Complete genome sequence of the rifamycin SV-producing Amycolatopsis mediterranei U32 revealed its genetic characteristics in phylogeny and metabolism

Amycolatopsis mediterranei 被用于 rifamycin 的工业规模生产，它在 antimycobacterial 治疗起一个重要作用。作为类 Amycolatopsis 的首先定序的染色体， 236 715 底配对的包括 10 的紧张 U32 的染色体，最大的原核生物的染色体之一到目前为止曾经被定序。不同于在 streptomycetes 发现的线性拓扑学，这个染色体是圆形的，特别地类似于 Saccharopolyspora erythraea 和奴卡氏菌属 farcinica，在发展史和分类代表他们的靠近的关系。尽管在 A 预言了 9 228 编码蛋白质的基因。mediterranei 染色体 S 与那些分享了 orthologs 的最大的数字。erythraea，它被 Streptomyces coelicolor 而非 N 出人意料地跟随。farcinica，显示不同新陈代谢的特征经由改编演变到多样的生态的壁龛。除类似于那的一个核心区域以外在 streptomycetes 普通，有典型核心特征的一个新奇“伪核心”在非核心区域以内被定义，在 21 从 26 基因为第二等的代谢物生产聚类的总数被定位的地方。位于核心的 rifamycin 生合成基因簇为 rifamycin SV 的变换编码细胞色素 P450 酶必需品到 B，由比作 rifamycin 生产 B 紧张 S699 的高度相应的簇揭示了并且进一步由基因互补证实了。A 的 genomic 信息。mediterranei 表明在看起来复杂的规章的机制的控制下面不仅为各种各样的碳来源和无机的氮混合物的广泛的利用而且为新陈代谢的中介的有效 funneling 安排进第二等的抗菌素合成进程的一个新陈代谢的网络。

Analysis of the Transition Between Multiple Operational Modes for Hybrid AC/DC Microgrids

There are four basic operational modes for the hybrid AC/DC microgrid,including AC grid-connected while interconnecting,both off-grid while interconnecting,AC gridconnected without connection,and both off-grid without connection.How to achieve a seamless operational mode transition is an urgent technical need to overcome.First,this paper describes the typical structure of the hybrid microgrid,and places a detailed focus on the power balance and transition strategy.Secondly,it takes the master-slave control structure as an example,and designs the transition logic for different operational modes,and then a method for selecting the slack bus and transition time-sequence is proposed.Based on the different roles that the interlinking converter(IC)plays in the process of modes transition,a voltage-power(U-P)control method for a hybrid AC/DC microgrid is proposed,and the exchanged power is calculated based on the voltage deviation between the rating value and measured value.Finally,a control flowchart for the transition between the four operational modes in transition is designed.Using the PSCAD/EMTDC platform,this paper takes a typical seven-point microgrid structure as an example,the proposed transition strategy is carried out,and the results show that the transition method and time sequence can achieve smooth transition between different operational modes.

Status of testing field for ocean energy generation

Ocean energy, considered as clean energy, is one kind of marine resources of great importance. Its development and utilization have become an indispensable part of national development strategy in China. Testingfields are required when conducting tests of ocean energy generation devices in real sea, which is the key step conducted before engineering prototypes transformed into scale industrialization applications. This paper introduces the construction and operation conditions of large-scale testing fields for wave energy and tidal current energy generation devices and presents a brief comparative analysis. Developing status and related technologies on testingfields for ocean energy power generation in China are also discussed. Furthermore, this paper investigates the necessity of grid-connected test for ocean energy generation and points out that the construction of ocean energy testingfields is helpful to ensure the efficient utilization of ocean energy resources.

Significant sensing performance of an all-silicon terahertz metasurface chip for Bacillus thuringiensis Cry1Ac protein

The promising prospect of a terahertz metasurface in sensing and detection applications has attracted increasing attention because of its ability to overcome the classical diffraction limit and the enhancement of field intensity.In this work,a novel scheme based on an all-silicon terahertz plasmon metasurface is proposed and experimentally demonstrated to be a highly sensitive biosensor for the Bacillus thuringiensis Cry1Ac toxin.The regression coefficients between Bacillus thuringiensis protein concentrations and the spectral resonance intensity and frequency were 0.8988 and 0.9238,respectively.The resonance amplitude variation and frequency shift of the metasurface were investigated in terms of both thickness and permittivity change of the analyte,which reflected the protein residue in the actual process.Moreover,the reliability and stability of the metasurface chip were verified by time period,temperature,and humidity control.These results promise the ability of the proposed metasurface chip as a Bacillus thuringiensis protein sensor with high sensitivity and stability.In addition,this novel device strategy provides opportunities for the advancement of terahertz functional applications in the fields of biochemical sensing and detection.

Comprehensive evaluation method for tidal current power generation device

There are many types of tidal current power generation devices,and it is necessary to make comprehensive evaluation of tidal current power generation devices in order to provide valuable reference for the improvement of their performance indexes.On the basis of the analysis of the tidal current power generation device performance indexes,the hierarchical model for comprehensive evaluation of device performance is given in this paper.By normalizing the membership matrix elements based on fuzzy comprehensive evaluation model,all the values of the matrix elements are restrained in the range of0.0 to 1.0,hence the complexity of the calculations is reduced.Vector similarity is used to determine the expert weights which reflect the knowledge and experience of the experts.This paper presents an improved method for rank correlation analysis,and calculates the comprehensive weight value and the final evaluation results of tidal current power generation devices.The presented method improves the credibility of the evaluation.In the end,measured data of two units of tidal current power generation devices are evaluated in the paper,and the effectiveness of the presented method is verified.

Assessment of potential soybean cadmium excluder cultivars at different concentrations of Cd in soils

The selection of cadmium-excluding cultivars has been used to minimize the transfer of cadmium into the human food chain. In this experiment, five Chinese soybean plants were grown in three soils with different concentrations of Cd（0.15, 0.75 and 1.12 mg/kg）.Variations in uptake, enrichment, and translocation of Cd among these soybean cultivars were studied. The results indicated that the concentration of Cd in seeds that grew at1.12 mg/kg Cd in soils exceeded the permitted maximum levels in soybeans. Therefore, our results indicated that even some soybean cultivars grown on soils with permitted levels of Cd might accumulate higher concentrations of Cd in seeds that are hazardous to human health. The seeds of these five cultivars were further assessed for interactions between Cd and other mineral nutrient elements such as Ca, Cu, Fe, Mg, Mn and Zn. High Cd concentration in soil was found to inhibit the uptake of Mn. Furthermore, Fe and Zn accumulations were found to be enhanced in the seeds of all of the five soybean cultivars in response to high Cd concentration. Cultivar Tiefeng 31 was found to fit the criteria for a Cd-excluding cultivar under different concentrations of Cd in soils.