目的:探讨罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的作用及相关机制。方法:通过免疫荧光和酶联免疫吸附检测罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的影响;采用生物信息学分析、定量聚合酶链式反应和双荧光素酶报告基因等实验筛选罗...目的:探讨罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的作用及相关机制。方法:通过免疫荧光和酶联免疫吸附检测罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的影响;采用生物信息学分析、定量聚合酶链式反应和双荧光素酶报告基因等实验筛选罗伊氏乳杆菌发挥作用的下游微小核糖核酸(microRNA,miRNA);通过双荧光素酶报告基因和Western blot等实验筛选miR-196a下游靶基因及其功能。结果:罗伊氏乳杆菌能够抑制脂多糖引起的肠上皮细胞炎症反应和凋亡,进一步分析发现罗伊氏乳杆菌通过上调肠上皮细胞miR-196a抑制细胞炎症和凋亡,miR-196a通过靶向抑制程序性细胞死亡因子4(programmed cell death 4,PDCD4)基因表达发挥对肠上皮细胞的保护作用。结论:罗伊氏乳杆菌通过miR-196a靶向抑制PDCD4基因表达,减缓肠上皮细胞炎症反应和凋亡。展开更多
The modern time of China is the most brilliant of the whole Chinese history,during which China was reduced from the feudal society to the semi-colonial and semi-feudal society and then a new China with the socialist s...The modern time of China is the most brilliant of the whole Chinese history,during which China was reduced from the feudal society to the semi-colonial and semi-feudal society and then a new China with the socialist system was founded after indomitable struggle.In such a context,the whole society experienced unsmooth development;while the music theories and music education soared with the invasion of capitalism.On the one hand,foreigners invaded China;on the other hand,they brought advanced music culture,promoting the music culture of China,which had stagnated for about two thousand years.This paper attempts to study the development path of music education in modern China from the perspective of development process.展开更多
阐述“双减”背景下初中英语单元作业的设计思路。以人教版(Go for it!)初中《英语》八年级(下)Unit 6 An old man tried to move the mountains为例,探究初中英语单元作业设计。提出教师要切合实际设计作业目标,注重创新、因材施教、...阐述“双减”背景下初中英语单元作业的设计思路。以人教版(Go for it!)初中《英语》八年级(下)Unit 6 An old man tried to move the mountains为例,探究初中英语单元作业设计。提出教师要切合实际设计作业目标,注重创新、因材施教、聚焦素养和提升能力。展开更多
分析目前的英语作业布置存在的问题。以人教版(Go for it!)初中《英语》八年级(下)Unit 6 An old man tried to move the mountains作业设计为例,探究指向思维品质培养的单元分层作业设计策略,阐述如何优化作业设计,对作业进行分层,从...分析目前的英语作业布置存在的问题。以人教版(Go for it!)初中《英语》八年级(下)Unit 6 An old man tried to move the mountains作业设计为例,探究指向思维品质培养的单元分层作业设计策略,阐述如何优化作业设计,对作业进行分层,从而帮助学生减轻作业负担,提升综合语言运用能力和英语学科核心素养。展开更多
文摘目的:探讨罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的作用及相关机制。方法:通过免疫荧光和酶联免疫吸附检测罗伊氏乳杆菌对肠上皮细胞炎症反应和凋亡的影响;采用生物信息学分析、定量聚合酶链式反应和双荧光素酶报告基因等实验筛选罗伊氏乳杆菌发挥作用的下游微小核糖核酸(microRNA,miRNA);通过双荧光素酶报告基因和Western blot等实验筛选miR-196a下游靶基因及其功能。结果:罗伊氏乳杆菌能够抑制脂多糖引起的肠上皮细胞炎症反应和凋亡,进一步分析发现罗伊氏乳杆菌通过上调肠上皮细胞miR-196a抑制细胞炎症和凋亡,miR-196a通过靶向抑制程序性细胞死亡因子4(programmed cell death 4,PDCD4)基因表达发挥对肠上皮细胞的保护作用。结论:罗伊氏乳杆菌通过miR-196a靶向抑制PDCD4基因表达,减缓肠上皮细胞炎症反应和凋亡。
基金one of the staged achievements of the philosophy and social science planning project“Research on the Art of Painting and Calligraphy in Chongqing during the Anti-Japanese War Period and Its Digital Applications”(No:2009XW04)
文摘The modern time of China is the most brilliant of the whole Chinese history,during which China was reduced from the feudal society to the semi-colonial and semi-feudal society and then a new China with the socialist system was founded after indomitable struggle.In such a context,the whole society experienced unsmooth development;while the music theories and music education soared with the invasion of capitalism.On the one hand,foreigners invaded China;on the other hand,they brought advanced music culture,promoting the music culture of China,which had stagnated for about two thousand years.This paper attempts to study the development path of music education in modern China from the perspective of development process.
文摘分析目前的英语作业布置存在的问题。以人教版(Go for it!)初中《英语》八年级(下)Unit 6 An old man tried to move the mountains作业设计为例,探究指向思维品质培养的单元分层作业设计策略,阐述如何优化作业设计,对作业进行分层,从而帮助学生减轻作业负担,提升综合语言运用能力和英语学科核心素养。
