AIM: To investigate mitochondrial factors associated with Leber hereditary optic neuropathy (LHON) through complete sequencing and analysis of the mitochondrial genome of Chinese patients with this disease. METHODS: T...AIM: To investigate mitochondrial factors associated with Leber hereditary optic neuropathy (LHON) through complete sequencing and analysis of the mitochondrial genome of Chinese patients with this disease. METHODS: Two unrelated southern Chinese families with LHON and 10 matched healthy controls were recruited, and their entire mitochondrial DNA (mtDNA) was amplified and sequenced with the universal M13 primer. Then DNA sequence analysis and variation identification were performed by DNAssist and Chromas 2 software and compared with authoritative databases such as Mitomap. RESULTS: Mutational analysis of mtDNA in these two Chinese pedigrees revealed one common LHON-associated mutation, G11778A (Arg -> His), in the MT-ND4 gene. In addition, there were two secondary mutations in Pedigree 1: C34971 (Ala -> Val), and C3571T (Leu -> Phe) in the MT-ND1 gene, which have not been reported; and two secondary mutations occurred in Pedigree 2: A10398G (Thr -> Ala) in the MT-ND3 gene, and T14502C (Ile -> Val) in the MT-ND6 gene. Three polymorphisms, A73G, G94A and A263G in the mtDNA control region, were also found. CONCLUSION: Our study confirmed that the known MT-ND4* G11778A mutation is the most significant cause of LHON. The C3497T and C3571T mutations in Pedigree 1 were also both at hot-spots of MT-ND1; they may affect the respiratory chain in coordination with the primary mutation G11778A. In Pedigree 2, the two secondary mutations A10398G of MT-ND3 and T14502C of MT-ND6 may influence mitochondrial respiratory complex I, leading to the mitochondrial respiratory chain dysfunction which results in optic atrophy together with G11778A. Therefore, not only the common primary LHON mutation is responsible for the visual atrophy, but other secondary mtDNA mutations should also be considered when giving genetic counseling.展开更多
We studied the kinetic characterizations of the Trifolium pratense seedlings copper-containing amine oxidase(TPAO) by using various amine-containing substrates.The catalyzing rate for all of amine-containing substrate...We studied the kinetic characterizations of the Trifolium pratense seedlings copper-containing amine oxidase(TPAO) by using various amine-containing substrates.The catalyzing rate for all of amine-containing substrates can be ordered as diamines > polyamines > aromatic monoamines,and it shows an apparent trend in each category of substrates such as the longer the carbon chain,the lower the V_(max) is,so does the V_(max)/K_m values but is opposite for the K_m value of TPAO.The distinct differences between the kinetic parameters for different amine-containing substrates indicated that the rate-determining step of the catalytic reaction strongly depends on the substrate's chemical structure.It is concluded that both pH and ionic strength can affect the catalytic activity of TPAO via influencing the coulomb interaction-mediated enzyme-substrate docking processes,which can be attributed to the potential of charged groups from both substrates and the activity sites of TPAO by the regulation of ionic strength.展开更多
YVO4:Er^3+,Yb^3+ with varying Yb^3+ concentrations were prepared by a precipitation method.The results of X-ray diffraction (XRD) show that all the samples have a tetragonal zircon structure; the calculated aver...YVO4:Er^3+,Yb^3+ with varying Yb^3+ concentrations were prepared by a precipitation method.The results of X-ray diffraction (XRD) show that all the samples have a tetragonal zircon structure; the calculated average crystallite sizes are in the range of 14-22 nm.The lattice constants and cell volume of the samples decrease slightly with the increase in Yb^3+ concentration.The upconversion luminescence spectra of all the samples were studied under 980 nm laser excitation.The strong green emission is observed,which is attributed to the 2^H11/2→4I15/2 and 4^S3/2→4^I15/2 transitions of Er^3+,and the red emission peaks in 650-675 nm can be ignored.The emission intensity for the sample depends on the Yb^3+concentration.These results reveal that the upconversion processes of YVO4:Er^3+,Yb^3+ are related to the structure and the doping Yb^3+ concentration of the sample.展开更多
The morphology manipulation of the active layers is important for improving the performance of organic photovoltaics(OPVs).The choice of processing solvent has great impact on the crystallization and phase separation ...The morphology manipulation of the active layers is important for improving the performance of organic photovoltaics(OPVs).The choice of processing solvent has great impact on the crystallization and phase separation during film formation,since solvent properties,including solvent effect on molecular crystallization,boiling point,and interaction parameters,can directly change the evolution pathways associated with thermodynamics and kinetics.Therefore,revealing the underlying solvent-regulated morphology mechanism is potential to provide guiding strategies for device optimization.In this study,chloroform,chlorobenzene,and toluene are used to process PM6:Y6 blends by slot-die printing to fabricate OPV devices.The chloroform printed film forms a fibrillar network morphology with enhanced crystallization,facilitating exciton dissociation,charge transport and extraction,resulting in an optimal power conversion efficiency of 16.22%.However,the addition of the additive chloronaphthalene in chloroform solution leads to over-crystallization of Y6,and thus,increasing domain size that exceeds the exciton diffusion length,resulting in lower device efficiency.In addition,both the chlorobenzene and toluene suppress the crystallization of Y6,which drastically decreased short-circuit current and fill factor.These results demonstrate the important role of processing solvent in dictating film morphology,which critically connects with the resultant printed OPV performance.展开更多
Objective: To compare postoperative outcomes of full-bed deep anterior lamellar keratoplasty (DALK) with penetrating keratoplasty (PK) in treating keratoconus. Methods: Seventy-five eyes of 64 patients who received fu...Objective: To compare postoperative outcomes of full-bed deep anterior lamellar keratoplasty (DALK) with penetrating keratoplasty (PK) in treating keratoconus. Methods: Seventy-five eyes of 64 patients who received full-bed DALK and 52 eyes of 51 patients who received PK between June 2000 and August 2010 were included in this retrospective study. Full-bed DALK was performed using Yao's hooking-detaching technique. PK was performed using a standard technique. Intraoperative and postoperative complications, visual acuity, rejection, graft survival, endothelial cell density, corneal sensation recovery, and re-innervation were compared between the two groups. Results: A best correct visual acuity of 0.5 or better was achieved in 90.7% of eyes after full-bed DALK and in 92.3% of eyes after PK (P=0.75). By the fifth postoperative year, graft endothelial cell loss reached 34.6% in the PK group vs. 13.9% in the full-bed DALK group (P<0.001). There were no statistical differences in corneal sensitivity recovery or corneal re-innervation between the groups (P>0.05). Intraoperative microperforation occurred in seven out of 75 (9.3%) eyes with a temporally postoperative double anterior chamber in two eyes in the full-bed DALK group. Postoperative complications in the PK vs. the full-bed DALK groups respectively were: rejection (7.7% vs. 0%, P=0.015), high intraocular pressure (IOP) (46.2% vs. 1.3%, P<0.001), secondary glaucoma (9.6% vs. 0%, P=0.006), complicated cataract (19.2% vs. 0%, P<0.001), and wound dehiscence (9.6% vs. 0%, P=0.006). Conclusions: Both full-bed DALK and PK can offer long-term satisfactory visual outcomes for keratoconus. Graft rejection, secondary glaucoma, complicated cataracts, and constant endothelial cell loss were observed in eyes only after PK.展开更多
This review summarizes the recent progress of efficient room temperature phosphorescence(RTP) from pure organic luminogens achieved by crystallization-induced phosphorescence(CIP),with focus on the advances in our...This review summarizes the recent progress of efficient room temperature phosphorescence(RTP) from pure organic luminogens achieved by crystallization-induced phosphorescence(CIP),with focus on the advances in our group.Besides homocrystals,mixed crystals and cocrystals are also discussed.Meanwhile,intriguing RTP emission from the luminogens without conventional chromophores is demonstrated.展开更多
In this study,a new twisting gold(Ⅰ) isocyanide complex based on tetraphenylethene(TPE),TPE-NC-Au.was designed and synthesized.It exhibits aggregation induced phosphorescence(AIP) characteristics,owing to the i...In this study,a new twisting gold(Ⅰ) isocyanide complex based on tetraphenylethene(TPE),TPE-NC-Au.was designed and synthesized.It exhibits aggregation induced phosphorescence(AIP) characteristics,owing to the incorporation of Au moiety and conformation rigidification in the aggregated states.Moreover,the emission color of the crystalline solid of TPE-NC-Au changes from blue(454 nm) to green(500 nm) in response to mechanical grinding,due to the combined effects of conformation planarization,enhanced π…π stacking,as well as the emergence of aurophilic interactions in the ground amorphous state.Notably,the emission color can be restored upon solvent fuming,associating with the reconstruction of crystalline lattices.The AIP and switchable mechanochromism of TPE-NC-Au make it suitable for potential applications in bioimaging,sensing,and optoelectronic devices.展开更多
The manipulation of the morphology of the active layers is crucial for improving the performance of organic photovoltaic(OPV)devices. In particular, the development of non-fullerene acceptors(NFAs) has led to a large ...The manipulation of the morphology of the active layers is crucial for improving the performance of organic photovoltaic(OPV)devices. In particular, the development of non-fullerene acceptors(NFAs) has led to a large number of new materials with more complex interactions. Therefore, the investigation on the morphology control mechanism is the key aspect in providing guidance for material design and device optimization. In this study, the film morphology optimization using 1,8-diiodooctane(DIO) additive and a ternary fullerene acceptor strategy have been carried out based on the PCE10:ITIC blends. It is seen that suitable amount of DIO helps to increase the crystallization of the blended thin film. However, excessive DIO elevates the crystallization-induced phase separation and the domain size can exceed the exciton diffusion length, leading to efficiency drop. The addition of fullerene acceptor can improve the carrier transport of the blends, and its presence could retard the excessive phase separation induced by DIO additive. Under the joint optimization of the solvent additive and PCBM acceptor,the film morphology achieves a balance between crystallization and phase separation scales, the exciton diffusion and carrier transport are also optimized, and the short-circuit current(JSC) and fill factor(FF) of the device can be improved significantly.展开更多
基金Supported by the National Natural Science Foundation of China(No.J0710043)
文摘AIM: To investigate mitochondrial factors associated with Leber hereditary optic neuropathy (LHON) through complete sequencing and analysis of the mitochondrial genome of Chinese patients with this disease. METHODS: Two unrelated southern Chinese families with LHON and 10 matched healthy controls were recruited, and their entire mitochondrial DNA (mtDNA) was amplified and sequenced with the universal M13 primer. Then DNA sequence analysis and variation identification were performed by DNAssist and Chromas 2 software and compared with authoritative databases such as Mitomap. RESULTS: Mutational analysis of mtDNA in these two Chinese pedigrees revealed one common LHON-associated mutation, G11778A (Arg -> His), in the MT-ND4 gene. In addition, there were two secondary mutations in Pedigree 1: C34971 (Ala -> Val), and C3571T (Leu -> Phe) in the MT-ND1 gene, which have not been reported; and two secondary mutations occurred in Pedigree 2: A10398G (Thr -> Ala) in the MT-ND3 gene, and T14502C (Ile -> Val) in the MT-ND6 gene. Three polymorphisms, A73G, G94A and A263G in the mtDNA control region, were also found. CONCLUSION: Our study confirmed that the known MT-ND4* G11778A mutation is the most significant cause of LHON. The C3497T and C3571T mutations in Pedigree 1 were also both at hot-spots of MT-ND1; they may affect the respiratory chain in coordination with the primary mutation G11778A. In Pedigree 2, the two secondary mutations A10398G of MT-ND3 and T14502C of MT-ND6 may influence mitochondrial respiratory complex I, leading to the mitochondrial respiratory chain dysfunction which results in optic atrophy together with G11778A. Therefore, not only the common primary LHON mutation is responsible for the visual atrophy, but other secondary mtDNA mutations should also be considered when giving genetic counseling.
基金supported by the Department of Education of Inner Mongolia and Inner Mongolia Natural Science Foundation of China for Basic Research(No.NJZY13054)the National Natural Science Foundation of China(Nos.31360214,21171086 and 81160213)+3 种基金the Inner Mongolia Autonomous Region Science and Technology Department(No.211-202077)the Inner Mongolia Grassland Talent(No.108-108038)the Natural Science Foundation of Inner Mongolia Autonomous Region of China(Nos.2013MS1121 and 2015MS0806)the Inner Mongolia Agricultural University(Nos.109-108040,211-109003 and 211-206038)
文摘We studied the kinetic characterizations of the Trifolium pratense seedlings copper-containing amine oxidase(TPAO) by using various amine-containing substrates.The catalyzing rate for all of amine-containing substrates can be ordered as diamines > polyamines > aromatic monoamines,and it shows an apparent trend in each category of substrates such as the longer the carbon chain,the lower the V_(max) is,so does the V_(max)/K_m values but is opposite for the K_m value of TPAO.The distinct differences between the kinetic parameters for different amine-containing substrates indicated that the rate-determining step of the catalytic reaction strongly depends on the substrate's chemical structure.It is concluded that both pH and ionic strength can affect the catalytic activity of TPAO via influencing the coulomb interaction-mediated enzyme-substrate docking processes,which can be attributed to the potential of charged groups from both substrates and the activity sites of TPAO by the regulation of ionic strength.
基金supported by the Educational Department Project of Liaoning Province(No.2005319)
文摘YVO4:Er^3+,Yb^3+ with varying Yb^3+ concentrations were prepared by a precipitation method.The results of X-ray diffraction (XRD) show that all the samples have a tetragonal zircon structure; the calculated average crystallite sizes are in the range of 14-22 nm.The lattice constants and cell volume of the samples decrease slightly with the increase in Yb^3+ concentration.The upconversion luminescence spectra of all the samples were studied under 980 nm laser excitation.The strong green emission is observed,which is attributed to the 2^H11/2→4I15/2 and 4^S3/2→4^I15/2 transitions of Er^3+,and the red emission peaks in 650-675 nm can be ignored.The emission intensity for the sample depends on the Yb^3+concentration.These results reveal that the upconversion processes of YVO4:Er^3+,Yb^3+ are related to the structure and the doping Yb^3+ concentration of the sample.
基金financially supported by the National Natural Science Foundation of China(Nos.51973110,21734009,21905102 and 22109094)the Program of Shanghai Science and Technology Commission science and technology innovation action plan(Nos.20ZR1426200,20511103800,20511103802 and 20511103803)+2 种基金the Natural Science Foundation of Shandong Province(No.ZR2019LFG005)the Key research project of Shandong Province(No.2020CXGC010403)the Center of Hydrogen Science,Shanghai Jiao Tong University,China。
文摘The morphology manipulation of the active layers is important for improving the performance of organic photovoltaics(OPVs).The choice of processing solvent has great impact on the crystallization and phase separation during film formation,since solvent properties,including solvent effect on molecular crystallization,boiling point,and interaction parameters,can directly change the evolution pathways associated with thermodynamics and kinetics.Therefore,revealing the underlying solvent-regulated morphology mechanism is potential to provide guiding strategies for device optimization.In this study,chloroform,chlorobenzene,and toluene are used to process PM6:Y6 blends by slot-die printing to fabricate OPV devices.The chloroform printed film forms a fibrillar network morphology with enhanced crystallization,facilitating exciton dissociation,charge transport and extraction,resulting in an optimal power conversion efficiency of 16.22%.However,the addition of the additive chloronaphthalene in chloroform solution leads to over-crystallization of Y6,and thus,increasing domain size that exceeds the exciton diffusion length,resulting in lower device efficiency.In addition,both the chlorobenzene and toluene suppress the crystallization of Y6,which drastically decreased short-circuit current and fill factor.These results demonstrate the important role of processing solvent in dictating film morphology,which critically connects with the resultant printed OPV performance.
基金Project (No. 2011C13029-2) supported by the Major Program for Science and Technology Research of Zhejiang Province, China
文摘Objective: To compare postoperative outcomes of full-bed deep anterior lamellar keratoplasty (DALK) with penetrating keratoplasty (PK) in treating keratoconus. Methods: Seventy-five eyes of 64 patients who received full-bed DALK and 52 eyes of 51 patients who received PK between June 2000 and August 2010 were included in this retrospective study. Full-bed DALK was performed using Yao's hooking-detaching technique. PK was performed using a standard technique. Intraoperative and postoperative complications, visual acuity, rejection, graft survival, endothelial cell density, corneal sensation recovery, and re-innervation were compared between the two groups. Results: A best correct visual acuity of 0.5 or better was achieved in 90.7% of eyes after full-bed DALK and in 92.3% of eyes after PK (P=0.75). By the fifth postoperative year, graft endothelial cell loss reached 34.6% in the PK group vs. 13.9% in the full-bed DALK group (P<0.001). There were no statistical differences in corneal sensitivity recovery or corneal re-innervation between the groups (P>0.05). Intraoperative microperforation occurred in seven out of 75 (9.3%) eyes with a temporally postoperative double anterior chamber in two eyes in the full-bed DALK group. Postoperative complications in the PK vs. the full-bed DALK groups respectively were: rejection (7.7% vs. 0%, P=0.015), high intraocular pressure (IOP) (46.2% vs. 1.3%, P<0.001), secondary glaucoma (9.6% vs. 0%, P=0.006), complicated cataract (19.2% vs. 0%, P<0.001), and wound dehiscence (9.6% vs. 0%, P=0.006). Conclusions: Both full-bed DALK and PK can offer long-term satisfactory visual outcomes for keratoconus. Graft rejection, secondary glaucoma, complicated cataracts, and constant endothelial cell loss were observed in eyes only after PK.
基金financially supported by the National Natural Science Foundation of China(No.51473092)the Shanghai Rising-Star Program(No.15QA1402500)the SMC-Chenxing Young Scholar Program of Shanghai Jiao Tong University
文摘This review summarizes the recent progress of efficient room temperature phosphorescence(RTP) from pure organic luminogens achieved by crystallization-induced phosphorescence(CIP),with focus on the advances in our group.Besides homocrystals,mixed crystals and cocrystals are also discussed.Meanwhile,intriguing RTP emission from the luminogens without conventional chromophores is demonstrated.
基金financially supported by the National Natural Science Foundation of China(No.51473092)the Shanghai Rising-Star Program(No.15QA1402500)
文摘In this study,a new twisting gold(Ⅰ) isocyanide complex based on tetraphenylethene(TPE),TPE-NC-Au.was designed and synthesized.It exhibits aggregation induced phosphorescence(AIP) characteristics,owing to the incorporation of Au moiety and conformation rigidification in the aggregated states.Moreover,the emission color of the crystalline solid of TPE-NC-Au changes from blue(454 nm) to green(500 nm) in response to mechanical grinding,due to the combined effects of conformation planarization,enhanced π…π stacking,as well as the emergence of aurophilic interactions in the ground amorphous state.Notably,the emission color can be restored upon solvent fuming,associating with the reconstruction of crystalline lattices.The AIP and switchable mechanochromism of TPE-NC-Au make it suitable for potential applications in bioimaging,sensing,and optoelectronic devices.
基金financially supported by the National Natural Science Foundation of China (Nos.51973110,21734009,21905102 and 22109094)the National Key R&D Program of China (Nos.2020YFB1505500 and 2020YFB1505502)+3 种基金the Program of Shanghai Science and Technology Commission science and technology innovation action plan (Nos.20ZR1426200,20511103800,20511103802 and 20511103803)the Natural Science Foundation of Shandong Province (No.ZR2019LFG005)the Key research project of Shandong Province (No.2020CXGC010403)the Center of Hydrogen Science,Shanghai Jiao Tong University,China。
文摘The manipulation of the morphology of the active layers is crucial for improving the performance of organic photovoltaic(OPV)devices. In particular, the development of non-fullerene acceptors(NFAs) has led to a large number of new materials with more complex interactions. Therefore, the investigation on the morphology control mechanism is the key aspect in providing guidance for material design and device optimization. In this study, the film morphology optimization using 1,8-diiodooctane(DIO) additive and a ternary fullerene acceptor strategy have been carried out based on the PCE10:ITIC blends. It is seen that suitable amount of DIO helps to increase the crystallization of the blended thin film. However, excessive DIO elevates the crystallization-induced phase separation and the domain size can exceed the exciton diffusion length, leading to efficiency drop. The addition of fullerene acceptor can improve the carrier transport of the blends, and its presence could retard the excessive phase separation induced by DIO additive. Under the joint optimization of the solvent additive and PCBM acceptor,the film morphology achieves a balance between crystallization and phase separation scales, the exciton diffusion and carrier transport are also optimized, and the short-circuit current(JSC) and fill factor(FF) of the device can be improved significantly.