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Histological,transcriptomic,and gene functional analyses reveal the regulatory events underlying gibberellin-induced parthenocarpy in tomato
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作者 Shaobo Niu yu he +3 位作者 Siwei Yan Zhengliang Sun Run Cai Yan Zhang 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期156-170,共15页
Gibberellin(GA)is one of the major plant hormones that promote parthenocarpy,a highly valuable agronomic trait.Here,we demonstrated that exogenous GA3application triggered the formation of parthenocarpic fruits with s... Gibberellin(GA)is one of the major plant hormones that promote parthenocarpy,a highly valuable agronomic trait.Here,we demonstrated that exogenous GA3application triggered the formation of parthenocarpic fruits with smaller size but unchanged shape in tomato(Solanum lycopersicum).These fruits exhibited a thicker pericarp,undeveloped ovules,and few jelly tissues,leading to smaller locules with empty cavities.Histological investigation showed that GA treatment produced more cell layers with larger cells in the pericarp,suggesting its promotion in both cell division and expansion.Transcriptomic analyses between GA-3and mock-treated unpollinated ovaries/fruits identified a large number of differentially expressed genes related to hormones,cell division,cell expansion,and transcription factors,implying that they coordinately regulated parthenocarpy conferred by GA.In particular,the downregulation of five reported repressors of tomato parthenocarpy,including two auxin signaling components,AUXIN RESPONSE FACTOR5(SlARF5)and Sl ARF7,and three MADS-box genes,TOMATO APETALA3(TAP3),TOMATO PISTILLATA(TPI),and AGAMOUS-LIKE6(SlAGL6),after GA treatment might play a key role in this process.Furthermore,we found that the knockdown of a GA signaling factor SlMYB33,which was depressed by GA treatment,induced parthenocarpic fruit set in tomato,an effect that might have been achieved by enhancing GA biosynthesis and decreasing the expression of some repressors of tomato parthenocarpy.Thus,our results provide a basis for understanding the regulatory mechanism of GA in tomato parthenocarpy. 展开更多
关键词 TOMATO GIBBERELLIN PARTHENOCARPY HISTOLOGY Transcriptome SlMYB33
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Metabolomics: A useful tool for ischemic stroke research 被引量:1
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作者 Wentao Li Chongyu Shao +5 位作者 Chang Li Huifen Zhou Li yu Jiehong Yang Haitong Wan yu he 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2023年第9期968-983,共16页
Ischemic stroke(IS)is a multifactorial and heterogeneous disease.Despite years of studies,effective strategies for the diagnosis,management and treatment of stroke are still lacking in clinical practice.Metabolomics i... Ischemic stroke(IS)is a multifactorial and heterogeneous disease.Despite years of studies,effective strategies for the diagnosis,management and treatment of stroke are still lacking in clinical practice.Metabolomics is a growing field in systems biology.It is starting to show promise in the identification of biomarkers and in the use of pharmacometabolomics to help patients with certain disorders choose their course of treatment.The development of metabolomics has enabled further and more biological applications.Particularly,metabolomics is increasingly being used to diagnose diseases,discover new drug targets,elucidate mechanisms,and monitor therapeutic outcomes and its potential effect on precision medicine.In this review,we reviewed some recent advances in the study of metabolomics as well as how metabolomics might be used to identify novel biomarkers and understand the mechanisms of IS.Then,the use of metabolomics approaches to investigate the molecular processes and active ingredients of Chinese herbal formulations with anti-IS capabilities is summarized.We finally summarized recent developments in single cell metabolomics for exploring the metabolic profiles of single cells.Although the field is relatively young,the development of single cell metabolomics promises to provide a powerful tool for unraveling the pathogenesis of IS. 展开更多
关键词 Metabolomics Ischemic stroke BIOMARKER Precision medicine Single cell metabolomics
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基于非监督对比学习的火星地形特征提取方法
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作者 杨博 魏翔 +1 位作者 于贺 刘超凡 《北京航空航天大学学报》 EI CAS CSCD 北大核心 2024年第6期1842-1849,共8页
火星表面地形智能识别对火星车自主探测具有重要意义,火星地形图像的特征提取方法目前主要分为传统的浅层视觉特征提取和基于监督学习的深层特征提取2类。找回丢失图像信息、获取大量带标签数据是要解决的关键问题。为此,提出一种基于... 火星表面地形智能识别对火星车自主探测具有重要意义,火星地形图像的特征提取方法目前主要分为传统的浅层视觉特征提取和基于监督学习的深层特征提取2类。找回丢失图像信息、获取大量带标签数据是要解决的关键问题。为此,提出一种基于非监督对比学习的火星地形特征识别方法,通过建立图像字典数据集,用“问询”和“编码”2组神经网络分别将单个图像与“字典”数据集中其他图像进行对比,用相似度泛函作为损失函数对网络进行训练,从而实现对火星地形图像的特征识别。所提方法还具有对训练数据集之外的新类型地形图像识别能力,后续识别分类优越性突出。仿真结果表明:所提方法识别准确率为85.4%,对新类型地形图像的识别准确率为84.5%。 展开更多
关键词 对比学习 非监督 深度学习 火星地形 特征提取
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A Data-Driven Oil Production Prediction Method Based on the Gradient Boosting Decision Tree Regression
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作者 Hongfei Ma Wenqi Zhao +1 位作者 yurong Zhao yu he 《Computer Modeling in Engineering & Sciences》 SCIE EI 2023年第3期1773-1790,共18页
Accurate prediction ofmonthly oil and gas production is essential for oil enterprises tomake reasonable production plans,avoid blind investment and realize sustainable development.Traditional oil well production trend... Accurate prediction ofmonthly oil and gas production is essential for oil enterprises tomake reasonable production plans,avoid blind investment and realize sustainable development.Traditional oil well production trend prediction methods are based on years of oil field production experience and expertise,and the application conditions are very demanding.With the rapid development of artificial intelligence technology,big data analysis methods are gradually applied in various sub-fields of the oil and gas reservoir development.Based on the data-driven artificial intelligence algorithmGradient BoostingDecision Tree(GBDT),this paper predicts the initial single-layer production by considering geological data,fluid PVT data and well data.The results show that the GBDT algorithm prediction model has great accuracy,significantly improving efficiency and strong universal applicability.The GBDTmethod trained in this paper can predict production,which is helpful for well site optimization,perforation layer optimization and engineering parameter optimization and has guiding significance for oilfield development. 展开更多
关键词 Gradient boosting decision tree production prediction data analysis
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基于六维辨证观拆解慢性阻塞性肺疾病辨治体系
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作者 王雨菡 于河 +3 位作者 张文静 王振东 李勋欣 谷晓红 《世界中医药》 CAS 北大核心 2024年第9期1286-1292,共7页
六维辨证观是综合多种辨证思维所提出的辨证观念,旨在从病因、病位、病期、病性、病势、病理等6个维度对疾病证态进行系统辨析,有利于临床把握整体,抓住机要。慢性阻塞性肺疾病(COPD)是一种以进行性气流受限和持续的呼吸道症状为特征的... 六维辨证观是综合多种辨证思维所提出的辨证观念,旨在从病因、病位、病期、病性、病势、病理等6个维度对疾病证态进行系统辨析,有利于临床把握整体,抓住机要。慢性阻塞性肺疾病(COPD)是一种以进行性气流受限和持续的呼吸道症状为特征的呼吸系统疾病,发病多责之于肺气本虚,内外感召或他脏及肺,常有慢性肺系疾病迁延日久或先天不足因素。病位主要在肺,最常波及脾肾,病久可牵连心肝及六腑,是典型的多系统复杂疾病。基本病机是本虚标实,即肺脾肾虚损,痰瘀互结成积,除见气虚气滞、阴阳虚损等病理状态外,还最常见痰饮瘀及微型癥瘕等病理产物。本病分期明确,卫气同病、气分期和气营同病阶段病情寒热之性及邪正之势多变,可因病邪性质、季节地域、体质等因素表现不一,需要谨慎判断和用药;气血同病阶段寒热错杂、虚实并见,治疗棘手,恰是中医药发挥优势的大好时机。 展开更多
关键词 六维辨证观 慢阻肺 急性加重期 稳定期 本虚标实 痰瘀互结 脏腑虚损 扶正祛邪
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广西北山铅锌矿床矿体形貌及其成矿指示
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作者 董海雨 余何 +2 位作者 苟晓利 高友君 张亦弛 《桂林理工大学学报》 CAS 北大核心 2024年第1期19-28,共10页
广西北山铅锌矿床是桂北地区储量最大的铅锌矿床,开采历史悠久,为矿体形貌研究提供了良好条件。基于野外地质调查和前人研究成果,认为矿床的形成先后经历了层间滑动、倾伏褶皱和断裂3个构造变形阶段。矿体形貌研究发现,矿床从南到北具... 广西北山铅锌矿床是桂北地区储量最大的铅锌矿床,开采历史悠久,为矿体形貌研究提供了良好条件。基于野外地质调查和前人研究成果,认为矿床的形成先后经历了层间滑动、倾伏褶皱和断裂3个构造变形阶段。矿体形貌研究发现,矿床从南到北具有不同的侧伏向和侧伏角,南部矿体向SW侧伏、侧伏角20°;中部矿体延伸近SN向,侧伏不明显;北部矿体向NE侧伏,侧伏角为10°。矿体形貌成因类型包括岩溶型矿体(Ⅰ号矿体群)、构造-岩溶型矿体(Ⅱ号矿体群)、构造型矿体(Ⅲ号矿体群)和流体型矿体(少量小矿体)。通过矿体形貌类型、成矿构造、矿体力性、力向、力度、韵度、时空等构造成矿参数研究,综合评价认为矿区具有较好的成矿条件,矿体形貌等级达到“良好”,推测矿床形成于印支期或之前。根据矿体形貌评价结果建立找矿模式,建议矿区下一步找矿方向应在已知Ⅲ号矿体群南部深处侧伏方向以及沿NNE向F3断层上盘或下盘寻找构造型矿体。 展开更多
关键词 矿体形貌 成矿构造 古岩溶 层滑 找矿模式 北山铅锌矿床 广西
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广西泗顶铅锌矿床矿体形貌特征及构造成因分析
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作者 崔元钰 余何 +3 位作者 苟晓利 汪帆 徐敏杰 蔡瑞琪 《西北地质》 CAS CSCD 北大核心 2024年第2期121-134,共14页
研究矿体形貌分类,鉴别控制矿体的成矿构造类型,是成矿构造研究与预测的基础性工作,在矿山深部找矿工作中具有指导意义。广西泗顶铅锌矿床是桂北铅锌成矿区中大型矿床的典型代表,经过多年开采,其矿体的三维形貌已展露无遗,具有进行矿体... 研究矿体形貌分类,鉴别控制矿体的成矿构造类型,是成矿构造研究与预测的基础性工作,在矿山深部找矿工作中具有指导意义。广西泗顶铅锌矿床是桂北铅锌成矿区中大型矿床的典型代表,经过多年开采,其矿体的三维形貌已展露无遗,具有进行矿体形貌及构造成因研究的良好条件。基于矿体形貌视角,通过矿体形貌的几何特征及成因特征解析,建立矿体的构造成矿模式,分析讨论矿床的构造成因与动力学背景。研究结果表明:①泗顶矿区成矿构造类型分为两类,一类为早期构造流体型成矿构造,控制脉状铅锌矿体;另一类为晚期构造型成矿构造,控制蚀变岩型贫矿体,并叠加于早期脉状矿体之上。②矿体形貌由NE向右行张扭性断层控制形成的构造顺层脉型、NW向右行张性断层控制形成的构造切层脉型和脆–韧性剪切带控制形成的构造–流体型3种矿体形貌组成。基于矿体形貌建立以Ⅰ、Ⅳ号矿体为代表的构造顺层式成矿模式和以Ⅴ号矿体代表的构造切层式成矿模式。③区域动力学表明,泗顶矿区经历了多期构造运动,尤其是印支期的两次动力转换(早期SE向区域挤压力转换为EW向右行走滑剪切,晚期近EW向右行剪切则转换为EW向挤压),为矿床的形成提供了优越的动力条件。④矿田动力学分析认为,泗顶铅锌成矿作用发生在脆–韧性剪切带演化到脆性阶段,应力弱化、变形分解、递进变形、非共轴剪切和层间滑动共同控制了各类几何矿体形貌的形成。 展开更多
关键词 矿体形貌 成矿构造 区域动力学 铅锌矿 广西泗顶
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核壳结构上转换发光纳米粒子的合成及用于细胞色素C的检测
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作者 董佳瑶 易静 +2 位作者 刘浏 于贺 唐宏武 《分析科学学报》 CAS CSCD 北大核心 2024年第2期125-131,共7页
本工作合成了三层核壳结构的上转换发光纳米粒子,其中第一层是惰性核,第二层为发光层,为了增强上转换发光强度,又在表面包覆了第三层惰性壳层。该材料粒径均一、分散性良好,其发光层厚度约为2.4nm,惰性壳层厚度约为2.9nm。在其表面修饰... 本工作合成了三层核壳结构的上转换发光纳米粒子,其中第一层是惰性核,第二层为发光层,为了增强上转换发光强度,又在表面包覆了第三层惰性壳层。该材料粒径均一、分散性良好,其发光层厚度约为2.4nm,惰性壳层厚度约为2.9nm。在其表面修饰了细胞色素C的适配体链及互补链,在适配体的3’端修饰了BHQ3基团,能够猝灭上转换纳米粒子在655nm波长处的发光。当细胞色素C存在时,适配体与细胞色素C结合从而离开其表面,使655nm处的发光恢复。在检测过程中,540nm处的发光强度不会发生变化,可以用作细胞色素C的比率荧光检测。结果表明,当细胞色素C浓度在5~80μmol/L范围时,发光信号恢复程度与细胞色素C浓度呈线性相关,相关系数为0.998,检出限为1μmol/L。所建立方法可为细胞色素C的荧光检测提供一种新的技术思路。 展开更多
关键词 上转换发光纳米粒子 细胞色素C 发光共振能量转移
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向内凝聚:铸牢中华民族共同体意识的文化主体性意蕴 被引量:1
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作者 俞贺 魏莉 《西北民族大学学报(哲学社会科学版)》 北大核心 2024年第1期30-42,共13页
中华民族的文化主体性表现为中华民族对中华文化独立自主的主人翁意识与自信、自觉、自为的历史主动精神。巩固文化主体性,应在“第二个结合”中着力赓续中华文脉,坚定文化自信,秉持开放包容,坚持守正创新,破解古今中西之争,不断建设中... 中华民族的文化主体性表现为中华民族对中华文化独立自主的主人翁意识与自信、自觉、自为的历史主动精神。巩固文化主体性,应在“第二个结合”中着力赓续中华文脉,坚定文化自信,秉持开放包容,坚持守正创新,破解古今中西之争,不断建设中华民族现代文明。巩固文化主体性与铸牢中华民族共同体意识内在耦合,旨在巩固中华文明的统一性与中华文化的一体性,并为“五个认同”提供坚实保障、为“四个与共”开拓精神磁场、为中华民族共有精神家园建造思想城墙、为各民族交往交流交融打通时空场域。发挥中华文化“向内凝聚”的作用,为铸牢中华民族共同体意识增量赋能,促进各民族文化之间相互影响、相互转化、相互融通、有机统一,不断建设中华文化共同体。 展开更多
关键词 文化主体性 铸牢中华民族共同体意识 向内凝聚 中华民族共有精神家园
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基于层级化注意力融合的航拍图像电力线分割模型
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作者 徐丹 余南南 +2 位作者 张嘉睿 于贺 于金扣 《南方电网技术》 CSCD 北大核心 2024年第4期162-170,共9页
电力线自动分割是保证智能检测平台安全运行的重要前提。然而,电力线分割是复杂背景、多种气候环境下的小目标分割问题,极易出现误检、漏检问题。为了提高电力线分割的鲁棒性与准确性,结合编码器-解码器框架,提出了一种基于层级化注意... 电力线自动分割是保证智能检测平台安全运行的重要前提。然而,电力线分割是复杂背景、多种气候环境下的小目标分割问题,极易出现误检、漏检问题。为了提高电力线分割的鲁棒性与准确性,结合编码器-解码器框架,提出了一种基于层级化注意力融合的端到端分割模型。该模型提出一种降维残差卷积单元,增加网络深度的同时大幅度减少网络参数,更易部署于嵌入式设备。为了使模型捕捉到全局信息并强调电力线的目标区域,设计了链式层级化注意力融合模块进行多尺度特征融合,以解决类别不均衡问题。为了提高模型对电力线特有的直线先验特征的关注,提出了直线先验损失函数,并与Focal、Dice损失函数组合成联合损失函数,进一步提高了电力线分割准确度。实验结果表明,所提模型网络深度增加为基础网络的2.8倍左右,而参数量仅为原来的1/3左右。针对常规天气和雾天环境下的航拍图像均能实现电力线的鲁棒分割。所提出模型能应用于电力巡检领域,使巡检更加智能与高效。 展开更多
关键词 航拍图像 电力线分割 残差卷积 注意力融合 直线先验
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An improved TDE technique for derivation of 2D turbulence structures based on GPI data in toroidal plasma
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作者 王威策 程钧 +12 位作者 石中兵 严龙文 黄治辉 弋开阳 吴娜 何钰 邹千 陈熙 张文 陈建 聂林 季小全 钟武律 《Plasma Science and Technology》 SCIE EI CAS CSCD 2024年第3期131-137,共7页
This paper reports an improved time-delay estimation(TDE)technique for the derivation of turbulence structures based on gas-puff imaging data.The improved TDE technique,integrating an inverse timing search and hierarc... This paper reports an improved time-delay estimation(TDE)technique for the derivation of turbulence structures based on gas-puff imaging data.The improved TDE technique,integrating an inverse timing search and hierarchical strategy,offers superior accuracy in calculating turbulent velocity field maps and analyzing blob dynamics,which has the power to obtain the radial profiles of equilibrium poloidal velocity,blob size and its radial velocity,even the fluctuation analysis,such as geodesic acoustic modes and quasi-coherent mode,etc.This improved technique could provide important 2D information for the study of edge turbulence and blob dynamics,advancing the understanding of edge turbulence physics in fusion plasmas. 展开更多
关键词 gas-puff imaging TDE method TURBULENCE velocity field map
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Bowel preparation protocol for hospitalized patients ages 50 years or older:A randomized controlled trial
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作者 yu he Qi Liu +3 位作者 Yi-Wen Chen Li-Jian Cui Kai Cao Zi-Hao Guo 《World Journal of Gastrointestinal Endoscopy》 2024年第1期18-28,共11页
BACKGROUND The incidence and mortality rate of colorectal cancer progressively increase with age and become particularly prominent after the age of 50 years.Therefore,the population that is≥50 years in age requires l... BACKGROUND The incidence and mortality rate of colorectal cancer progressively increase with age and become particularly prominent after the age of 50 years.Therefore,the population that is≥50 years in age requires long-term and regular colonoscopies.Uncomfortable bowel preparation is the main reason preventing patients from undergoing regular colonoscopies.The standard bowel preparation regimen of 4-L polyethylene glycol(PEG)is effective but poorly tolerated.AIM To investigate an effective and comfortable bowel preparation regimen for hospitalized patients≥50 years in age.METHODS Patients were randomly assigned to group 1(2-L PEG+30-mL lactulose+a lowresidue diet)or group 2(4-L PEG).Adequate bowel preparation was defined as a Boston bowel preparation scale(BBPS)score of≥6,with a score of≥2 for each segment.Non-inferiority was prespecified with a margin of 10%.Additionally,the degree of comfort was assessed based on the comfort questionnaire.RESULTS The proportion of patients with a BBPS score of≥6 in group 1 was not significantly different from that in group 2,as demonstrated by intention-to-treat(91.2%vs 91.0%,P=0.953)and per-protocol(91.8%vs 91.0%,P=0.802)analyses.Furthermore,in patients≥75 years in age,the proportion of BBPS scores of≥6 in group 1 was not significantly different from that in group 2(90.9%vs 97.0%,P=0.716).Group 1 had higher comfort scores(8.85±1.162 vs 7.59±1.735,P<0.001),longer sleep duration(6.86±1.204 h vs 5.80±1.730 h,P<0.001),and fewer awakenings(1.42±1.183 vs 2.04±1.835,P=0.026)than group 2.CONCLUSION For hospitalized patients≥50 years in age,the bowel preparation regimen comprising 2-L PEG+30-mL lactulose+a low-residue diet produced a cleanse that was as effective as the 4-L PEG regimen and even provided better comfort. 展开更多
关键词 Aged 50 years or older HOSPITALIZED 2-L polyethylene-glycol+30-mL lactulose+a low-residue diet COMFORT
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浅圆仓毛毡压盖储藏稻谷实仓试验研究
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作者 田雨申 刘长生 +2 位作者 邹善韬 于鹤 李慧 《现代食品》 2024年第4期11-14,共4页
在辽宁地区,毛毡压盖是储藏粮食应用率较高,且成效显著的科技储粮措施。基于此,本试验对比分析了同仓型2种储粮措施,跟踪记录了试验仓房和对照仓房1个储存周期内的储存品质及经济效益变化。结果表明,毛毡压盖储藏稻谷的效果更好。
关键词 毛毡压盖 浅圆仓 空调控温 粳稻谷
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带血供腓骨瓣与空心螺钉胫距跟融合治疗终末期踝关节炎 被引量:2
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作者 于鹤 郑加法 +1 位作者 宋秀锋 关盛溢 《中国组织工程研究》 CAS 北大核心 2023年第4期588-593,共6页
背景:既往研究报道腓骨瓣支撑结合空心螺钉行胫距跟融合治疗踝关节骨性关节炎有确切效果,但所用腓骨瓣未带血供,在一定程度上影响局部血运恢复。此次研究利用腓骨远端良好血运,对腓骨瓣支撑结合空心螺钉胫距跟融合进行改良,探讨改良术... 背景:既往研究报道腓骨瓣支撑结合空心螺钉行胫距跟融合治疗踝关节骨性关节炎有确切效果,但所用腓骨瓣未带血供,在一定程度上影响局部血运恢复。此次研究利用腓骨远端良好血运,对腓骨瓣支撑结合空心螺钉胫距跟融合进行改良,探讨改良术式治疗踝关节骨性关节炎的临床效果。目的:探讨经腓骨门入路带血供腓骨瓣结合空心螺钉行胫距跟融合治疗终末期踝关节骨性关节炎的临床疗效,以及对患肢生物力学的影响。方法:回顾分析2018年1月至2020年10月大连市第二人民医院收治的30例终末期踝关节骨性关节炎患者,均择期实施经腓骨门入路带血供腓骨瓣结合空心螺钉行胫距跟融合治疗。术后接受12个月随访,观察术后腓骨远端局部血运状况及切口Ⅰ期愈合情况;术后复查踝关节CT,评估骨愈合时间;应用步态分析仪及足底压力测量系统评估术后恢复情况,掌握术后并发症。结果与结论:①患者术后均接受12个月随访,无失访病例;术后多普勒血管探测仪及下肢血管CT血管造影复查显示,患肢血管蒂和腓后动脉穿支及伴行静脉吻合,局部血运良好,切口均为Ⅰ期愈合;②术后复查踝关节CT,融合处骨性愈合时间为8-13周,平均(10.70±2.13)周;③术后3个月患者的步长、步速及步频均较术前显著提升,差异有显著性意义(P<0.05);④通过足底压力测量系统测定分析,术后6个月患者自然行走中,患侧足底第2-4跖骨区、中足、足跟内侧、足跟外侧、全足底的压力值均低于术前,差异有显著性意义(P<0.05);⑤术后随访,1例患者出现腓肠神经损伤症状,在对症治疗后消失;⑥患者踝关节功能恢复优良率为100%;⑦提示经腓骨门入路带血供腓骨瓣结合空心螺钉行胫距跟融合治疗终末期踝关节骨性关节炎,可有效改善局部血运,提高融合效果,促进患肢踝关节功能恢复,有重要的临床意义。 展开更多
关键词 带血供腓骨瓣 空心螺钉 胫距跟融合 踝关节 骨性关节炎 局部血运 骨愈合 足底压力
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Involvement of microRNA-181a and Bim in a rat model of retinal ischemia-reperfusion injury 被引量:4
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作者 yu he Jin-Nan Liu +1 位作者 Jun-Jun Zhang Wei Fan 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2016年第1期33-40,共8页
AIM:To investigate the changes in the expression of micro RNA-181a(mi R-181a)and Bim in a rat model of retinal ischemia-reperfusion(RIR),to explore their target relationship in RIR and their involvement in regula... AIM:To investigate the changes in the expression of micro RNA-181a(mi R-181a)and Bim in a rat model of retinal ischemia-reperfusion(RIR),to explore their target relationship in RIR and their involvement in regulating apoptosis of retinal ganglion cells(RGCs).·M ETHODS:Target gene prediction for mi R-181a was performed with the aid of bioinformatics and Bim was identified as a potential target gene of mi R-181a.A rat model of RIR was created by increasing the intraocular pressure.RGCs in the flatmounted retinas were labeled with Brn3,a marker for alive RGCs,by immunofluorescent staining.The changes in the number of RGCs after RIR were recorded.Quantitative reverse transcription-polymerase chain reaction(q RT-PCR)was used to determine the expression level of mi R-181a in the retina.Bim/Brn3 double immunofluorescence was used to detect the localization of Bim.The expression of Bim in the retina was determined with the aids of Western blot and q RT-PCR.·R ESULTS:Compared with the negative control group,the density of RGCs was significantly lower in the ischemia/reperfusion(I/R)-24h and I/R-72h groups(〈0.001).The expression level of mi R-181a started to decrease at 0h after RIR,and further decreased at 24h and 72h compared with the negative control group(〈0.001).Bim was significantly upregulated at 12h after RIR(〈0.05)and reached peak at 24,72h compared with the negative control group(〈0.01).Pearson correlation analysis showed that the expression level of Bim was negatively correlated with the expression level of mi R-181a and the density of RGCs.·CONCLUSION:Bim may be a potential target gene of mi R-181a.Both mi R-181a and Bim are involved in RGCs death in RIR.RIR may promote RGCs apoptosis in the retina downregulation of mi R-181a and its inhibition on Bim expression. 展开更多
关键词 microRNA-181a BIM retinal ischemiareperfusion target gene retinal ganglion cells APOPTOSIS
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Comparison of the postoperative pain change and spinal stenosis rate between percutaneous vertebroplasty combined with radiofrequency ablation and with ^(125)I particle implantation in the treatment of metastatic spinal cord compression:A retrospective st 被引量:5
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作者 yu he Shilong Han +2 位作者 Chungen Wu Fei Ge Jianbo Wang 《Journal of Interventional Medicine》 2021年第4期197-202,共6页
Background context:Metastatic spinal cord compression(MSCC)seriously affects the survival rate.Objective:The therapeutic effects of two treatment strategies for MSCC:percutaneous vertebroplasty(PVP)combined with radio... Background context:Metastatic spinal cord compression(MSCC)seriously affects the survival rate.Objective:The therapeutic effects of two treatment strategies for MSCC:percutaneous vertebroplasty(PVP)combined with radiofrequency ablation(RFA)and PVP combined with^(125)I particle implantation,were compared.Study design:Retrospective study.Patient sample:40 patients with MSCC were divided into two groups:19 cases in the RFA group and 21 cases in the^(125)I group.Method:All patients were accessed to determine the differences in pain,which was evaluated using the visual analog scale(VAS)at 1 week,1 month,and 3 months after the operation,and spinal stenosis rates(SSRs),which were measured at 1 and 3 months after the operation,between the two groups.Results:The VAS scores and SSRs at baseline were comparable between the RFA group and the^(125)I group(7.19±2.07 vs 7.42±1.95,37.7%±11.2%vs 41.1%±11.4%).The VAS scores and SSRs at 1 month and 3 months after the operation were significantly reduced in both groups,compared with those at baseline.The VAS scores and SSRs in the^(125)I group were lower than those in the RFA group at 3 months after the operation(1.09±0.97 vs 1.75±1.06 p=0.048 and 12.3%±6.4%vs 18.1%±10.1%p=0.034),while the VAS scores at1 week after the operation in the RFA group were lower than those in the^(125)I group(4.39±1.34 vs 5.05±1.82 p=0.049).Conclusion:PVP combined with RFA has a slight advantage in relieving pain in the short term,while PVP combined with^(125)I particle implantation may have a better effect in the relieving pain and decreasing the SSRs at 3 months after the operation. 展开更多
关键词 Metastatic spinal cord compression Pain management VERTEBROPLASTY Radiofrequency ablation ^(125)I particle
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Comparison of acute pneumonia caused by SARS-CoV-2 and other respiratory viruses in children:a retrospective multi-center cohort study during COVID-19 outbreak 被引量:2
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作者 Guang-Li Ren Xian-Feng Wang +27 位作者 Jun Xu Jun Li Qiong Meng Guo-Qiang Xie Bo Huang Wei-Chun Zhu Jing Lin Cheng-he Tang Sheng Ye Zhuo Li Jie Zhu Zhen Tang Ming-Xin Ma Cong Xie Ying-Wen Wu Chen-Xi Liu Fang Yang yu-Zong Zhou Ying Zheng Shu-Ling Lan Jian-Feng Chen Feng Ye yu he BenQing Wu Long Chen Si-Mao Fu Cheng-Zhong Zheng yuan Shi 《Military Medical Research》 SCIE CSCD 2021年第4期468-480,共13页
Background:Until January 18,2021,coronavirus disease-2019(COVID-19)has infected more than 93 million individuals and has caused a certain degree of panic.Viral pneumonia caused by common viruses such as respiratory sy... Background:Until January 18,2021,coronavirus disease-2019(COVID-19)has infected more than 93 million individuals and has caused a certain degree of panic.Viral pneumonia caused by common viruses such as respiratory syncytial virus,rhinovirus,human metapneumovirus,human bocavirus,and parainfluenza viruses have been more common in children.However,the incidence of COVID-19 in children was significantly lower than that in adults.The purpose of this study was to describe the clinical manifestations,treatment and outcomes of COVID-19 in children compared with those of other sources of viral pneumonia diagnosed during the COVID-19 outbreak.Methods:Children with COVID-19 and viral pneumonia admitted to 20 hospitals were enrolled in this retrospective multi-center cohort study.A total of 64 children with COVID-19 were defined as the COVID-19 cohort,of which 40 children who developed pneumonia were defined as the COVID-19 pneumonia cohort.Another 284 children with pneumonia caused by other viruses were defined as the viral pneumonia cohort.The epidemiologic,clinical,and laboratory findings were compared by Kolmogorov-Smirnov test,t-test,Mann-Whitney U test and Contingency table method.Drug usage,immunotherapy,blood transfusion,and need for oxygen support were collected as the treatment indexes.Mortality,intensive care needs and symptomatic duration were collected as the outcome indicators.Results:Compared with the viral pneumonia cohort,children in the COVID-19 cohort were mostly exposed to family members confirmed to have COVID-19(53/64 vs.23/284),were of older median age(6.3 years vs.3.2 years),and had a higher proportion of ground-glass opacity(GGO)on computed tomography(18/40 vs.0/38,P<0.001).Children in the COVID-19 pneumonia cohort had a lower proportion of severe cases(1/40 vs.38/284,P=0.048),and lower cases with high fever(3/40 vs.167/284,P<0.001),requiring intensive care(1/40 vs.32/284,P<0.047)and with shorter symptomatic duration(median 5d vs.8d,P<0.001).The proportion of cases with evaluated inflammatory indicators,biochemical indicators related to organ or tissue damage,D-dimer and secondary bacterial infection were lower in the COVID-19 pneumonia cohort than those in the viral pneumonia cohort(P<0.05).No statistical differences were found in the duration of positive PCR results from pharyngeal swabs in 25 children with COVID-19 who received antiviral drugs(lopinavir-ritonavir,ribavirin,and arbidol)as compared with duration in 39 children without antiviral therapy(median 10d vs.9d,P=0.885).Conclusions:The symptoms and severity of COVID-19 pneumonia in children were no more severe than those in children with other viral pneumonia.Lopinavir-ritonavir,ribavirin and arbidol do not shorten the duration of positive PCR results from pharyngeal swabs in children with COVID-19.During the COVID-19 outbreak,attention also must be given to children with infection by other pathogens infection. 展开更多
关键词 CHILDREN Severe acute respiratory syndrome Coronavirus disease-2019 Viral pneumonia
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Kaposiform Hemangioendothelioma: Case Report and Literature Review 被引量:3
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作者 Gang Zhou Shuhua Yang +5 位作者 Xiu Nie Jingyuan Du Jin Li Jianxiang Liu Renhao Ze yu he 《Chinese Journal of Clinical Oncology》 CSCD 2007年第4期288-292,共5页
Kaposiform hemangioendothelioma (KHE) is a rare locally ag- gressive vascular tumor that mainly occurs in children and early adolescents. It is often associated with the
关键词 组织病理学 血管内皮瘤 骨头 病例报告
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Application of multiple Roux-en-Y hepaticojejunostomy reconstruction by formation of bile hilar duct lake in the operation of hilar cholangiocarcinoma 被引量:2
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作者 Xiao-Jun Yang Xiao-Hua Dong +5 位作者 Shi-Yong Chen Biao Wu yu he Bao-Long Dong Bing-Qiang Ma Peng Gao 《World Journal of Clinical Cases》 SCIE 2020年第1期68-75,共8页
BACKGROUND Hilar cholangiocarcinoma is the most common malignant tumor of the extrahepatic bile duct.Until now,radical resection has been the most effective method for the long-term survival of patients with the disea... BACKGROUND Hilar cholangiocarcinoma is the most common malignant tumor of the extrahepatic bile duct.Until now,radical resection has been the most effective method for the long-term survival of patients with the disease.However,many problems have emerged in the field of hepatobiliary surgery for a long time,including complex surgical procedures,low resection rate,and postoperative complications.We have adopted the“multiple Roux-en-Y hepaticojejunostomy reconstruction by formation of a bile duct lake”technique in the treatment of hilar cholangiocarcinoma since 2008,and obtained satisfactory short-and longterm results.AIM To examine the feasibility of the application of multiple Roux-en-Y hepaticojejunostomy reconstruction by formation of a bile duct lake in the operation of hilar cholangiocarcinoma METHODS A retrospective analysis was performed for the clinical data,surgical methods,and results of 76 patients with hilar cholangiocarcinoma who were treated with hilar bile duct lake-forming multiple Roux-en-Y hepaticojejunostomy reconstruction at Gansu Provincial Hospital.RESULTS In all 76 cases,the operation was successful and no operative death occurred.The mean(range)operation time was 215.4±53.5 min(124–678 min),and the amount of bleeding during the operation was 428.2±63.8 mL(240–2200 mL).The overall 1-year survival rate was 78.9%,and the 3-year survival rate was 32.8%.CONCLUSION The multiple Roux-en-Y hepaticojejunostomy reconstruction technique with formation of a bile duct lake is safe and effective for the surgical treatment of hilar cholangiocarcinoma. 展开更多
关键词 Hilar cholangiocarcinoma Bile duct lake HEPATICOJEJUNOSTOMY
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一种基于模型预测的火星返回推力器容错控制再分配方法 被引量:1
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作者 杨博 魏翔 +1 位作者 于贺 刘超凡 《宇航学报》 EI CAS CSCD 北大核心 2023年第2期243-253,共11页
针对火星返回上升器由于环境等因素造成的推力器故障导致的姿态控制系统失稳而难以安全返回的严重问题,提出基于模型预测的动态容错控制再分配方法。根据推力器动态特性建立上升器推力分配模型,对模型参数误差进行实时估计从而修正分配... 针对火星返回上升器由于环境等因素造成的推力器故障导致的姿态控制系统失稳而难以安全返回的严重问题,提出基于模型预测的动态容错控制再分配方法。根据推力器动态特性建立上升器推力分配模型,对模型参数误差进行实时估计从而修正分配模型,根据模型预测自适应推力再分配方法实施容错控制。同时,将推力器输出限制作为优化求解器的约束,并将推力器故障模型作为优化求解的约束域,实现最小化分配误差和最小化燃料消耗意义下的最优推力再分配。计算机仿真表明了该方法的可行性和实用性,获得了满意的结果,它能使推力器输出推力误差降低60%以上,姿态控制系统能在故障状态下3~5 s快速镇定。 展开更多
关键词 火星返回 容错技术 控制分配 模型预测
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