperm, and OsCIN3 in embryo. Further RNA in situ hybridization analysis revealed that a strong signal of OsCIN2 mRNA was detected in the vascular parenchyma surrounding the xylem of the chalazal vein and the aleurone layer, whereas OsCIN3 transcript was strongly detected in the vascular parenchyma surrounding the phloem of the chalazal vein, cross-cells, the aleurone layer and the nuceUar tissue. These data indicate that the three cell-wall invertase genes play complementary/synergetic roles in assimilate unloading during the grain filling stage. In addition, the cell type-specific expression patterns of OsCIN3 in source leaf blades and anthers were also investigated, and its corresponding physiological roles were discussed.
基金Project supported by the National Natural Science Foundation of China(No.61203299)the Fundamental Research Funds for the Central Universities(No.2013QNA4021)+1 种基金the Natural Science Foundation of Zhejiang Province(Nos.Y1110135 and LY12F03018)the Qianjiang Talents Program of Zhejiang Province,China(No.2013R10047)
文摘In view of the high energy consumption and low response speed of the traditional hydraulic system for an injection molding machine,a servo motor driven constant pump hydraulic system is designed for a precision injection molding process,which uses a servo motor,a constant pump,and a pressure sensor,instead of a common motor,a constant pump,a pressure proportion valve,and a flow proportion valve.A model predictive control strategy based on neurodynamic optimization is proposed to control this new hydraulic system in the injection molding process.Simulation results showed that this control method has good control precision and quick response.
基金the National Key R&D Program of China (No. 2017YFA0304203)the National Natural Science Foundation of China (Grant Nos. 61527824, 61675119, U1510133, 11434007, 11504216, and 61605104), PCSIRT (No. IRT_17R70)+1 种基金Y. Peng was supported by the National Natural Science Foundation of China (No. 11404189)H. Xie was supported by the National Natural Science Foundation of China (No. 11504260).
文摘Understanding of charge/energy exchange processes and interfacial interactions that occur between quantum dots (QDs) and the metal oxides is of critical importance to these QD-based optoelectronic devices. This work reports on linear dipole behavior of single near-infrared emitting CdSeTe/ZnS core/shell QDs which are encased in indium tin oxide (ITO) semiconductor lianoparticles films. A strong polarization anisotropy in photohiminescence emission is observed by defocused wide-field imaging and polarization measuremen11echniques, and the average polarization degree is up to 0.45. A possible mechanism for the observation is presented in which the electrons, locating at single QD surface from ITO by electron transfer due to the equilibration of the Fermi levels, result in a significant Stark distortion of the QD electron/hole wavefunctions. The Stark distortion results in the linear polarization property of the single QDs. The investigation of linear dipole behavior for single QDs encased in ITO films would be helpful for further improving QD-based device performance.
基金The project was sponsored by the National Key Research and Development Program of China(Grant No.2017YFA0304203)the National Natural Science Foundation of China(Grant Nos.61527824,61675119,11434007,61875109,and 61605104)PCSIRT(Grant No.IRT.17R70),1331KSC and 111 project(Grant No.D18001).
文摘We report an experimental investigation of the influence of surface charges on the emission polarization properties of single CdSe/CdS dot-in-rods(DRs),which is important for their polarization-based practical applications.By covering the single DRs with N-type semiconductor indium tin oxide(ITO)nanoparticles,the surface of single DRs is charged by ITO through interracial electron transfer.This is confirmed by the experimental observations of the reduced photoluminescence intensities and lifetimes as well as the suppressing blinking.It is found that the full width at half maximum of histogram of polarization degrees of the single DRs is broadened from 0.24(on glass)to 0.41(in ITO).In order to explain the exprimental results,the band-edge exciton fine structure of single DRs is calculated by taking into account the sample parameters,the emission polarization,and the surface charges.The calculation results show that the level ordering of the emitting states determines the polarization degrees tending to increase or decrease under the influence of surface electrons.The surface electrons can induce an increase in the spacing between the emitting levels to change the populations and thus change the polarization degrees.In addition,different numbers of surface electrons may randomly distribute on the long CdSe/CdS rods,leading to the heterogeneous influences on the single DRs causing the broadening of polarization degrees also.