During the transient process of gas drilling conditions,the monitoring data often has obvious nonlinear fluctuation features,which leads to large classification errors and time delays in the commonly used intelligent ...During the transient process of gas drilling conditions,the monitoring data often has obvious nonlinear fluctuation features,which leads to large classification errors and time delays in the commonly used intelligent classification models.Combined with the structural features of data samples obtained from monitoring while drilling,this paper uses convolution algorithm to extract the correlation features of multiple monitoring while drilling parameters changing with time,and applies RBF network with nonlinear classification ability to classify the features.In the training process,the loss function component based on distance mean square error is used to effectively adjust the best clustering center in RBF.Many field applications show that,the recognition accuracy of the above nonlinear classification network model for gas production,water production and drill sticking is 97.32%,95.25%and 93.78%.Compared with the traditional convolutional neural network(CNN)model,the network structure not only improves the classification accuracy of conditions in the transition stage of conditions,but also greatly advances the time points of risk identification,especially for the three common risk identification points of gas production,water production and drill sticking,which are advanced by 56,16 and 8 s.It has won valuable time for the site to take correct risk disposal measures in time,and fully demonstrated the applicability of nonlinear classification neural network in oil and gas field exploration and development.展开更多
Nicotiflorin is a flavonoid extracted from Carthamus tinctorius.Previous studies have shown its cerebral protective effect,but the mechanism is undefined.In this study,we aimed to determine whether nicotiflorin protec...Nicotiflorin is a flavonoid extracted from Carthamus tinctorius.Previous studies have shown its cerebral protective effect,but the mechanism is undefined.In this study,we aimed to determine whether nicotiflorin protects against cerebral ischemia/reperfusion injury-induced apoptosis through the JAK2/STAT3 pathway.The cerebral ischemia/reperfusion injury model was established by middle cerebral artery occlusion/reperfusion.Nicotiflorin(10 mg/kg) was administered by tail vein injection.Cell apoptosis in the ischemic cerebral cortex was examined by hematoxylin-eosin staining and terminal deoxynucleotidyl transferase d UTP nick end labeling assay.Bcl-2 and Bax expression levels in ischemic cerebral cortex were examined by immunohistochemial staining.Additionally,p-JAK2,p-STAT3,Bcl-2,Bax,and caspase-3 levels in ischemic cerebral cortex were examined by western blot assay.Nicotiflorin altered the shape and structure of injured neurons,decreased the number of apoptotic cells,down-regulates expression of p-JAK2,p-STAT3,caspase-3,and Bax,decreased Bax immunoredactivity,and increased Bcl-2 protein expression and immunoreactivity.These results suggest that nicotiflorin protects against cerebral ischemia/reperfusion injury-induced apoptosis via the JAK2/STAT3 pathway.展开更多
Objective To investigate the frequency of GAG deletion in the DYT1 gene among early onset primary dystonia patients in China.Methods Thirteen patients with early onset primary torsion dystonia were screened for mutati...Objective To investigate the frequency of GAG deletion in the DYT1 gene among early onset primary dystonia patients in China.Methods Thirteen patients with early onset primary torsion dystonia were screened for mutation in exon 5 of the DYT1 gene using denaturing high-performance liquid chromatography(DHPLC)and DNA sequencing,and the results were confirmed with polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results The GAG deletion mutation which results in Glu302del in exon 5 of the DYT1 gene was found in 5 patients.The detecting results were consistent between with DHPLC and PCR-RFLP.We did not find any other mutations in the DYT1 gene.Conclusions The GAG deletion in the DYT1 gene is common amongst early onset primary torsion dystonia patients in China.The frequency of DYT1 mutation is not significantly different between European and Asian patients with early onset primary dystonia.展开更多
We propose an efficient method of generating a vortex beam with multi-foci by using a fractal spiral zone plate(FSZP), which is designed by combining fractal structure with a spiral zone plate(SZP) in the squared radi...We propose an efficient method of generating a vortex beam with multi-foci by using a fractal spiral zone plate(FSZP), which is designed by combining fractal structure with a spiral zone plate(SZP) in the squared radial coordinate.The theoretical analysis reveals that the number of foci that embed vortices is significantly increased as compared with that obtained by using a conventional SZP. Furthermore, the influence of topological charge on the intensity distribution in focal plane is also discussed in detail. For experimental investigation, an FSZP with topological charge p = 1 and 6.4 mm diameter is fabricated by using a photo-etching technique. The calibration indicates that the focusing performances of such a kind of zone plane(ZP) accord well with simulations, thereby providing its potential applications in multi-dimensional optical manipulation and optical imaging technology.展开更多
Brief-pulse stimulation at 50 Hz has been shown to terminate afterdischarges observed in epilepsy patients. However, the optimal pulse stimulation parameters for terminating cortical electrical stimulation-induced aft...Brief-pulse stimulation at 50 Hz has been shown to terminate afterdischarges observed in epilepsy patients. However, the optimal pulse stimulation parameters for terminating cortical electrical stimulation-induced afterdischarges remain unclear. In the present study, we examined the effects of different brief-pulse stimulation frequencies(5, 50 and 100 Hz) on cortical electrical stimulation-induced afterdischarges in 10 patients with refractory epilepsy. Results demonstrated that brief-pulse stimulation could terminate cortical electrical stimulation-induced afterdischarges in refractory epilepsy patients. In conclusion,(1) a brief-pulse stimulation was more effective when the afterdischarge did not extend to the surrounding brain area.(2) A higher brief-pulse stimulation frequency(especially 100 Hz) was more likely to terminate an afterdischarge.(3) A low current intensity of brief-pulse stimulation was more likely to terminate an afterdischarge.展开更多
Background: Deep brain stimulation (DBS) has been a promising treatment for patients with refractory Tourette syndrome (TS) for more than a decade. Despite successful DBS treatment of TS in more than 100 patients...Background: Deep brain stimulation (DBS) has been a promising treatment for patients with refractory Tourette syndrome (TS) for more than a decade. Despite successful DBS treatment of TS in more than 100 patients worldwide, studies with a large patient sample and long-term follow-up assessments are still scarce. Accordingly, we investigated the clinical efficacy and safety of globus pallidus internus (GPi) DBS in the treatment of intractable TS in 24 patients with a 1-year follow-up assessment. Methods: Bilateral/unilateral GPi-DBS was performed in 24 patients with TS. We evaluated symptoms of tics and obsessive-compulsive disorder (OCD) through the Yale Global Tic Severity Scale (YGTSS) and Yale-Brown Obsessive-compulsive Scale (Y-BOCS). We used the Wechsler Adult Intelligence Scale-Revised in China (WAIS-RC) to evaluate the safety of the treatment. We conducted follow-up assessments of all patients for at least 12 months (12-99 months). Results: Symptoms of tics and OCD were significantly relieved at a 12-month follow-up assessment. The mean YGTSS score was 74.04 ± 11.52, 49.83 ± 10.91, 32.58 ± 7.97, and 31.21 ± 8.87 at baseline, 3, 6, and 12 months, respectively. The mean YGTSS scores obtained at the follow-up assessments were significantly different from the baseline (P 〈 0.05). The improvement in motor tics was superior to that in phonic tics. The mean Y-BOCS scores were 21.61 ± 4.97, 18 ± 4.58, 14.39 ± 3.99, and 13.78 ± 4.56 at baseline, 3, 6, and 12 months, respectively (P 〈 0.05). We observed a remarkable improvement in psychiatric comorbidities, such as OCD and attention-deficit hyperactivity disorder, after the procedure. WAIS-RC scores were comparable before and after the operation. There were no severe postoperative complications. Conclusion: GPi-DBS appears to comprehensively alleviate tic symptoms and psychiatric comorbidities in patients with TS, thus significantly improving patients' quality of life.展开更多
Background:Dysembryoplastic neuroepithelial tumors(DNTs)are common causes of intractable epilepsy in pediatric epilepsy patients.The effect of surgical intervention is often limited when the tumor is located in the pr...Background:Dysembryoplastic neuroepithelial tumors(DNTs)are common causes of intractable epilepsy in pediatric epilepsy patients.The effect of surgical intervention is often limited when the tumor is located in the precentral gyrus.Furthermore,complete surgical resection is often not performed in order to avoid permanent neurological deficits.Methods:Here,we present a pediatric patient with intractable epilepsy caused by a simple DNT located in the precentral gyrus.Intracranial electrodes were implanted and used in combination with magnetic resonance imaging,video-electroencephalography and electrical cortical stimulation to assess neurological function,and where the epileptogenic zone was located.Results:The results of intracranial electrode monitoring suggested that the epileptogenic zone was located in the tumor area and that cortical function had been reorganized.We completely resected the tumor based on these findings.The patient has been seizure free after the surgery and has not had any neurological deficits.Conclusions:Simple form DNTs in the precentral gyrus can be completely resected with careful preoperative assessment of cortical function.Cortical reorganization could partly explain the functional preservation after surgery.展开更多
Background:Patients with temporal lobe epilepsy(TLE)originating from different seizure onset zones had distinct electrophysiological characteristics and surgical outcomes.In this study,we aimed to investigate the rela...Background:Patients with temporal lobe epilepsy(TLE)originating from different seizure onset zones had distinct electrophysiological characteristics and surgical outcomes.In this study,we aimed to investigate the relationship between the origin and prognosis of TLE,and the stereoelectroencephalography(SEEG)features.Methods:Thirty patients with TLE,who underwent surgical treatment in our functional neurosurgery department from January 2016 to December 2017,were enrolled in this study.All patients underwent anterior temporal lobectomy after an invasive preoperative evaluation with SEEG.Depending on the epileptic focus location,patients were divided into those with medial temporal lobe seizures(MTLS)and those with lateral temporal lobe seizures(LTLS).The Engel classification was used to evaluate operation effectiveness,and the Kaplan-Meier analysis was used to detect seizure-free duration.Results:The mean follow-up time was 25.7±4.8 months.Effectiveness was 63.3%for Engel I(n=19),13.3%for Engel II,3.3%for Engel III,and 20.0%for Engel IV.According to the SEEG,60.0%(n=18)had MTLS,and 40.0%(n=12)had LTLS.Compared with the MTLS group,the operation age of those with LTLS was significantly greater(26.9±6.9 vs.29.9±12.5 years,t=-0.840,P=0.009)with longer epilepsy duration(11.9±6.0 vs.17.9±12.1 years,t=-1.801,P=0.038).Patients with MTLS had a longer time interval between ictal onset to seizure(67.3±59.1 s vs.29.3±24.4 s,t=2.017,P=0.008).The most common SEEG ictal pattern was a sharp/spike-wave rhythm in the MTLS group(55.6%)and low-voltage fast activity in the LTLS group(58.3%).Compared with the LTLS group,patients with MTLS had a more favorable prognosis(41.7%vs.77.8%,P=0.049).Post-operative recurrence was more likely to occur within three months after the operation for both groups,and there appeared to be a stable longterm outcome.Conclusion:Patients with MTLS,who accounted for three-fifths of patients with TLE,showed a more favorable surgical outcome.展开更多
基金supported by the National Key R&D Program of China(2019YFA0708303)the Sichuan Science and Technology Program(2021YFG0318)+2 种基金the Engineering Technology Joint Research Institute Project of CCDC-SWPU(CQXN-2021-03)the PetroChina Innovation Foundation(2020D-5007-0312)the Key projects of NSFC(61731016).
文摘During the transient process of gas drilling conditions,the monitoring data often has obvious nonlinear fluctuation features,which leads to large classification errors and time delays in the commonly used intelligent classification models.Combined with the structural features of data samples obtained from monitoring while drilling,this paper uses convolution algorithm to extract the correlation features of multiple monitoring while drilling parameters changing with time,and applies RBF network with nonlinear classification ability to classify the features.In the training process,the loss function component based on distance mean square error is used to effectively adjust the best clustering center in RBF.Many field applications show that,the recognition accuracy of the above nonlinear classification network model for gas production,water production and drill sticking is 97.32%,95.25%and 93.78%.Compared with the traditional convolutional neural network(CNN)model,the network structure not only improves the classification accuracy of conditions in the transition stage of conditions,but also greatly advances the time points of risk identification,especially for the three common risk identification points of gas production,water production and drill sticking,which are advanced by 56,16 and 8 s.It has won valuable time for the site to take correct risk disposal measures in time,and fully demonstrated the applicability of nonlinear classification neural network in oil and gas field exploration and development.
基金financially supported by the Natural Science Foundation of Education Department of Sichuan Province of China,No.14ZB0152the Joint Research Program of Luzhou and Southwest Medical University,in China,No.14JC0120
文摘Nicotiflorin is a flavonoid extracted from Carthamus tinctorius.Previous studies have shown its cerebral protective effect,but the mechanism is undefined.In this study,we aimed to determine whether nicotiflorin protects against cerebral ischemia/reperfusion injury-induced apoptosis through the JAK2/STAT3 pathway.The cerebral ischemia/reperfusion injury model was established by middle cerebral artery occlusion/reperfusion.Nicotiflorin(10 mg/kg) was administered by tail vein injection.Cell apoptosis in the ischemic cerebral cortex was examined by hematoxylin-eosin staining and terminal deoxynucleotidyl transferase d UTP nick end labeling assay.Bcl-2 and Bax expression levels in ischemic cerebral cortex were examined by immunohistochemial staining.Additionally,p-JAK2,p-STAT3,Bcl-2,Bax,and caspase-3 levels in ischemic cerebral cortex were examined by western blot assay.Nicotiflorin altered the shape and structure of injured neurons,decreased the number of apoptotic cells,down-regulates expression of p-JAK2,p-STAT3,caspase-3,and Bax,decreased Bax immunoredactivity,and increased Bcl-2 protein expression and immunoreactivity.These results suggest that nicotiflorin protects against cerebral ischemia/reperfusion injury-induced apoptosis via the JAK2/STAT3 pathway.
基金Supported by grants from Ministry of Sciences and Technology of China(2006CB500701,2002BA711A10)National Natural Science Foundation of China(30430280)+1 种基金Beijing Municipal Commission on Sciences and Technology(H020220020610,7031002)Beijing Bureau of Health(2003-2029)
文摘Objective To investigate the frequency of GAG deletion in the DYT1 gene among early onset primary dystonia patients in China.Methods Thirteen patients with early onset primary torsion dystonia were screened for mutation in exon 5 of the DYT1 gene using denaturing high-performance liquid chromatography(DHPLC)and DNA sequencing,and the results were confirmed with polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results The GAG deletion mutation which results in Glu302del in exon 5 of the DYT1 gene was found in 5 patients.The detecting results were consistent between with DHPLC and PCR-RFLP.We did not find any other mutations in the DYT1 gene.Conclusions The GAG deletion in the DYT1 gene is common amongst early onset primary torsion dystonia patients in China.The frequency of DYT1 mutation is not significantly different between European and Asian patients with early onset primary dystonia.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11404290 and 61505178)the Major National Scientific Instruments Developing Special Project(Grant No.2012YQ130125)+2 种基金the Postdoctoral Science Foundation of Henan Province,China(Grant No.2013008)the Program for Science&Technology Innovation Talents in Universities of Henan Province,China(Grant No.172102210481)the Key Scientific Research Projects in Universities of Henan Province,China(Grant No.19B140005)
文摘We propose an efficient method of generating a vortex beam with multi-foci by using a fractal spiral zone plate(FSZP), which is designed by combining fractal structure with a spiral zone plate(SZP) in the squared radial coordinate.The theoretical analysis reveals that the number of foci that embed vortices is significantly increased as compared with that obtained by using a conventional SZP. Furthermore, the influence of topological charge on the intensity distribution in focal plane is also discussed in detail. For experimental investigation, an FSZP with topological charge p = 1 and 6.4 mm diameter is fabricated by using a photo-etching technique. The calibration indicates that the focusing performances of such a kind of zone plane(ZP) accord well with simulations, thereby providing its potential applications in multi-dimensional optical manipulation and optical imaging technology.
基金supported by the Capital Health Research and Development Special Funds of China,No.2016-1-2011
文摘Brief-pulse stimulation at 50 Hz has been shown to terminate afterdischarges observed in epilepsy patients. However, the optimal pulse stimulation parameters for terminating cortical electrical stimulation-induced afterdischarges remain unclear. In the present study, we examined the effects of different brief-pulse stimulation frequencies(5, 50 and 100 Hz) on cortical electrical stimulation-induced afterdischarges in 10 patients with refractory epilepsy. Results demonstrated that brief-pulse stimulation could terminate cortical electrical stimulation-induced afterdischarges in refractory epilepsy patients. In conclusion,(1) a brief-pulse stimulation was more effective when the afterdischarge did not extend to the surrounding brain area.(2) A higher brief-pulse stimulation frequency(especially 100 Hz) was more likely to terminate an afterdischarge.(3) A low current intensity of brief-pulse stimulation was more likely to terminate an afterdischarge.
基金This project was supported by a grant from National Natural Science Foundation of China (No. 81371256).
文摘Background: Deep brain stimulation (DBS) has been a promising treatment for patients with refractory Tourette syndrome (TS) for more than a decade. Despite successful DBS treatment of TS in more than 100 patients worldwide, studies with a large patient sample and long-term follow-up assessments are still scarce. Accordingly, we investigated the clinical efficacy and safety of globus pallidus internus (GPi) DBS in the treatment of intractable TS in 24 patients with a 1-year follow-up assessment. Methods: Bilateral/unilateral GPi-DBS was performed in 24 patients with TS. We evaluated symptoms of tics and obsessive-compulsive disorder (OCD) through the Yale Global Tic Severity Scale (YGTSS) and Yale-Brown Obsessive-compulsive Scale (Y-BOCS). We used the Wechsler Adult Intelligence Scale-Revised in China (WAIS-RC) to evaluate the safety of the treatment. We conducted follow-up assessments of all patients for at least 12 months (12-99 months). Results: Symptoms of tics and OCD were significantly relieved at a 12-month follow-up assessment. The mean YGTSS score was 74.04 ± 11.52, 49.83 ± 10.91, 32.58 ± 7.97, and 31.21 ± 8.87 at baseline, 3, 6, and 12 months, respectively. The mean YGTSS scores obtained at the follow-up assessments were significantly different from the baseline (P 〈 0.05). The improvement in motor tics was superior to that in phonic tics. The mean Y-BOCS scores were 21.61 ± 4.97, 18 ± 4.58, 14.39 ± 3.99, and 13.78 ± 4.56 at baseline, 3, 6, and 12 months, respectively (P 〈 0.05). We observed a remarkable improvement in psychiatric comorbidities, such as OCD and attention-deficit hyperactivity disorder, after the procedure. WAIS-RC scores were comparable before and after the operation. There were no severe postoperative complications. Conclusion: GPi-DBS appears to comprehensively alleviate tic symptoms and psychiatric comorbidities in patients with TS, thus significantly improving patients' quality of life.
文摘Background:Dysembryoplastic neuroepithelial tumors(DNTs)are common causes of intractable epilepsy in pediatric epilepsy patients.The effect of surgical intervention is often limited when the tumor is located in the precentral gyrus.Furthermore,complete surgical resection is often not performed in order to avoid permanent neurological deficits.Methods:Here,we present a pediatric patient with intractable epilepsy caused by a simple DNT located in the precentral gyrus.Intracranial electrodes were implanted and used in combination with magnetic resonance imaging,video-electroencephalography and electrical cortical stimulation to assess neurological function,and where the epileptogenic zone was located.Results:The results of intracranial electrode monitoring suggested that the epileptogenic zone was located in the tumor area and that cortical function had been reorganized.We completely resected the tumor based on these findings.The patient has been seizure free after the surgery and has not had any neurological deficits.Conclusions:Simple form DNTs in the precentral gyrus can be completely resected with careful preoperative assessment of cortical function.Cortical reorganization could partly explain the functional preservation after surgery.
基金supported by grants from the Beijing Natural Science Foundation(No.L182015)the Beijing Hospitals Authority’s Ascent Plan(No.DFL20190801)。
文摘Background:Patients with temporal lobe epilepsy(TLE)originating from different seizure onset zones had distinct electrophysiological characteristics and surgical outcomes.In this study,we aimed to investigate the relationship between the origin and prognosis of TLE,and the stereoelectroencephalography(SEEG)features.Methods:Thirty patients with TLE,who underwent surgical treatment in our functional neurosurgery department from January 2016 to December 2017,were enrolled in this study.All patients underwent anterior temporal lobectomy after an invasive preoperative evaluation with SEEG.Depending on the epileptic focus location,patients were divided into those with medial temporal lobe seizures(MTLS)and those with lateral temporal lobe seizures(LTLS).The Engel classification was used to evaluate operation effectiveness,and the Kaplan-Meier analysis was used to detect seizure-free duration.Results:The mean follow-up time was 25.7±4.8 months.Effectiveness was 63.3%for Engel I(n=19),13.3%for Engel II,3.3%for Engel III,and 20.0%for Engel IV.According to the SEEG,60.0%(n=18)had MTLS,and 40.0%(n=12)had LTLS.Compared with the MTLS group,the operation age of those with LTLS was significantly greater(26.9±6.9 vs.29.9±12.5 years,t=-0.840,P=0.009)with longer epilepsy duration(11.9±6.0 vs.17.9±12.1 years,t=-1.801,P=0.038).Patients with MTLS had a longer time interval between ictal onset to seizure(67.3±59.1 s vs.29.3±24.4 s,t=2.017,P=0.008).The most common SEEG ictal pattern was a sharp/spike-wave rhythm in the MTLS group(55.6%)and low-voltage fast activity in the LTLS group(58.3%).Compared with the LTLS group,patients with MTLS had a more favorable prognosis(41.7%vs.77.8%,P=0.049).Post-operative recurrence was more likely to occur within three months after the operation for both groups,and there appeared to be a stable longterm outcome.Conclusion:Patients with MTLS,who accounted for three-fifths of patients with TLE,showed a more favorable surgical outcome.