宫颈鳞癌组织中c-fos蛋白表达与HPV感染的相关性

目的探讨宫颈鳞癌及宫颈病变组织中原癌基因c-fos蛋白和人乳头状瘤病毒(HPV)表达及其与宫颈鳞癌发生、发展的关系。方法选取承德医学院附属医院妇科门诊及病房经病理确诊为宫颈鳞癌和宫颈病变组织患者共148例。依据病理学诊断结果分为慢性宫颈炎症组(31例)、低级别鳞状上皮内病变(LSIL)组(35例)、高级别鳞状上皮内病变(HSIL)组(33例)及宫颈鳞状细胞癌组(49例)。分别采用PCR-反向点杂交技术及免疫组织化学PV二步法检测各组织中HPV-DNA和c-fos蛋白表达。结果宫颈鳞癌组织中HPV感染和c-fos蛋白表达高于慢性宫颈炎症组、LSIL组及HSIL组(P<0.05);宫颈鳞癌组c-fos蛋白表达与肿瘤的分化程度、TNM分期、有无淋巴结转移密切相关(P <0.05);HPV阳性宫颈鳞癌患者c-fos蛋白表达率高于HPV阴性患者(P <0.05)。结论 c-fos蛋白在宫颈鳞癌及宫颈病变组织均有一定程度表达,随宫颈病变程度的加重c-fos蛋白表达逐渐升高,宫颈鳞癌组织中c-fos表达与HPV感染密切相关。

Significance of serum glucagon-like peptide-1 and matrix Gla protein levels in patients with diabetes and osteoporosis

BACKGROUND Osteoporosis is a systemic bone disease characterized by decreased bone mass,impaired bone mass,and reduced bone strength that leads to increased bone fragility and fracture.Type 2 diabetes mellitus(T2DM)complicated with osteoporosis is a common systemic metabolic bone disease,and reduced bone mass and bone strength are considered the main clinical features;however,the pathogenesis of this disease has not been fully clarified.Its occurrence is considered related to sex,age,and genetic factors.There are many risk factors for diabetes complicated with osteoporosis.Therefore,exploring these risk factors will help prevent it.AIM To investigate the relationships among serum glucagon-like peptide-1(GLP-1)levels,matrix Gla protein(MGP)levels,and diabetes with osteoporosis.METHODS Sixty patients with T2DM complicated with osteoporosis confirmed by the endocrinology department of our hospital were selected as the case group.Sixty T2DM patients with bone loss were selected as the control group.Sixty healthy participants were selected as the healthy group.The general data,bone mineral density index,and bone metabolic markers of the three groups were compared.The relationships among GLP-1 levels,MGP levels,and the bone mineral density index of the case group were analyzed using linear correlation analysis and a logistic regression model.RESULTS Differences in sex,smoking,and drinking among the case group,control group,and healthy group were not statistically significant(P>0.05).The mean age of the case group was older than those of the control and healthy groups(P<0.05).The body mass index,fasting plasma glucose level,HbA1c level,hypertension rate,and coronary heart disease rate of the case and control groups were higher than those of the healthy group(P<0.05).The serum GLP-1 and MGP levels of the case group were lower than those of the control and healthy groups;these differences were statistically significant(P<0.05).The serum GLP-1 and MGP levels of the control group were lower than those of the healthy group;these differences were statistically significant(P<0.05).The serum GLP-1 and MGP levels of the case group were significantly positively correlated with the bone mineral density values of the hip and lumbar spine(P<0.05).The results of the logistic regression model showed that age and duration of diabetes were independent risk factors for osteoporosis in diabetic patients(P<0.05)and that increased GLP-1 and MGP values were protective factors against osteoporosis in diabetic patients(P<0.05).CONCLUSION Serum GLP-1 and MGP levels of diabetic patients with osteoporosis were significantly decreased and positively correlated with bone mineral density and were independent risk factors for osteoporosis in diabetic patients.

Different effects of sevoflurane and propofol on cognitive function and neuronal apoptosis in aged rats

Objective:To study the different effects of sevoflurane and propofol on cognitive function and neuronal apoptosis in aged rats.Methods:19-22-month-old male SD rats were selected as experimental animals and randomly divided into control group, sevoflurane group and propofol group, sevoflurane group were given 2% sevoflurane inhalation and propofol group were given intraperitoneal injection of 50 mg propofol. 7 d and 14 d after intervention, Morris water maze experiment was conducted to evaluate the cognitive function of rats;14 d after intervention, the brain tissue was isolated to determine the contents of cytokines and the expression of apoptosis genes.Results: The latent period of sevoflurane group and propofol group were longer than that of control group, and the times of original platform crossing were less than those of control group;the latent period of sevoflurane group was shorter than that of propofol group, and the times of original platform crossing were more than those of propofol group;TNF-α and IL-1β contents as well as Bax, CHOP, caspase-3, LC-3 and Beclin-1 mRNA expression in brain tissue of sevoflurane group and propofol group were higher than those of control group whereas HIF-1α, BDNF and VEGF contents were lower than those of control group, and TNF-α and IL-1β contents as well as Bax, CHOP, caspase-3, LC-3 and Beclin-1 mRNA expression in brain tissue of sevoflurane group were lower than those of propofol group whereas HIF-1α, BDNF and VEGF contents were higher than those of propofol group. Conclusion: Both sevoflurane and propofol can cause cognitive impairment and neuronal apoptosis in aged rats, but the damage of sevoflurane is weaker than that of propofol.

The Existence and Application of Strongly Idempotent Self-orthogonal Row Latin Magic Arrays

Let N = {0, 1, ···, n-1}. A strongly idempotent self-orthogonal row Latin magic array of order n（SISORLMA（n） for short） based on N is an n × n array M satisfying the following properties：（1） each row of M is a permutation of N, and at least one column is not a permutation of N;（2） the sums of the n numbers in every row and every column are the same;（3） M is orthogonal to its transpose;（4） the main diagonal and the back diagonal of M are 0, 1, ···, n-1 from left to right. In this paper, it is proved that an SISORLMA（n）exists if and only if n ？ {2, 3}. As an application, it is proved that a nonelementary rational diagonally ordered magic square exists if and only if n ？ {2, 3}, and a rational diagonally ordered magic square exists if and only if n ≠2.