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Advancing One Health education:integrative pedagogical approaches and their impacts on interdisciplinary learning
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作者 Chang Cai yong-sam jung +6 位作者 Richard Van Vleck Pereira Michael S.M.Brouwer Junxia Song Bennie Irve Osburn Joanna McKenzie Wim H.M.van der Poel Yingjuan Qian 《Science in One Health》 2023年第1期90-94,共5页
One Health is an integrative approach that emphasizes the interconnectedness of human,animal,and environmental health,advocating for collaborative,multidisciplinary efforts to address health challenges,particularly am... One Health is an integrative approach that emphasizes the interconnectedness of human,animal,and environmental health,advocating for collaborative,multidisciplinary efforts to address health challenges,particularly amid globalization and emerging threats.This paper examines the integration of One Health principles into global health education,highlighting the importance of interdisciplinary collaboration and innovative pedagogical approaches.It evaluates various teaching methods,including problem-based learning(PBL),team-based learning(TBL),simulation-based education(SBE),case-based learning(CBL),interdisciplinary workshops and seminars(IWS),and service-learning(SL),analyzing their strengths and weaknesses in fostering interdisciplinary understanding and practical application of One Health concepts.While these methods enhance learning by promoting critical thinking,collaboration,and real-world application,they also face challenges such as resource constraints,variability in group dynamics,and the complexity of assessing long-term learning outcomes.The paper also discusses the role of global partnerships,such as the Global One Health Research Partnership(GOHRP),in advancing One Health education through collaborative research and educational initiatives.Addressing challenges in curriculum integration and interdisciplinary collaboration is crucial for the effective implementation of One Health education,ensuring that future health professionals are equipped to tackle complex global health challenges. 展开更多
关键词 One Health education Interdisciplinary collaboration Global health integration Pedagogical approaches Curriculum integration Global partnerships
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非洲猪瘟病毒外膜蛋白CD2v的原核表达及其免疫特性的研究 被引量:7
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作者 钟秋萍 于婉琪 +8 位作者 薄宗义 吕璐 谢振华 王振忠 郑龙三 张志芳 扈荣良 钱莺娟 陈鸿军 《中国兽医科学》 CAS CSCD 北大核心 2020年第6期689-695,共7页
本研究参考非洲猪瘟病毒(ASFV)国内分离株SY18株的EP402R基因(编码CD2v蛋白),优化其序列,设计特异性引物扩增。将去除跨膜保留胞外区或胞内区的两种截短体克隆到原核表达载体p ET-28a中,再转化至大肠杆菌BL21(DE3)感受态细胞。经IPTG诱... 本研究参考非洲猪瘟病毒(ASFV)国内分离株SY18株的EP402R基因(编码CD2v蛋白),优化其序列,设计特异性引物扩增。将去除跨膜保留胞外区或胞内区的两种截短体克隆到原核表达载体p ET-28a中,再转化至大肠杆菌BL21(DE3)感受态细胞。经IPTG诱导,铁蛋白融合CD2v胞外区(CD2vN-Fe)和铁蛋白融合CD2v胞外-胞内区(CD2v-NC-Fe)均获得高效表达。以该融合蛋白为抗原免疫BALB/c小鼠制备多克隆抗体,通过酶联免疫吸附实验(ELISA)和间接免疫荧光(IFA)方法检测其灵敏度和特异性。结果表明:CD2vN-Fe多抗特异性较强。本研究为建立ASFV的血清学鉴别检测方法奠定了基础。 展开更多
关键词 非洲猪瘟病毒 CD2v 原核表达 抗体
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