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Hydrogen sulfide-induced enhancement of gastric fundus smooth muscle tone is mediated by voltagedependent potassium and calcium channels in mice 被引量:4
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作者 Xiang-Min Meng Xu Huang +4 位作者 Chun-Mei Zhang Dong-Hai Liu Hong-Li Lu young-chul kim Wen-Xie Xu 《World Journal of Gastroenterology》 SCIE CAS 2015年第16期4840-4851,共12页
AIM:To investigate the effect of hydrogen sulfide(H2S)on smooth muscle motility in the gastric fundus.METHODS:The expression of cystathionineβ-synthase(CBS)and cystathionineγ-lyase(CSE)in cultured smooth muscle cell... AIM:To investigate the effect of hydrogen sulfide(H2S)on smooth muscle motility in the gastric fundus.METHODS:The expression of cystathionineβ-synthase(CBS)and cystathionineγ-lyase(CSE)in cultured smooth muscle cells from the gastric fundus was examined by the immunocytochemistry technique.The tension of the gastric fundus smooth muscle was recorded by an isometric force transducer under the condition of isometric contraction with each end of the smooth muscle strip tied with a silk thread.Intracellular recording was used to identify whether hydrogen sulfide affects the resting membrane potential of the gastric fundus in vitro.Cells were freshly separated from the gastric fundus of mice using a variety of enzyme digestion methods and whole-cell patch-clamp technique was used to find the effects of hydrogen sulfide on voltage-dependent potassium channel and calcium channel.Calcium imaging with fura-3AM loading was used to investigate the mechanism by which hydrogen sulfide regulates gastric fundus motility in cultured smooth muscle cells.RESULTS:We found that both CBS and CSE were expressed in the cul tured smooth muscle cel ls from the gastric fundus and that H2S increased the smooth muscle tension of the gastric fundus in mice at low concentrations.In addition,nicardipine and aminooxyacetic acid(AOAA),a CBS inhibitor,reduced the tension,whereas Nω-nitro-L-arginine methyl ester,a nonspecific nitric oxide synthase,increased the tension.The AOAA-induced relaxation was significantly recovered by H2S,and the Na HS-induced increase in tonic contraction was blocked by 5 mmol/L4-aminopyridine and 1μmol/L nicardipine.Na HS significantly depolarized the membrane potential and inhibited the voltage-dependent potassium currents.Moreover,Na HS increased L-type Ca2+currents and caused an elevation in intracellular calcium([Ca2+]i).CONCLUSION:These findings suggest that H2S may be an excitatory modulator in the gastric fundus in mice.The excitatory effect is mediated by voltagedependent potassium and L-type calcium channels. 展开更多
关键词 GASTRIC FUNDUS smooth muscle Hydrogensulfide Tension Voltage-dependent POTASSIUM CHANNEL L-TYPE calcium CHANNEL
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Atrial natriuretic peptide signal pathway upregulated in stomach of streptozotocin-induced diabetic mice 被引量:5
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作者 young-chul kim 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第1期48-55,共8页
AIM:To investigate atrial natriuretic peptide(ANP) secretion from gastric mucosa and the relationship between the ANP/natriuretic peptide receptor type A (NPR-A)pathway and diabetic gastroparesis. METHODS:Male imprint... AIM:To investigate atrial natriuretic peptide(ANP) secretion from gastric mucosa and the relationship between the ANP/natriuretic peptide receptor type A (NPR-A)pathway and diabetic gastroparesis. METHODS:Male imprinting control region(ICR)mice (4 wk old)were divided into two groups:control mice, and streptozotocin-induced diabetic mice.Eight weeks after injection,spontaneous gastric contraction was recorded by using physiography in control and streptozotocin-induced diabetic mice.The ANP-positive cells in gastric mucosa and among dispersed gastric epithelial cells were detected by using immunohistochemistry and flow cytometry,respectively.ANP and natriureticpeptide receptor type A(NPR-A)gene expression in gastric tissue was observed by using the reverse transcriptase polymerase chain reaction. RESULTS:The frequency of spontaneous gastric contraction was reduced from 12.9±0.8 cycles/min in the control group to 8.4±0.6 cycles/min in the diabetic mice(n=8,P<0.05).However,the amplitude of contraction was not significantly affected in the diabetic group.The depletion of interstitial cells of Cajal in the gastric muscle layer was observed in the diabetic mice.ANP-positive cells were distributed in the gastric mucosal layer and the density index of ANP-positive cells was increased from 20.9±2.2 cells/field in control mice to 51.8±2.9 cells/field in diabetic mice(n=8, P<0.05).The percentage of ANP-positive cells among the dispersed gastric epithelial cells was increased from 10.0%±0.9%in the control mice to 41.2%± 1.0%in the diabetic mice(n=3,P<0.05).ANP and NPR-A genes were both expressed in mouse stomach, and the expression was significantly increased in the diabetic mice. CONCLUSION:These results suggest that the ANP/ NPR-A signaling pathway is upregulated in streptozotocin-induced diabetic mice,and contributes to the development of diabetic gastroparesis. 展开更多
关键词 Diabetes mellitus Atrial natriuretic peptide Gastric mucosa GASTROPARESIS
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Gastric nNOS reduction accompanied by natriuretic peptides signaling pathway upregulation in diabetic mice 被引量:2
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作者 Hong-Li Lu Xu Huang +5 位作者 Yi-Song Wu Chun-Mei Zhang Xiang-Min Meng Dong-Hai Liu young-chul kim Wen-Xie Xu 《World Journal of Gastroenterology》 SCIE CAS 2014年第16期4626-4635,共10页
AIM: To investigate the relationship between neuronal nitric oxide synthase (nNOS) expression and the natriuretic peptide signaling pathway in the gastric fundus of streptozotocin (STZ)-induced diabetic mice.
关键词 Diabetic gastroparesis Natriuretic peptides Nitric oxide synthase Enteric neuron
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Asian facial recontouring surgery
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作者 Jong-Woo Choi young-chul kim 《Plastic and Aesthetic Research》 2023年第1期83-95,共13页
Most East Asian women prefer a smaller and smoother facial contour.To meet this aesthetic preference,the concept and surgical techniques of modern facial bone contouring surgery have evolved.Initially,facial bone cont... Most East Asian women prefer a smaller and smoother facial contour.To meet this aesthetic preference,the concept and surgical techniques of modern facial bone contouring surgery have evolved.Initially,facial bone contouring surgery was limited to procedures like mandibular angle and malar reductions.However,contemporary Asian facial bone contouring surgery now places a stronger emphasis on addressing the entire facial profile from a three-dimensional perspective,a concept referred to as Profiloplasty.In this article,we review the evolution of surgical techniques in Asian facial bone contouring surgery and present its current surgical concepts,particularly focusing on the three-dimensional aspects. 展开更多
关键词 ASIAN bone contouring profiloplasty mandibuloplasty malarplasty GENIOPLASTY
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