The variation of membrane surface and lateral diffusion of membrane protein was studied after the interaction of laminin with its membrane receptor in mouse macrophages. A pattern of membrane surface which showed smal...The variation of membrane surface and lateral diffusion of membrane protein was studied after the interaction of laminin with its membrane receptor in mouse macrophages. A pattern of membrane surface which showed smaller and bigger peaks was obtained by scanning tunneling microscope(STM), looking like the domains of lipid groups and proteins in the model of fluid mosaic biomembrane. Some even more higher and wider peaks projected out from the membrane surface in STM image after the interacting of laminin with membrane receptor were, probably, the complexes of laminin and membrane receptor. Furthermore, the decreased lateral diffusion coefficient value (D) was obtained by fluorescence recovery after photobleaching (FRAP) after the laminin was reacted with membrane receptor. This phenomenon provides an evidence that the complexes of laminin and its membrane receptor were located on the membrane of macrophages. So we could consider that the laminin is combined with membrane receptor leading to the variation in the properties of membrane surface.展开更多
Specific cellular immune tolerance may be essential for successful xenotransplantation in humans.Thymecto- mized (ATX),T and NK cell-depleted immunocompetent mice grafted with xenogeneic fetal pig thymic and liver tis...Specific cellular immune tolerance may be essential for successful xenotransplantation in humans.Thymecto- mized (ATX),T and NK cell-depleted immunocompetent mice grafted with xenogeneic fetal pig thymic and liver tissue (FP THY/LIV) result in efficient mouse thymopoiesis and peripheral repopulation of functional mouse CD4^+ T cell.Very importantly,the reconstituted mouse T cells are specifically tolerant to pig donor antigens.Studies demonstrated that porcine MHCs mediated positive and negative selection of mouse thymocytes in FP THY grafts,whereas mouse MHCs were involved in negative selection in grafts.Therefore,T cell tolerance to xenogeneic donor antigens could be induced by grafting donor thymus tissue.Xenogeneic thymic replacement might have a potential role in the reconstitution of cellular immunity in patients with AIDS or other immunodeficiencies caused by thymus dysfunction.Cellular & Molecular Immunology.2004;1(3): 173-179.展开更多
基金a great from the Nationa Natural Science Foundation,
文摘The variation of membrane surface and lateral diffusion of membrane protein was studied after the interaction of laminin with its membrane receptor in mouse macrophages. A pattern of membrane surface which showed smaller and bigger peaks was obtained by scanning tunneling microscope(STM), looking like the domains of lipid groups and proteins in the model of fluid mosaic biomembrane. Some even more higher and wider peaks projected out from the membrane surface in STM image after the interacting of laminin with membrane receptor were, probably, the complexes of laminin and membrane receptor. Furthermore, the decreased lateral diffusion coefficient value (D) was obtained by fluorescence recovery after photobleaching (FRAP) after the laminin was reacted with membrane receptor. This phenomenon provides an evidence that the complexes of laminin and its membrane receptor were located on the membrane of macrophages. So we could consider that the laminin is combined with membrane receptor leading to the variation in the properties of membrane surface.
文摘Specific cellular immune tolerance may be essential for successful xenotransplantation in humans.Thymecto- mized (ATX),T and NK cell-depleted immunocompetent mice grafted with xenogeneic fetal pig thymic and liver tissue (FP THY/LIV) result in efficient mouse thymopoiesis and peripheral repopulation of functional mouse CD4^+ T cell.Very importantly,the reconstituted mouse T cells are specifically tolerant to pig donor antigens.Studies demonstrated that porcine MHCs mediated positive and negative selection of mouse thymocytes in FP THY grafts,whereas mouse MHCs were involved in negative selection in grafts.Therefore,T cell tolerance to xenogeneic donor antigens could be induced by grafting donor thymus tissue.Xenogeneic thymic replacement might have a potential role in the reconstitution of cellular immunity in patients with AIDS or other immunodeficiencies caused by thymus dysfunction.Cellular & Molecular Immunology.2004;1(3): 173-179.
