Biomineralization of Se Nanoshpere by Bacillus Licheniformis

Biological dissimilatory reduction of selenite （SeO3^2-） to elemental selenium （Se^0） is common, but the mineral formation and the biogenic process remain uncertain. In this study, we examined the Se^0 formation during the selenite bioreduction by Bacillus licheniformis SeRB-1 through transmission electron microscope （TEM）, energy-dispersive spectrometry （EDS） and X-ray absorption fine structure （XAFS） techniques. Results showed that the reduction process occurred mostly during the exponential phase and early stationary phase, whilst the elemental selenium was produced in these periods. From the TEM images and polyaerylamide gel eleetropheresis, it is known that the Se^0 granule formation is a biologically-induced type, and the cell envelopes are the main biomineralization positions, and particles may go through a process from nucleation to crystallization, under the control of microbes. In fact, the minerals are spherical nanoparticles, occurring as a microcrystal or amorphous form. It is vital to recognize which kinds of proteins and/or polysaccharides act as a template to direct nanoparticle nucleation and growth？ This should focus for further studies. This study may shed light on the process of formation of Se（0） nanosphere.

Multi-GNSS products and services at iGMAS Wuhan Innovation Application Center:strategy and evaluation

Over the past years the International Global Navigation Satellite System(GNSS)Monitoring and Assessment System(iGMAS)Wuhan Innovation Application Center(IAC)dedicated to exploring the potential of multi-GNSS signals and providing a set of products and services.This contribution summarizes the strategies,achievements,and innovations of multi-GNSS orbit/clock/bias determination in iGMAS Wuhan IAC.Both the precise products and Real-Time Services(RTS)are evaluated and discussed.The precise orbit and clock products have comparable accuracy with the precise products of the International GNSS Service(IGS)and iGMAS.The multi-frequency code and phase bias products for Global Positioning System(GPS),BeiDou Navigation Satellite System(BDS),Galileo navigation satellite system(Galileo),and GLObal NAvigation Satellite System(GLONASS)are provided to support multi-GNSS and multi-frequency Precise Point Positioning(PPP)Ambiguity Resolution(AR).Compared with dual-frequency PPP AR,the time to first fix of triple-frequency solution is improved by 30%.For RTS,the proposed orbit prediction strategy improves the three dimensional accuracy of predicted orbit by 1 cm.The multi-thread strategy and high-performance matrix library are employed to accelerate the real-time orbit and clock determination.The results with respect to the IGS precise products show the high accuracy of RTS orbits and clocks,4–9 cm and 0.1–0.2 ns,respectively.Using real-time satellite corrections,real-time PPP solutions achieve satisfactory performance with horizontal and vertical positioning errors within 2 and 4 cm,respectively,and convergence time of 16.97 min.

Determination of global geodetic parameters using satellite laser ranging to Galileo,GLONASS,and BeiDou satellites

The new Global Navigation Satellite System(GNSS)satellites,including GLONASS,Galileo,and BeiDou system,are equipped with Laser Retroreflector Arrays(LRA)to support Satellite Laser Ranging(SLR)tracking,which contributes to the estimation of global geodetic parameters.In this study,we estimate the global geodetic parameters using the SLR observations to GNSS satellites and also investigate the effects of different data processing strategies on the estimated Earth Rotation Parameters(ERP),geocenter motion,and terrestrial scale.The results indicate that setting range bias parameters for each satellite-station pair can effectively account for the satellite-specific biases induced by LRAs,leading to smaller Root Mean Square Errors(RMSE)of the post-fit SLR residuals.Furthermore,estimating the range biases for each satellite-station pair improves the accuracy of the estimated station coordinates and ERP.We also examine the impact of different arc lengths on the estimates of ERP,geocenter motion,and terrestrial scale.The results show that extending arc length can significantly reduce the formal error of ERP.The 7-day strategy produces the smallest RMSEs of 473 microarcseconds and 495 microarcseconds for the estimated X-and Y-component of pole coordinates,and 52 microseconds for length-of-day,respectively.However,the estimated geocenter motion is less affected by the arc length,even the shortest 1-day arc strategy can capture the seasonal variations of geocenter motion in Z component.For scale estimation,extending the arc length notably improves the accuracy of the estimated station coordinates and scale,but this advantage becomes less noticeable in longer arcs.The 7-day solution also obtains the closet scale results compared to ITRF2014,with the RMSE of 2.10×10^(–9).

Orbit and clock products for quad-system satellites with undifferenced ambiguity fixing approach

Integer Ambiguity Resolution(IAR)can significantly improve the accuracy of GNSS Precise Orbit Determination(POD).Traditionally,the IAR in POD is achieved at the Double Differenced(DD)level.In this contribution,we develop an Un-Differenced(UD)IAR method for Global Positioning System(GPS)+BeiDou Navigation Satellite System(BDS)+Galileo navigation satellite system(Galileo)+Global'naya Navigatsionnaya Sputnikovaya Sistema(GLONASS)quad-system POD by calibrating UD ambiguities in the raw carrier phase and generating the so-called carrier range.Based on this method,we generate the UD ambiguity-fixed orbit and clock products for the Wuhan Innovation Application Center(IAC)of the International GNSS Monitoring and Assessment System(iGMAS).One-year observations in 2020 from 150 stations are employed to investigate performance of orbit and clock products.Notably,the UD Ambiguity Resolution(AR)yields more resolved integer ambiguities than the traditional DD AR,scaling up to 9%,attributable to its avoidance of station baseline formation.Benefiting from the removal of ambiguity parameters,the computational efficiency of parameter estimation undergoes a substantial 70%improvement.Compared with the float solution,the orbit consistencies of UD AR solution achieve the accuracy of 1.9,5.2,2.8,2.1,and 2.7 cm for GPS,BeiDou-2 Navigation Satellite System(BDS-2),BeiDou-3 Navigation Satellite System(BDS-3),Galileo,and GLONASS satellites respectively,reflecting enhancements of 40%,24%,54%,34%,and 42%.Moreover,the standard deviations of Satellite Laser Ranging(SLR)residuals are spanning 2.5–3.5 cm,underscoring a comparable accuracy to the DD AR solution,with discrepancies below 5%.A notable advantage of UD AR lies in its capability to produce the Integer Recovered Clock(IRC),facilitating Precise Point Positioning(PPP)AR without requiring additional Uncalibrated Phase Delay(UPD)products.To assess the performance of quad-system kinematic PPP based on IRC,a network comprising 120 stations is utilized.In comparison to the float solution,the IRC-based PPP AR accelerates convergence time by 31%and enhance positioning accuracy in the east component by 54%.