This paper describes a novel approach to the quality control of a Chinese herbal injection based on microca lorimetric determination of its effect on the biothermal active fingerprint(BTAF)of Escherichia coli(E.coli)g...This paper describes a novel approach to the quality control of a Chinese herbal injection based on microca lorimetric determination of its effect on the biothermal active fingerprint(BTAF)of Escherichia coli(E.coli)growth.Yinzhihuang Injection(YZHI)was selected for this proof of concept study.Reference samples of Y ZHI were collected and compared with test(stressed)samples prepared under different stress conditions.The BTAF of E.coli growth was found to be affected by YZHI and the changes were analyzed on the basis of eleven biothermokinetic parameters.Similarity and multivariate statistical analysis were used to investi-gate the differences between reference and test samples and discriminant analysis was used to delineate the altered samples.Reference samples were found to have coincident BTAFs with similarity index>0.99.Stressed samples showed differences in the BTAF which increased in line with decreased quality.Discriminant formulae were developed based on a sensitivity parameter which could identify all altered samples.In conclusion,BTAF can be used to assess the quality of YZHI both qualitatively and quantitatively and has the potential to provide a sensitive method for quality control of Chinese herbal injections.展开更多
基金supported by National Natural Science Foundation of China grants(No.81773893)National Major Scientific and Technological Special Project for“Significant New Drugs Development”(No.2017ZX09301060001)+2 种基金Hubei Province Key R&D Programme(No.2020BED017 and 2020BGB004)Jiangxi Province“Thousand Talents Plan”of Scientific and Technological Innovation(No.JXSQ2019201105)Fundamental Research Funds for the Central Universities“South-Central University for Nationalities”(No.CZP20025 and No.CZP20047)
基金The project was supported by the Natural Science Foundation of China(No.81173542)the China Military Logistics Scientific Research project(No.2010-89)the Special Foundation for Basic Scientific Research of Central Colleges(South-Central University for Nationalities,No.CZQ11038).
文摘This paper describes a novel approach to the quality control of a Chinese herbal injection based on microca lorimetric determination of its effect on the biothermal active fingerprint(BTAF)of Escherichia coli(E.coli)growth.Yinzhihuang Injection(YZHI)was selected for this proof of concept study.Reference samples of Y ZHI were collected and compared with test(stressed)samples prepared under different stress conditions.The BTAF of E.coli growth was found to be affected by YZHI and the changes were analyzed on the basis of eleven biothermokinetic parameters.Similarity and multivariate statistical analysis were used to investi-gate the differences between reference and test samples and discriminant analysis was used to delineate the altered samples.Reference samples were found to have coincident BTAFs with similarity index>0.99.Stressed samples showed differences in the BTAF which increased in line with decreased quality.Discriminant formulae were developed based on a sensitivity parameter which could identify all altered samples.In conclusion,BTAF can be used to assess the quality of YZHI both qualitatively and quantitatively and has the potential to provide a sensitive method for quality control of Chinese herbal injections.
