Effects of Lactobacillus plantarum on gut barrier function in experimental obstructive jaundice

AIM:To investigate the mechanisms of Lactobacillus plantarum(L.plantarum)action on gut barrier in preoperative and postoperative experimental obstructive jaundice in rats.METHODS:Forty rats were randomly divided into groups of sham-operation,bile duct ligation(BDL),BDL +L.plantarum,BDL+internal biliary drainage(IBD),and BDL+IBD+L.plantarum.Ten days after L.plantarum administration,blood and ileal samples were collected from the rats for morphological examination,and intestinal barrier function,liver function,intestinal oxidative stress and protein kinase C(PKC)activity measurement.The distribution and expression of the PKC and tight junction(TJ)proteins,such as occludin,zonula occludens-1,claudin-1,claudin-4,junction adhesion molecule-A and F-actin,were examined by confocal laser scanning microscopy,immunohistochemistry,Western blotting,real-time fluorescent quantitative polymerase chain reaction assay.RESULTS:L.plantarum administration substantially restored gut barrier,decreased enterocyte apoptosis,improved intestinal oxidative stress,promoted the activity and expression of protein kinase(BDL vs BDL+L.plantarum,0.295±0.007 vs 0.349±0.003,P<0.05;BDL+IBD vs BDL+IBD+L.plantarum,0.407±0.046 vs 0.465±0.135,P<0.05),and particularly enhanced the expression and phosphorylation of TJ proteins in the experimental obstructive jaundice(BDL vs BDL+L.plantarum,0.266±0.118 vs 0.326±0.009,P<0.05).The protective effect of L.plantarum was more prominent after internal biliary drainage(BDL+IBD vs BDL +IBD+L.plantarum,0.415±0.105 vs 0.494±0.145,P<0.05).CONCLUSION:L.plantarum can decrease intestinal epithelial cell apoptosis,reduce oxidative stress,and prevent TJ disruption in biliary obstruction by activating the PKC pathway.

Comparative analysis of the clinical outcomes between wavefront-guided and conventional femtosecond LASIK in myopia and myopia astigmatism

AIM:To compare the clinical outcomes of wavefront guided femtosecond LASIK(WFG LASIK)and conventional femtosecond LASIK(NWFG LASIK)in eyes with myopia and myopia astigmatism.METHODS:This was a retrospective,nonrandomized,comparative investigation enrolling 236 eyes of 122 patients(18-50 y)with low&moderate and high myopia.The WFG group including 97 eyes(50 patients)undergone WFG LASIK and the NWFG group including 139 eyes(72 patients)undergone conventional LASIK.Mean efficacy index,high order aberrations(HOAs),pupil size and the quality of visual questionnaire were evaluated 6 mo postoperatively.RESULTS:There is no difference between WFG group(-0.054±0.049 in logMAR)and NWFG group(-0.040±0.056)in uncorrected distance visual acuity(UDVA)postoperatively.The myopia astigmatism is higher in WFG group than that in NWFG group(P<0.05).However,the mean efficacy index(MEI)in the WFG group(1.09±0.106)is better than that in the NWFG group(1.036±0.124;P<0.001).Increased HOAs were observed in NWFG group(0.30±0.196)than that in WFG group(0.146±0.188;P<0.001).The pupil size is larger in WFG group(5.15±0.76 mm)than that in NWFG group(4.32±0.52 mm).The patients are satisfied with the clinical surgery,yet WFG group showed better visual quality using the questionnaire survey.Meanwhile,high myopia would result in worse MEI,HOAs and visual quality than low&moderate myopia.CONCLUSION:WFG and NWFG FS-LASIK are both effective and safe procedures to correct low&moderate and high myopia,but WFG FS-LASIK gives a better postoperative MEI,aberrometric control and predictable outcome.Meanwhile,WFG FS-LASIK is better than NWFG FS-LASIK in correction of myopia astigmatism.Low&moderate myopia allow better clinical outcomes than high myopia using any surgical method.

冈下肌腱固定术治疗肩关节前向不稳的疗效分析

目的探讨冈下肌腱固定术治疗伴有对吻性损伤的肩关节前向不稳患者的临床可行性。方法选取2013年1月-2017年1月于贵州医科大学附属医院收治的80例伴有对吻性损伤的肩关节前向不稳患者,依据治疗方法将这些患者分为关节镜下盂唇修补术联合冈下肌腱固定术组(联合手术组)和单独关节镜下盂唇修补术组(单独手术组),对两组患者的肩关节中立位外旋、内旋活动度、OSIS评分、ROWE评分及临床疗效进行分析。结果联合手术组术后OSIS评分低于单独手术组(P<0.05),术后ROWE评分高于单独手术组(P <0.05),联合手术组治疗的优良率高于单独手术组(P <0.05)。结论冈下肌腱固定术在伴有对吻性损伤的肩关节前向不稳患者治疗中的临床可行性高,值得临床推广使用。

Enhancing security of NVM-based main memory with dynamic Feistel network mapping

As promising alternatives in building future main memory systems, emerging non-volatile memory(NVM) technologies can increase memory capacity in a cost-effective and power-efficient way. However, NVM is facing security threats due to its limited write endurance: a malicious adversary can wear out the cells and cause the NVM system to fail quickly. To address this issue, several wear-leveling schemes have been proposed to evenly distribute write traffic in a security-aware manner. In this study, we present a new type of timing attack, remapping timing attack(RTA), based on information leakage from the remapping latency difference in NVM. Our analysis and experimental results show that RTA can cause three of the latest wear-leveling schemes(i.e., region-based start-gap,security refresh, and multi-way wear leveling) to lose their effectiveness in several days(even minutes), causing failure of NVM. To defend against such an attack, we further propose a novel wear-leveling scheme called the ‘security region-based start-gap(security RBSG)', which is a two-stage strategy using a dynamic Feistel network to enhance the simple start-gap wear leveling with level-adjustable security assurance. The theoretical analysis and evaluation results show that the proposed security RBSG not only performs well when facing traditional malicious attacks, but also better defends against RTA.