In this study,we developed a novel on-line solid phase extraction(SPE)-ultra-high-performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)-based analytical method for simultaneously quantifying 12 illic...In this study,we developed a novel on-line solid phase extraction(SPE)-ultra-high-performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)-based analytical method for simultaneously quantifying 12 illicit drugs and metabolites(methamphetamine,amphetamine,morphine,codeine,6-monoacetylmorphine,benzoylecgonine,3,4-methylenedioxymethamphetamine,3,4-methylenedioxyamphetamine,cocaine,ketamine,norketamine,and methcathinone)and cotinine(COT)in wastewater samples.The analysis was performed by loading 2 m L of the sample onto an Oasis hydrophilic-lipophilic balance cartridge and using a cleanup step(5%methanol)to eliminate interference with a total run time of 13 min.The isotope-labeled internal standard method was used to quantify the target substances and correct for unavoidable losses and matrix effects during the on-line SPE process.Typical analytical characteristics used for method validation were sensitivity,linearity,precision,repeatability,recovery,and matrix effects.The limit of detection(LOD)and limit of quantification(LOQ)of each target were set at 0.20 ng/L and 0.50 ng/L,respectively.The linearity was between 0.5 ng/L and250 ng/L,except for that of COT.The intra-and inter-day precisions were<10.45%and 25.64%,respectively,and the relative recovery ranged from 83.74%to 162.26%.The method was used to analyze various wastewater samples from 33 cities in China,and the results were compared with the experimental results of identical samples analyzed using off-line SPE.The difference rate was between 19.91%and-20.44%,and the error range could be considered acceptable.These findings showed that on-line SPE is a suitable alternative to off-line SPE for the analysis of illicit drugs in samples.展开更多
[Objectives] The ecological environment of orchard has a direct impact on fruit quality,and straw mulching can effectively improve the physicochemical indicators of orchard soil,so as to achieve environmental protecti...[Objectives] The ecological environment of orchard has a direct impact on fruit quality,and straw mulching can effectively improve the physicochemical indicators of orchard soil,so as to achieve environmental protection and yield increase.[Methods]Tests were carried out for 3 consecutive years of mulching straw in vineyard of southern Jiangsu Province.[Results]The results showed that mulching straw continuously could effectively improve soil physicochemical properties,weed controlling effect,and the photosynthetic rate of leaves,as well as the yield and quality of grapes.The contents of total nitrogen,available phosphorus,quickacting potassium and organic matter increased up to 91.67%,259.76%,442.39% and 34.30%,respectively.Soil bulk density decreased by 7.15%,and the weed plant control effect reached 87.45%.The increased of fresh weight was up to 93.55%,and the increase of the leaf parenthetical rate was up to 18.24%.The increases of soluble solids content and single grain weight reached up to 8.82% and 12.75%.The rate of dehiscent fruit was decreased by 50.89%.The grape could pick for sale 3 days ahead,and the increase of unit area yield reached up to 13.16%.[Conclusions]Straw mulching cultivation in vineyard is a good model for efficient recycling of crop straw and safe production of quality grape,and it provides a new technical approach for straw utilization,promising with good economic and ecological benefits.展开更多
Studies have suggested that the nucleus accumbens(NAc)is implicated in the pathophysiology of major depression;however,the regulatory strategy that targets the NAc to achieve an exclusive and outstanding anti-depressi...Studies have suggested that the nucleus accumbens(NAc)is implicated in the pathophysiology of major depression;however,the regulatory strategy that targets the NAc to achieve an exclusive and outstanding anti-depression benefit has not been elucidated.Here,we identified a specific reduction of cyclic adenosine monophosphate(cAMP)in the subset of dopamine D1 receptor medium spiny neurons(D1-MSNs)in the NAc that promoted stress susceptibility,while the stimulation of cAMP production in NAc D1-MSNs efficiently rescued depression-like behaviors.Ketamine treatment enhanced cAMP both in D1-MSNs and dopamine D2 receptor medium spiny neurons(D2-MSNs)of depressed mice,however,the rapid antidepressant effect of ketamine solely depended on elevating cAMP in NAc D1-MSNs.We discovered that a higher dose of crocin markedly increased cAMP in the NAc and consistently relieved depression 24 h after oral administration,but not a lower dose.The fast onset property of crocin was verified through multicenter studies.Moreover,crocin specifically targeted at D1-MSN cAMP signaling in the NAc to relieve depression and had no effect on D2-MSN.These findings characterize a new strategy to achieve an exclusive and outstanding anti-depression benefit by elevating cAMP in D1-MSNs in the NAc,and provide a potential rapid antidepressant drug candidate,crocin.展开更多
The difficulty of reducing the diameter of lutetium oxide(Lu_(2)O_(3))continuous fibers below 50μm not only limits the flexibility of the sample but also seriously affects their application and development in high-en...The difficulty of reducing the diameter of lutetium oxide(Lu_(2)O_(3))continuous fibers below 50μm not only limits the flexibility of the sample but also seriously affects their application and development in high-energy lasers.In this work,a Lu-containing precursor with high ceramic yield was used as raw material,fiberized into precursor fibers by dry spinning.The pressure-assisted water vapor pretreatment(PAWVT)method was creatively proposed,and the effect of pretreatment temperature on the ceramization behavior of the precursor fibers was studied.By regulating the decomposition behavior of organic components in the precursor,the problem of fiber pulverization during heat treatment was effectively solved,and the Lu_(2)O_(3) continuous fibers with a diameter of 40μm were obtained.Compared with the current reported results,the diameter was reduced by about 50%,successfully breaking through the diameter limitation of Lu_(2)O_(3) continuous fibers.In addition,the tensile strength,elastic modulus,flexibility,and temperature resistance of Lu_(2)O_(3) continuous fibers were researched for the first time.The tensile strength and elastic modulus of Lu_(2)O_(3) continuous fibers were 373.23 MPa and 31.55 GPa,respectively.The as-obtained flexible Lu_(2)O_(3) continuous fibers with a limit radius of curvature of 3.5-4.5 mm had a temperature resistance of not lower than 1300℃,which established a solid foundation for the expansion of their application form in the field of high-energy lasers.展开更多
基金supported by the National Key Research and Development Program of China(Grant No.:2018YFC0807402)the National Natural Science Foundation of China(Grant No.:82073810)。
文摘In this study,we developed a novel on-line solid phase extraction(SPE)-ultra-high-performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)-based analytical method for simultaneously quantifying 12 illicit drugs and metabolites(methamphetamine,amphetamine,morphine,codeine,6-monoacetylmorphine,benzoylecgonine,3,4-methylenedioxymethamphetamine,3,4-methylenedioxyamphetamine,cocaine,ketamine,norketamine,and methcathinone)and cotinine(COT)in wastewater samples.The analysis was performed by loading 2 m L of the sample onto an Oasis hydrophilic-lipophilic balance cartridge and using a cleanup step(5%methanol)to eliminate interference with a total run time of 13 min.The isotope-labeled internal standard method was used to quantify the target substances and correct for unavoidable losses and matrix effects during the on-line SPE process.Typical analytical characteristics used for method validation were sensitivity,linearity,precision,repeatability,recovery,and matrix effects.The limit of detection(LOD)and limit of quantification(LOQ)of each target were set at 0.20 ng/L and 0.50 ng/L,respectively.The linearity was between 0.5 ng/L and250 ng/L,except for that of COT.The intra-and inter-day precisions were<10.45%and 25.64%,respectively,and the relative recovery ranged from 83.74%to 162.26%.The method was used to analyze various wastewater samples from 33 cities in China,and the results were compared with the experimental results of identical samples analyzed using off-line SPE.The difference rate was between 19.91%and-20.44%,and the error range could be considered acceptable.These findings showed that on-line SPE is a suitable alternative to off-line SPE for the analysis of illicit drugs in samples.
基金Supported by the Science and Technology Project of Changzhou City(CE20155037)
文摘[Objectives] The ecological environment of orchard has a direct impact on fruit quality,and straw mulching can effectively improve the physicochemical indicators of orchard soil,so as to achieve environmental protection and yield increase.[Methods]Tests were carried out for 3 consecutive years of mulching straw in vineyard of southern Jiangsu Province.[Results]The results showed that mulching straw continuously could effectively improve soil physicochemical properties,weed controlling effect,and the photosynthetic rate of leaves,as well as the yield and quality of grapes.The contents of total nitrogen,available phosphorus,quickacting potassium and organic matter increased up to 91.67%,259.76%,442.39% and 34.30%,respectively.Soil bulk density decreased by 7.15%,and the weed plant control effect reached 87.45%.The increased of fresh weight was up to 93.55%,and the increase of the leaf parenthetical rate was up to 18.24%.The increases of soluble solids content and single grain weight reached up to 8.82% and 12.75%.The rate of dehiscent fruit was decreased by 50.89%.The grape could pick for sale 3 days ahead,and the increase of unit area yield reached up to 13.16%.[Conclusions]Straw mulching cultivation in vineyard is a good model for efficient recycling of crop straw and safe production of quality grape,and it provides a new technical approach for straw utilization,promising with good economic and ecological benefits.
基金supported by National Natural Science Foundation of China(No.82104278)Leading Technology Foundation Research Project of Jiangsu Province(No.BK20192005,China)+4 种基金National Key Project of Science and Technology for Innovation Drugs of China(No.2017ZX09301013)CAMS Innovation Fund for Medical Sciences(CIFMS,No.2021-I2M-5-011,China)Sanming Project of Medicine in Shenzhen(No.SZSM201801060,China)Project of State Key Laboratory of Natural Medicines,China Pharmaceutical University(No.SKLNMKF202203,China)National Innovation and Entrepreneurship Training Program for Undergraduate,China Pharmaceutical University(Nos.2023103161381 and 2023103161287,China)。
文摘Studies have suggested that the nucleus accumbens(NAc)is implicated in the pathophysiology of major depression;however,the regulatory strategy that targets the NAc to achieve an exclusive and outstanding anti-depression benefit has not been elucidated.Here,we identified a specific reduction of cyclic adenosine monophosphate(cAMP)in the subset of dopamine D1 receptor medium spiny neurons(D1-MSNs)in the NAc that promoted stress susceptibility,while the stimulation of cAMP production in NAc D1-MSNs efficiently rescued depression-like behaviors.Ketamine treatment enhanced cAMP both in D1-MSNs and dopamine D2 receptor medium spiny neurons(D2-MSNs)of depressed mice,however,the rapid antidepressant effect of ketamine solely depended on elevating cAMP in NAc D1-MSNs.We discovered that a higher dose of crocin markedly increased cAMP in the NAc and consistently relieved depression 24 h after oral administration,but not a lower dose.The fast onset property of crocin was verified through multicenter studies.Moreover,crocin specifically targeted at D1-MSN cAMP signaling in the NAc to relieve depression and had no effect on D2-MSN.These findings characterize a new strategy to achieve an exclusive and outstanding anti-depression benefit by elevating cAMP in D1-MSNs in the NAc,and provide a potential rapid antidepressant drug candidate,crocin.
基金supported by the Key Program of the National Natural Science Foundation of China (No.52032003)the National Natural Science for Youth Foundation of China (Nos.52102093 and 52202090)+3 种基金the Shandong University Young Scholars Program (No.2016WLJH27)the Fundamental Research Funds for the Central Universities (No.2082019014)the China Postdoctoral Science Foundation (No.2021M690817)the Heilongjiang Provincial Postdoctoral Science Foundation (Nos.LBH-Z21050 and LBH-Z20144).
文摘The difficulty of reducing the diameter of lutetium oxide(Lu_(2)O_(3))continuous fibers below 50μm not only limits the flexibility of the sample but also seriously affects their application and development in high-energy lasers.In this work,a Lu-containing precursor with high ceramic yield was used as raw material,fiberized into precursor fibers by dry spinning.The pressure-assisted water vapor pretreatment(PAWVT)method was creatively proposed,and the effect of pretreatment temperature on the ceramization behavior of the precursor fibers was studied.By regulating the decomposition behavior of organic components in the precursor,the problem of fiber pulverization during heat treatment was effectively solved,and the Lu_(2)O_(3) continuous fibers with a diameter of 40μm were obtained.Compared with the current reported results,the diameter was reduced by about 50%,successfully breaking through the diameter limitation of Lu_(2)O_(3) continuous fibers.In addition,the tensile strength,elastic modulus,flexibility,and temperature resistance of Lu_(2)O_(3) continuous fibers were researched for the first time.The tensile strength and elastic modulus of Lu_(2)O_(3) continuous fibers were 373.23 MPa and 31.55 GPa,respectively.The as-obtained flexible Lu_(2)O_(3) continuous fibers with a limit radius of curvature of 3.5-4.5 mm had a temperature resistance of not lower than 1300℃,which established a solid foundation for the expansion of their application form in the field of high-energy lasers.