Optimal electromagnetic hybrid negative current compensation method for high-speed railway power supply system

To achieve economical compensation for the huge-capacity negative sequence currents generated by high-speed railway load, an electromagnetic hybrid compensation system(EHCS) and control strategy is proposed.The EHCS is made up of a small-capacity railway static power conditioner(RPC) and a large-capacity magnetic static var compensator(MSVC). Compared with traditional compensation methods, the EHCS makes full use of the SVC’s advantages of economy and reliability and of RPC’s advantages of technical capability and flexibility. Based on the idea of injecting a negative sequence, the compensation principle of the EHCS is analyzed in detail. Then the minimum installation capacity of an EHCS is theoretically deduced. Furthermore, a constraint optimization compensation strategy that meets national standards, which reduces compensation capacity further, is proposed. An experimental platform based on a digital signal processor(DSP) and a programmable logic controller(PLC) is built to verify the analysis. Simulated and experimental results are given to demonstrate the effectiveness and feasibility of the proposed method.

Pareto optimal allocation of fault current limiter based on immune algorithm considering cost and mitigation effect

This paper presents a multi-objective Pareto optimal method for allocation of fault current limiters based on an immune algorithm, which takes into account two objectives of the cost and fault current mitigation effect. A sensitivity factor calculation method based on the rate of fault current mitigation is proposed to reduce the search space and improve the efficiency of the algorithm.In this approach, the objective functions related to the cost and fault current mitigation effect are established. A modified inversion operator based on equal cost is proposed to converge to global optimal solutions more effectively. The proposed algorithm is tested on the IEEE39-bus system, and obtains the Pareto optimal solutions,from which the user can select the most suitable solutions according to the preferences and relative importance of the objective functions. Simulation results are used to verify the proposed method.

Non-Structural Protein 5 of Zika Virus Interacts with p53 in Human Neural Progenitor Cells and Induces p53-Mediated Apoptosis

Zika virus(ZIKV) infection could disrupt neurogenesis and cause microcephaly in neonates by targeting neural progenitor cells(NPCs). The tumor suppressor p53-mediated cell cycle arrest and apoptotic cell death have been suggested to be activated upon ZIKV infection, yet the detailed mechanism is not well understood. In the present study, we investigated the effects of ZIKV-encoded proteins in the activation of p53 signaling pathway and found that, among the ten viral proteins,the nonstructural protein 5(NS5) of ZIKV most significantly activated the transcription of p53 target genes. Using the immunoprecipitation-coupled mass spectrometry approach, we identified that ZIKV-NS5 interacted with p53 protein. The NS5-p53 interaction was further confirmed by co-immunoprecipitation and GST pull-down assays. In addition, the MTase domain of NS5 and the C-terminal domain of p53 were mapped to be responsible for the interaction between these two proteins. We further showed that ZIKV-NS5 was colocalized with p53 and increased its protein level in the nuclei and able to prolong the half-life of p53. Furthermore, lentivirus-mediated expression of ZIKV-NS5 in hNPCs led to an apparent cell death phenotype. ZIKV-NS5 promoted the cleavage of PARP1 and significantly increased the cell apoptosis of h NPCs.Taken together, these findings revealed that ZIKV-NS5 is a previously undiscovered regulator of p53-mediated apoptosis in hNPCs, which may contribute to the ZIKV-caused abnormal neurodevelopment.

A novel phosphorylation site in SARS-CoV-2 nucleocapsid regulates its RNA-binding capacity and phase separation in host cells

Dear Editor,The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a novel emerging coronavirus that has spread worldwide since breaking out in late 2019 and has led to hundreds of millions of infections and millions of human deaths(Zhou et al.,2020).The genome of SARS-CoV-2 encodes 29 viral proteins.