In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson＇s Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesen...In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson＇s Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells against 6-hydroxydopamine-induced neurotoxicity and to verify whether the mechanism of action relates to abnormal a-synuclein accumulation in cells Results showed that co-culture with bone marrow-derived mesenchymal stem cells enhanced PC12 cell viability and dopamine secretion in a cell dose-dependent manner. MitoLight staining was used to confirm that PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells demonstrate reduced levels of cell apoptosis. Immunocytochemistry and western blot analysis found the quantity of α-synuclein accumulation was significantly reduced in PC12 cell and bone marrow-derived mesenchymal stem cell co-cultures. These results indicate that bone marrow-derived mesenchymal stem cells can attenuate 6-hydroxydopamine-induced cytotoxicity by reducing abnormal α-synuclein accumulation in PC12 cells.展开更多
The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits t...The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium aiginate microspheres （100-300 pm）, a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the dght hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models.展开更多
Objective To elucidate the transformation of energy metabolism patterns in the process of myocardial remodeling induced by volumeoverload and to explore a novel intervention target for the prevention,delay or even rev...Objective To elucidate the transformation of energy metabolism patterns in the process of myocardial remodeling induced by volumeoverload and to explore a novel intervention target for the prevention,delay or even reversal of structural heart dysfunction.Methods Thirty C57/BL6 mice,20-30 g,half male and half female,were randomly divided into model group(n=15)and sham operation group(n=15).Each group was divided into subacute phase(2 weeks after surgery,n=5),cardiac functional compensation phase(5 weeks after surgery,n=5)and decompensation phase(15 weeks after surgery,n=5).展开更多
Objective Mitochondria serve as energy generators, and its energy metabolism disorder is a key factor in myocardial remodeling. The purpose of this study was to investigate the role of PINK1-Mfn2-Parkinmediated mitoch...Objective Mitochondria serve as energy generators, and its energy metabolism disorder is a key factor in myocardial remodeling. The purpose of this study was to investigate the role of PINK1-Mfn2-Parkinmediated mitochondrial autophagy in the development and progression of myocardial remodeling, which is important for mitochondrial quality control and maintenance of cellular energy metabolism homeostasis.展开更多
Objective Our aim is to construct an indicator system for the graduate dissertation quality evaluation,based on Fuzzy Analytical Hierarchy Process(FAHP).Methods First,we reviewed the related literature to identify and...Objective Our aim is to construct an indicator system for the graduate dissertation quality evaluation,based on Fuzzy Analytical Hierarchy Process(FAHP).Methods First,we reviewed the related literature to identify and select possible factors.Second,we developed an analytic hierarchy process framework.展开更多
Traditionally, the materials which are regarded as antigens recognized by γδ T lymphocytes are protein and carbohydrate, not nucleic acid or lipid. Recently, it has been demonstrated that γδ T cells can recognize ...Traditionally, the materials which are regarded as antigens recognized by γδ T lymphocytes are protein and carbohydrate, not nucleic acid or lipid. Recently, it has been demonstrated that γδ T cells can recognize lipid A and directly induce immune responses that involve CD1 （cluster of differentiation type 1） family and Toll like receptors （TLRs）. This is a review about the interacting-mechanism, immunological effect and clinical application of them.展开更多
基金supported by the Science and Technology Development Foundation of Beijing Science and Technology Commission, No. Z101107052210004
文摘In the present study, PC12 cells induced by 6-hydroxydopamine as a model of Parkinson＇s Disease, were used to investigate the protective effects of bone marrow-derived mesenchymal stem cells bone marrow-derived mesenchymal stem cells against 6-hydroxydopamine-induced neurotoxicity and to verify whether the mechanism of action relates to abnormal a-synuclein accumulation in cells Results showed that co-culture with bone marrow-derived mesenchymal stem cells enhanced PC12 cell viability and dopamine secretion in a cell dose-dependent manner. MitoLight staining was used to confirm that PC12 cells co-cultured with bone marrow-derived mesenchymal stem cells demonstrate reduced levels of cell apoptosis. Immunocytochemistry and western blot analysis found the quantity of α-synuclein accumulation was significantly reduced in PC12 cell and bone marrow-derived mesenchymal stem cell co-cultures. These results indicate that bone marrow-derived mesenchymal stem cells can attenuate 6-hydroxydopamine-induced cytotoxicity by reducing abnormal α-synuclein accumulation in PC12 cells.
基金supported by the Science and Technology Support Program of Beijing Science and Technology Committee,No.Z101107052210004
文摘The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium aiginate microspheres （100-300 pm）, a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the dght hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models.
文摘Objective To elucidate the transformation of energy metabolism patterns in the process of myocardial remodeling induced by volumeoverload and to explore a novel intervention target for the prevention,delay or even reversal of structural heart dysfunction.Methods Thirty C57/BL6 mice,20-30 g,half male and half female,were randomly divided into model group(n=15)and sham operation group(n=15).Each group was divided into subacute phase(2 weeks after surgery,n=5),cardiac functional compensation phase(5 weeks after surgery,n=5)and decompensation phase(15 weeks after surgery,n=5).
文摘Objective Mitochondria serve as energy generators, and its energy metabolism disorder is a key factor in myocardial remodeling. The purpose of this study was to investigate the role of PINK1-Mfn2-Parkinmediated mitochondrial autophagy in the development and progression of myocardial remodeling, which is important for mitochondrial quality control and maintenance of cellular energy metabolism homeostasis.
文摘Objective Our aim is to construct an indicator system for the graduate dissertation quality evaluation,based on Fuzzy Analytical Hierarchy Process(FAHP).Methods First,we reviewed the related literature to identify and select possible factors.Second,we developed an analytic hierarchy process framework.
基金supported by grants(2001CB510009 and 2004CB518706)from The National Program for Key Basic Research Projecta grant(NO.30490244)from National Natural Science Foundation of China.
文摘Traditionally, the materials which are regarded as antigens recognized by γδ T lymphocytes are protein and carbohydrate, not nucleic acid or lipid. Recently, it has been demonstrated that γδ T cells can recognize lipid A and directly induce immune responses that involve CD1 （cluster of differentiation type 1） family and Toll like receptors （TLRs）. This is a review about the interacting-mechanism, immunological effect and clinical application of them.
