Excessive dexamethasone (Dex) administrated into pregnant mice during critical periods of palatal development can produce a high incidence of cleft palate. Its mechanisms remain unknown. Vitamin B12 has been shown to ...Excessive dexamethasone (Dex) administrated into pregnant mice during critical periods of palatal development can produce a high incidence of cleft palate. Its mechanisms remain unknown. Vitamin B12 has been shown to antagonize the tera- togenic effects of Dex, which, however, remains controversial. In this study, we investigated the effects of Dex and vitamin B12 on murine embryonic palatal shelf fusion using organ culture of murine embryonic shelves. The explanted palatal shelves on em- bryonic day 14 (E14) were cultured for 24, 48, 72 or 96 h in different concentrations of Dex and/or vitamin B12. The palatal shelves were examined histologically for the morphological alterations on the medial edge epithelium (MEE) and fusion rates among different groups. It was found that the palatal shelves were not fused at 72 h or less of culture in Dex group, while they were completely fused in the control and vitamin B12-treated groups at 72 and 96 h, respectively. The MEE still existed and proliferated. In Dex+vitamin B12 group the palatal shelves were fused at each time point in a similar rate to controls. These results may suggest that Dex causes teratogenesis of murine embryonic palatal shelves and vitamin B12 prevents the teratogenic effect of Dex on palatogenesis on murine embryos in vitro.展开更多
Aim To investigate the effect of DAPT （γ-secretase inhibitor） on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of t...Aim To investigate the effect of DAPT （γ-secretase inhibitor） on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma. Methodology Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using Indigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence （IF） were employed to determine the intracellular expression levels. Results DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G0-G1 cell cycle arrest and apoptosis, The mRNA levels of Hairy/Enhancer of Split-1 （Hes-1）, a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells. Conclusion DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch- 1 and Caspase-3.展开更多
The instability of p-channel low-temperature polycrystalline silicon thin film transistors(poly-Si TFTs)is investigated under negative gate bias stress(NBS)in this work.Firstly,a series of negative bias stress experim...The instability of p-channel low-temperature polycrystalline silicon thin film transistors(poly-Si TFTs)is investigated under negative gate bias stress(NBS)in this work.Firstly,a series of negative bias stress experiments is performed,the significant degradation behaviors in current-voltage characteristics are observed.As the stress voltage decreases from-25 V to-37 V,the threshold voltage and the sub-threshold swing each show a continuous shift,which is induced by gate oxide trapped charges or interface state.Furthermore,low frequency noise(LFN)values in poly-Si TFTs are measured before and after negative bias stress.The flat-band voltage spectral density is extracted,and the trap concentration located near the Si/SiO2 interface is also calculated.Finally,the degradation mechanism is discussed based on the current-voltage and LFN results in poly-Si TFTs under NBS,finding out that Si-OH bonds may be broken and form Si*and negative charge OH-under negative bias stress,which is demonstrated by the proposed negative charge generation model.展开更多
BACKGROUND Currently,the standard surgical procedure for right colon cancer is complete mesocolic excision.Whether preventive extended lymph node dissection for colon cancer located in the hepatic flexure or right tra...BACKGROUND Currently,the standard surgical procedure for right colon cancer is complete mesocolic excision.Whether preventive extended lymph node dissection for colon cancer located in the hepatic flexure or right transverse colon should be performed remains controversial because the safety and effectiveness of the operation have not been proven,and infrapyloric lymph nodes(No.206)and lymph nodes in the greater curvature of the stomach(No.204)have not been strictly defined and distinguished as surgical indicators in previous studies.AIM To analyze the metastatic status of infrapyloric lymph nodes and lymph nodes of the greater curvature of the stomach and perioperative complications and systematically evaluate the feasibility and safety of laparoscopic extended right colectomy using prospective data collected retrospectively.METHODS The study was a clinical study.Twenty patients with colon cancer who underwent laparoscopic extended right colon resection in our hospital from June 2020 to May 2021 were included.RESULTS Among the patients who underwent extended right colon resection,there were no intraoperative complications or conversion to laparotomy;2 patients had gastrocolic ligament lymph node metastasis,and 5 patients had postoperative complications.The patients with postoperative complications received conservative treatment.CONCLUSION Laparoscopic extended right colon resection is safe.However,malignant tumors located in the liver flexure or the right-side transverse colon are more likely to metastasize to the gastrocolic ligament lymph nodes,and notably,the incidence of gastroparesis was high.The number of patients was small,and the follow-up time was short.It is necessary to further increase the sample size to evaluate the No.204 and No.206 lymph node metastasis rates and the long-term survival impact.展开更多
BACKGROUND Chylous ascites following right colectomy has a high incidence which is a critical challenge.At present,there are few studies on the factors affecting chylous ascites after right colectomy and especially af...BACKGROUND Chylous ascites following right colectomy has a high incidence which is a critical challenge.At present,there are few studies on the factors affecting chylous ascites after right colectomy and especially after D3 Lymphadenectomy.A predictive model for chylous ascites has not yet been established.Therefore,we created the first nomogram to predict the incidence of chylous ascites after right hemicolectomy.AIM To analyze the risk factors for chylous ascites after right colectomy and establish a nomogram to predict the incidence of chylous ascites.METHODS We retrospectively collected patients who underwent right hemicolectomy between January 2012 and May 2021 and were pathologically diagnosed with cancer.Multivariate logistic regression was used to analyze the influencing factors of chylous ascites and a nomogram was established.The predictive ability was assessed by the area under the receiver operating characteristic(ROC)curve.RESULTS Operative time,the type of operation(standard or extended),the number of lymph nodes retrieved,and somatostatin administration were considered important risk factors.Multivariate logistic regression and nomograms can be used to accurately predict whether chylous ascites occurs.The area under the ROC curve of the model is 0.770.The C-statistic of this model is 0.770 which indicates that it has a relatively moderate ability to predict the risk of chylous ascites.CONCLUSION We found a novel set of risk factors,created a nomogram,and validated it.The nomogram had a relatively accurate forecasting ability for chylous ascites after right hemicolectomy and can be used as a reference for risk assessment of chylous ascites and whether to prevent it after surgery.展开更多
Consider the KdV equations with the external periodic excitation u t+uu x+u xxx +γu=f(t) , with f(t+T)=f(t). Prove the existence of attractors of resulting discrete semigroup ｛S(τ+mT)｝ m∈N .
Soil abundant taxa diversity positively related to multifunctionality under Hg stress.•Microbial network complexity of soil abundant taxa supported the strength of SBF.•Stochastic assembly of soil abundant subcommunit...Soil abundant taxa diversity positively related to multifunctionality under Hg stress.•Microbial network complexity of soil abundant taxa supported the strength of SBF.•Stochastic assembly of soil abundant subcommunity supported the strength of SBF.•Stochastic ratio was the most important predictor for the strength of SBF.It is known that soil microbial communities are intricately linked to multiple ecosystem functions and can maintain the relationship between soil biodiversity and multifunctionality(SBF)under environmental stresses.However,the relative contributions and driving forces of abundant and rare taxa within the communities in maintaining soil biodiversity-multifunctionality relationship under pollution stresses are still unclear.Here,we conducted microcosm experiments to estimate the importance of soil abundant and rare taxa in predicting these relationships under heavy metal mercury(Hg)stress in paired paddy and upland fields.The results revealed that the diversity of abundant taxa,rather than rare taxa,was positively related to multifunctionality,with the abundant subcommunity tending to maintain a larger proportion of soil functions including chitin degradation,protein degradation,and phosphorus mineralization.Soil multitrophic network complexity consisting of abundant species showed positive correlations with biodiversity and multifunctionality,and supported the strength of SBF within a network complexity range.Stochastic assembly processes of the abundant subcommunity were positively correlated with the strength of SBF,although stochastic processes decreased the biodiversity and the multifunctionality,respectively.After simultaneously accounting for multiple factors on the strength of SBF,we found that the stochastic community assembly ratio of abundant taxa was the most important predictor for SBF strength under Hg stress.Our results highlight the importance of abundant taxa in supporting soil multifunctionality,and elucidate the linkages between community assembly,network complexity and SBF relationship under environmental stresses.展开更多
The responses of soil ammonia-oxidizing archaea（AOA） and ammonia-oxidizing bacteria（AOB） to mercury（Hg） stress were investigated through a short-term incubation experiment.Treated with four different concentrati...The responses of soil ammonia-oxidizing archaea（AOA） and ammonia-oxidizing bacteria（AOB） to mercury（Hg） stress were investigated through a short-term incubation experiment.Treated with four different concentrations of Hg（CK,Hg25,Hg50,and Hg100,denoting 0,25,50,and 100 mg Hg/kg dry soil,respectively）,samples were harvested after 3,7,and 28 day incubation.Results showed that the soil potential nitrification rate（PNR） was significantly inhibited by Hg stress during the incubation.However,lower abundances of AOA（the highest in CK： 9.20 × 10~7 copies/g dry soil; the lowest in Hg50： 2.68 × 10~7 copies/g dry soil） and AOB（the highest in CK： 2.68 × 10~7 copies/g dry soil; the lowest in Hg50：7.49 × 10~6 copies/g dry soil） were observed only at day 28 of incubation（P 〈 0.05）.Moreover,only the community structure of soil AOB obviously shifted under Hg stress as seen through DGGE profiles,which revealed that 2-3 distinct AOB bands emerged in the Hg treatments at day 28.In summary,soil PNR might be a very useful parameter to assess acute Hg stress on soil ecosystems,and the community structure of soil AOB might be a realistic biological indicator for the assessment of heavy metal stress on soil ecosystems in the future.展开更多
基金Project (No. 30530730) supported by the National Natural ScienceFoundation of China
文摘Excessive dexamethasone (Dex) administrated into pregnant mice during critical periods of palatal development can produce a high incidence of cleft palate. Its mechanisms remain unknown. Vitamin B12 has been shown to antagonize the tera- togenic effects of Dex, which, however, remains controversial. In this study, we investigated the effects of Dex and vitamin B12 on murine embryonic palatal shelf fusion using organ culture of murine embryonic shelves. The explanted palatal shelves on em- bryonic day 14 (E14) were cultured for 24, 48, 72 or 96 h in different concentrations of Dex and/or vitamin B12. The palatal shelves were examined histologically for the morphological alterations on the medial edge epithelium (MEE) and fusion rates among different groups. It was found that the palatal shelves were not fused at 72 h or less of culture in Dex group, while they were completely fused in the control and vitamin B12-treated groups at 72 and 96 h, respectively. The MEE still existed and proliferated. In Dex+vitamin B12 group the palatal shelves were fused at each time point in a similar rate to controls. These results may suggest that Dex causes teratogenesis of murine embryonic palatal shelves and vitamin B12 prevents the teratogenic effect of Dex on palatogenesis on murine embryos in vitro.
基金funded by the National Natural Science Foundation of China(30801304)Specialized Research Fund for the Doctoral Program of Higher Education(20070610062)+1 种基金Opening Funding of the State Key Laboratory of Oral Diseases, Sichuan University(SKLOD011)the Applied Fundarmental Project of Sichuan Province(2008 JY0028-2)
文摘Aim To investigate the effect of DAPT （γ-secretase inhibitor） on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma. Methodology Human tongue carcinoma Tca8113 cells were cultured with DAPT. Cell growth was determined using Indigotic Reduction method. The cell cycle and apoptosis were analyzed by flow cytometry. Real-time PCR and Immuno-Fluorescence （IF） were employed to determine the intracellular expression levels. Results DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G0-G1 cell cycle arrest and apoptosis, The mRNA levels of Hairy/Enhancer of Split-1 （Hes-1）, a target of Notch activation, were reduced by DAPT in a dose-dependent manner. Coincident with this observation, DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells. Conclusion DAPT may have a therapeutic value for human tongue carcinoma. Moreover, the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch- 1 and Caspase-3.
基金Project supported by the National Natural Science Foundation of China(Grant No.61574048)the Pearl River Science and Technology Nova Program of Guangzhou City,China(Grant No.201710010172)+2 种基金the International Science and Technology Cooperation Program of Guangzhou City(Grant No.201807010006)the International Cooperation Program of Guangdong Province,China(Grant No.2018A050506044)the Opening Fund of Key Laboratory of Silicon Device Technology,China(Grant No.KLSDTJJ2018-6)
文摘The instability of p-channel low-temperature polycrystalline silicon thin film transistors(poly-Si TFTs)is investigated under negative gate bias stress(NBS)in this work.Firstly,a series of negative bias stress experiments is performed,the significant degradation behaviors in current-voltage characteristics are observed.As the stress voltage decreases from-25 V to-37 V,the threshold voltage and the sub-threshold swing each show a continuous shift,which is induced by gate oxide trapped charges or interface state.Furthermore,low frequency noise(LFN)values in poly-Si TFTs are measured before and after negative bias stress.The flat-band voltage spectral density is extracted,and the trap concentration located near the Si/SiO2 interface is also calculated.Finally,the degradation mechanism is discussed based on the current-voltage and LFN results in poly-Si TFTs under NBS,finding out that Si-OH bonds may be broken and form Si*and negative charge OH-under negative bias stress,which is demonstrated by the proposed negative charge generation model.
基金Supported by the Malignant Tumor Clinical Medicine Research Center,Quanzhou City,Fujian Province,China,No.2020N090s.
文摘BACKGROUND Currently,the standard surgical procedure for right colon cancer is complete mesocolic excision.Whether preventive extended lymph node dissection for colon cancer located in the hepatic flexure or right transverse colon should be performed remains controversial because the safety and effectiveness of the operation have not been proven,and infrapyloric lymph nodes(No.206)and lymph nodes in the greater curvature of the stomach(No.204)have not been strictly defined and distinguished as surgical indicators in previous studies.AIM To analyze the metastatic status of infrapyloric lymph nodes and lymph nodes of the greater curvature of the stomach and perioperative complications and systematically evaluate the feasibility and safety of laparoscopic extended right colectomy using prospective data collected retrospectively.METHODS The study was a clinical study.Twenty patients with colon cancer who underwent laparoscopic extended right colon resection in our hospital from June 2020 to May 2021 were included.RESULTS Among the patients who underwent extended right colon resection,there were no intraoperative complications or conversion to laparotomy;2 patients had gastrocolic ligament lymph node metastasis,and 5 patients had postoperative complications.The patients with postoperative complications received conservative treatment.CONCLUSION Laparoscopic extended right colon resection is safe.However,malignant tumors located in the liver flexure or the right-side transverse colon are more likely to metastasize to the gastrocolic ligament lymph nodes,and notably,the incidence of gastroparesis was high.The number of patients was small,and the follow-up time was short.It is necessary to further increase the sample size to evaluate the No.204 and No.206 lymph node metastasis rates and the long-term survival impact.
基金Malignant Tumor Clinical Medicine Research Center,Quanzhou City,Fujian Province,China,No.2020N090s.
文摘BACKGROUND Chylous ascites following right colectomy has a high incidence which is a critical challenge.At present,there are few studies on the factors affecting chylous ascites after right colectomy and especially after D3 Lymphadenectomy.A predictive model for chylous ascites has not yet been established.Therefore,we created the first nomogram to predict the incidence of chylous ascites after right hemicolectomy.AIM To analyze the risk factors for chylous ascites after right colectomy and establish a nomogram to predict the incidence of chylous ascites.METHODS We retrospectively collected patients who underwent right hemicolectomy between January 2012 and May 2021 and were pathologically diagnosed with cancer.Multivariate logistic regression was used to analyze the influencing factors of chylous ascites and a nomogram was established.The predictive ability was assessed by the area under the receiver operating characteristic(ROC)curve.RESULTS Operative time,the type of operation(standard or extended),the number of lymph nodes retrieved,and somatostatin administration were considered important risk factors.Multivariate logistic regression and nomograms can be used to accurately predict whether chylous ascites occurs.The area under the ROC curve of the model is 0.770.The C-statistic of this model is 0.770 which indicates that it has a relatively moderate ability to predict the risk of chylous ascites.CONCLUSION We found a novel set of risk factors,created a nomogram,and validated it.The nomogram had a relatively accurate forecasting ability for chylous ascites after right hemicolectomy and can be used as a reference for risk assessment of chylous ascites and whether to prevent it after surgery.
文摘Consider the KdV equations with the external periodic excitation u t+uu x+u xxx +γu=f(t) , with f(t+T)=f(t). Prove the existence of attractors of resulting discrete semigroup ｛S(τ+mT)｝ m∈N .
基金financially supported by the National Natural Science Foundation of China(42177022 and 41877120)Natural Science Foundation of Hubei Province,China(2020CFA013).
文摘Soil abundant taxa diversity positively related to multifunctionality under Hg stress.•Microbial network complexity of soil abundant taxa supported the strength of SBF.•Stochastic assembly of soil abundant subcommunity supported the strength of SBF.•Stochastic ratio was the most important predictor for the strength of SBF.It is known that soil microbial communities are intricately linked to multiple ecosystem functions and can maintain the relationship between soil biodiversity and multifunctionality(SBF)under environmental stresses.However,the relative contributions and driving forces of abundant and rare taxa within the communities in maintaining soil biodiversity-multifunctionality relationship under pollution stresses are still unclear.Here,we conducted microcosm experiments to estimate the importance of soil abundant and rare taxa in predicting these relationships under heavy metal mercury(Hg)stress in paired paddy and upland fields.The results revealed that the diversity of abundant taxa,rather than rare taxa,was positively related to multifunctionality,with the abundant subcommunity tending to maintain a larger proportion of soil functions including chitin degradation,protein degradation,and phosphorus mineralization.Soil multitrophic network complexity consisting of abundant species showed positive correlations with biodiversity and multifunctionality,and supported the strength of SBF within a network complexity range.Stochastic assembly processes of the abundant subcommunity were positively correlated with the strength of SBF,although stochastic processes decreased the biodiversity and the multifunctionality,respectively.After simultaneously accounting for multiple factors on the strength of SBF,we found that the stochastic community assembly ratio of abundant taxa was the most important predictor for SBF strength under Hg stress.Our results highlight the importance of abundant taxa in supporting soil multifunctionality,and elucidate the linkages between community assembly,network complexity and SBF relationship under environmental stresses.
基金supported by the National Key Research and Development Program of China(2021YFD1901205)the National Natural Science Foundation of China(42177022 and 32071595)+3 种基金the Fundamental Research Funds for the Central Universities(2662023PY010)the Spanish Ministry of Science and Innovation(PID2020-115813RA-I00)the Fondo Europeo de Desarrollo Regional(FEDER)the Consejería de Transformación Económica,Industria,Conocimiento y Universidades of the Junta de Andalucía(FEDER Andalucía 2014–2020 Objetivo temático“01-Refuerzo de la investigación,el desarrollo tecnológico y la innovación”)(P20_00879(ANDABIOMA))。
基金supported by the Natural Science Foundation of China (Nos.41371477,41071163)the National Key Technology R&D Program (No.2014BAD14B01)the Fundamental Research Funds for the Central Universities (No.XDJK2014B047)
文摘The responses of soil ammonia-oxidizing archaea（AOA） and ammonia-oxidizing bacteria（AOB） to mercury（Hg） stress were investigated through a short-term incubation experiment.Treated with four different concentrations of Hg（CK,Hg25,Hg50,and Hg100,denoting 0,25,50,and 100 mg Hg/kg dry soil,respectively）,samples were harvested after 3,7,and 28 day incubation.Results showed that the soil potential nitrification rate（PNR） was significantly inhibited by Hg stress during the incubation.However,lower abundances of AOA（the highest in CK： 9.20 × 10~7 copies/g dry soil; the lowest in Hg50： 2.68 × 10~7 copies/g dry soil） and AOB（the highest in CK： 2.68 × 10~7 copies/g dry soil; the lowest in Hg50：7.49 × 10~6 copies/g dry soil） were observed only at day 28 of incubation（P 〈 0.05）.Moreover,only the community structure of soil AOB obviously shifted under Hg stress as seen through DGGE profiles,which revealed that 2-3 distinct AOB bands emerged in the Hg treatments at day 28.In summary,soil PNR might be a very useful parameter to assess acute Hg stress on soil ecosystems,and the community structure of soil AOB might be a realistic biological indicator for the assessment of heavy metal stress on soil ecosystems in the future.
