Gene Repair of iPSC Line with GARS (G294R) Mutation of CMT2D Disease by CRISPR/Cas9

Objective Charcot-Marie-Tooth disease(CMT)severely affects patient activity,and may cause disability.However,no clinical treatment is available to reverse the disease course.The combination of CRISPR/Cas9 and iPSCs may have therapeutic potential against nervous diseases,such as CMT.Methods In the present study,the skin fibroblasts of CMT type 2D(CMT2D)patients with the c.880G>A heterozygous nucleotide mutation in the GARS gene were reprogrammed into iPSCs using three plasmids(pCXLE-hSK,pCXLE-hUL and pCXLE-hOCT3/4-shp5-F).Then,CRISPR/Cas9 technology was used to repair the mutated gene sites at the iPSC level.Results An iPSC line derived from the GARS(G294R)family with fibular atrophy was successfully induced,and the mutated gene loci were repaired at the iPSC level using CRISPR/Cas9 technology.These findings lay the foundation for future research on drug screening and cell therapy.Conclusion iPSCs can differentiate into different cell types,and originate from autologous cells.Therefore,they are promising for the development of autologous cell therapies for degenerative diseases.The combination of CRISPR/Cas9 and iPSCs may open a new avenue for the treatment of nervous diseases,such as CMT.

Enhanced photoluminescence of monolayer MoS_(2)on stepped gold structure

Different Mo S_(2)/Au heterostructures can play an important role in tuning the photoluminescence(PL)and optoelectrical properties of monolayer Mo S_(2).Previous studies of PL of Mo S_(2)/Au heterostructures were mainly limited to the PL enhancement by using different Au nanostructures and PL quenching of monolayer Mo S_(2)on flat Au surfaces.Here,we demonstrate the enhanced excitonic PL emissions of monolayer Mo S_(2)/Au heterostructures on Si/Si O_(2)substrates.By transferring the continuous monolayer Mo S_(2)onto a stepped Au structure consisting of 60-nm and 100-nm Au films,the Mo S_(2)/Au-60 and Mo S_(2)/Au-100 heterostructures exhibit enhanced PL emissions,each with a blue-shifted PL peak in comparison with the Mo S_(2)/Si O_(2).Furthermore,the PL intensity of Mo S_(2)/Au-60 is about twice larger than that of Mo S_(2)/Au-100.The different enhanced excitonic PL emissions in Mo S_(2)/Au heterostructures can be attributed to the different charge transfer effects modified by the stepped Au structure.This work may provide an insight into the excitonic PL and charge transfer effect of Mo S_(2)on Au film and yield novel phenomena in Mo S_(2)/Au heterostructures for further study of PL tuning and optoelectrical properties.

A Local Limit Theorem for Solutions of BSDEs with Mao's non-Lipschitz Generator

This paper establishes a local limit theorem for solutions of backward stochastic differential equations with Mao＇s non-Lipschitz generator, which is similar to the limit theorem obtained by [3] under the Lipschitz assumption.