BACKGROUND: The results are conflicting in detecting P-glycoprotein (P-gp) in pancreatic cancer. The aim of this study was to detect the expression of multidrug resis- tant gene 1 ( MDR1) in pancreatic cancer cell lin...BACKGROUND: The results are conflicting in detecting P-glycoprotein (P-gp) in pancreatic cancer. The aim of this study was to detect the expression of multidrug resis- tant gene 1 ( MDR1) in pancreatic cancer cell lines. METHODS: MDR1 mRNA and P-gp were detected by re- verse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical assay (IHC) in three pancreatic cancer cell lines SW1990, CAPAN-1 and P3. P-gp func- tions were evaluated through the rhodamine extrusion test. RESULTS: Two of the three cell lines expressed MDR1 po- sitively at different levels. The rhodamine extrusion test showed that the percentage of positive cells in MDR(+) cells was significantly lower than that in MDR1 (-) cells. The results of IHC, RT-PCR and the rhodamine extrusion test were consistent with each other. CONCLUSION: All of these methods are reliable in the de- tection of MDR1 in pancreatic cancer tissue, thus providing a guide for clinical chemotherapy of pancreatic cancer.展开更多
基金This study was supported by a grant from the Excellent Young TeachersFoundation of the Ministry of Education, China.
文摘BACKGROUND: The results are conflicting in detecting P-glycoprotein (P-gp) in pancreatic cancer. The aim of this study was to detect the expression of multidrug resis- tant gene 1 ( MDR1) in pancreatic cancer cell lines. METHODS: MDR1 mRNA and P-gp were detected by re- verse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical assay (IHC) in three pancreatic cancer cell lines SW1990, CAPAN-1 and P3. P-gp func- tions were evaluated through the rhodamine extrusion test. RESULTS: Two of the three cell lines expressed MDR1 po- sitively at different levels. The rhodamine extrusion test showed that the percentage of positive cells in MDR(+) cells was significantly lower than that in MDR1 (-) cells. The results of IHC, RT-PCR and the rhodamine extrusion test were consistent with each other. CONCLUSION: All of these methods are reliable in the de- tection of MDR1 in pancreatic cancer tissue, thus providing a guide for clinical chemotherapy of pancreatic cancer.
