Detection of Lactobacillus acidophilus in Fermented Material by Real-time Fluorescent Quantitative PCR

The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template, and L. acidophilus in fer- mented material was conducted the quantitative determination by real-time quantitative PCR （RT-PCR）. Analysis on RT-PCR results shown that contents of L. aci- dophilus in the test sample reached 1.5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test. indicating that the established RT-PCR method could be aookued to the detection of L. acidophilus in fermented material.

高校管理人员胜任力因素分析与研究

高校作为教育人才培养的主阵地,承担培养高水平人才和科学研究的重要任务。高校管理人员作为高校管理活动的承担者,其管理水平和管理人员的胜任力水平在一定程度上影响高校人才培养质量。本研究聚焦于研究高校管理人员的胜任能力及其特征因素,并利用定性和定量相结合的分析方法,通过论文综述、问卷研究等方式筛选并识别出高校管理人员胜任力的关键因素,检验其有效性和可靠性,在此基础上,开展高校管理人员胜任力提升体系研究,为高校构建一流的管理体系并实施一流的管理提供支持。