Insect pest damage to crops is a threat to global food security(Tilman et al.,2011).Climate change,the evolution of insecticide resistance,and the phasing out of insecticides due to environmental and safety concerns e...Insect pest damage to crops is a threat to global food security(Tilman et al.,2011).Climate change,the evolution of insecticide resistance,and the phasing out of insecticides due to environmental and safety concerns exacerbate this problem.Farmers urgently need safe and effective crop protection tools to sustainably generate yields that meet ever-increasing global demand.展开更多
Polydnaviruses （PDVs） are a group of insect DNA viruses, which exhibit a mutual symbiotic relationship with their specific host wasps. Moreover, most encapsidated genes identified so far in PDVs share homologies wit...Polydnaviruses （PDVs） are a group of insect DNA viruses, which exhibit a mutual symbiotic relationship with their specific host wasps. Moreover, most encapsidated genes identified so far in PDVs share homologies with insect-originated genes, but not with virus-originated genes. In the meantime, PDVs associated with 2 wasp genera Cotesia and Glytapanteles encode some genes presumably originated from other viruses. Cotesia plutellae bracovirus （CpBV） encodes 4 genes homologous to baculoviral p94： CpBV- E94k1, CpB V-E94k2, CpB V-E94k3, and CpB V-E94k4. This study was conducted to predict the origin of CpB V-E94ks by comparing their sequences with those ofbaculoviral orthologs and to determine the physiological functions by their transient expressions in nonparasitized larvae and subsequent specific RNA interference. Our phylogenetic analysis indicated that CpBV-E94ks were clustered with other E94ks originated from different PDVs and shared high similarity with betabaculoviral p94s. These 4 CpBV genes were expressed during most developmental stages of the larvae of Plutella xylostella parasitized by C. plutellae. Expression of these 4 E94ks was mainly detected in hemocytes and fat body. Subsequent functional analysis by in vivo transient expression showed that all 4 viral genes significantly inhibited both host immune and developmental processes. These results suggest that CpB 11- E94ks share an origin with betabaculoviral p94s and play parasitic roles in suppressing host immune and developmental processes.展开更多
基金supported by the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences(CAAS-2060302).
文摘Insect pest damage to crops is a threat to global food security(Tilman et al.,2011).Climate change,the evolution of insecticide resistance,and the phasing out of insecticides due to environmental and safety concerns exacerbate this problem.Farmers urgently need safe and effective crop protection tools to sustainably generate yields that meet ever-increasing global demand.
文摘Polydnaviruses （PDVs） are a group of insect DNA viruses, which exhibit a mutual symbiotic relationship with their specific host wasps. Moreover, most encapsidated genes identified so far in PDVs share homologies with insect-originated genes, but not with virus-originated genes. In the meantime, PDVs associated with 2 wasp genera Cotesia and Glytapanteles encode some genes presumably originated from other viruses. Cotesia plutellae bracovirus （CpBV） encodes 4 genes homologous to baculoviral p94： CpBV- E94k1, CpB V-E94k2, CpB V-E94k3, and CpB V-E94k4. This study was conducted to predict the origin of CpB V-E94ks by comparing their sequences with those ofbaculoviral orthologs and to determine the physiological functions by their transient expressions in nonparasitized larvae and subsequent specific RNA interference. Our phylogenetic analysis indicated that CpBV-E94ks were clustered with other E94ks originated from different PDVs and shared high similarity with betabaculoviral p94s. These 4 CpBV genes were expressed during most developmental stages of the larvae of Plutella xylostella parasitized by C. plutellae. Expression of these 4 E94ks was mainly detected in hemocytes and fat body. Subsequent functional analysis by in vivo transient expression showed that all 4 viral genes significantly inhibited both host immune and developmental processes. These results suggest that CpB 11- E94ks share an origin with betabaculoviral p94s and play parasitic roles in suppressing host immune and developmental processes.
