hoxY基因的克隆及其在节旋藻和螺旋藻系统学研究中的应用

从节旋藻属5个品系和螺旋藻属1个品系中克隆了hoxY基因的部分序列.序列长度都是479bp.在节旋藻中该基因GC含量为46.0%～46.6%,螺旋藻中为43.5%.节旋藻各品系间序列的相似性介于93.7%～100%,明显高于节旋藻属和螺旋藻属间的序列相似性(69.5%～72.2%).2个属中镍铁氢化酶小亚基HoxY氨基酸序列的比较也表明螺旋藻和节旋藻之间存在较大差异.利用MEGA2通过比较核酸序列构建了系统树,表明螺旋藻和节旋藻属处于不同的分枝.

Cloning and analysis of [NiFe]-hydrogenase genes from Arthrospira platensis FACHB341

The complete [ NiFe]-hydrogenase genes were cloned from cyanobacterium, Arthrospira platensis FACHB341, using PCR and in-vitro cloning. The total length is 2078bp, including hoxY 549bp, hoxH 1431bp and the spacer in between 98bp. There is a stem-loop structure, downstream of the hoxY gene, serving as a transcription terminator. The deduced amino acid sequences of HoxY aud HoxH consist of acidic amino acid of 15.8% and 12.0%; alkalitropic amino acid of 11.9% and 15.3%; hydrophobic amino acid of 46.3% and 41.0%, respectively. The similarities of hoxY and hoxH genes in Arthrospira platensis FACHB341 to their homologues in other cyanobacteria were compared respectively. The secondary structures and 3D models of small and large subunits of [ NiFe]-hydrogenase were predicted by using 3D-PSSM.

Hydrocarbons pyrolysed from nannoplanktonic algae:An experimental organism system for study on the origin of petroleum and natural gas

Enukuania huxleyi is marine nanoplanktonic alga with calcify scale called coccolith om its cell surface.This ubiquitous species with the Lafgest biomass in marice forms gigantic blooms covering thousands