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咪唑类离子液体长期暴露对鲤肠道形态、氧化应激、免疫和肠道菌群的影响
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作者 张艳敏 马丽美 +8 位作者 蔡慧敏 梁晓敏 游富 江爱霞 杨国坤 张新党 申亚伟 常绪路 孟晓林 《水生生物学报》 CAS CSCD 北大核心 2024年第2期193-203,共11页
以鲤(Cyprinus carpio L.)为受试对象,探究3种不同烷基链咪唑类离子液体(Alkyl ionic liquids,AILs)([C6mim]Cl、[C8mim]Cl和[C_(12)mim]Cl,暴露浓度分别为4.97、2.15和0.09 mg/L)对鲤肠道的毒性作用。结果表明随烷基链长度增加,与对照... 以鲤(Cyprinus carpio L.)为受试对象,探究3种不同烷基链咪唑类离子液体(Alkyl ionic liquids,AILs)([C6mim]Cl、[C8mim]Cl和[C_(12)mim]Cl,暴露浓度分别为4.97、2.15和0.09 mg/L)对鲤肠道的毒性作用。结果表明随烷基链长度增加,与对照组(NC)相比(1)鲤肠绒毛高度和肌层厚度显著降低(P<0.05);(2)[C8mim]Cl组T-AOC水平显著性升高(P<0.05);[C_(12)mim]Cl组GSH-Px、LDH、ACP酶活性和T-AOC水平显著性升高(P<0.05);(3)促炎因子IL-6、IL-8基因表达量显著升高(P<0.05),[C_(12)mim]Cl组尤为明显;(4)[C6mim]Cl和[C_(12)mim]Cl组鲤肠道菌群Chao1和Ace指数显著升高(P<0.05)。PCoA和UPGMA聚类分析结果表明3种AILs暴露改变了鲤肠道菌群组成。[C8mim]Cl和[C_(12)mim]Cl组有益菌厚壁菌门(Firmicutes)丰度显著降低(P<0.05)。3种AILs暴露均使黄杆菌属(Flavobacterium)等致病菌丰度显著增加(P<0.05),鲸杆菌属(Cetobacterium)等有益菌丰度降低。PICRUSt2菌群功能预测分析发现,[C6mim]Cl、[C_(12)mim]Cl组辅助因子和维生素代谢、外源生物降解和代谢等与碳氮循环功能相关途径丰度升高。综上,不同烷基链AILs暴露均可造成鲤肠道组织形态改变、氧化应激和免疫损伤,改变肠道菌群结构,且毒性随烷基链长度增加呈一定上升趋势。研究结果将为不同烷基链AILs对鱼类肠道的生态毒性机制研究提供一定的理论依据。 展开更多
关键词 咪唑类离子液体 氧化应激 免疫 肠道菌群 黄河鲤
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嗜黏蛋白阿克曼菌对鲤糖代谢的调控机制
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作者 王亚娓 黄真屹 +6 位作者 杨博雅 游富 张新党 杨国坤 常绪路 冯世坤 孟晓林 《水产学报》 CAS CSCD 北大核心 2024年第2期44-59,共16页
为探究巴氏灭活的阿克曼菌(Akk)调控鲤糖代谢的分子机制,实验通过不同糖水平(20%、30%、40%、50%葡萄糖)探究体重(10.5±1.0)g的鲤4周和8周时胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)分泌及阿克曼菌定植的时空变化;在不同... 为探究巴氏灭活的阿克曼菌(Akk)调控鲤糖代谢的分子机制,实验通过不同糖水平(20%、30%、40%、50%葡萄糖)探究体重(10.5±1.0)g的鲤4周和8周时胰高血糖素样肽-1(glucagon-like peptide-1,GLP-1)分泌及阿克曼菌定植的时空变化;在不同糖水平实验基础上,设置含有40%葡萄糖的3个不同Akk浓度(10^(8)、10^(9)和10^(10)CFU/g)P-Akk组,探究添加P-Akk 4周后对鲤(16.38±0.39 g)糖代谢及GLP-1的调控;通过P-Akk孵育鲤原代肝脏、肠细胞实验,共同探究不同糖水平下鲤肠道Akk、GLP-1对糖代谢的调控机制。结果显示,(1)随着饲料中葡萄糖含量的升高,鲤血清中GLP-1b含量显著降低,4周时高糖组鲤肠道GLP-1a、GLP-1b以及肠道内容物中Akk丰度均显著降低,但8周时无显著性差异。(2)P-Akk处理后,鲤血清中葡萄糖、GLP-1含量显著减少;中肠绒毛高度、肌层厚度显著增加,GLP-1含量减少,短链脂肪酸含量增加,muc2 mRNA表达水平升高;此外,P-Akk上调肝脏中ampk、pi3k、akt及糖酵解基因gk、pfk mRNA表达量,下调糖异生基因pepck mRNA表达量。(3)原代肝脏、肠细胞孵育结果显示,P-Akk处理肠细胞后GLP-1含量显著减少,而gpr40的mRNA表达量升高;此外肝脏细胞中糖酵解基因gk、pfk mRNA表达量显著升高。综上,外源添加P-Akk可以缓解高糖饲料引起的血糖升高,维持葡萄糖稳态。该研究结果可为Akk作为益生菌在水产饲料中的应用提供理论基础。 展开更多
关键词 不同糖水平 胰高血糖素样肽-1(GLP-1) 糖代谢
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养殖池塘黄河鲤肠道硫酸盐还原菌群落特征解析
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作者 马丽美 蔡慧敏 +7 位作者 梁晓敏 游富 江爱霞 杨国坤 张新党 常绪路 张艳敏 孟晓林 《淡水渔业》 CSCD 北大核心 2022年第6期3-9,共7页
为探究鱼类肠道硫酸盐还原菌(sulfate-reduction bacteria,简称SRB)的群落特征,本研究选用黄河鲤(Cyprinus carpio L.)为研究对象,通过SRB功能基因(dsrB)高通量测序技术,对黄河鲤前、中和后肠SRB功能群进行检测。研究结果显示:黄河鲤肠... 为探究鱼类肠道硫酸盐还原菌(sulfate-reduction bacteria,简称SRB)的群落特征,本研究选用黄河鲤(Cyprinus carpio L.)为研究对象,通过SRB功能基因(dsrB)高通量测序技术,对黄河鲤前、中和后肠SRB功能群进行检测。研究结果显示:黄河鲤肠道SRB主要分布在中肠和后肠,中肠SRB主要包括变形菌门(Proteobacteria)的脱硫球茎菌属(Desulfobulbus)、脱硫弧菌属(Desulfovibrio)、互营杆菌属(Syntrophobacter),厚壁菌门(Firmicutes)的脱亚硫酸菌属(Desulfitobacterium)和绿弯菌门(Chloroflexi)的暖绳菌属(Caldilinea);后肠SRB主要分布在变形菌门的脱硫球茎菌属、脱硫弧菌属、脱硫菌属(Desulfotalea)、互营杆菌属、脱硫念珠菌属(Desulfomonile)和鞘氨醇单胞菌属(Sphingomonas)。依据高通量测序结果的FAPROTAX(Functional Annotation of Prokaryotic Taxa)数据库代谢功能预测显示,黄河鲤肠道SRB主要参与硫化合物呼吸、硫酸盐呼吸和化能异养功能。 展开更多
关键词 黄河鲤(Cyprinus carpio L.) 肠道菌群 硫酸盐还原菌(SRB) dsrB高通量 功能预测
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Associations between Health-Related Fitness and Cardio-Metabolic Blood Profiles in Low-Income Children
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作者 Ryan D. Burns Timothy A. Brusseau +1 位作者 you fu James C. Hannon 《Open Journal of Preventive Medicine》 2015年第9期370-376,共7页
Children from low-income families have a higher incidence for developing early onset cardio-metabolic disease risk factors. Optimal levels of health-related fitness may attenuate risk, but little research has examined... Children from low-income families have a higher incidence for developing early onset cardio-metabolic disease risk factors. Optimal levels of health-related fitness may attenuate risk, but little research has examined its relationships with individual cardio-metabolic blood markers in low-income children. The purpose of this study was to examine the prevalence and correlates of unfavorable cardio-metabolic blood profiles in children from low-income families. Data were collected and analyzed on 124 children (mean age = 10.4 ± 0.9 years;57 girls, 67 boys;97% Hispanic) recruited from three urban Title I schools from the state of Utah in the US Health-related fitness. Measures were collected using the validated FITNESSGRAM fitness test battery. The Cholestech LDX system was used to analyze students’ total cholesterol (TC), LDL cholesterol, HDL cholesterol, triglycerides (TRI), and blood glucose (BG). Capillary blood samples via finger sticks were collected while each student was in a fasted state before school hours. Unfavorable measurements were defined as TC ≥ 200 mg/dL, LDL ≥ 130 mg/dL, HDL 150 mg/dL, and BG ≥ 100 mg/dL (pre-diabetes). Approximately 5.3% of the total sample had unfavorable TC, 16.7% had unfavorable HDL, 16.0% had unfavorable LDL, 15.2% had unfavorable TRI, and 25.4% had unfavorable BG (pre-diabetes). Pearson’s chi-square tests revealed no significant differences between sexes on any unfavorable classification after alpha level adjustment (p > 0.01). When all parameters were analyzed as continuous variables, Spearman’s rank correlation revealed a statistically significant linear relationship between aerobic fitness and LDL in boys (rs = -?0.65, p - 0.46, p < 0.01), and between BMI and BG in girls (rs = 0.56, p < 0.01). Aerobic fitness relates to LDL cholesterol in low-income boys and BMI relates to HDL cholesterol and BG in low-income girls. 展开更多
关键词 AEROBIC FITNESS Blood Glucose Body Mass Index CHOLESTEROL
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Association of Adolescent Bullying Victimization with Meeting 24-hour Movement Behavior Recommendations:A Cross-Sectional Study Using the Combined 2015-2019 Youth Risk Behavior Survey
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作者 you fu Ryan DBurns +2 位作者 Peng Zhang Samantha Dyer Nora Constantino 《Journal of Science in Sport and Exercise》 CSCD 2023年第2期182-190,共9页
Purpose To examine the association between bullying victimization and meeting recommendations for the 24-h movement behaviors of physical activity,screen use,and sleep in adolescents.Methods Participants were a sample... Purpose To examine the association between bullying victimization and meeting recommendations for the 24-h movement behaviors of physical activity,screen use,and sleep in adolescents.Methods Participants were a sample of US adolescents from the 2015–2019 national Youth Risk Behavior Survey(N=43,847;Mean age=16.0±1.2 years;50.7%female).The bullying victimization variables included the bullying expe-riences at school and online.Weighted multivariable logistic regressions analyzed associations between bullying victimiza-tion and meeting recommendations for 24-h movement behaviors controlling for age,sex,race/ethnicity,and weight status.Results Adolescents meeting non-academic screen and sleep recommendations had 25%(OR=0.75,95%CI 0.69–0.82)and 19%(OR=0.81,95%CI 0.74–0.89)lower odds to be bullied at school.Adolescents meeting non-academic screen recommen-dation had 27%lower odds(OR=0.73,95%CI 0.58–0.91)of experience online bullying.Male adolescents who met physical activity,non-academic screen use,and sleep recommendations had lower odds of experience the bullying at school of 29%(OR=0.71,95%CI 0.59–0.85),26%(OR=0.74,95%CI 0.61–0.90),and 31%(OR=0.69,95%CI 0.55–0.86),respectively.Among the females,non-academic screen use was the only movement behavior yielded statistical significance,in that those who met the recommendation had 15%(OR=0.85,95%CI 0.73–0.98)lower odds of experience the bullying at school.Conclusion The presence of meeting 24-h movement behavior recommendations associates with a lower probability of bullying at school and online in adolescents.Interestingly,these associations tended to be stronger and more consistent in males compared to females. 展开更多
关键词 School bullying Cyber bullying YOUTH Behavior
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Cavitation performance of high-speed centrifugal pump with annular jet and inducer at different temperatures and void fractions 被引量:8
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作者 Jin Jiang Yan-hui Li +4 位作者 Chong-yan Pei Lin-lin Li you fu Hong-gui Cheng Qiang-qiang Sun 《Journal of Hydrodynamics》 SCIE EI CSCD 2019年第1期93-101,共9页
The cavitation is very common in a centrifugal pump,especially when the speed is very high,and it seriously influences the centrifugal pump performance.In this investigation,the RNG k-ε turbulence model and the cavit... The cavitation is very common in a centrifugal pump,especially when the speed is very high,and it seriously influences the centrifugal pump performance.In this investigation,the RNG k-ε turbulence model and the cavitation model with consideration of the mass transferring are first used to simulate the cavitation performance of the high-speed centrifugal pump without taking any measure for improving the pump cavitation performance.The calculation results reveal that a number of bubbles appear in the centrifugal pump flow channel,and the head as well as the flow rate of the high-speed centrifugal pump are far from its design condition.The cavitation performance can be improved effectively by arranging a variable pitch inducer and adopting an annular nozzle scheme.The flow field analysis of the pump is conducted to obtain the suitable working temperature distribution at different void fractions.On one hand,with the same void fraction,the head of the centrifugal pump drops slowly with the increase of temperature.However,when the temperature exceeds 90°C,the head of the pump drops rapidly.On the other hand,at the constant temperature,the higher the void fraction,the worse the cavitation performance.This research conducted under different temperatures and void fractions provides some guidance for designing an effective high-speed centrifugal pump. 展开更多
关键词 High-speed CENTRIFUGAL pump CAVITATION performance INDUCER ANNULAR NOZZLE VOID fraction
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Comparisons of Subjective and Objective Measures of Free-Living Daily Physical Activity and Sedentary Behavior in College Students
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作者 Ya-Wen Hsu Chia-Chang Liu +3 位作者 Yen-Jung Chang Yi-Ju Tsai Wan-Chi Tsai you fu 《Journal of Science in Sport and Exercise》 2021年第2期186-194,共9页
Purpose To compare physical activity and sedentary behavior between four commonly used subjective and objective meas-ures:the 7-day Physical Activity Recall (7DPAR),International Physical Activity Questionnaire (IPAQ)... Purpose To compare physical activity and sedentary behavior between four commonly used subjective and objective meas-ures:the 7-day Physical Activity Recall (7DPAR),International Physical Activity Questionnaire (IPAQ),pedometer,and accelerometer.Methods A total of 130 college students completed four measures for the same 7 days.Body composition was measured using a bioelectric impedance analyzer.Wilcoxon signed rank tests and Spearman correlations were performed to compare estimates between activity measures.The Spearman correlations between different activity measures were further examined separately for the higher and the lower body fat groups.Results Compared with accelerometer-derived data,both the 7DPAR and the IPAQ overestimated light physical activity(P < 0.001) while underestimated sedentary behavior (P < 0.001).Across comparisons,the highest correlation was found between accelerometers and pedometers on steps/day (r =0.72,P < 0.001).The 7DPAR and the IPAQ were correlated with each other for all physical activity variables and sedentary behavior (r =0.37-0.45).There were low correlations (r=0.20-0.47) between the 7DPAR,the IPAQ,and accelerometers in sedentary behavior,light physical activity,and vigorous physical activity.Higher correlations between different activity modalities were observed among individuals with lower body fat(r=0.41-0.80) than among those with higher body fat (r=0.31-0.65).Conclusions The 7DPAR and the IPAQ yielded comparable estimation of moderate physical activity relative to accelerom-eters.There were significant differences in sedentary time across activity measures.Body compositions should be considered when comparing the estimates of activity levels between subjective and objective instruments. 展开更多
关键词 ACCELEROMETER PEDOMETER SELF-REPORT Body fat College student
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Effect of the Resistance Exercise on Elementary School Students’Physical Fitness
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作者 you fu Ryan D.Burns +2 位作者 Nora Constantino Jim Fitzsimmons Peng Zhang 《Journal of Science in Sport and Exercise》 2019年第2期184-191,共8页
Background Physical activity programs using components of resistance have the potential to improve health in school chil-dren.The purpose of this study was to examine the effect of a school-based resistance exercise p... Background Physical activity programs using components of resistance have the potential to improve health in school chil-dren.The purpose of this study was to examine the effect of a school-based resistance exercise program on physical fitness in elementary school students.Methods The sample was 256 children(mean age=8.3±2.5 years;119 girls)from kindergarten to 5th grade.Participants performed a 10-min resistance exercise 2-3 times in each school day.Physical fitness outcomes were assessed using President’s Physical Fitness Challenge test.Measures were collected at baseline and at a 6-month post-test time point.A 2×2 doubly MANOVA was employed to examine the effect of sex and time.Results The multivariate model was statistically significant with a main time effect(Wilks’λ=0.19,F=290.9,P<0.001).Follow-univariate tests found significant differences between time-points on flex arm hang(P=0.033),shuttle run(P<0.001),and 1-mile run/walk times(P<0.001).Conclusions A 6-month resistance exercise program improved upper body strength and cardiorespiratory endurance in elementary school aged children.The use of resistance exercise intervention during school day can be effectively used to promote physical fitness and ultimately improve the health of children. 展开更多
关键词 Resistance exercise CHILDREN Physical fitness
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