Magnetic fields are significant in the structure and evolution of stars. We present a comprehensive catalog of 1784known magnetic stars, detailing their identifications, HD numbers, precise locations, spectral types a...Magnetic fields are significant in the structure and evolution of stars. We present a comprehensive catalog of 1784known magnetic stars, detailing their identifications, HD numbers, precise locations, spectral types and averaged quadratic effective magnetic fields among other important information. The group comprises 177 O-type stars, 551B-type stars, 520 A-type stars, 91 F-type stars, 53 G-type stars, 61 K-type stars, 31 M-type stars and an additional300 stars whose spectral classification remains indeterminate. Our analysis examines the statistical properties of these magnetic stars. The relative integrated distribution function and number distribution function for all magnetic stars of the same spectral type can be effectively approximated using an exponential function of the averaged quadratic effective magnetic field. The analysis further reveals that A and B-type stars possess the strongest mean magnetic fields, indicating an easier detection of their magnetic fields.展开更多
Pt/CeO_(2)-C catalysts with CeO_(2)pre-calcined at 300-600 ℃were synthesized by combining hydrothermal calcination and wet im-pregnation.The effects of the pre-calcined CeO_(2)on the performance of Pt/CeO_(2)-C catal...Pt/CeO_(2)-C catalysts with CeO_(2)pre-calcined at 300-600 ℃were synthesized by combining hydrothermal calcination and wet im-pregnation.The effects of the pre-calcined CeO_(2)on the performance of Pt/CeO_(2)-C catalysts in methanol oxidation were investigated.The Pt/CeO_(2)-C catalysts with pre-calcined CeO_(2)at 300-600 ℃showed an average particle size of 2.6-2.9 nm and exhibited better methanol elec-tro-oxidation catalytic activity than the commercial Pt/C catalyst.In specific,the Pt/CeO_(2)-C catalysts with pre-calcined CeO_(2)at 400 ℃dis-played the highest electrochemical surface area value of 68.14 m2·g−1 and If/Ib ratio(the ratio of the forward scanning peak current density(If)and the backward scanning peak current density(Ib))of 1.26,which are considerably larger than those(53.23 m2·g−1 and 0.79,respectively)of the commercial Pt/C catalyst,implying greatly enhanced CO tolerance.展开更多
High-quality superconducting Ca_(1-x)Na_(x)Fe_(2)As_(2)single crystals have been successfully grown by the NaAs-flux method,with sodium doping level x=0.4–0.64.The typical sizes of these crystals are more than 10 mm ...High-quality superconducting Ca_(1-x)Na_(x)Fe_(2)As_(2)single crystals have been successfully grown by the NaAs-flux method,with sodium doping level x=0.4–0.64.The typical sizes of these crystals are more than 10 mm in ab-plane and~0.1 mm along c-axis in thickness.X-ray diffraction,resistance and magnetization measurements are carried out to characterize the quality of these crystals.While no signature of magnetic phase transitions is detected in the normal state,bulk superconductivity is found for these samples,with a sharp transition at T_(c) ranging from 19.8 K(x=0.4)to 34.8 K(x=0.64).The doping dependences of the c-axis parameter and T_(c) are consistent with previous reports,suggesting a possible connection between the lattice parameters and superconductivity.展开更多
BACKGROUND After undergoing radical cystectomy combined with hysterectomy,female patients may suffer from pelvic organ prolapse due to the destruction of pelvic structures,which mainly manifests as the prolapse of tis...BACKGROUND After undergoing radical cystectomy combined with hysterectomy,female patients may suffer from pelvic organ prolapse due to the destruction of pelvic structures,which mainly manifests as the prolapse of tissues of the vulva to varying degrees and can be accompanied by symptoms,such as bleeding and inflammation.Once this complication is present,surgical intervention is needed to resolve it.Therefore,preventing and managing this complication is especially important.CASE SUMMARY The postoperative occurrence of acute enterocele is rare,and a case of acute small bowel vaginosis 2 mo after radical cystectomy with hysterectomy is reported.When the patient was admitted,physical examination revealed that the small bowel was displaced approximately 20 cm because of vaginocele.A team of gynecological,general surgery,and urological surgeons was employed to return the small bowel and repair the lacerated vaginal wall during the emergency operation.Eventually,the patient recovered,and no recurrence was seen in the half year of follow-up.CONCLUSION We review the surgical approach for such patients,analyze high-risk factors for the disease and suggest corresponding preventive measures.展开更多
BACKGROUND Relapsing polychondritis is a rare multisystem autoimmune disease that mainly involves systemic cartilage and proteoglycan-rich tissues.If the larynx and trachea are involved,the patient’s condition deteri...BACKGROUND Relapsing polychondritis is a rare multisystem autoimmune disease that mainly involves systemic cartilage and proteoglycan-rich tissues.If the larynx and trachea are involved,the patient’s condition deteriorates rapidly.When relapsing polychondritis becomes more advanced,the airways collapse and treatment is difficult,rendering a poor prognosis.Therefore,the diagnosis method,treatment strategy and prognosis of relapsing polychondritis with larynx and trachea involvement need to be elucidated to improve clinicians’awareness of the disease.CASE SUMMARY A man and a woman were admitted because of breathlessness.Relapsing polychondritis was diagnosed after a series of accessory examinations.They were both treated with glucocorticoids and immunosuppressants,and underwent tracheotomy as their breathing difficulties could not be relieved by the medication.CONCLUSION The two cases highlight the importance of the timely diagnosis,full evaluation and initiating individualized treatment of relapsing polychondritis with larynx and trachea involvement.Laryngoscopy,bronchoscopy and pathological examination are helpful in diagnosis of this disease.展开更多
This study provides a comprehensive overview of the challenges to achieving carbon neutrality at the county level in China and offers targeted recommendations,laying the groundwork for future specialized research in t...This study provides a comprehensive overview of the challenges to achieving carbon neutrality at the county level in China and offers targeted recommendations,laying the groundwork for future specialized research in this area.A total of 283 relevant studies(2004-2023)were analyzed to assess county-level carbon emissions through three phases:bibliometric analysis,frontier analysis,and future prospects.Bibliometric findings reveal that publication trends were largely influenced by domestic and foreign policies.Keyword cluster discerns ten primary themes,ranging from conceptual frameworks to research methodologies.The frontier analysis of the literature highlights the leading research areas,which include carbon neutrality pathway,driving factors,spatiotemporal variation of carbon emissions,the co-effects of pollutants and carbon reduction,and carbon emissions in China's rural areas.Drawing from the results of bibliometric and frontier analyses,this study elucidates the recommendations for achieving carbon neutrality at the county level from three perspectives:effective regional policy guidance,emphasis on ecological conservation,and the deployment of advanced carbon reduction and sequestration technologies.This study enriches the body of knowledge on carbon emissions at the county level and holds significant implications for China's comprehensive push towards achieving its carbon neutrality objectives.展开更多
Purpose:To identify the potential target genes of blast lung injury(BLI)for the diagnosis and treatment.Methods:This is an experimental study.The BLI models in rats and goats were established by conducting a fuel-air ...Purpose:To identify the potential target genes of blast lung injury(BLI)for the diagnosis and treatment.Methods:This is an experimental study.The BLI models in rats and goats were established by conducting a fuel-air explosive power test in an unobstructed environment,which was subsequently validated through hematoxylin-eosin staining.Transcriptome sequencing was performed on lung tissues from both goats and rats.Differentially expressed genes were identified using the criteria ofq≤0.05 and|log2 fold change|≥1.Following that,enrichment analyses were conducted for gene ontology and the Kyoto Encyclopedia of Genes and Genomes pathways.The potential target genes were further confirmed through quantitative real-time polymerase chain reaction and enzyme linked immunosorbent assay.Results:Observations through microscopy unveiled the presence of reddish edema fluid,erythrocytes,and instances of focal or patchy bleeding within the alveolar cavity.Transcriptome sequencing analysis identified a total of 83 differentially expressed genes in both rats and goats.Notably,49 genes exhibited a consistent expression pattern,with 38 genes displaying up-regulation and 11 genes demonstrating down-regulation.Enrichment analysis highlighted the potential involvement of the interleukin-17 signaling pathway and vascular smooth muscle contraction pathway in the underlying mechanism of BLI.Furthermore,the experimental findings in both goats and rats demonstrated a strong association between BLI and several key genes,including anterior gradient 2,ankyrin repeat domain 65,bactericidal/permeability-increasing fold containing family A member 1,bactericidal/permeability-increasing fold containing family B member 1,and keratin 4,which exhibited up-regulation.Conclusions:Anterior gradient 2,ankyrin repeat domain 65,bactericidal/permeability-increasing fold containing family A member 1,bactericidal/permeability-increasing fold containing family B member 1,and keratin 4 hold potential as target genes for the prognosis,diagnosis,and treatment of BLI.展开更多
Background: Fine particulate matter (PM2.5) exacerbates airway inflammation and hyperreactivity in patients with asthma, but the mechanism remains unclear. The aim of this study was to observe the effects of prolon...Background: Fine particulate matter (PM2.5) exacerbates airway inflammation and hyperreactivity in patients with asthma, but the mechanism remains unclear. The aim of this study was to observe the effects of prolonged exposure to high concentrations of PM2.5 on the pathology and airway hyperresponsiveness (AHR) of BALB/c mice undergoing sensitization and challenge with ovalbumin (OVA) and to observe the effects of apoptosis and T-cell immunoglobulin and mucin domain 1 (TIM-1) in this process. Methods: Forty female BALB/c mice were divided into four groups: control group, OVA group, OVA/PM group, and PM group (n - 10 in each group). Mice in the control group were exposed to filtered clean air. Mice in the OVA group were sensitized and challenged with OVA. Mice in the OVA/PM group were sensitized and challenged as in the OVA group and then exposed to PM2.5 for 4 h per day and 5 days per week for a total of 8 weeks using a nose-only "PM2.5 online enrichment system" in The Second Hospital of Hebei Medical University. Mice in the PM group were exposed to the PM2.5 online enrichment system only. AHR was detected. Bronchoalveolar lavage fluid (BALF) was collected for cell classification. The levels of interleukin-4 (IL-4), IL-5, and IL-33 in BALF were measured using enzyme-linked immunosorbent assay. Changes in histological structures were examined by light microscopy, and changes in ultramicrostructures were detected by electron microscopy. Apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay in the lung tissues. Western blotting and immunohistochemistry were utilized to analyze the expression of Bcl-2, Bax, and TIM-1 in the lungs. Results: The results showed that AHR in the OVA/PM group was significantly more severe than that in the OVA and PM groups (P 〈 0.05). AHR in the PM group was also considerably more severe than that in the control group (P 〈 0.05). The BALF of OVA/PM group (28.00± 6.08 vs. 12.33 ±4.51, t = 4.631, P = 0.002) and PM group (29.00 ± 3.00 vs. 12.33 ± 4.51, t = 4.927, P = 0.001) had more lymphocytes than the BALF of the control group. The number of neutrophils in the BALF of the OVA/PM group (6.67 ± 1.53 vs. 3.33 ± 1.53, t = 2.886, P = 0.020) and PM group (6.67 ± 1.53 vs. 3.33 ± 1.53, t = 2.886, P = 0.020) was much higher than those in the BALF of OVA group (P 〈 0.05). TUNEL assays showed that the number of apoptotic cells in the OVA/PM group was significantly higher than that in the OVA group (Tunel immunohistochemical scores [IHS%], 1.20 ± 0.18 vs. 0.51 ± 0.03, t = 8.094, P 〈 0.001) and PM group (Tunel IHS%, 1.20 ± 0.18 vs. 0.51 ±0.09, t = 8.094, P〈 0.001), and that the number of apoptotic cells in the PM group was significantly higher than that in the control group (Tunel IHS%, 0.51 ± 0.09 vs. 0.26 ± 0.03, t = 2.894, P = 0.020). The concentrations of IL-4 (77.44 ± 11.19 vs. 48.02 ±10.02 pg/ml, t = 4.595, P= 0.002) and IL-5 (15.65 ± 1.19vs. 12.35±0.95pg/ml, t=3.806,P=0.005) and the Bax/Bcl-2 ratio (1.51 ± 0.18 vs. 0.48 ± 0.10, t = 9.654, P 〈 0.001) and TIM-1/β-actin ratio (0.78 ± 0.11 vs. 0.40 ±0.06, t = 6.818, P 〈 0.001) in the OVA/PM group were increased compared to those in the OVA group. The concentrations of IL-4 (77.44 ± 11.19 vs. 41.47 ± 3.40 pg/ml, t = 5.617, P= 0.001) and IL-5 ( 15.65±1. 19 vs. 10.99 ± 1.40 pg/ml, t = 5.374, P = 0.001 ) and the Bax/Bcl-2 ratio (1.51 ±0.18 vs. 0.97 ± 0.16, t = 5.000, P = 0.001) and TIM-1/β-actin ratio (0.78 ± 0.11 vs. 0.31 ± 0.06,t = 8.545, P 〈 0.001 ) in the OVA/PM group were increased compared to those in the PM group. The concentration oflL-4 (41.47 ±3.40 vs. 25.46 ± 2.98 pg/ml, t = 2.501, P = 0.037) and the Bax/Bcl-2 ratio (0.97 ± 0.16 vs. 0.18 ± 0.03, t = 7.439, P 〈 0.001) and TIM-1/β-actin ratio (0.31 ± 0.06 vs. 0.02 ± 0.01, t = 5.109, P = 0.001) in the PM group were also higher than those in the control group. Conclusions: Exacerbated AHR associated with allergic asthma caused by PMz5 is related to increased apoptosis and TIM-1 activation. These data might provide insights into therapeutic targets for the treatment of acute exacerbations of asthma induced by PM2.5.展开更多
Green light-emitting polyfluorenes containing 3,7-bis(4-hexylthiophen-2-yl)dibenzo[b,d]thiophene 5,5-dioxide(DHTSO)unit were synthesized.All the resulted polymers show high thermal stability with the decomposition...Green light-emitting polyfluorenes containing 3,7-bis(4-hexylthiophen-2-yl)dibenzo[b,d]thiophene 5,5-dioxide(DHTSO)unit were synthesized.All the resulted polymers show high thermal stability with the decomposition temperatures(T_d)over 420°C and the glass transition temperatures(T_g)over 75°C.The polymers exhibit the enhanced highest occupied molecular orbital(HOMO)energy levels and the depressed lowest unoccupied molecular orbital(LUMO)energy levels with the increase of DHTSO unit in polymers.The photoluminescence(PL)spectra of the polymers show positive solvatochromism in solution with the variation of solution polarities,indicating remarkable intramolecular charge transfer(ICT)effect in the polymers containing DHTSO moiety.The fluorescence quantum yields((37)_(PL))are in the range of 34%-67%for PF-DHTSOs in film.All polymers possess two photon absorption(TPA)properties,and the TPA cross sections(?_2)are enhanced with increasing DHTSO unit in polymers.The highest?_2 is 2392 GM for PF-DHTSO15 in chloroform solution upon 740 nm excitation.The device of PF-DHTSO15 shows green emission with the Commission Internationale de L’.Eclairage(CIE)coordinates of(0.26,0.59),and the maximum luminous efficiency(LE_(max))of 10.8 cd·A^(-1) with the configuration of ITO/PEDOT:PSS/EL/Cs F/Al.These results indicate that introducing DHTSO unit into polyfluorene backbone could be a promising molecular design strategy for TPA and effective green-light emission.展开更多
基金the generous support of the National Natural Science Foundation of China(NSFC,Grant Nos.U2031204,12163005 and 12288102)the Natural Science Foundation of Xinjiang Nos.2021D01C075,2022D01D85,2022TSYCLJ0006 and 2022D01E86+1 种基金the science research grants from the China Manned Space Project with Nos.CMSCSST-2021-A10 and CMS-CSST-2021-A08the Chinese Academy of Sciences(CAS)“Light of West China”Program,No.2022-XBQNXZ-013。
文摘Magnetic fields are significant in the structure and evolution of stars. We present a comprehensive catalog of 1784known magnetic stars, detailing their identifications, HD numbers, precise locations, spectral types and averaged quadratic effective magnetic fields among other important information. The group comprises 177 O-type stars, 551B-type stars, 520 A-type stars, 91 F-type stars, 53 G-type stars, 61 K-type stars, 31 M-type stars and an additional300 stars whose spectral classification remains indeterminate. Our analysis examines the statistical properties of these magnetic stars. The relative integrated distribution function and number distribution function for all magnetic stars of the same spectral type can be effectively approximated using an exponential function of the averaged quadratic effective magnetic field. The analysis further reveals that A and B-type stars possess the strongest mean magnetic fields, indicating an easier detection of their magnetic fields.
基金This work was financially supported by the National Nat-ural Science Foundation of China(No.51774145).
文摘Pt/CeO_(2)-C catalysts with CeO_(2)pre-calcined at 300-600 ℃were synthesized by combining hydrothermal calcination and wet im-pregnation.The effects of the pre-calcined CeO_(2)on the performance of Pt/CeO_(2)-C catalysts in methanol oxidation were investigated.The Pt/CeO_(2)-C catalysts with pre-calcined CeO_(2)at 300-600 ℃showed an average particle size of 2.6-2.9 nm and exhibited better methanol elec-tro-oxidation catalytic activity than the commercial Pt/C catalyst.In specific,the Pt/CeO_(2)-C catalysts with pre-calcined CeO_(2)at 400 ℃dis-played the highest electrochemical surface area value of 68.14 m2·g−1 and If/Ib ratio(the ratio of the forward scanning peak current density(If)and the backward scanning peak current density(Ib))of 1.26,which are considerably larger than those(53.23 m2·g−1 and 0.79,respectively)of the commercial Pt/C catalyst,implying greatly enhanced CO tolerance.
基金Project supported by the National Key Research and Development Program of China(Grant No.2018YFA0704200)the National Natural Science Foundation of China(Grant Nos.11822411 and 11961160699)+4 种基金the Strategic Priority Research Program(B)of the CAS(Grants Nos.XDB25000000 and XDB33000000)the K.C.Wong Education Foundation(Grant No.GJTD-2020-01)the Youth Innovation Promotion Association of CAS(Grant No.Y202001)the Postdoctoral Innovative Talent program(Grant No.BX2021018)the China Postdoctoral Science Foundation(Grant No.2021M700250)。
文摘High-quality superconducting Ca_(1-x)Na_(x)Fe_(2)As_(2)single crystals have been successfully grown by the NaAs-flux method,with sodium doping level x=0.4–0.64.The typical sizes of these crystals are more than 10 mm in ab-plane and~0.1 mm along c-axis in thickness.X-ray diffraction,resistance and magnetization measurements are carried out to characterize the quality of these crystals.While no signature of magnetic phase transitions is detected in the normal state,bulk superconductivity is found for these samples,with a sharp transition at T_(c) ranging from 19.8 K(x=0.4)to 34.8 K(x=0.64).The doping dependences of the c-axis parameter and T_(c) are consistent with previous reports,suggesting a possible connection between the lattice parameters and superconductivity.
文摘BACKGROUND After undergoing radical cystectomy combined with hysterectomy,female patients may suffer from pelvic organ prolapse due to the destruction of pelvic structures,which mainly manifests as the prolapse of tissues of the vulva to varying degrees and can be accompanied by symptoms,such as bleeding and inflammation.Once this complication is present,surgical intervention is needed to resolve it.Therefore,preventing and managing this complication is especially important.CASE SUMMARY The postoperative occurrence of acute enterocele is rare,and a case of acute small bowel vaginosis 2 mo after radical cystectomy with hysterectomy is reported.When the patient was admitted,physical examination revealed that the small bowel was displaced approximately 20 cm because of vaginocele.A team of gynecological,general surgery,and urological surgeons was employed to return the small bowel and repair the lacerated vaginal wall during the emergency operation.Eventually,the patient recovered,and no recurrence was seen in the half year of follow-up.CONCLUSION We review the surgical approach for such patients,analyze high-risk factors for the disease and suggest corresponding preventive measures.
文摘BACKGROUND Relapsing polychondritis is a rare multisystem autoimmune disease that mainly involves systemic cartilage and proteoglycan-rich tissues.If the larynx and trachea are involved,the patient’s condition deteriorates rapidly.When relapsing polychondritis becomes more advanced,the airways collapse and treatment is difficult,rendering a poor prognosis.Therefore,the diagnosis method,treatment strategy and prognosis of relapsing polychondritis with larynx and trachea involvement need to be elucidated to improve clinicians’awareness of the disease.CASE SUMMARY A man and a woman were admitted because of breathlessness.Relapsing polychondritis was diagnosed after a series of accessory examinations.They were both treated with glucocorticoids and immunosuppressants,and underwent tracheotomy as their breathing difficulties could not be relieved by the medication.CONCLUSION The two cases highlight the importance of the timely diagnosis,full evaluation and initiating individualized treatment of relapsing polychondritis with larynx and trachea involvement.Laryngoscopy,bronchoscopy and pathological examination are helpful in diagnosis of this disease.
基金This work was financially supported by the Chinese National Natural Science Foundation(42071293,42371308)the Science and Technology Innovation Plan of Shanghai Science and Technology Commission(22DZ1209500)the Fundamental Research Funds for the Central Universities.
文摘This study provides a comprehensive overview of the challenges to achieving carbon neutrality at the county level in China and offers targeted recommendations,laying the groundwork for future specialized research in this area.A total of 283 relevant studies(2004-2023)were analyzed to assess county-level carbon emissions through three phases:bibliometric analysis,frontier analysis,and future prospects.Bibliometric findings reveal that publication trends were largely influenced by domestic and foreign policies.Keyword cluster discerns ten primary themes,ranging from conceptual frameworks to research methodologies.The frontier analysis of the literature highlights the leading research areas,which include carbon neutrality pathway,driving factors,spatiotemporal variation of carbon emissions,the co-effects of pollutants and carbon reduction,and carbon emissions in China's rural areas.Drawing from the results of bibliometric and frontier analyses,this study elucidates the recommendations for achieving carbon neutrality at the county level from three perspectives:effective regional policy guidance,emphasis on ecological conservation,and the deployment of advanced carbon reduction and sequestration technologies.This study enriches the body of knowledge on carbon emissions at the county level and holds significant implications for China's comprehensive push towards achieving its carbon neutrality objectives.
基金Natural Science Basis Research Plan in Shaanxi Province of China(Program No.2023-JC-YB-684).
文摘Purpose:To identify the potential target genes of blast lung injury(BLI)for the diagnosis and treatment.Methods:This is an experimental study.The BLI models in rats and goats were established by conducting a fuel-air explosive power test in an unobstructed environment,which was subsequently validated through hematoxylin-eosin staining.Transcriptome sequencing was performed on lung tissues from both goats and rats.Differentially expressed genes were identified using the criteria ofq≤0.05 and|log2 fold change|≥1.Following that,enrichment analyses were conducted for gene ontology and the Kyoto Encyclopedia of Genes and Genomes pathways.The potential target genes were further confirmed through quantitative real-time polymerase chain reaction and enzyme linked immunosorbent assay.Results:Observations through microscopy unveiled the presence of reddish edema fluid,erythrocytes,and instances of focal or patchy bleeding within the alveolar cavity.Transcriptome sequencing analysis identified a total of 83 differentially expressed genes in both rats and goats.Notably,49 genes exhibited a consistent expression pattern,with 38 genes displaying up-regulation and 11 genes demonstrating down-regulation.Enrichment analysis highlighted the potential involvement of the interleukin-17 signaling pathway and vascular smooth muscle contraction pathway in the underlying mechanism of BLI.Furthermore,the experimental findings in both goats and rats demonstrated a strong association between BLI and several key genes,including anterior gradient 2,ankyrin repeat domain 65,bactericidal/permeability-increasing fold containing family A member 1,bactericidal/permeability-increasing fold containing family B member 1,and keratin 4,which exhibited up-regulation.Conclusions:Anterior gradient 2,ankyrin repeat domain 65,bactericidal/permeability-increasing fold containing family A member 1,bactericidal/permeability-increasing fold containing family B member 1,and keratin 4 hold potential as target genes for the prognosis,diagnosis,and treatment of BLI.
基金This study was supported by a grant from the National Natural Science Foundation of China (No. 81770020).
文摘Background: Fine particulate matter (PM2.5) exacerbates airway inflammation and hyperreactivity in patients with asthma, but the mechanism remains unclear. The aim of this study was to observe the effects of prolonged exposure to high concentrations of PM2.5 on the pathology and airway hyperresponsiveness (AHR) of BALB/c mice undergoing sensitization and challenge with ovalbumin (OVA) and to observe the effects of apoptosis and T-cell immunoglobulin and mucin domain 1 (TIM-1) in this process. Methods: Forty female BALB/c mice were divided into four groups: control group, OVA group, OVA/PM group, and PM group (n - 10 in each group). Mice in the control group were exposed to filtered clean air. Mice in the OVA group were sensitized and challenged with OVA. Mice in the OVA/PM group were sensitized and challenged as in the OVA group and then exposed to PM2.5 for 4 h per day and 5 days per week for a total of 8 weeks using a nose-only "PM2.5 online enrichment system" in The Second Hospital of Hebei Medical University. Mice in the PM group were exposed to the PM2.5 online enrichment system only. AHR was detected. Bronchoalveolar lavage fluid (BALF) was collected for cell classification. The levels of interleukin-4 (IL-4), IL-5, and IL-33 in BALF were measured using enzyme-linked immunosorbent assay. Changes in histological structures were examined by light microscopy, and changes in ultramicrostructures were detected by electron microscopy. Apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay in the lung tissues. Western blotting and immunohistochemistry were utilized to analyze the expression of Bcl-2, Bax, and TIM-1 in the lungs. Results: The results showed that AHR in the OVA/PM group was significantly more severe than that in the OVA and PM groups (P 〈 0.05). AHR in the PM group was also considerably more severe than that in the control group (P 〈 0.05). The BALF of OVA/PM group (28.00± 6.08 vs. 12.33 ±4.51, t = 4.631, P = 0.002) and PM group (29.00 ± 3.00 vs. 12.33 ± 4.51, t = 4.927, P = 0.001) had more lymphocytes than the BALF of the control group. The number of neutrophils in the BALF of the OVA/PM group (6.67 ± 1.53 vs. 3.33 ± 1.53, t = 2.886, P = 0.020) and PM group (6.67 ± 1.53 vs. 3.33 ± 1.53, t = 2.886, P = 0.020) was much higher than those in the BALF of OVA group (P 〈 0.05). TUNEL assays showed that the number of apoptotic cells in the OVA/PM group was significantly higher than that in the OVA group (Tunel immunohistochemical scores [IHS%], 1.20 ± 0.18 vs. 0.51 ± 0.03, t = 8.094, P 〈 0.001) and PM group (Tunel IHS%, 1.20 ± 0.18 vs. 0.51 ±0.09, t = 8.094, P〈 0.001), and that the number of apoptotic cells in the PM group was significantly higher than that in the control group (Tunel IHS%, 0.51 ± 0.09 vs. 0.26 ± 0.03, t = 2.894, P = 0.020). The concentrations of IL-4 (77.44 ± 11.19 vs. 48.02 ±10.02 pg/ml, t = 4.595, P= 0.002) and IL-5 (15.65 ± 1.19vs. 12.35±0.95pg/ml, t=3.806,P=0.005) and the Bax/Bcl-2 ratio (1.51 ± 0.18 vs. 0.48 ± 0.10, t = 9.654, P 〈 0.001) and TIM-1/β-actin ratio (0.78 ± 0.11 vs. 0.40 ±0.06, t = 6.818, P 〈 0.001) in the OVA/PM group were increased compared to those in the OVA group. The concentrations of IL-4 (77.44 ± 11.19 vs. 41.47 ± 3.40 pg/ml, t = 5.617, P= 0.001) and IL-5 ( 15.65±1. 19 vs. 10.99 ± 1.40 pg/ml, t = 5.374, P = 0.001 ) and the Bax/Bcl-2 ratio (1.51 ±0.18 vs. 0.97 ± 0.16, t = 5.000, P = 0.001) and TIM-1/β-actin ratio (0.78 ± 0.11 vs. 0.31 ± 0.06,t = 8.545, P 〈 0.001 ) in the OVA/PM group were increased compared to those in the PM group. The concentration oflL-4 (41.47 ±3.40 vs. 25.46 ± 2.98 pg/ml, t = 2.501, P = 0.037) and the Bax/Bcl-2 ratio (0.97 ± 0.16 vs. 0.18 ± 0.03, t = 7.439, P 〈 0.001) and TIM-1/β-actin ratio (0.31 ± 0.06 vs. 0.02 ± 0.01, t = 5.109, P = 0.001) in the PM group were also higher than those in the control group. Conclusions: Exacerbated AHR associated with allergic asthma caused by PMz5 is related to increased apoptosis and TIM-1 activation. These data might provide insights into therapeutic targets for the treatment of acute exacerbations of asthma induced by PM2.5.
文摘Green light-emitting polyfluorenes containing 3,7-bis(4-hexylthiophen-2-yl)dibenzo[b,d]thiophene 5,5-dioxide(DHTSO)unit were synthesized.All the resulted polymers show high thermal stability with the decomposition temperatures(T_d)over 420°C and the glass transition temperatures(T_g)over 75°C.The polymers exhibit the enhanced highest occupied molecular orbital(HOMO)energy levels and the depressed lowest unoccupied molecular orbital(LUMO)energy levels with the increase of DHTSO unit in polymers.The photoluminescence(PL)spectra of the polymers show positive solvatochromism in solution with the variation of solution polarities,indicating remarkable intramolecular charge transfer(ICT)effect in the polymers containing DHTSO moiety.The fluorescence quantum yields((37)_(PL))are in the range of 34%-67%for PF-DHTSOs in film.All polymers possess two photon absorption(TPA)properties,and the TPA cross sections(?_2)are enhanced with increasing DHTSO unit in polymers.The highest?_2 is 2392 GM for PF-DHTSO15 in chloroform solution upon 740 nm excitation.The device of PF-DHTSO15 shows green emission with the Commission Internationale de L’.Eclairage(CIE)coordinates of(0.26,0.59),and the maximum luminous efficiency(LE_(max))of 10.8 cd·A^(-1) with the configuration of ITO/PEDOT:PSS/EL/Cs F/Al.These results indicate that introducing DHTSO unit into polyfluorene backbone could be a promising molecular design strategy for TPA and effective green-light emission.