Th22 cell accumulation is associated with colorectal cancer development

AIM: To investigate the expression of Th22 cells and related cytokines in colorectal cancer(CRC) tissues, and the probably mechanism.METHODS: CRC tumor and paratumor tissues were collected to detect the expression levels of Th22 cells and of related cytokines by immunohistochemistry, flow cytometry and real-time quantitative polymerase chain reaction(RT-q PCR).Interleukin(IL)-22 alone or with a STAT3 inhibitor was co-cultured with RKO cells in vitro to study the effects of IL-22 on colon cancer cells.IL-22 alone or with a STAT3 inhibitor was injected into a BALB/c nude mouse model with subcutaneously transplanted RKO cells to study the effects of IL-22 on colon cancer growth.RESULTS: The percentage of Th22 cells in the CD4+ T subset was significantly higher in tumor tissues compared with that in paratumor tissues(1.47% ± 0.083% vs 1.23% ± 0.077%, P < 0.05) as determined by flow cytometry.RT-qP CR analysis revealed that the m RNA expression levels of IL-22, aryl hydrocarbon receptor, CCL20 and CCL22 were significantly higher in tumor tissues compared with those in paratumor tissues.CCL27 mR NA also displayed a higher expression level in tumor tissues compared with that in paratumor tissues; however, these levels were not significantly different(2.58 ± 0.93 vs 2.30 ± 0.78, P > 0.05).IL-22 enhanced colon cancer cell proliferation in vitro and displayed anti-apoptotic effects; these effects were blocked by adding a STAT3 inhibitor.IL-22 promoted tumor growth in BALB/c nude mice; however, this effect was reversed by adding a STAT3 inhibitor.CONCLUSION: Th22 cells that accumulate in CRC may be associated with the chemotactic effect of the tumor microenvironment.IL-22 is associated with CRC development, most likely via STAT3 activation.

Model-free adaptive control for three-degree-of-freedom hybrid magnetic bearings

由于气隙磁场分布不均以及未建模动态性,数学模型不再适用于磁力轴承系统。基于错误数学模型的系统控制会表现出低响应和高颤动。为解决这一问题,本文提出一种三自由度混合磁轴承无模型自适应控制系统。该控制系统仅与控制电流和轴承的平衡位置有关。其参数估计算法的设计与模型无关,直接基于输入和输出数据的伪偏导数。本文通过不同直径的辅助轴承分析了三自由度混合磁轴承转子悬浮区域,以及通过两种不同的操作系统具体分析无模型自适应控制系统的可行性。模拟和实验结果表明,该三自由度混合磁轴承无模型自适应控制系统具有响应速度快,稳态误差小,和稳定性好等优势。