Objectives The work intended to reveal the effect of cold shock(CS)treatment on chiling injury(CI),antioxidant capacity.and membrane fatty acid of peach fruit.Materials and methods Peaches were soaked in ice water(10...Objectives The work intended to reveal the effect of cold shock(CS)treatment on chiling injury(CI),antioxidant capacity.and membrane fatty acid of peach fruit.Materials and methods Peaches were soaked in ice water(10℃)for 10 min and stored at 5℃ for 28 d for determination,except CI,and then stored for 3 dat 20℃,only CI was measured.The electrolyte leakage(EL)was measured by conductivity meter.The activities of antioxidant enzymes(superoxide dismutase,ascorbate peroxidase,catalase,and peroxidase)and key enzymes of membrane lipid metabolism(phospho-lipase D,lipase,and lipoxygenase)as well as reactive oxygen species(ROS,O_(2)^(-)and H_(2)O_(2))were measured with a spectrophotometer.An ELISA kit and gas chromatography were used to determine membrane lipids and membrane fatty acids.The relative gene expression was measured by real-time polymerase chain reaction analysis.Results The results showed that CS treatment effectively delayed CI,suppressed the increase of EL and malondialdehyde content.Meanwhile,CS-treated fruit exhibited lower level of ROS and higher activities of antioxidant enzymes.Furthermore,CS treatment inhibited the activities as well as the relative gene expression of key enzymes in membrane lipid metabolism.CS-treated fruits maintained higher membrane fatty acid unsaturation and lower phosphatidic acid content.Conclusions These results indicated that CS treatment effectively lleviated CI and maintained the integrity of cell membranes by inducing antioxidant-related enzyme activity and maintaining a higher ratio of unsaturated fatty acids to saturated fatty acids.展开更多
Cold storage of fruits is the best strategy to extend shelf life.However,storage temperature below 8℃ mostly causes chilling injury(CI).Thus,exogenous melatonin treatment is an alternative safe preservative to allevi...Cold storage of fruits is the best strategy to extend shelf life.However,storage temperature below 8℃ mostly causes chilling injury(CI).Thus,exogenous melatonin treatment is an alternative safe preservative to alleviate CI and induce cold tolerance in postharvest of fruits and vegetables.This paper reviewed the recent progresses of melatonin-mediated physiological,biochemical and molecular mechanisms on chilling tolerance of fruits and vegetables.Among cold tolerance mechanisms,melatonin substantially enhances enzymatic and non-enzymatic antioxidants removing reactive oxygen species(ROS).Melatonin triggered phenol accumulation which associated with 2,2-diphenyl-1picryl hydrazyl(DPPH)scavenging capacity and reduction of oxidative damage.In addition,melatonin extensively maintained intracellular energy and enhanced energy regulation associated enzymes,thereby maintaining cellular stability.Melatonin induces unsaturated/saturated fatty acid(unSFA/SFA)ratio,improving cellular fl uidity.Besides,melatonin regulates arginine metabolisms via increased polyamines,proline andγ-aminobutyric acid(GABA)contents.Furthermore,melatonin possibly encodes calcium-dependent protein kinases(CDPK)and mitogen-activated protein kinases(MAPK)cascades.Moreover,it substantially induces Ca^(2+)signaling transduction pathways.As part of the signaling pathway,melatonin activates C-repeat binding factors(CBFs)as transcriptional factors for cold acclimation.Therefore,this review concluded that exogenous melatonin improves chilling stress tolerance by coordinating biochemical and molecular mechanisms at post-harvest life of fruits and vegetables and extending shelf life.展开更多
基金supported by the National Natural Science Foundation of China (No.31972125)the Fundamental Research Funds for the Central Universities (KYYJ201908),China.
文摘Objectives The work intended to reveal the effect of cold shock(CS)treatment on chiling injury(CI),antioxidant capacity.and membrane fatty acid of peach fruit.Materials and methods Peaches were soaked in ice water(10℃)for 10 min and stored at 5℃ for 28 d for determination,except CI,and then stored for 3 dat 20℃,only CI was measured.The electrolyte leakage(EL)was measured by conductivity meter.The activities of antioxidant enzymes(superoxide dismutase,ascorbate peroxidase,catalase,and peroxidase)and key enzymes of membrane lipid metabolism(phospho-lipase D,lipase,and lipoxygenase)as well as reactive oxygen species(ROS,O_(2)^(-)and H_(2)O_(2))were measured with a spectrophotometer.An ELISA kit and gas chromatography were used to determine membrane lipids and membrane fatty acids.The relative gene expression was measured by real-time polymerase chain reaction analysis.Results The results showed that CS treatment effectively delayed CI,suppressed the increase of EL and malondialdehyde content.Meanwhile,CS-treated fruit exhibited lower level of ROS and higher activities of antioxidant enzymes.Furthermore,CS treatment inhibited the activities as well as the relative gene expression of key enzymes in membrane lipid metabolism.CS-treated fruits maintained higher membrane fatty acid unsaturation and lower phosphatidic acid content.Conclusions These results indicated that CS treatment effectively lleviated CI and maintained the integrity of cell membranes by inducing antioxidant-related enzyme activity and maintaining a higher ratio of unsaturated fatty acids to saturated fatty acids.
基金supported by National Natural Science Foundation of China(31671901,31972125).
文摘Cold storage of fruits is the best strategy to extend shelf life.However,storage temperature below 8℃ mostly causes chilling injury(CI).Thus,exogenous melatonin treatment is an alternative safe preservative to alleviate CI and induce cold tolerance in postharvest of fruits and vegetables.This paper reviewed the recent progresses of melatonin-mediated physiological,biochemical and molecular mechanisms on chilling tolerance of fruits and vegetables.Among cold tolerance mechanisms,melatonin substantially enhances enzymatic and non-enzymatic antioxidants removing reactive oxygen species(ROS).Melatonin triggered phenol accumulation which associated with 2,2-diphenyl-1picryl hydrazyl(DPPH)scavenging capacity and reduction of oxidative damage.In addition,melatonin extensively maintained intracellular energy and enhanced energy regulation associated enzymes,thereby maintaining cellular stability.Melatonin induces unsaturated/saturated fatty acid(unSFA/SFA)ratio,improving cellular fl uidity.Besides,melatonin regulates arginine metabolisms via increased polyamines,proline andγ-aminobutyric acid(GABA)contents.Furthermore,melatonin possibly encodes calcium-dependent protein kinases(CDPK)and mitogen-activated protein kinases(MAPK)cascades.Moreover,it substantially induces Ca^(2+)signaling transduction pathways.As part of the signaling pathway,melatonin activates C-repeat binding factors(CBFs)as transcriptional factors for cold acclimation.Therefore,this review concluded that exogenous melatonin improves chilling stress tolerance by coordinating biochemical and molecular mechanisms at post-harvest life of fruits and vegetables and extending shelf life.
