The effect of estrogen-mediated ubiquitin on cardiovascular diseases:a bioinformatics analysis

Objective:Using data mining tools,study the potential pathways of estrogen’s cardiovascular effects.Methods:The GeneExpression Omnibus database was used to download the relevant high-throughput microarray dataset GSE72180,which was then analyzed for differential genes using the GEO2R online analysis tool,gene function and pathway enrichment analysis using DAVID 6.8,protein interaction network analysis using the STRING database,and core network extraction using the MCODE algorithm.Results:A total of 131 differential genes were identified and enriched for gene function and signaling pathway analysis,which indicated that these genes were related with focal adhesion and the HIF-1 signaling pathway.MCODE algorithm analysis extracted 1 core sub-network of these genes to be related to ubiquitin protein transferase activity,protein polyubiquitination,protein ubiquitination involved in ubiquitin-dependent proteolytic metabolic processes,ligase activity,and clustering on ubiquitin-mediated protein hydrolysis signaling pathway.Conclusion:By using data mining tools,it is possible to identify how estrogen may influence the cardiovascular system by controlling the ubiquitination process.This information may be used as a reference for etiology and preventive studies of cardiovascular illnesses.

Effects of oxaliplatin, leucovorin and fluorouracil on serum tumor markers, VEGF, CRP and matrix metalloproteinases in patients with advanced esophageal cancer

Objective: To investigate the effects of oxaliplatin, leucovorin and fluorouracil on serum tumor markers, VEGF, CRP and matrix metalloproteinases in patients with advanced esophageal cancer. Methods: From March 2012 to March 2017 a total of 248 patients with advanced esophageal cancer were selected as the study subjects. According to random data table, they were divided into control group (n=123) and observation group (n=125) according to random data table. The control group was treated with cisplatin combined with fluorouracil, leucovorin chemotherapy, and patients in the observation group received oxaliplatin, leucovorin and fluorouracil chemotherapy, all patients were treated for 2 cycles. The changes of serum tumor markers, VEGF, CRP and matrix metalloproteinase levels in the two groups before and after treatment was compared. Results: Before treatment, there was no significant difference of the levels of serum CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 between the control group and the observation group. Compared with the group before treatment, the levels of CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 in the two groups were significantly lower. After treatment, the level of CA125, CA19-9, CEA, VEGF, CRP, MMP-2 and MMP-9 in the observation group was significantly lower than those of the control group. Conclusion:Oxaliplatin, leucovorin and fluorouracil chemotherapy can effectively reduce the levels of serum tumor markers, VEGF, CRP and matrix metalloproteinase in patients with advanced esophageal cancer, it has important clinical value.

Correlation of bisphenol A action on genes interfering with estrogen signaling pathways and the development of endometriosis

Bisphenol A(BPA),as an environmental endocrine disruptor,affects human health by interfering with hormone secretion in the body.Endometriosis(EMs)is an estrogendependent disease that is influenced by a variety of factors such as genetics,oxidative stress,immunity,and environment for its onset.In recent years,special attention has been paid to the influence of genetic polymorphisms on the pathogenesis of EMs.A search of the Comparative Toxicology Database revealed that BPA can act on a variety of different genes to induce disease development by regulating gene expression and that some genes play equally important roles in the development of EMs.Among them,we selected four genes(EGFR,ESR2,FOS,KRAS)in the estrogen signaling pathway,and now we review the mechanism of BPA acting on these four genes in different ways and the role of these four genes in the development of EMs,which will provide a reference for the subsequent study of the mechanism of BPA involvement in the development of EMs.

Clinical significance of DNAH14 gene in uterine corpus endometrial carcinoma based on bioinformatics

Background:To analyze the effect of DNAH14 gene on the development of endometrial carcinoma and its clinical significance by using tumor comprehensive database.Methods:First,the aberrant expression of the DNAH14 gene in multiple tumor tissues was obtained in the gene expression profiling interactive analysis.Then clinicopathological data were obtained at the cancer genome atlas.The expression and prognostic value of the DNAH14 gene in endometrial cancer were assessed.Subsequently,Kaplan-Meier mapping instrument analysis was used to assess the significance of DNAH14 mRNA expression and DNAH14 gene mutations in the prognosis of uterine corpus endometrial carcinoma patients.The relationship between immune infiltrating cells and uterine corpus endometrial carcinoma survival was analyzed using the Tumor Immune Estimation Resource database.Finally,the Mu Target database was used to analyze and identify genes that exhibit altered expression in samples containing mutated genes,then the Database for Annotation,Visualization and Integrated Discovery was used to explore the biological processes closely associated with DNAH14 mutations and enriched signaling pathways,and the Search Tool for Recurring Instances of Neighbouring Genes database to construct protein interaction networks that may interact with DNAH14 and explore their potential mechanisms of action.Results:The overall survival rate and relapse-free survival rate in patients with high expression of DNAH14 were significantly lower than those with low expression and there were significant differences in clinical stage,age,menopausal status and histological types(P<0.05).DNAH14 gene mutation exists in patients with endometrial carcinoma,indicating a good prognosis.Correlation analysis showed that DNAH14 gene mutation might play a role through proteoglycan and Wnt,T cell receptor,toll-like receptor signal pathway in cancer.In addition,DNAH14 mutation is related to the level of immune infiltration;protein interaction analysis showed that DCTN3,HAP1,ACTR1,DNAH12,ACTR1B and other proteins interact with DNAH14.Conclusion:DNAH14 is highly expressed in endometrial carcinoma and its high expression is related to the poor prognosis of the patients.DNAH14 gene mutation in patients with endometrial carcinoma may affect the level of immune infiltration and improve prognosis through proteoglycan,Wnt,T cell receptor and toll-like receptor signal pathway in cancer.DNAH14 may be an index to judge the prognosis of patients with endometrial carcinoma.