The cotton mitochondrial chimeric gene orf610a causes male sterility by disturbing the dynamic balance of ATP synthesis and ROS burst

Plant cytoplasmic male sterility(CMS)is maternally inherited and often manifested as aborted pollen development,but the molecular basis of abortion remains to be identified.To facilitate an investigation of CMS in cotton,the complete sequence of cotton mitochondrial(mt)genome for CMS-D2 line ZBA was determined.The mt genome was assembled as a single circular molecule with 634,036 bp in length.A total of 194 ORFs,36 protein-coding genes,six r RNAs,and 24 t RNAs were identified.Several chimeric genes encoding hypothetical proteins with transmembrane domains were identified.Among them,a previously unknown chimeric gene,orf610a,which is composed of atp1 and a 485-bp downstream sequence of unknown nature,was identified.RT-PCR and q RT-PCR validation indicated that orf610a was expressed specifically in a sterile line.Ectopic expression of orf610a in yeast resulted in excessive accumulation of reactive oxygen species and reduction in ATP content,in addition to inhibition of cellular growth.Transgenic A.thaliana overexpressing orf610a fused with a mitochondrial targeting peptide displayed partial male sterility.Interaction between ORF610a and the nuclear-encoded protein RD22 indicated an association between ORF610a and pollen abortion.Positive feedback during transcriptional regulation between nuclear regulatory factors and the mt CMS gene may account for the male sterility of ZBA.

Mitochondrial genome of Chthamalus challengeri(Crustacea:Sessilia): gene order comparison within Chthamalidae and phylogenetic consideration within Balanomorpha

Acorn barnacles are important model species in researches on intertidal ecology, larval development and biofouling. At present, with the development of mitochondrial genomics, it is helpful to understand the phylogenetic relationship from the mitogenomic level. The complete mitochondrial genome of Chthamalus challengeri was presented. The genome is a circular molecule of 15 358 bp. Compared with other species in Balanomorpha, the non-coding region is longer, while the length of the genes is similar to the other species. The overall A+T content of the mitochondrial genome of C. challengeri is 70.5%. There are variations of initiation and stop codons in the known Balanomorpha mitochondrial genomes. The C. challengeri and C. antennatus within the same genus share the identical gene arrangement. However, the gene arrangement of different genera in Chthamalidae is different, as there is a translocation between two tRNA genes and an inversion involving a large gene block. In particular, both srRNA and lrRNA of the two species in Chthamalus are encoded in the heavy strand, differing from the former Balanomorpha species. The topology and gene arrangement in Chthamalidae support each other. Phylogenetic analysis indicates that the Chthamalidae is monophyletic, while the Balanidae and Archaeobalanidae are polyphyletic.

Application Effect of Detail Nursing in Neurosurgery Patients

Objective:To explore the effect of detail nursing in neurosurgery.Methods:72 cases of neurosurgery patients in our hospital from July 2018 to June 2020 were selected as the research sample,and they were divided into detail nursing group and routine nursing group,with 36 cases in each group.The retrospective analysis method was used to compare the effect of nursing intervention between the two groups.Results:The scores of anxiety(23.25±1.97*),depression(25.75±2.53*)and social activity ability(23.13±2.36*)in detail nursing group were higher than those in routine nursing group;The nursing satisfaction of detail nursing group was 35 cases(97.22%),significantly higher than that of routine nursing group 26 cases(72.22%),P<0.05,with statistical significance.Conclusion:Through the details of neurosurgery patients nursing means,to improve the prognosis of patients with recovery and improve the quality of life have significant effect,can reduce the complications of patients with prognosis,improve the patient's cognition of details of nursing,reduce the contradiction between doctors and patients,improve nursing satisfaction,has high clinical application and promotion value.

Ototoxic effects and mechanisms of loop diuretics

Over the past two decades considerable progress has been made in understanding the ototoxic effects and mechanisms underlying loop diuretics. As typical representative of loop diuretics ethacrynic acid or furosemide only induces temporary hearing loss, but rarely permanent deafness unless applied in severe acute or chronic renal failure or with other ototoxic drugs. Loop diuretic induce unique pathological changes in the cochlea such as formation of edematous spaces in the epithelium of the stria vascularis, which leads to rapid decrease of the endolymphatic potential and eventual loss of the cochlear microphonic potential, summating potential, and compound action potential. Loop diuretics interfere with strial adenylate cyclase and Nat/Kt-ATPase and inhibit the Na-K-2Cl cotransporter in the stria vascularis, however recent reports indicate that one of the earliest effects in vivo is to abolish blood flow in the vessels supplying the lateral wall. Since ethacrynic acid does not damage the stria vascularis in vitro, the changes in Nat/Kt-ATPase and Na-K-2Cl seen in vivo may be secondary effects results from strial ischemia and anoxia. Recent observations showing that renin is present in pericytes surrounding stria arterioles suggest that diuretics may induce local vasoconstriction by renin secretion and angiotensin formation. The tight junctions in the blood-cochlea barrier prevent toxic molecules and pathogens from entering cochlea, but when diuretics induce a transient ischemia, the barrier is temporarily disrupted allowing the entry of toxic chemicals or pathogens.

深部锚固节理岩体蠕变-疲劳破坏能量演化规律分析

为探究深部岩体在爆破、开挖等扰动荷载下的力学特性,开展锚固岩体在蠕变-疲劳荷载下的室内试验,并进一步探究其能量演化规律。结果表明:(1)在疲劳荷载作用下,加锚节理岩体比无锚条件下的滞回环面积小,在施加预应力时,其滞回环面积又大幅度减少,说明加锚预应力能有效降低其能量损耗;(2)岩石强度越高,其滞回环面积越小,对应的能量耗散越小,反之能量耗散大;(3)对比不同加卸载速率可知:在每一级循环荷载过程中,加卸载速率越大其对应的应变值、切线斜率就越大。考虑加卸载速率对节理岩体劣化作用,依据应变等价原理,得出节理岩体峰值损伤本构方程,并通过试验验证模型的准确性,为深部岩体结构支护提供思路。

A Simple SSR-based Method for Purity Identification of Cucurbita moschata Hybrids

Aiming at solving the existing issues in purity identification of Cucurbita moschata hybrids by SSR, such as complex operation and difficult application in production practice, in this study, a simple SSR-based method was established for purity identification of Cucurbita moschata hybrids. By using the established simple method, without grinding, freezing, centrifugation and drying, the genomic DNA extraction process is shorter than 3 min. Compared with the conventional method, PCR detection system and silver staining in polyacrylamide gel electrophoresis of the established method are more time-saving and cost-saving. The whole detection process is shorter than 4 h, and 480 samples can be detected with this method by one person in one day. In addition, the detection result exhibits a coincidence rate of 99% with field identification. The simple SSR-based method established in this study can provide basis for large-scale rapid purity identification of Cucurbita moschata hybrids.

Spirohypertones A and B as potent antipsoriatics:Tumor necrosis factor-αinhibitors with unprecedented chemical architectures

Tumor necrosis factor-α(TNF-α)is a promising target for inflammatory and autoimmune diseases.Spirohypertones A(1)and B(2),two unprecedented polycyclic polyprenylated acylphloroglucinols with highly rearranged skeletons,were isolated from Hypericum patulum.The structures of 1 and 2 were confirmed through comprehensive spectroscopic analysis,single-crystal X-ray diffraction and electronic circular dichroism calculations.Importantly,2 showed remarkable TNF-αinhibitory activity,which could protect L929 cells from death induced by co-incubation with TNF-αand actinomycin D.It also demonstrated the ability to suppress the inflammatory response in HaCaT cells stimulated with TNF-α.Notably,in an imiquimod-induced psoriasis murine model,2 restrained symptoms of epidermal hyperplasia associated with psoriasis,presenting anti-inflammatory and antiproliferative effects.This discovery positions 2 as a potent TNF-αinhibitor,providing a promising lead compound for developing an antipsoriatic agent.

Multi-Time-Scale Resource Allocation Based on Long-Term Contracts and Real-Time Rental Business Models for Shared Energy Storage Systems

The push for renewable energy emphasizes the need for energy storage systems(ESSs)to mitigate the unpre-dictability and variability of these sources,yet challenges such as high investment costs,sporadic utilization,and demand mismatch hinder their broader adoption.In response,shared energy storage systems(SESSs)offer a more cohesive and efficient use of ESS,providing more accessible and cost-effective energy storage solutions to overcome these obstacles.To enhance the profitability of SESSs,this paper designs a multi-time-scale resource allocation strategy based on long-term contracts and real-time rental business models.We initially construct a life cycle cost model for SESS and introduce a method to estimate the degradation costs of multiple battery groups by cycling numbers and depth of discharge within the SESS.Subsequently,we design various long-term contracts from both capacity and energy perspectives,establishing associated models and real-time rental models.Lastly,multi-time-scale resource allocation based on the decomposition of user demand is proposed.Numerical analysis validates that the business model based on long-term contracts excels over models operating solely in the real-time market in economic viability and user satisfaction,effectively reducing battery degradation,and leveraging the aggregation effect for SESS can generate an additional increase of 10.7%in net revenue.

空心环钻联合手术导板用于自体牙移植牙槽窝备洞

目的探讨空心环钻与数字化手术导板联合应用于制备自体牙移植受植窝洞的有效性及准确性。方法从2022年5—8月就诊于广州医科大学附属口腔医院颌面外科的患者中招募了16例磨牙缺失并接受自体牙移植修复的患者,实验对象采用随机数字表方法分为自由手组和导板组,每组8例。导板组用锥形束CT(CBCT)和口内扫描系统进行扫描,将结果导入Mimics 21.0,分析受体部位区域选择供体牙,模拟供体牙的植入以确定植入位置。设计基于空心环钻的数字化导板导向套,并生成数字化导板。自由手组按照常规自体牙移植手术流程,术中采用3D打印供牙模型试植,自由手预备窝洞。术后对患者重新进行CBCT扫描,使用Geomagic Control X匹配并对术前设计牙齿位置与术后实际牙齿位置进行比较。结果自体牙移植术后1年的随访中,两组的移植牙均成功愈合。导板组备洞时间为(323±50)s,自由手组为(522±91)s,两者相比差异有统计学意义(t=5.394,P<0.001)。导板组3D供牙试植次数(2.9±0.8)次,自由手组3D供牙试植次数(4.1±1.2)次,两者相比差异有统计学意义(t=2.357,P=0.034)。导板组术前术后牙齿的三维位置对比分析,显示牙体长轴的角度偏差为(9.0±5.5)°,3D偏差显示牙齿中心点的偏差为(1.0±0.6)mm,根尖偏差为(2.1±1.1)mm,移植牙相对于术前设计牙齿位置偏差较小。结论通过使用新方法,自体牙移植备洞时间缩短,过程可靠精确,深度可控,备洞流程简化,备洞过程中空心环钻取出骨块可用于自体牙移植牙槽骨缺损的骨移植,自体骨愈合效果好,节约成本。

颗粒床-旋流耦合分离器不同操作模式下的性能分析

通过实验分别考察了满床/空床操作模式对内置颗粒床-旋流耦合分离器分离性能的影响,获得了两种操作模式下的设备压降和捕集效率。通过改变入口粉尘浓度、入口气速和粉尘颗粒种类,发现满床操作条件下的分离效率比空床操作条件下的分离效率高,且前者压降较低。通过对出口粉尘粒径的分析,含有捕集颗粒的内置颗粒床可有效提高5μm以下的粉尘颗粒的捕集效率,弥补了离心分离的短板。引入性能指数对不同操作模式进行定量分析,验证了满床操作条件下的耦合分离设备具有更好的综合分离性能。

Prediction of Postbuckling Characteristics of Perforated Metamaterial Cylindrical Shells Under Axial Compression

Large deflection postbuckling responses of metamaterial cylindrical shells perforated by arrayed circular holes are investigated through a newly proposed theoretical model incorporating with finite element method and experiment.The triggering of an unusual pattern transformation under compressive load(that shows special hyperelastic metamaterial characteristics)contributes to the particularity of the postbucklingmodes,in which the axisymmetric waisted and non-axisymmetric postbuckling configurations of perforated cylindrical shells are identified.The transformations of postbucking modes are mainly affected by global outline sizes of the shell and local geometrical parameters of holes.The structural load-carrying capacity for the waisted postbuckling response suffers a sudden drop and recovers when the holes collapse.Comparatively,the shell would undergo a sustained fall off under non-axisymmetric postbuckling states.The negative Poisson’s ratio induced by pattern transformation plays a key role and a critical effective threshold value exists,inducing the waisted postbuckling mode.The revealed principles would be of benefit for achieving a controllable structural stability that is usually difficult to implement on those conventional structures.