[ Objective ] The paper was to screen the better extraction method for herbicidal active substances of Eupatorium adenophorum. [ Method ] Three com- mon extraction methods, Soxhlet extraction, ultrasonic extraction an...[ Objective ] The paper was to screen the better extraction method for herbicidal active substances of Eupatorium adenophorum. [ Method ] Three com- mon extraction methods, Soxhlet extraction, ultrasonic extraction and conventional extraction method, were used to prepare E. adenophorum extracts, and the herblcidal activity of E. adenophorum extracts extracted by 3 methods was studied by seed germination method. [ Result] The extraction rate of Soxhlet extraction was the highest of （22.54 ± 0.48 ）%, and the herbicidal activities of E. adenophorum extracted by Soxhlet extraction was relatively stronger. [ Conclusion] Soxhlet extraction was the better extraction method for herbicidal active substances of E. adenophorum.展开更多
For mistakes taken in pesticide bioassays, teaching experimental design is improved in the paper, so as to let students explore and analyze in teaching experiments to get a deeper understanding of theoretical knowledg...For mistakes taken in pesticide bioassays, teaching experimental design is improved in the paper, so as to let students explore and analyze in teaching experiments to get a deeper understanding of theoretical knowledge, thereby effectively avoiding frequently-taken mistakes in pesticide bioassays.展开更多
[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment fr...[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment friendship. [Method] Using mung bean rooting method, the rooting promotion effects of 33 plant extracts on mung bean section were studied. [Result] When the concentration was 1 mg/mL, Eupatorium adenophorum （stem and leaf）, Cryptomeriafortunei （stem and leaf）, Pinus yunnanensis （stem）, Pinus yunnanensis （leaf）, Rhododendron simsii （leaf）, Pinus armandii （leaf）, Litchi chinensis （pericarp）, P/nus armandiifruit （fruit） and Alnus cremastogyne （leaf） showed strong promotion effect on rooting of mung bean sections, and the rooting promotion rate reached over 100% ; the roo- ting promotion effect ofE. adenophorum （stem and leaf） was the strongest, reaching（1068.42 ＋7.87）%, which was extremely higher than the extracts of other plants. [Conclusion] E. adenophorum （stem and leaf）, C. fortunei （stem and leaf）, P. yunnanens/s （stem）, P. yurmanens/s （leaf）, R. simsii （leaf）, P. armandii （leaf）, L. chinensis （pericarp）, P. armandiifruit （fruit） and A. cremastogyne （leaf） all contained substances with st/ong rooting promotion effect, which could be further used for development of natural rooting reagent.展开更多
基金Supported by Youth Fund Project of Yunnan Agricultural University
文摘[ Objective ] The paper was to screen the better extraction method for herbicidal active substances of Eupatorium adenophorum. [ Method ] Three com- mon extraction methods, Soxhlet extraction, ultrasonic extraction and conventional extraction method, were used to prepare E. adenophorum extracts, and the herblcidal activity of E. adenophorum extracts extracted by 3 methods was studied by seed germination method. [ Result] The extraction rate of Soxhlet extraction was the highest of （22.54 ± 0.48 ）%, and the herbicidal activities of E. adenophorum extracted by Soxhlet extraction was relatively stronger. [ Conclusion] Soxhlet extraction was the better extraction method for herbicidal active substances of E. adenophorum.
基金Supported by Construction Project of " National Teaching Team of Plant Protection" in 2008Construction Project of National " Plant Protection Specialty"( TS11138)Provincial Excellent Course Construction Project of Pesticide Science in Yunnan Province
文摘For mistakes taken in pesticide bioassays, teaching experimental design is improved in the paper, so as to let students explore and analyze in teaching experiments to get a deeper understanding of theoretical knowledge, thereby effectively avoiding frequently-taken mistakes in pesticide bioassays.
基金Supported by The Natural Science Foundation Project of Yunnan Province ( No. 2010CD056)
文摘[ Objective ] The paper was to study the rooting promotion activity of plant extracts, so as to lay the foundation for development of natural source of roo- fing reagent with high efficiency, safety and environment friendship. [Method] Using mung bean rooting method, the rooting promotion effects of 33 plant extracts on mung bean section were studied. [Result] When the concentration was 1 mg/mL, Eupatorium adenophorum （stem and leaf）, Cryptomeriafortunei （stem and leaf）, Pinus yunnanensis （stem）, Pinus yunnanensis （leaf）, Rhododendron simsii （leaf）, Pinus armandii （leaf）, Litchi chinensis （pericarp）, P/nus armandiifruit （fruit） and Alnus cremastogyne （leaf） showed strong promotion effect on rooting of mung bean sections, and the rooting promotion rate reached over 100% ; the roo- ting promotion effect ofE. adenophorum （stem and leaf） was the strongest, reaching（1068.42 ＋7.87）%, which was extremely higher than the extracts of other plants. [Conclusion] E. adenophorum （stem and leaf）, C. fortunei （stem and leaf）, P. yunnanens/s （stem）, P. yurmanens/s （leaf）, R. simsii （leaf）, P. armandii （leaf）, L. chinensis （pericarp）, P. armandiifruit （fruit） and A. cremastogyne （leaf） all contained substances with st/ong rooting promotion effect, which could be further used for development of natural rooting reagent.
