Effect of Chromium-enhanced Diffusivity on Reverted Transformation in Metastable Austenitic Stainless Steels:Theoretical Calculation and Experiment

Effects of nucleation sites and diffusivity enhancement of chromium on reverted transformation of AISI 304 stainless steel during annealing process were investigated.Dynamics calculation revealed that the reverted transformation of strain-inducedα’-martensite→γaustenite could were closely associated with active nucleation sites and diffusivity enhancement of chromium in nanocrystallineα’-martensite.The experimental data and the results were in accordance with 2-grain austenite/α’-martensite junctions calculated theoretically,which could result from high chromium diffusion rate in nanocrystallineα’-martensite.In addition,low temperature is not conducive to reversed transformation,while high temperature and long annealing time will lead to inhomogeneous grain size distribution.

易学革命与新易学的创建:上博简《周易》形态谱系新探

上博简《周易》是目前所见最早的《周易》文本,其文本形态与《连山》《归藏》皆不相同。从源流谱系来看,上博简《周易》是对《连山》《归藏》旧易学改造基础上创建的新易学,是为第一次易学革命,主要体现在以下几个方面:第一,《周易》卦辞不再列举已往实占记录,而由象辞及占辞构成;第二,《周易》卦辞中的占辞具有断占的方向性和条件性限定;第三,《周易》在改造卦辞的同时,还创造了爻辞;第四,象辞的创作;第五,占辞的象辞化。《周易》文本改造的意义在于:第一,《周易》文学性的生成,使其成为后世诸多文体的源头;第二,《周易》思想性的生成,使其从纯粹的筮占之书转变为思想之书;第三,新易学的创建,开创了易学史的新时代。新易学与旧易学的区别是:第一,新易学对六十四卦从卦名到卦辞作了彻底的改造:卦名体现出一个完整的思想体系,卦辞也不再列举往昔名人实占记录;第二,新易学彻底摒弃了《连山》《归藏》旧易学的神启色彩。在《连山》《归藏》旧易学中,吉凶悔吝的断占是神灵的启示,而在《周易》新易学中,吉凶悔吝的出现是有前提和条件的;第三,新易学创建了系统的阴阳哲学和变易哲学。此后孔子开创的“不占”易学,是为第二次易学革命,易学再次开辟新时代。

Calcium-chelating peptides from rabbit bone collagen:characterization,identification and mechanism elucidation

This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating mechanism.Collagen peptides and calcium were extracted from rabbit bone by instant ejection steam explosion(ICSE)combined with enzymatic hydrolysis,followed by chelation reaction to prepare rabbit bone peptide-calcium chelate(RBCP-Ca).The chelating sites were further analyzed by liquid chromatography-tandem mass(LC-MS/MS)spectrometry while the chelating mechanism and binding modes were investigated.The structural characterization revealed that RBCP successfully chelated with calcium ions.Furthermore,LC-MS/MS analysis indicated that the binding sites included both acidic amino acids(Asp and Glu)and basic amino acids(Lys and Arg),Interestingly,three binding modes,namely Inter-Linking,Loop-Linking and Mono-Linking were for the first time found,while Inter-Linking mode accounted for the highest proportion(75.1%),suggesting that chelation of calcium ions frequently occurred between two peptides.Overall,this study provides a theoretical basis for the elucidation of chelation mechanism of calcium-chelating peptides.

Ti/(Ti,Cr)N/CrN multilayer coated 316L stainless steel by arc ion plating as bipolar plates for proton exchange membrane fuel cells

Arc ion plating (AIP) is applied to form Ti/(Ti,Cr)N/CrN multilayer coating on the surface of 316L stainless steel (SS316L) as bipolar plates for proton exchange membrane fuel cells (PEMFCs). The characterizations of the coating are analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Interfacial contact resistance (ICR) between the coated sample and carbon paper is 4.9 m Omega cm(2) under 150 N/cm(2), which is much lower than that of the SS316L substrate. Potentiodynamic and potentiostatic tests are performed in the simulated PEMFC working conditions to investigate the corrosion behaviors of the coated sample. Superior anticorrosion performance is observed for the coated sample, whose corrosion current density is 0.12 mu A/cm(2). Surface morphology results after corrosion tests indicate that the substrate is well protected by the multilayer coating. Performances of the single cell with the multilayer coated SS316L bipolar plate are improved significantly compared with that of the cell with the uncoated SS316L bipolar plate, presenting a great potential for PEMFC application. (C) 2016 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.

Coupled trace element and SIMS sulfur isotope geochemistry of sedimentary pyrite:Implications on pyrite growth of Caixiashan Pb-Zn deposit

Colloform pyrite with core-rim texture is commonly deposited in carbonate platforms associated with the sulfide ores such as the Caixiashan Pb-Zn deposit.However,the genesis of colloform pyrite in Pb-Zn deposits,its growth controls and their geological implication are insufficiently understood.Integration of in-situ trace element and SIMS sulfur isotopes has revealed geochemical variations among these pyrite layers.These colloform pyrite occur as residual phases of core-rim aggregates,the cores are made up of very fine-grained anhedral pyrite particles,with some rims being made up of fine-grained and poorlycrystallized pyrite,while the other rims were featured with euhedral cubic pyrite.which are cemented by fine-grained calcite and/or dolomite with minor quartz.Sulfur isotope analysis shows that some wellpreserved rims have negative δ^34 S values(-28.12‰to-0.49‰),whereas most of the cores and rims have positive δ^34 S values(>0 to+44.28‰;peak at+14.91‰).Integrating with the methane and sulfate were observed in previous fluid inclusion study,we suggest that the 34 S depleted rims were initially formed by bacteria sulfate reduction(BSR),whereas the positive δ^34 S values were resulted from the sulfate reduction driven by anaerobic methane oxidation(AOM).The well-developed authigenic pyrite and calcite may also support the reaction of AOM.Combined with petrographic observations,trace element composition of the colloform pyrite reveals the incorporation and precipitation behavior of those high abundance elements in the pyrite:Pb and Zn were present as mineral inclusion and likely precipitated before Fe,as supported by the time-resolved Pb-Zn signal spikes in most of the analyzed pyrite grains.Other metals,such as Hg,Co and Ni,may have migrated as chloride complexes and entered the pyrite lattice.Arsenic and Sb,generally influenced by complex-forming reactions rather than substitution ones,could also enter the pyrite lattice,or slightly predate the precipitation of colloform pyrite as mineral inclusions,which are controlled by their hydrolysis constant in the ore fluids.The colloform pyrite may have grown inward from the rims.The successive BSR reaction process would enrich H^32/2S in the overlying water column but reduce the metal content,the nucleation of these pyrite rims was featured by strongly negative sulfur isotopes.The following AOM process should be activated by deformation like the turbidity sediment of the mudstone as the sulfide deposition are associated with fault activities that caused the emission of methane migration upward and simultaneously replenishing the metal in the column.The higher AOM reaction rate and the higher metal supply(not only Fe.but with minor other metals such as Pb and Zn) caused by sediment movement enhanced the metal concentration within the pyrite lattice.

Chronic inflammation deteriorates structure and function of collagen fibril in rat temporomandibular joint disc

Collagen is the building component of temporomandibular joint(TMJ) discs and is often affected by inflammation in temporomandibular disorders. The macromechanical properties of collagen are deteriorated by chronic inflammation. However,the mechanism by which inflammation influences disc function remains unknown. The relationship between the ultrastructure and nanomechanical properties of collagen in inflamed discs should be clarified. Seven-week-old female Sprague–Dawley rats were randomly divided into two groups. Chronic TMJ inflammation was induced by intra-articular injection of complete Freund's adjuvant, and samples were harvested after 5 weeks. Picrosirius staining revealed multiple colours under polarized light, which represented alternative collagen bundles in inflamed discs. Using atomic force microscopy scanning, the magnitude of Young's modulus was reduced significantly accompanied with disordered collagen fibril arrangement with porous architecture of inflamed discs. Transmission electron microscopy scanning revealed a non-uniform distribution of collagen fibres, and oversized collagen fibrils were observed in inflamed discs. Fourier transform infrared microspectroscopy revealed a decrease in 1 338 cm^(-1)/amide II area ratio of collagen in different regions. The peak positions of amide I and amide II bands were altered in inflamed discs,indicating collagen unfolding. Our results suggest that sustained inflammation deteriorates collagen structures, resulting in the deterioration of the ultrastructure and nanomechanical properties of rat TMJ discs.

“父母六子”与“八卦相错”:帛书《周易》形态谱系新探

帛书《周易》经传的形态与谱系与传世王弼注本不是一个体系,但该体系从何而来,其谱系如何,受文献材料限制,多数学者认为帛书《周易》经文六十四卦分为八组的文本结构是汉代人的发明。上述观点,随着清华简等易学新材料公布,已无法站住脚。通过清华简《别卦》,可确认帛书《周易》分为八组的文本结构不是汉代人的发明,而是先秦就已存在了。帛书《周易》六十四卦文本谱系与《说卦传》、清华简《别卦》密切相关,其分为八组的卦序结构来源于《说卦传》父母六子学说,其每组内各卦生成方式与京房八宫卦的生成方式完全不同,其生成方式就是《说卦传》的“八卦相错”。帛书《易传》文本形态谱系与传世本易传总体上大多出自孔门易学体系,但也有《连山》《归藏》古易遗说体系的影响,通过帛书《易传》及相关研究,可以确认否定孔子与易传的关系及学界一直存在的《易传》道家主干说都是难以成立的。

写本学视域下安大简《诗经》的几个问题

安大简《诗经》作为战国楚国写本具有明显的写本学特征。安大简《诗经》写本的国风次序、个别国风命名、个别国风篇目及写本分篇等方面均与孔子编定的《诗经》存在明显差异,体现出写本的流动性及个性化特征。安大简《诗经》的国风次序及个别国风的命名是战国时局在诗学上的投影与反映,体现了诗学与政治的统一性。战国时局的变化,是抄写者产生再加工《诗经》的动因,抄写者通过重新编排国风次序表达了某种政治意图。也许这种表达是完全被动的,如将秦排在第三位,也许是来自强秦的政治压力。而将矦、魏两国国风位置前移,则可能是出自抄写者自我意愿,因为这个抄写者很可能就是魏国人。矦风附记为魏国人所作,其性质为后世“跋语”类文字。安大简《诗经》又把今本唐风称为魏风,这是因为今本毛诗唐风实际上是晋诗的缘故。

Education in the Future: The Universal Life of Technology

Future education is highly integrated with technology and highly open education.The rapid development of information technology urges education to embrace the change brought by technology enthusiastically.The alienation of thinking under technology package must not be ignored and guard against the tendency of"technology determinism".Therefore,this paper interprets the irreplaceable life education from three angles:the reform trend,the basic pattern and the practice construction,and makes full use of technology to restore the meaning of life at the same time.

非均匀载荷下页岩气套管抗挤强度全尺寸试验及新机理

为研究页岩气套管非均匀载荷下抗挤强度及失效机理,采用全尺寸试验方法,开展平面非均匀载荷、弧面非均匀载荷、平面载荷和均匀载荷组合、弧面载荷和均匀载荷组合等4种非均匀载荷情况的试验研究。试验结果表明:非均匀载荷下套管失效机理为套管承载非均匀载荷后,引起的直径变化率发生变化。随着载荷的增大,当直径变化率达到临界状态下,套管发生失效,即达到套管临界承载能力;套管直径变化率与套管抗挤强度近似呈反相关,直径变化率越大,套管的抗挤强度越小。当套管直径变化率小于0.5%时,套管抗挤强度降低不明显,当套管直径变化率≥0.5%时,套管抗挤强度影响显著,套管直径变化率范围为1.0%~2.0%,抗挤性能下降15%~20%。建议页岩气套管可以选择厚壁套管,提高抗径向变形能力,或者基于应变设计理论控制套管径向变形,或者采用弹性介质封固方式以减少外力对套管径向变形的影响,而不是一味追求增加套管的抗挤强度。所得结论对页岩气套管选材或减少页岩气套管失效具有指导作用。

快速水雾化降尘技术在隧道爆破中的应用

爆破作业是地铁隧道施工常用的技术手段,但随着隧道长度、深度的延伸,地铁隧道掘进过程中,常出现爆后粉尘弥漫时间长、降尘效率低等问题,严重威胁施工人员的身体健康,降低施工效率。为更加高效快速地减少钻爆法施工过程中产生的粉尘颗粒,提出了一种快速水雾化除尘技术。依托青岛地铁6号线华山1路西侧斜井工程进行现场试验,利用液态CO_(2)吸热产生的相位变化提升储罐内压,形成高速、高压的水射流可以远距离喷射至掌子面处,最终通过雾化的水滴将粉尘颗粒包裹成团,通过重力自降达到快速降尘的目的。研究结果表明:在爆后15 min范围内,快速水雾化除尘技术可使爆破后产生的PM10、TSP浓度的最大降幅分别达到24.84%、27.68%。快速水雾化降尘技术可以短时间内达到爆后除尘的目的,有效改善施工环境,加快施工进度。

Neuronal nitric oxide synthase/reactive oxygen species pathway is involved in apoptosis and pyroptosis in epilepsy

Dysfunction of neuronal nitric oxide synthase contributes to neurotoxicity,which triggers cell death in various neuropathological diseases,including epilepsy.Studies have shown that inhibition of neuronal nitric oxide synthase activity increases the epilepsy threshold,that is,has an anticonvulsant effect.However,the exact role and potential mechanism of neuronal nitric oxide synthase in seizures are still unclear.In this study,we performed RNA sequencing,functional enrichment analysis,and weighted gene coexpression network analysis of the hippocampus of tremor rats,a rat model of genetic epilepsy.We found damaged hippocampal mitochondria and abnormal succinate dehydrogenase level and Na+-K+-ATPase activity.In addition,we used a pilocarpine-induced N2a cell model to mimic epileptic injury.After application of neuronal nitric oxide synthase inhibitor 7-nitroindazole,changes in malondialdehyde,lactate dehydrogenase and superoxide dismutase,which are associated with oxidative stress,were reversed,and the increase in reactive oxygen species level was reversed by 7-nitroindazole or reactive oxygen species inhibitor N-acetylcysteine.Application of 7-nitroindazole or N-acetylcysteine downregulated the expression of caspase-3 and cytochrome c and reversed the apoptosis of epileptic cells.Furthermore,7-nitroindazole or N-acetylcysteine downregulated the abnormally high expression of NLRP3,gasdermin-D,interleukin-1βand interleukin-18.This indicated that 7-nitroindazole and N-acetylcysteine each reversed epileptic cell death.Taken together,our findings suggest that the neuronal nitric oxide synthase/reactive oxygen species pathway is involved in pyroptosis of epileptic cells,and inhibiting neuronal nitric oxide synthase activity or its induced oxidative stress may play a neuroprotective role in epilepsy.

From static to dynamic:live observation of the support system after ischemic stroke by two photon-excited fluorescence laser-scanning microscopy

Ischemic stroke is one of the most common causes of mortality and disability worldwide.However,treatment efficacy and the progress of research remain unsatisfactory.As the critical support system and essential components in neurovascular units,glial cells and blood vessels(including the bloodbrain barrier)together maintain an optimal microenvironment for neuronal function.They provide nutrients,regulate neuronal excitability,and prevent harmful substances from entering brain tissue.The highly dynamic networks of this support system play an essential role in ischemic stroke through processes including brain homeostasis,supporting neuronal function,and reacting to injuries.However,most studies have focused on postmortem animals,which inevitably lack critical information about the dynamic changes that occur after ischemic stroke.Therefore,a high-precision technique for research in living animals is urgently needed.Two-photon fluorescence laser-scanning microscopy is a powerful imaging technique that can facilitate live imaging at high spatiotemporal resolutions.Twophoton fluorescence laser-scanning microscopy can provide images of the whole-cortex vascular 3D structure,information on multicellular component interactions,and provide images of structure and function in the cranial window.This technique shifts the existing research paradigm from static to dynamic,from flat to stereoscopic,and from single-cell function to multicellular intercommunication,thus providing direct and reliable evidence to identify the pathophysiological mechanisms following ischemic stroke in an intact brain.In this review,we discuss exciting findings from research on the support system after ischemic stroke using two-photon fluorescence laser-scanning microscopy,highlighting the importance of dynamic observations of cellular behavior and interactions in the networks of the brain’s support systems.We show the excellent application prospects and advantages of two-photon fluorescence laser-scanning microscopy and predict future research developments and directions in the study of ischemic stroke.

Backstepping Sliding Mode Control Based on Extended State Observer for Hydraulic Servo System

Hydraulic servo system plays an important role in industrial fields due to the advantages of high response,small size-to-power ratio and large driving force.However,inherent nonlinear behaviors and modeling uncertainties are the main obstacles for hydraulic servo system to achieve high tracking perfor-mance.To deal with these difficulties,this paper presents a backstepping sliding mode controller to improve the dynamic tracking performance and anti-interfer-ence ability.For this purpose,the nonlinear dynamic model is firstly established,where the nonlinear behaviors and modeling uncertainties are lumped as one term.Then,the extended state observer is introduced to estimate the lumped distur-bance.The system stability is proved by using the Lyapunov stability theorem.Finally,comparative simulation and experimental are conducted on a hydraulic servo system platform to verify the efficiency of the proposed control scheme.

宣肺败毒汤治疗新型冠状病毒感染肺炎的随机对照试验

[目的]评价宣肺败毒汤治疗普通型新型冠状病毒感染(COVID-19)的有效性和安全性。[方法]将符合条件的普通型COVID-19患者随机分为A组(宣肺败毒汤联合常规治疗)、B组(甘露消毒汤联合常规治疗)、C组(仅常规治疗)。疗程14天。主要结局指标为SARS-CoV-2核酸转阴时间和住院天数。次要结局指标是症状消失率。安全性指标为药物相关不良事件。[结果]共纳入103例普通型COVID-19患者,随机分为A组(34例)、B组(35例)和C组(34例)。SARS-CoV-2核酸转阴时间最短的是A组(9.88±3.62天),其次是C组(11.20±2.93天)和B组(12.69±4.11天)。三组间差异有统计学意义(P=0.010)。A组住院天数最短(14.00±6.55天),其次是B组(15.40±4.02天)和C组(16.38±5.73天)。组间差异有统计学意义(P=0.019)。各组症状消失率比较,差异无统计学意义(P>0.05)。三组均未发生药物相关不良事件。[结论]宣肺败毒汤治疗普通型COVID-19安全有效,可缩短SARS-CoV-2核酸转阴时间及住院时间。

不稳定型心绞痛患者冠状动脉病变严重程度与窦性心率震荡相关性

目的 探讨不稳定型心绞痛(UAP)患者窦性心率震荡现象(HRT)与冠状动脉(冠脉病变严重程度的相关性及对UAP的诊断价值。方法 本研究为回顾性观察性研究,连续纳入自2019年8月至2021年4月于武汉大学人民医院心血管内科住院UAP患者和冠脉粥样硬化患者,术前完善24 h动态心电图HRT的参数[震荡斜率(TS)、震荡初始(TO)],并于住院期间行冠脉造影,根据冠脉造影结果将其分为UAP组和冠脉硬化组。基于Gensini评分评估UAP患者冠脉病变严重程度与HRT的相关性。使用ROC曲线评估HRT对UAP患者的诊断价值。采用多重Logistic回归的统计方法分析HRT与UAP患者的关系。应用多因素线性回归分析HRT与冠脉病变严重程度之间的关系。结果 共入选198例患者。(1)在单因素分析中,UAP组的TO指标高于冠脉硬化组,而TS指标低于冠脉硬化组(P<0.05),在多因素Logistic回归分析中,TO(OR=1.229,95%CI:1.030~1.468,P=0.022)和TS(OR=0.843,95%CI:0.757~0.939,P=0.002)是不稳定型心绞痛患者的独立影响因素(P<0.05)。(2)多因素线性回归分析显示,TO(B=3.136,P=0.015)、TS(B=-1.439,P=0.045)均是患者的Gensini评分影响因素。(3)ROC曲线分析显示,TO、TS对UAP患者均有一定的预测能力(P<0.05)。结论 HRT是UAP患者的独立影响因素,有助于鉴别UAP患者,且在UAP患者中,HRT是冠脉病变程度的影响因素。

心率减速力、连续心率减速力和不稳定型心绞痛及其病变严重程度的相关性

目的探讨心率减速力(DC)、连续心率减速力(DRs)和不稳定型心绞痛(UAP)及其冠状动脉(冠脉)病变严重程度的相关性。方法连续纳入2020年5月至2021年3月于武汉大学人民医院收治的疑似心绞痛住院患者248例,依据冠脉造影结果分为UAP组181例和冠脉粥样硬化组67例,术前完善24 h动态心电图以获取DC及DRs,比较两组DC、DRs。以Gensini评分评估UAP患者冠脉病变严重程度,有序多分类Logistic回归分析DC、DRs与冠脉病变严重程度的相关性。结果单因素分析中,UAP组的DC、DR2、DR4及DR8低于冠脉硬化组(P<0.05),多因素Logistic回归分析中,DC(OR=0.828,95%CI:0.688~0.998)、DR4(OR=0.209,95%CI:0.046~0.954)及DR8(OR=0.971,95%CI:0.958~0.984)是UAP的独立影响因素(P<0.05)。根据Gensini评分以三分位法分为三组。单因素分析显示Gensini评分越高,患者DC、DR2、DR4和DR8水平越低,有序多分类Logistic回归分析显示DC(OR=0.795,95%CI:0.662~0.952)、DR2(OR=0.677,95%CI:0.568~0.807)、DR4(OR=0.033,95%CI:0.005~0.205)和DR8(OR=0.969,95%CI:0.947~0.991)是患者Gensini评分的独立影响因素(P均<0.05)。结论DC、DRs是UAP患者危险分层的独立影响因素,与其冠脉病变严重程度密切相关。

Landslide susceptibility assessment in Western Henan Province based on a comparison of conventional and ensemble machine learning

Landslide is a serious natural disaster next only to earthquake and flood,which will cause a great threat to people’s lives and property safety.The traditional research of landslide disaster based on experience-driven or statistical model and its assessment results are subjective,difficult to quantify,and no pertinence.As a new research method for landslide susceptibility assessment,machine learning can greatly improve the landslide susceptibility model’s accuracy by constructing statistical models.Taking Western Henan for example,the study selected 16 landslide influencing factors such as topography,geological environment,hydrological conditions,and human activities,and 11 landslide factors with the most significant influence on the landslide were selected by the recursive feature elimination(RFE)method.Five machine learning methods[Support Vector Machines(SVM),Logistic Regression(LR),Random Forest(RF),Extreme Gradient Boosting(XGBoost),and Linear Discriminant Analysis(LDA)]were used to construct the spatial distribution model of landslide susceptibility.The models were evaluated by the receiver operating characteristic curve and statistical index.After analysis and comparison,the XGBoost model(AUC 0.8759)performed the best and was suitable for dealing with regression problems.The model had a high adaptability to landslide data.According to the landslide susceptibility map of the five models,the overall distribution can be observed.The extremely high and high susceptibility areas are distributed in the Funiu Mountain range in the southwest,the Xiaoshan Mountain range in the west,and the Yellow River Basin in the north.These areas have large terrain fluctuations,complicated geological structural environments and frequent human engineering activities.The extremely high and highly prone areas were 12043.3 km^(2)and 3087.45 km^(2),accounting for 47.61%and 12.20%of the total area of the study area,respectively.Our study reflects the distribution of landslide susceptibility in western Henan Province,which provides a scientific basis for regional disaster warning,prediction,and resource protection.The study has important practical significance for subsequent landslide disaster management.

A bioartificial transgenic porcine whole liver expressing human proteins alleviates acute liver failure in pigs

Background:Preventing heterologous protein influx in patients is important when using xenogeneic bioartificial livers(BALs)to treat liver failure.The development of transgenic porcine livers synthesizing human proteins is a promising approach in this regard.Here,we evaluated the safety and efficacy of a transgenic porcine liver synthesizing human albumin(h ALB)and coagulation factor VII(h FVII)within a bioartificial system.Methods:Tibetan miniature pigs were randomly subjected to different interventions after surgeryinduced partially ischemic liver failure.Group A(n=4)was subjected to basic treatment;group B(n=4)was to standard medical treatment and wild-type porcine BAL perfusion,and group C(n=2)was to standard medical treatment and transgenic BAL perfusion.Biochemical parameters,coagulation status,survival time,and pathological changes were determined.Expressions of h ALB and h FVII were detected using immunohistochemistry and enzyme-linked immunosorbent assays.Results:The survival time in group A was 9.75±1.26 days;this was shorter than that in both perfused groups,in which all animals reached an endpoint of 12 days(P=0.006).Ammonia,bilirubin,and lactate levels were significantly decreased,whereas albumin and fibrinogen levels were increased after perfusion(all P<0.05).h ALB and h FVII were detected in transgenic BAL-perfused pig serum and ex vivo in the liver tissues.Conclusions:The humanized transgenic pig livers could synthesize and secrete h ALB and h FVII ex vivo in a whole organ-based bioartificial system,while maintaining their metabolism,detoxification,transformation,and excretion functions,which were comparable to those observed in wild-type porcine livers.Therefore,the use of transgenic bioartificial whole livers is expected to become a new approach in treating acute liver failure.

Willis covered stent in the treatment of a recurrent blood blister-like aneurysm after pipeline implementation:A case report

Blood blister-like aneurysms(BBAs)are fragile and difficult to treat.However,the optimal treatment has yet to be determined.Pipeline embolization devices and Willis covered stent implementation are still controversial strategies for treating BBA.Herein,we report a case of recurrent BBA successfully treated with a Willis covered stent.A long-term follow-up angiography after the procedure indicated complete occlusion of the aneurysm.This case demonstrates the safety and efficacy of applying the Wills cover stent in the treatment of recurrent BBA after Pipeline implantation.