Foreword Patent issues may be existed in this document, and the Word Federation of Chinese Medicine Societies declared that they are not to blame.The main drafting Committee of this docement:First People’s Hospital A...Foreword Patent issues may be existed in this document, and the Word Federation of Chinese Medicine Societies declared that they are not to blame.The main drafting Committee of this docement:First People’s Hospital Affiliated to Shanghai Jiaotong University, Eye hospital,China academy of Chinese Medical Sciences, Beijing Tongren Hospital Affiliated to Capital Medical University, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, National Eye Institute/National Institute of Health.展开更多
Objective:The objective of this study is to investigate the inhibitory effect of LXHY,a Chinese medicine compound formula,on choroidal neovascularization(CNV) and to find the possible working mechanism.Methods:CNV was...Objective:The objective of this study is to investigate the inhibitory effect of LXHY,a Chinese medicine compound formula,on choroidal neovascularization(CNV) and to find the possible working mechanism.Methods:CNV was induced in C57 BL/6 mice by krypton laser and bone marrow-derived cells(BMCs) isolated from enhanced green fluorescent protein(EGFP) transgenic mice were injected through tail vein 0.5–1 h after the laser surgery.The BMC-treated mice were randomly divided into two groups gavaged with either distilled water(DW group) or LXHY formula solution from day 1 after laser surgery.On days 7,14,and 28 after treatment,histopathologic examination,fundus fluorescein angiography,and choroidal flatmount assay were performed to measure the CNV severity and BMC recruitment.CXCR4 levels in peripheral blood were measured by enzyme-linked immunosorbent assay.Stromal cell-derived factor-1α(SDF?1α),vascular cell adhesion molecule-1(VCAM-1),and intercellular adhesion molecule-1(ICAM-1) were detected by immunofluorescent staining.Results:On days 7 and 14 after treatment,CNV lesions in the LXHY-treated mice showed less recruitment of BMCs and were smaller in size compared to DW-treated mice.Histological examination also confirmed less severe CNV lesions in the LXHY group.CXCR4 levels in peripheral blood in the LXHY group were less than that of DW group on days 7 and 14.Moreover,the expression levels of SDF-1α,ICAM?1,and VCAM?1 at the lesion sites in the LXHY group were lower compared with the DW group.Conclusion:This experiment indicated that LXHY formula could inhibit CNV formation and development,probably by inhibiting the recruitment and attachment of BMCs into CNV area.展开更多
基金supported by funds from the National Natural Science Foundation of China(81973912)Special Project of International Cooperation in TCM of the State Administration of Traditional Chinese Medicine(0610-2040NF020931)+1 种基金the China Center for Evidence-Based Traditional Chinese Medicine(2020YJSZX-2)China Academy of Chinese Medical Sciences Innovation Fund(CI2021A00701-3)。
文摘Foreword Patent issues may be existed in this document, and the Word Federation of Chinese Medicine Societies declared that they are not to blame.The main drafting Committee of this docement:First People’s Hospital Affiliated to Shanghai Jiaotong University, Eye hospital,China academy of Chinese Medical Sciences, Beijing Tongren Hospital Affiliated to Capital Medical University, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, National Eye Institute/National Institute of Health.
基金financially supported by grants from the National Natural Science Foundation of China(No.81072846,81674033,81102618)TCM "Belt and Road Initiative" Cooperative project funded by China Academy of Chinese Medical Sciences(GH2017-04-02)the Scientific Research Foundation for the Returned Overseas Chinese Scholars,Ministry of Human Resources and Social Security of the People’s Republic of China(2010)
文摘Objective:The objective of this study is to investigate the inhibitory effect of LXHY,a Chinese medicine compound formula,on choroidal neovascularization(CNV) and to find the possible working mechanism.Methods:CNV was induced in C57 BL/6 mice by krypton laser and bone marrow-derived cells(BMCs) isolated from enhanced green fluorescent protein(EGFP) transgenic mice were injected through tail vein 0.5–1 h after the laser surgery.The BMC-treated mice were randomly divided into two groups gavaged with either distilled water(DW group) or LXHY formula solution from day 1 after laser surgery.On days 7,14,and 28 after treatment,histopathologic examination,fundus fluorescein angiography,and choroidal flatmount assay were performed to measure the CNV severity and BMC recruitment.CXCR4 levels in peripheral blood were measured by enzyme-linked immunosorbent assay.Stromal cell-derived factor-1α(SDF?1α),vascular cell adhesion molecule-1(VCAM-1),and intercellular adhesion molecule-1(ICAM-1) were detected by immunofluorescent staining.Results:On days 7 and 14 after treatment,CNV lesions in the LXHY-treated mice showed less recruitment of BMCs and were smaller in size compared to DW-treated mice.Histological examination also confirmed less severe CNV lesions in the LXHY group.CXCR4 levels in peripheral blood in the LXHY group were less than that of DW group on days 7 and 14.Moreover,the expression levels of SDF-1α,ICAM?1,and VCAM?1 at the lesion sites in the LXHY group were lower compared with the DW group.Conclusion:This experiment indicated that LXHY formula could inhibit CNV formation and development,probably by inhibiting the recruitment and attachment of BMCs into CNV area.
