目的了解鄂西南地区新生儿急性呼吸窘迫综合征(ARDS)的临床特点及转归。方法采用回顾性临床流行病学方法,根据新生儿ARDS蒙特勒诊断标准对2017年1~12月鄂西南地区17家二级或三级医院新生儿科/儿科收治的新生儿ARDS病例资料进行调查分析...目的了解鄂西南地区新生儿急性呼吸窘迫综合征(ARDS)的临床特点及转归。方法采用回顾性临床流行病学方法,根据新生儿ARDS蒙特勒诊断标准对2017年1~12月鄂西南地区17家二级或三级医院新生儿科/儿科收治的新生儿ARDS病例资料进行调查分析。结果所纳入的鄂西南地区17家医院2017年1年内共收治新生儿7150例,其中确诊新生儿ARDS 66例(0.92%),包括轻度23例(35%),中度28例(42%),重度15例(23%)。新生儿ARDS的主要原发疾病包括:围生期窒息(23例,35%)、肺炎(18例,27%)、败血症(12例,18%)、胎粪吸入综合征(10例,15%)。66例ARDS患儿中,产妇年龄≥35岁10例(15%),宫内窘迫30例(45%),1 min Apgar评分0~7分32例(49%),胎心监测异常24例(36%),羊水胎粪污染21例(32%)。脑室内出血是最常见的合并症(12例),其次为新生儿休克(9例)、动脉导管未闭(8例)。66例ARDS患儿在针对原发病治疗的基础上,均给予机械通气治疗。死亡10例,病死率为15%(10/66);治愈、好转56例(85%)。结论鄂西南地区新生儿ARDS以轻/中度为主;围生期窒息和感染可能是该地区新生儿ARDS的主要病因;脑室内出血是最常见的合并症;综合治疗后患儿存活率较高。展开更多
To investigate the low temperature on germination capacity （LTG） a double haploid rice （DH） population with 198 lines derived from anther culture of F1 hybrid with indica line Zhenshan 97B and a perennial japonica...To investigate the low temperature on germination capacity （LTG） a double haploid rice （DH） population with 198 lines derived from anther culture of F1 hybrid with indica line Zhenshan 97B and a perennial japonica line AAV002863 was used to construct a linkage map with 140 SSR markers, The germination rate in Zhenshan 97B and AAV002863 was 79,7% and 30,1%, while in DH population it ranged from 0 to 100% at 15℃ after 6 days, Quantitative trait loci （QTLs） controlling low temperature germinability were identified on chromosomes 3 and 10. The percentage of observed phenotypic variance attributed to qLTG-3 and qLTG-10 was 12.6% and 12.9%, respectively. Allele from Zhenshan 97B increased the LTG at qLTG-3 region, while allele from AAV002863 increased the LTG at qLTG-IO region. One pair of epistatic interaction was detected between loci on chromosomes 3 and 10. The main-effect of QTL on chromosome 10 was also involved in epistatic interaction.展开更多
To further improve upland rice varieties Huhan 3 and Huhan 7, seed samples were sent to outer space with two recoverable spaceships for approximately 1 and 5 d and were propagated for 7 and 5 generations, respectively...To further improve upland rice varieties Huhan 3 and Huhan 7, seed samples were sent to outer space with two recoverable spaceships for approximately 1 and 5 d and were propagated for 7 and 5 generations, respectively. Phenotypic analysis revealed that the morphological traits and the protein and amylose contents of grains changed. Characterization of genomic mutations by the gene-associated simple sequence repeat （SSR） and insertion-delete （InDel） markers indicated that the mutation pattern was very complex. Most of the mutations occurred at the 3＇- or 5＇-end of the fragments in the simple sequence repeat fragment. Reverse transcription-polymerase chain reaction （RT-PCR） assay showed that mutations in those parts of the SSR affected their gene expression, indicating that gene associated markers would be helpful to isolate functional genes. Field survey for breeding also revealed that more lines with high yield, high quality and drought-tolerance could be selected through aerospace breeding. The results indicate that aerospace mutagenesis resulted in molecular variation, as well as physiological and morphological changes for rice breeding.展开更多
To study the effects of long-term no-tillage direct seeding mode on rice yield and the soil physiochemical property in a rice-rapeseed rotation system, a comparative experiment with a water-saving and drought-resistan...To study the effects of long-term no-tillage direct seeding mode on rice yield and the soil physiochemical property in a rice-rapeseed rotation system, a comparative experiment with a water-saving and drought-resistance rice （WDR） variety and a double low rapeseed variety as materials was conducted under no-tillage direct seeding （NTDS） mode and conventional tillage direct seeding （CTDS） mode for four years, using the CTDS mode as the control. Compared with the CTDS mode, the actual rice yield of WDR decreased by 8.10% at the first year, whereas the plant height, spikelet number per panicle, spikelet fertility, 1000-grain weight, grain yield, actual yield, and harvest index increased with no-tillage years, which led to the actual yield increase by 6.49% at the fourth year. Correlation analysis showed that the panicle length was significantly related to the actual yield of WDR. Compared with the CTDS mode in terms of the soil properties, the pH value of the NTDS mode decreased every year, whereas the contents of soil organic matter and total N of the NTDS mode increased. In the 0-5 cm layer of the NTDS mode, the soil bulk decreased, whereas the contents of soil organic matter, total N, and available N increased. In the 5-20 cm layer of the NTDS mode, the available N and K decreased, whereas the soil bulk, contents of soil organic matter, and total N increased. In summary, the NTDS mode increased the rice yield, and could improve the paddy soil fertility of the top layer.展开更多
文摘目的了解鄂西南地区新生儿急性呼吸窘迫综合征(ARDS)的临床特点及转归。方法采用回顾性临床流行病学方法,根据新生儿ARDS蒙特勒诊断标准对2017年1~12月鄂西南地区17家二级或三级医院新生儿科/儿科收治的新生儿ARDS病例资料进行调查分析。结果所纳入的鄂西南地区17家医院2017年1年内共收治新生儿7150例,其中确诊新生儿ARDS 66例(0.92%),包括轻度23例(35%),中度28例(42%),重度15例(23%)。新生儿ARDS的主要原发疾病包括:围生期窒息(23例,35%)、肺炎(18例,27%)、败血症(12例,18%)、胎粪吸入综合征(10例,15%)。66例ARDS患儿中,产妇年龄≥35岁10例(15%),宫内窘迫30例(45%),1 min Apgar评分0~7分32例(49%),胎心监测异常24例(36%),羊水胎粪污染21例(32%)。脑室内出血是最常见的合并症(12例),其次为新生儿休克(9例)、动脉导管未闭(8例)。66例ARDS患儿在针对原发病治疗的基础上,均给予机械通气治疗。死亡10例,病死率为15%(10/66);治愈、好转56例(85%)。结论鄂西南地区新生儿ARDS以轻/中度为主;围生期窒息和感染可能是该地区新生儿ARDS的主要病因;脑室内出血是最常见的合并症;综合治疗后患儿存活率较高。
文摘To investigate the low temperature on germination capacity （LTG） a double haploid rice （DH） population with 198 lines derived from anther culture of F1 hybrid with indica line Zhenshan 97B and a perennial japonica line AAV002863 was used to construct a linkage map with 140 SSR markers, The germination rate in Zhenshan 97B and AAV002863 was 79,7% and 30,1%, while in DH population it ranged from 0 to 100% at 15℃ after 6 days, Quantitative trait loci （QTLs） controlling low temperature germinability were identified on chromosomes 3 and 10. The percentage of observed phenotypic variance attributed to qLTG-3 and qLTG-10 was 12.6% and 12.9%, respectively. Allele from Zhenshan 97B increased the LTG at qLTG-3 region, while allele from AAV002863 increased the LTG at qLTG-IO region. One pair of epistatic interaction was detected between loci on chromosomes 3 and 10. The main-effect of QTL on chromosome 10 was also involved in epistatic interaction.
基金supported by the National High-Technology Research and Development Program of China(Grant No.2010AA101805)National Key Technology R&D Program of China(Grant No.2013ZX08001-003)Shanghai Agricultural Science and Technology Key Projects,China(Grant No.2009-6-4-2)
文摘To further improve upland rice varieties Huhan 3 and Huhan 7, seed samples were sent to outer space with two recoverable spaceships for approximately 1 and 5 d and were propagated for 7 and 5 generations, respectively. Phenotypic analysis revealed that the morphological traits and the protein and amylose contents of grains changed. Characterization of genomic mutations by the gene-associated simple sequence repeat （SSR） and insertion-delete （InDel） markers indicated that the mutation pattern was very complex. Most of the mutations occurred at the 3＇- or 5＇-end of the fragments in the simple sequence repeat fragment. Reverse transcription-polymerase chain reaction （RT-PCR） assay showed that mutations in those parts of the SSR affected their gene expression, indicating that gene associated markers would be helpful to isolate functional genes. Field survey for breeding also revealed that more lines with high yield, high quality and drought-tolerance could be selected through aerospace breeding. The results indicate that aerospace mutagenesis resulted in molecular variation, as well as physiological and morphological changes for rice breeding.
基金supported by the Key Project of Developing Agriculture through Science and Technology of Shanghai Municipal Agricultural Commission,China(Grant No.2010-1-1)Shanghai Science and Technology Development Funds,China(Grant No.11QA1405900)the National High-Tech Research and Development Program of China(Grant No.2012AA101102)
文摘To study the effects of long-term no-tillage direct seeding mode on rice yield and the soil physiochemical property in a rice-rapeseed rotation system, a comparative experiment with a water-saving and drought-resistance rice （WDR） variety and a double low rapeseed variety as materials was conducted under no-tillage direct seeding （NTDS） mode and conventional tillage direct seeding （CTDS） mode for four years, using the CTDS mode as the control. Compared with the CTDS mode, the actual rice yield of WDR decreased by 8.10% at the first year, whereas the plant height, spikelet number per panicle, spikelet fertility, 1000-grain weight, grain yield, actual yield, and harvest index increased with no-tillage years, which led to the actual yield increase by 6.49% at the fourth year. Correlation analysis showed that the panicle length was significantly related to the actual yield of WDR. Compared with the CTDS mode in terms of the soil properties, the pH value of the NTDS mode decreased every year, whereas the contents of soil organic matter and total N of the NTDS mode increased. In the 0-5 cm layer of the NTDS mode, the soil bulk decreased, whereas the contents of soil organic matter, total N, and available N increased. In the 5-20 cm layer of the NTDS mode, the available N and K decreased, whereas the soil bulk, contents of soil organic matter, and total N increased. In summary, the NTDS mode increased the rice yield, and could improve the paddy soil fertility of the top layer.
