Donor-Derived Transmission of Scedosporiosis in Kidney Transplant Recipients from a Systemic lupus erythematosus donor

Donor-derived infection(DDI)associated with Scedosporium spp is extremely rare,and results in a very poor prognosis.The present study reports a probable DDI due to Scedosporium boydii(S.boydii)from a donor with neuropsychiatric systemic lupus erythematosus.Two recipients developed Scedosporiosis after kidney transplantation from the same donor.Recipient 1 died of central nervous system infection due to S.boydii based on the clinical presentations,and the positive metagenomic next-generation sequencing(mNGS)and culture results for the cerebrospinal fluid.The other recipient with urinary tract obstruction due to S.boydii,which was identified through the positive culture and mNGS results of the removed stents,was successfully treated by stent replacement and voriconazole administration.Undiagnosed disseminated donor infection and the transmission of S.boydii should be given attention,particularly when the donor and recipients have primary immunodeficiency disease.The screening of donors and recipients for S.boydii using mNGS may be helpful in guiding antifungal prophylaxis and treatment recipients,due to its higher sensitivity and shorter diagnostic time relative to other traditional techniques.

Simulation of plasma properties in magnetron sputtering for two kinds of cathode targets

Introduction During magnetron sputtering process,the common structure of cathode target is planar target and cylindrical rotating target.In this study,cylindrical rotating target is used and two kinds of cathode targets were investigated by COMSOL Multiphysics software(The official network of COMSOL Multiphysics software.https://uk.comsol.com/).We will elucidate the difference between the two types of cathode target and determine the type of cathode target used in the final experiment.The system configuration We explore the plasma distribution in the radio frequency cavity,so the simulation process was divided into two steps:building RF cavity model and setting up plasma discharge parameters.The main part of the model included the radio frequency cavity substrate(divided into two tube parts and middle ellipsoid part),the cathode and the magnet.And the plasma discharge parameters are as follows:Ar gas was used with 1.5 Pa;magnetic field strength of iron core was set to 1000 Gs;the applied voltage of cathode was set to-160 V;and anode was set to 0 V.Conclusion For the long cathode target and the short cathode target,the main difference is the electric field distribution.Because the electric field lines are denser for the long cathode target,the electric field intensity is stronger,and then the initial energy obtained by electrons is higher.During the plasma discharge process,because of the high electron energy,the plasma density produced is more than the simulation of the short cathode target.And under the same simulation time,the residual energy of electrons is more for the long cathode target,which is the reason for the higher electron temperature.From the previous experimental experience,we know that the film quality formed by higher electron energy is better.The simulation in this work shows that the electron energy corresponding to the long cathode target is higher than that of the short cathode target,so we choose the long cathode target as the experimental target in the subsequent coating experiments.

HMGR, SQS, β-AS, and Cytochrome P450 Monooxygenase Genes in Glycyrrhiza uralensis

Glycyrrhiza uralensis is frequently used in traditional Chinese medicine. This plant contains a large amount of effective constituents, including triterpenoids and flavonoids. Among them, glycyrrhizin is believed to be the marker compound to evaluate the quality of G. uralensis based on Chinese Pharmacopoeia. Many studies showed that glycyrrhizin possesses various pharmacological activities, such as antibacterial, antiviral, antitumor, anti-inflammatory, and immune-stimulating activities. In this paper, we summarized the cloning, characterization, expression, and polymorphism analysis of several functional genes involved in glycyrrhizin biosynthesis in G. uralensis.