In this study,we developed a powder extruder system that can extrude and deposit powder mixtures to overcome the reported limitations of conventional dualpore scaffold manufacturing methods.To evaluate the extrusion a...In this study,we developed a powder extruder system that can extrude and deposit powder mixtures to overcome the reported limitations of conventional dualpore scaffold manufacturing methods.To evaluate the extrusion and deposition capability of the powder extruder system,3D tissue-engineering scaffolds with dual-pore characteristics were fabricated with a PCL/PEO/NaCl(polycaprolactone/polyethylene oxide/sodium chloride)powder mixture.In addition,to evaluate the fabricated scaffolds,their compressive modulus,morphology,and in-vitro cell activity were assessed.Consequently,it was confirmed that the proposed powder extruder system can fabricate dual-pore scaffolds with well-interconnected pores as well as arbitrary 3D shapes shown by the fabrication of a 3D femur-shape scaffold similar to the femur model.The results of the cell proliferation and Cell Counting Kit-8(CCK-8)assays,DNA content analysis and viability assays confirm that the dual-pore scaffold fabricated by the powder extruder system improves cell attachment,proliferation,and viability.展开更多
In this study, to fabricate dual-pore scaffolds with interconnected pores, Non-solvent Induced Phase Separation （NIPS） and Wire-Network Molding （WNM） techniques were combined. First, a mold with uniform slits was ...In this study, to fabricate dual-pore scaffolds with interconnected pores, Non-solvent Induced Phase Separation （NIPS） and Wire-Network Molding （WNM） techniques were combined. First, a mold with uniform slits was prepared, and needles were inserted into the mold. Subsequently, polycaprolactone （PCL） pellets were dissolved in tetrahydrofuran （THF） at a specified ratio. The slurry was mixed using hot plate stirrer at 1200 rpm for 24 hours at 40 ~C. The PCL slurry was subsequently injected into the mold. Thereafter, to exchange the THF （solvent） with the ethanol （non-solvent）, the mold was soaked in an ethanol bath. After removing the mold from the ethanol bath, the needles were removed from the mold. Consequently, dual-pore scaffold with interconnected pores was obtained. The surface morphology of the fabricated scaffolds were observed using Scanning Electron Microscope （SEM）. Moreover, cell culture experiments were performed using the CCK-8 assay, and the characteristics of cells grown on the dual-pore scaffolds were assessed and were compared with the NIPS-based 3D plotting scaffold.展开更多
文摘In this study,we developed a powder extruder system that can extrude and deposit powder mixtures to overcome the reported limitations of conventional dualpore scaffold manufacturing methods.To evaluate the extrusion and deposition capability of the powder extruder system,3D tissue-engineering scaffolds with dual-pore characteristics were fabricated with a PCL/PEO/NaCl(polycaprolactone/polyethylene oxide/sodium chloride)powder mixture.In addition,to evaluate the fabricated scaffolds,their compressive modulus,morphology,and in-vitro cell activity were assessed.Consequently,it was confirmed that the proposed powder extruder system can fabricate dual-pore scaffolds with well-interconnected pores as well as arbitrary 3D shapes shown by the fabrication of a 3D femur-shape scaffold similar to the femur model.The results of the cell proliferation and Cell Counting Kit-8(CCK-8)assays,DNA content analysis and viability assays confirm that the dual-pore scaffold fabricated by the powder extruder system improves cell attachment,proliferation,and viability.
文摘In this study, to fabricate dual-pore scaffolds with interconnected pores, Non-solvent Induced Phase Separation （NIPS） and Wire-Network Molding （WNM） techniques were combined. First, a mold with uniform slits was prepared, and needles were inserted into the mold. Subsequently, polycaprolactone （PCL） pellets were dissolved in tetrahydrofuran （THF） at a specified ratio. The slurry was mixed using hot plate stirrer at 1200 rpm for 24 hours at 40 ~C. The PCL slurry was subsequently injected into the mold. Thereafter, to exchange the THF （solvent） with the ethanol （non-solvent）, the mold was soaked in an ethanol bath. After removing the mold from the ethanol bath, the needles were removed from the mold. Consequently, dual-pore scaffold with interconnected pores was obtained. The surface morphology of the fabricated scaffolds were observed using Scanning Electron Microscope （SEM）. Moreover, cell culture experiments were performed using the CCK-8 assay, and the characteristics of cells grown on the dual-pore scaffolds were assessed and were compared with the NIPS-based 3D plotting scaffold.
