Efficacy and safety of a novel multi-electrode radiofrequency ablation catheter for renal sympathetic denervation in pigs

ObjectiveTo 调查一根自我开发的新奇多电极 radiofrequency 脱离导管(火花) 的安全和功效因为 14 头试验性的缩小的猪全部的基于导管的肾的 denervation (RDN ).MethodsA 随机被划分成四个组(55 &#x000b0； &#x00026；5 瓦特， 55 &#x000b0； &#x00026；8 瓦特， 65 &#x000b0； &#x00026；5 瓦特，和 65 &#x000b0；&#x00026；8 瓦特组) 。火花被用于左和恰好肾的动脉 radiofrequency 脱离。象肾的动脉X线摄影法一样从肾的动脉和静脉收集的血样品以前在所有动物上被执行，立即在以后，并且在过程到以后的三个月评估血浆高血压蛋白原酶的火花 on the levels 的效果，醛固酮，血管收缩素我，并且象肾的 arteries.ResultsOne 猪的病理学的变化一样的血管收缩素 II 死于一个麻药事故， 13 头猪成功地经历了双边的肾的动脉脱离。与基本大小相比，在所有四个组的猪显著地减少了在过程以后的吝啬的动脉的压力。组织病理学说的分析证明这个过程能导致内层的增生，在象在 perineurium 的煽动性的细胞渗入和纤维变性那样的肾的动脉的重要外部同情的神经损坏，神经纤维的不平的分发，织物坏死，严重 vacuolization ，碎裂并且不清楚的 nucleoli 髓磷脂退化，稀少的轴突，并且连续性的打断。另外，肾的动脉 radiofrequency 脱离能显著地减少血浆高血压蛋白原酶的层次，醛固酮，血管收缩素我，和在 pigs.ConclusionsThe 结果的血管收缩素 II 建议那基于导管的 radiofrequency 脱离能有效地移开的这类多电极外部肾的同情的神经和还原剂在猪的全身的高血压蛋白原酶血管收缩素系统的活动，因此在猪便于全身的血压的控制。

Infection density pattern of Cardinium affects the responses of bacterial communities in an invasive whitefly under heat conditions

Communities of bacteria,especially symbionts,are vital for the growth and development of insects and other arthropods,including Bemisia tabaci Mediterranean(MED),a destructive and invasive insect pest.However,the infection density patterns and influence factors of bacteria in whiteflies,which mainly include symbionts,remain largely unclear.To reveal the different density patterns of Cardinium in B.tabaci MED popula-tions and the impacts of high temperatures on whiteflies with different Cardinium density infection patterns,2 isofemale lines isolated from B.tabaci MED from the same geo-graphical population of China and from B.tabaci MED collected from other countries and locations were examined using several techniques and methods,including fluorescence in situ hybridization(FISH),quantitative real-time polymerase chain reaction(qPCR),16S rRNA gene sequencing,and 2b-RAD sequencing.The results showed that there were 2 different infection density patterns of Cardinium in B.tabaci MED(including 1 high-density pattern and 1 low-density pattern).For whiteflies with low-density Cardinium,conventional PCR could not detect Cardinium,but the other techniques confirmed that there was a low level of Cardinium within hosts.High temperature significantly decreased the diversity of bacterial communities:the relative titer of Cardinium increased but the density of Rickettsia decreased in the isofemale line with high-density Cardinium.How-ever,high temperature did not influence the diversity and symbiont density in the line with low-density Cardinium.Moreover,high temperature influenced the functions of bacterial communities in whiteflies with high-density Cardinium but did not affect the bacterial functions in whiteflies with low-density Cardinium.Our results provide novel insights into the complex associations between symbionts and host insects.

基于卷积神经网络的微带滤波器结构参数估计

针对神经网络微波建模耗时难的问题,提出了一种基于卷积神经网络的微带滤波器结构参数估计方法。采用单开路短截线加载矩形环谐振器和横向信号干扰技术分析和设计了一款双频带、三频带和四频带的微带滤波器,并利用电磁仿真软件提取数据。将微带滤波器的S参数和结构参数分别作为模型的输入和输出进行训练,并通过训练好的模型进行结构参数预测。利用卷积神经网络设计微带滤波器的方法,能够有效解决目前基于神经网络设计微带滤波器时输入参数较多、全连接造成模型复杂以及耗时长等问题。仿真结果表明,该方法设计的微带滤波器S参数有较高的准确率。

Genome-wide analysis of odorant-binding proteins and chemosensory proteins in the sweet potato whitefly, Bemisia tabaci

Odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) of insects are thought to play roles in olfactory recognition affecting host choice, copulation, reproduction and other behaviors. Previous descriptions of OBPs and CSPs in the whitefly Bemisia tabaci often provided no or incomplete genetic information. In this study, we present a genome-wide and transcriptome-wide investigation of the OBPs and CSPs in B. tabaci MEAMl (Middle East-Asia Minorl species). Eight OBP and 19 CSP genes were identified that covered all previous sequences. Phylogenetic analyses showed that the CSP genes had a lineage-specific expansion (BtabBCSP人 BtabBCSP3, BtabBCSPl3, BtabBCSPl 7, BtabBCSP18 and BtabBCSP 19). Expression profiling of OBPs and CSPs by transcriptome sequencing and quantitative real-time polymerase chain reaction (qPCR) revealed that expression patterns differed among developmental stages of B. tabaci MEAM 1. Five OBP genes and 11 CSP genes significantly differed between males and females;four of the 19 CSP genes were highly expressed in adults, while two were highly expressed in nymphs. The expression profiles of the OBP and CSP genes in different tissues of B. tabaci MEAM1 adults were analyzed by qPCR. Four OBP genes found in B. tabaci MEAM 1 were highly expressed in the head. Conversely, only two CSPs were enriched in the head, while the other six CSPs were specifically expressed in other tissues. Our results provide a foundation for future research on OBPs and CSPs in B. tabaci.

Effects of host, temperature and relative humidity on competitive displacement of two invasive Bemisia tabaci biotypes [Q and B]

Bemisia tabaci shifted unexpectedly in China from a predominance of B biotype to Q biotype during 2005-2008. This observation stimulated an interest in inves- tigating whether environmental factors, including host, temperature and relative humidity （RH） could possibly explain the observed shift in biotypes distribution. Results indicated that all three parameters examined influenced biotype survivability. The percentage of B biotype, when reared together on pepper plants with the Q biotype, decreased significantly from 66.7% in the founder population, to 13.6% and 3.7% in the first and second genera- tions, respectively. When the B （founder at 66.7%） and Q （founder at 33.3%） biotypes were reared together on eggplant alone, or on pepper-plus-eggplant combination, the population size of the B biotype either remained constant, or increased somewhat in the first and sec- ond generations. On eggplant, the effects of RH and temperature on the competitiveness between the Q and B biotypes （3 pairs of Q and 6 pairs of B） were not significant.

Management of diamondback moth, Plutella xylostella （Lepidoptera： Plutellidae） by mating disruption

Field trials were conducted in China in 2008 and 2009 to evaluate the efficacy of mating disruption （MD） on diamondback moth, Plutella xylostella, in cabbage, Brassica oleracea var. capitata. Effectiveness was positively correlated with the MD dispenser density in the field. A density of 167 MD dispensers per ha produced an average population decrease of about 50% compared to the conventional-practice field. Significant fewer males were captured in pheromone-treated and conventional-practice fields than in the blank control field, but the difference was not significant between the pheromone-treated and conventional-practice fields. In addition, fewer eggs and larvae were observed in pheromone-treated fields. Our results suggest mating disruption coupled with minimal insecticidal supplements is a promising solution for resistance management and control of diamondback moth infestation.

Oral delivery of dsHvlwr is a feasible method for managing the pest Henosepilachna vigintioctopunctata(Coleoptera:Coccinellidae)

RNA interference(RNAi)techniques have emerged as powerful tools that facilitate development of novel management strategies for insect pests,such as Henosepilachna vigintioctopunctata(Coleoptera:Coccinellidae),which is a major pest of solanaceous plants in Asia.In this study,the potential of oral delivery of in vvYro-synthesized and bacterially expressed double-stranded H.vigintioctopunctata lesswright(Iwr)gene(dsHvlwr)to manage of H.vigintioctopunctata was investigated.Our results showed that the gene Hvlwr had a 480-bp open reading frame and encoded a 160-amino acid protein.Hvlwr expression levels were greater in the fat body than other tissue types.Hvlwr silencing led to greater H.vigintioctopunctata mortality rates and appeared to be time-and partially dose-dependent,likely as a result of the number of hemocytes increasing with dsRNA concentration,but decreasing with time.Bacterially expressed dsHvlwr that was applied to leaf discs caused 88%,66%,and 36%mortality in 1st instars,3rd instars,and adults after 10,10,and 14 d,respectively;when applied to living plants,there was greater mortality in 1 st and 3rd instars,but there was no effect on adults.Furthermore,dsHvlwr led to improved plant protection against H.vigintioctopunctata.Our study shows an effective dietary RNAi response in H.vigintioctopunctata and that Hvlwr is a promising RNAi target gene for control of this pest species.

Genome-wide identification and analysis of sulfatase and sulfatase modifying factor genes in Bemisia tabaci (Hemiptera: Aleyrodidae)

The invasive pest whitefly(Bemisia tabaci)is a complex species,of which Middle East-Minor Asia 1(MEAM1)and Mediterranean(MED)are the two most damaging members.Previous research showed that cabbage is frequently infested with MEAM1 but seldomly with MED,and this difference in performance is associated with glucosi-nolate(GS)content.Some insects can modify GS using glucosinolate sulfatase(SULF),the activity of which is regulated by sulfatase modifying factor 1(SUMF1);therefore,to increase our understanding of different performances of MEAM1 and MED on cabbage plants,we identified and compared nine putative SULFs and one SUMF in MEAM1 and MED.We found that the lengths of two genes,BtSulf2 and BtSulf4,differed between MEAM1 and MED.The messenger RNA levels of BtSulf4 increased more than 20-fold after MEAMl and MED adults were exposed to GS,but BtSulJ2 expression was only induced by GS in MEAM1.Knockdown of BtSulf2 and BtSulf4 in MEAM1 resulted in a substantial increase in the mortality of GS-treated adults but not in MED.These results indicate that differences in BtSulJ2 and BtSulf4 sequences and/or expression may explain why MEAM1 performs better than MED on cabbage.Our results provide a basis for future functional research on SULF and SUMF in B.tabaci.

Feeding behavior explains the different effects of cabbage on MEAM1 and MED cryptic species of Bemisia tabaci

MEAMI(Middle East-Asia Minor 1."B"biotype)anpl MED(Mediterranean,"Q"biotype)are the two most destructive ery pie species of the Bemisia tabaci complex on the planet.Our previous studies have shown that MEAMI outcompets MED on cabbage;the underlying mechanism is unknown.In the Brassicaceae family,the glucosinolate-myrosinase defense system plays a crucial role im deterring feeding,inhibiting growth,and eausing acute toxicity against a wide range of generalist herbivores.In the present study.we first compared the surv ival of MEAMI and MED exposed to sinigin(a glucosinolate)and myosinase(an enzyme that degrades glucosinolates);we found that survival of both species was high in response 10 sinigrin alone bul was near zer0 in response to sinigrin+myrosinase.We then used electropenetrography(lectrical penetration graphs,EPG)to assess the feeding bchaviors of MEAMI and MED whiteflies on cabbage.The EPG results revealed that the me an dunution of each potential drop(pd,indicating an intreellular puncture)was subsantialy longer for MED than MEAMI on eabbage,indicating that the exposure to the toxic hydrolysates of glucosinolate and myrosinase is greater for MED than for MEAMI.We therefore conclude that dffreces in penetrating bchaviors may help explain the dfferent fiects of cabbuge on MEAMI and MED whitefly species.

Effects of abamectin selection on the genetic differentiation within Plutella xylostella （Lepidoptera： Plutellidae） based on amplified fragment length polymorphism

Diamondback moth, Plutella xylostella （L.） is one of the most serious insect pests for its remarkable ability to develop resistance to virtually every insecticide that has been used against it. In the present study, amplified fragment length poly- morphism （AFLP） is used to study the genetic differentiation as well as the effects of abamectin-selection on population genetic differentiation for P. xylostella. A dendrogram was constructed from the matrix of genetic distances using the STATISTICA software （Version 4.5） and unweighted pair-group method with arithmetic averages （UPGMA）. The data demonstrated that compared to the susceptible strain （ABM-s）, the heterozy- gosity in the abamectin-resistant strain （ABM-r） decreased with the increased selection pressure and resistant level. When the resistance ratio was below 4.3, there was no sig- nificant differentiation at the genome DNA level. When the resistance ratio reached 5.8, a fairly significant differentiation began to appear, and when the resistance ratio reached and exceeded 8.1, there appeared a significant genetic differentiation. The results suggest that abamectin selection is associated with increased genetic polymorphism in P. xylostella.