分别采用氮气吹干法和旋转蒸发法制备由磷脂和膜支架蛋白组成的电荷型纳米盘,用凝胶过滤色谱对其尺寸分级,分析了其性能,考察了其与肝微粒体细胞色素P450的结合能力。结果表明,纳米盘外观澄清透明,微观呈圆盘状,平均直径10nm,在p H 7.4...分别采用氮气吹干法和旋转蒸发法制备由磷脂和膜支架蛋白组成的电荷型纳米盘,用凝胶过滤色谱对其尺寸分级,分析了其性能,考察了其与肝微粒体细胞色素P450的结合能力。结果表明,纳米盘外观澄清透明,微观呈圆盘状,平均直径10nm,在p H 7.4下Zeta电位为-19.86 m V;肝微粒体破碎液与纳米盘能很好结合,CO差示光谱在450 nm出现明显吸收峰,细胞色素P450含量为0.10 nmol/mg,比活比未经纳米盘处理时提高13.0倍,较传统方法提升1.5倍,且操作时间由数日缩短至数小时。电荷型纳米盘在结合膜蛋白细胞色素P450的同时,活性保持良好,在膜蛋白研究领域极具应用潜力。展开更多
Globally,copper(Cu)accumulation in soils is a major environmental concern.Agricultural organic waste and some bacterial species can readily absorb metals in an eco-friendly manner,and thus are commonly used in metal-c...Globally,copper(Cu)accumulation in soils is a major environmental concern.Agricultural organic waste and some bacterial species can readily absorb metals in an eco-friendly manner,and thus are commonly used in metal-contaminated soil remediation.This study investigates the change in Cu fractions during the aging process and the time effects of rice straw(RS)and engineered bacteria(EB)(Pseudomonas putida X4/pIME)on reduction of Cu mobility.Three typical Chinese soils(red,cinnamon,and black soils)were incubated with RS or RS+EB in the presence of exogenous Cu for 24 months.The soil physicochemical properties,reactive soil components,Cu fractions,and Cu mobility were determined over time.The Cu mobility factor(MF)values were the lowest in the black soil(6.4-9.2)because of its high organic carbon and clay contents.The additions of both RS and RS+EB accelerated Cu stabilization during the aging process in all three soils.The Cu MF values decreased with time during the initial 20 months;however,the MF values increased thereafter in all soils,which might be due to the reduction of humic substances and amorphous iron oxides and the increase in iron oxides complexed on the organic matter.The reduction rates of Cu MF were similar after 16,24,and more than 24 months in the red,cinnamon,and black soils,respectively,indicating that RS and RS+EB could limit Cu mobility at different times in various soils.The RS treatment showed the greatest efficiency in reducing Cu mobility in the red,cinnamon,and black soils after 12,12,and 8 months of incubation,respectively.The RS+EB treatment was more efficient than the RS treatment in the red soil during the initial 8 months of the incubation period.Our study provides theoretical support for Cu risk assessments and RS supplementation for Cu remediation in different soils.展开更多
文摘分别采用氮气吹干法和旋转蒸发法制备由磷脂和膜支架蛋白组成的电荷型纳米盘,用凝胶过滤色谱对其尺寸分级,分析了其性能,考察了其与肝微粒体细胞色素P450的结合能力。结果表明,纳米盘外观澄清透明,微观呈圆盘状,平均直径10nm,在p H 7.4下Zeta电位为-19.86 m V;肝微粒体破碎液与纳米盘能很好结合,CO差示光谱在450 nm出现明显吸收峰,细胞色素P450含量为0.10 nmol/mg,比活比未经纳米盘处理时提高13.0倍,较传统方法提升1.5倍,且操作时间由数日缩短至数小时。电荷型纳米盘在结合膜蛋白细胞色素P450的同时,活性保持良好,在膜蛋白研究领域极具应用潜力。
基金supported by the National Key R&D Program of China(No.2019YFC1605600)the National Natural Science Foundation of China(No.32072662)+5 种基金the National Youth Natural Science Foundation(No.4180071811)the Natural Science Foundation of Guangdong Province,China(No.2020A1515010819)the Doctor-Initiated Project of the Public Monitoring Center for Agro-Product of Guangdong Academy of Agricultural Sciences,China(No.ZXRC201903)the President Foundation of Guangdong Academy of Agricultural Sciences,China(No.202017)the Special Found for Scientific Innovation Strategy-Construction of High Level Academy of Agriculture Science,China(No.R2021YJ-QG006)the Foundation Project of Director of Institute of Quality Standard and Monitoring Technology for Agro-Products of Guangdong Academy of Agricultural Sciences,China(No.DWJJ-202113)。
文摘Globally,copper(Cu)accumulation in soils is a major environmental concern.Agricultural organic waste and some bacterial species can readily absorb metals in an eco-friendly manner,and thus are commonly used in metal-contaminated soil remediation.This study investigates the change in Cu fractions during the aging process and the time effects of rice straw(RS)and engineered bacteria(EB)(Pseudomonas putida X4/pIME)on reduction of Cu mobility.Three typical Chinese soils(red,cinnamon,and black soils)were incubated with RS or RS+EB in the presence of exogenous Cu for 24 months.The soil physicochemical properties,reactive soil components,Cu fractions,and Cu mobility were determined over time.The Cu mobility factor(MF)values were the lowest in the black soil(6.4-9.2)because of its high organic carbon and clay contents.The additions of both RS and RS+EB accelerated Cu stabilization during the aging process in all three soils.The Cu MF values decreased with time during the initial 20 months;however,the MF values increased thereafter in all soils,which might be due to the reduction of humic substances and amorphous iron oxides and the increase in iron oxides complexed on the organic matter.The reduction rates of Cu MF were similar after 16,24,and more than 24 months in the red,cinnamon,and black soils,respectively,indicating that RS and RS+EB could limit Cu mobility at different times in various soils.The RS treatment showed the greatest efficiency in reducing Cu mobility in the red,cinnamon,and black soils after 12,12,and 8 months of incubation,respectively.The RS+EB treatment was more efficient than the RS treatment in the red soil during the initial 8 months of the incubation period.Our study provides theoretical support for Cu risk assessments and RS supplementation for Cu remediation in different soils.
