Protein-stabilized nanoemulsion was successfully prepared by ultrasound-assisted method under optimal ultrasound conditions,so as to improve the defects of sea buckthorn fruit oil(SBFO)and increase its bioavailability...Protein-stabilized nanoemulsion was successfully prepared by ultrasound-assisted method under optimal ultrasound conditions,so as to improve the defects of sea buckthorn fruit oil(SBFO)and increase its bioavailability.This paper investigated the performance of nanoemulsion obtained by sodium caseinate(SC)and whey glycoprotein(WGP)at different concentrations,including polymer dispersity index(PDI),particle size,zeta potential(ZP).The SBFO nanoemulsion prepared under the optimal concentration of protein has good microstructure and rheological properties.Under different temperature(20-80℃),pH(3-9),salinity(50-200 mM)and storage time(4 weeks),the performance of SBFO nanoemulsion has been measured to prove that SBFO nanoemulsion can maintain stable state in most cases.The safety of the SBFO nanoemulsion was evaluated by cytotoxicity(MTT)method and the results showed non-toxic to normal human embryonic kidney cells(HEK 293T).展开更多
基金This work is financially supported by Science and Technology Planning Project of Guangzhou Science and Technology Bureau(No.202102080365)Science and Technology Planning Project of Guangdong Science and Technology Department(No.KTP20200170)+4 种基金Yunfu City Science and Technology Plan Project(No.2020020101)Heyuan City Science and Technology Plan Project(No.2018014)Natural Science Foundation of Guangdong Province,China(No.2018A030310477)Zhongshan Social Welfare and basic research program(No.2020B2005)Guangdong MEPP Fund(No.GDOE[2019]A27).
文摘Protein-stabilized nanoemulsion was successfully prepared by ultrasound-assisted method under optimal ultrasound conditions,so as to improve the defects of sea buckthorn fruit oil(SBFO)and increase its bioavailability.This paper investigated the performance of nanoemulsion obtained by sodium caseinate(SC)and whey glycoprotein(WGP)at different concentrations,including polymer dispersity index(PDI),particle size,zeta potential(ZP).The SBFO nanoemulsion prepared under the optimal concentration of protein has good microstructure and rheological properties.Under different temperature(20-80℃),pH(3-9),salinity(50-200 mM)and storage time(4 weeks),the performance of SBFO nanoemulsion has been measured to prove that SBFO nanoemulsion can maintain stable state in most cases.The safety of the SBFO nanoemulsion was evaluated by cytotoxicity(MTT)method and the results showed non-toxic to normal human embryonic kidney cells(HEK 293T).
