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新型核素分子探针^(18)F-NT靶向前列腺癌的实验研究
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作者 邹开力 唐永祥 +2 位作者 周明 李建 胡硕 《中国现代医学杂志》 CAS 2018年第16期17-21,共5页
目的探讨新型核素分子探针^(18)F-NT在前列腺癌细胞及荷瘤小鼠的靶向性,为后续^(18)F-NT靶向前列腺癌的在体显像提供实验依据。方法制备^(18)F-NT,并完成质量控制检测。选取高表达神经降压素受体1(NTR1)的人前列腺癌细胞系PC3,分3组进... 目的探讨新型核素分子探针^(18)F-NT在前列腺癌细胞及荷瘤小鼠的靶向性,为后续^(18)F-NT靶向前列腺癌的在体显像提供实验依据。方法制备^(18)F-NT,并完成质量控制检测。选取高表达神经降压素受体1(NTR1)的人前列腺癌细胞系PC3,分3组进行细胞结合实验,分别为实验组、阻断组和对照组(游离^(18)F离子)。对照组在细胞中加入游离^(18)F离子,实验组在细胞中加^(18)F-NT,阻断组细胞中加入^(18)F-NT前30 min加入神经降压素(NT)进行阻断。复制荷PC3前列腺癌裸鼠模型,分为实验组和阻断组,每组3只。阻断组单次尾静脉注射NT 0.2 ml(浓度为1 mg/ml);实验组注射0.2 ml生理盐水,30 min后两组裸鼠尾静脉分别注射37 MBq/ml的^(18)F-NT 0.2 ml,1 h后处死裸鼠,分离主要脏器及肿瘤组织,γ计数器测量各脏器组织的放射性计数。分析两组肿瘤细胞的放射性计数及肿瘤组织的放射性摄取值(%ID/g)。结果成功制备^(18)F-NT,其理化性质及各项质控指标均达标。细胞结合实验显示,PC3细胞实验组计数值(5 825.00±1 074.52)/min,高于阻断组(1 941.66±173.58)/min,两者比较,差异有统计学意义(t=7.227,P=0.003),而对照组计数值仅为(170.33±56.59)/min;两组荷瘤裸鼠体内生物学分布实验表明,^(18)F-NT血液清除较快,主要经肾脏代谢。实验组肿瘤摄取值最高,为(1.02±0.49)%ID/g,阻断组肿瘤摄取值降低,为(0.21±0.03)%ID/g,两者比较,差异有统计学意义(t=2.815,P=0.049)。结论^(18)F-NT标记率和放化纯高,体外稳定性好,前列腺癌细胞系PC3结合^(18)F-NT高,PC3荷瘤裸鼠的肿瘤摄取^(18)F-NT高,细胞和荷瘤均能被NT有效阻断,为后续^(18)F-NT靶向前列腺癌NTR1在体显像打下良好的实验基础。 展开更多
关键词 ^18F-NT 前列腺癌 PC3 细胞结合实验 生物学分布实验
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Zircon U-Pb ages and provenance characteristics of the Zhiluo Formation sandstones and the formation background of the uranium deposit in Huangling area,Ordos Basin,China 被引量:1
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作者 Reng-an Yu Shan-bo Wang +5 位作者 Qiang Zhu Qing-hong Si Xue-ming Teng Xiao-xue Liu Hou-ning Liu yong-xiang tang 《China Geology》 2021年第4期600-615,共16页
The newly discovered medium-scale Huangling uranium deposit is located in the Shuanlong area of the southeast Ordos Basin.This paper presents the systematic geochemical and zircon U-Pb studies on the Zhiluo Formation ... The newly discovered medium-scale Huangling uranium deposit is located in the Shuanlong area of the southeast Ordos Basin.This paper presents the systematic geochemical and zircon U-Pb studies on the Zhiluo Formation sandstones in the Huanling area.The data obtained play an important role in deducing the provenance and tectonic setting of the source rocks.The results show that the lower part of the Zhiluo Formation is mainly composed of felsic sedimentary rocks.The source rocks originated from a continental island arc environment in terms of tectonic setting.U-Pb ages of detrital zircons obtained can be roughly divided into three groups:170‒500 Ma,1600‒2050 Ma,and 2100‒2650 Ma.Based on the characteristics of trace elements and rare earth elements(REE)and the zircon U-Pb dating results,it is considered that the Cryptozoic Edo provenance of the Zhiluo Formation mainly includes magmatic rocks(such as granodioritic intrusions)and metamorphic rocks(such as gneiss and granulite)in the orogenic belts on the northern margin of the North China Plate and in the Alxa Block.Based on sedimentological and petrological results,it can be concluded that the provenance of clastic sediments in the Zhiluo Formation was in north-south direction.The preconcentration of uranium is relatively low in the Lower Zhiluo Formation in the Huangling area.Meanwhile,the paleocurrent system in the sedimentary period is inconsistent with the ore-bearing flow field in the mineralization period,which restricts the formation of large-scale and super-large-scale uranium deposits and ore zones in the southeast Ordos Basin.The understanding of provenance directions will provide crucial references for the Jurassic prototype recovery and paleo-geomorphology of the Ordos Basin and the prediction of potential uranium reservoirs of the basin. 展开更多
关键词 Detrital zircon U-Pb geochronology PROVENANCE Uranium deposit Sandstone type Zhiluo Formation Huangling area Mineral exploration engineering Ordos Basin China
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