Nerve autografts and tissue-engineered materials for the repair of peripheral nerve injuries: a 5-year bibliometric analysis

With advances in biomedical methods, tissue-engineered materials have developed rapidly as an alternative to nerve autografts for the repair of peripheral nerve injuries. However, the materials selected for use in the repair of peripheral nerve injuries, in particular multiple injuries and largegap defects, must be chosen carefully. Various methods and materials for protecting the healthy tissue and repairing peripheral nerve injuries have been described, and each method or material has advantages and disadvantages. Recently, a large amount of research has been focused on tissue-engineered materials for the repair of peripheral nerve injuries. Using the keywords ＂peripheral nerve injury＂, ＂autotransplant＂, ＂nerve graft＂, and ＂biomaterial＂, we retrieved publications using tissue-engineered materials for the repair of peripheral nerve injuries appearing in the Web of Science from 2010 to 2014. The country with the most total publications was the USA. The institutions that were the most productive in this field include Hannover Medical School （Germany）, Washington University （USA）, and Nantong University （China）. The total number of publications using tissue-engineered materials for the repair of peripheral nerve injuries grad- ually increased over time, as did the number of Chinese publications, suggesting that China has made many scientific contributions to this field of research.

Application of Paclitaxel as Adjuvant Treatment for Benign Cicatricial Airway Stenosis

Benign cicatricial airway stenosis（BCAS） is a potentially life-threatening disease.Recurrence occurs frequently after endoscopic treatment.Paclitaxel is known to prevent restenosis,but its clinical efficacy and safety is undetermined.Therefore,in this study,we investigated the efficacy and associated complications of paclitaxel as adjuvant treatment for BCAS of different etiologies.The study cohort included 28 patients with BCAS resulting from tuberculosis,intubation,tracheotomy,and other etiologies.All patients were treated at the Department of Respiratory Diseases,Beijing Tian Tan Hospital,Capital Medical University,China,between January 2010 and August 2014.After primary treatment by balloon dilation,cryotherapy,and/or high-frequency needle-knife treatment,paclitaxel was applied to the airway mucosa at the site of stenosis using a newly developed local instillation catheter.The primary outcome measures were the therapeutic efficacy of paclitaxel as adjuvant treatment,and the incidence of complications was observed as well.According to our criteria for evaluating the clinical effects on BCAS,24 of the 28 cases achieved durable remission,three cases had remission,and one case showed no remission.Thus,the durable remission rate was 85.7%,and the combined effective rate was 96.4%.No differences in outcomes were observed among the different BCAS etiologies（P=0.144）,and few complications were observed.Our results indicated that paclitaxel as an adjuvant treatment has greater efficacy than previously reported BCAS treatment methods.

Effect of acupoint application combined with microwave treatment on the intestine barrier functional disturbance of moderately severe acute pancreatitis

Objective: To explore and analyze the effect of acupoint application combined with microwave treatment on the intestinal barrier dysfunction with moderately severe acute pancreatitis. Methods: A convenient sample of 90 moderately severe acute pancreatitis was selected from March 2017 to December 2017 in the comprehensive hospital with third grade in Tianjin. The patients were divided into group A (acupoint application combined with microwave treatment), group B (acupoint application) and group C (routine nursing). Thirty patients were included in each group. This study need to get the informed consent of the patients. Acupoint application combined with microwave treatment was used, basing on routine nursing measures in group A. Acupoint application was used by the same way and the same traditional Chinese medicine ,basing on routine nursing measures in group B. Routine nursing used in group C. C-reactive protein and the score of intestinal function were measured on 3 th day, 7 th day and 10 th day, after intervention. To record the effective ratio of the treatment after 10 days of intervention. Results: There are significant statistical difference among the three group after intervention (P < 0.05). Conclusion: In some way, acupoint application combined with microwave treatment are able to decrease the time about the recovery of intestinal barrier dysfunction in moderately severe acute pancreatitis and to alleviate the suffering of patients.

Moxibustion treatment for knee osteoarthritis： cumulative meta-analysis and trial sequential analysis

Objective： To evaluate the effect of moxibustion on knee osteoarthritis patients with cumulative meta-analysis, and trial sequential analysis was applied to test the authenticity of results. Methods： We searched PubMed, EMbase, The Cochrane Library, CNKI and CBM to collect RCTs about moxibustion on knee osteoarthritis patients. The selection of literature, data extraction and evaluation of eligible literature were carried out independently by two reviewers. Then Stata11.0 software was used for data analysis. Result： Totally 11 RCTs involved 1005 patients were recruited. The results of meta-analysis showed that no significant differences were found between the two groups in VAS, moxibustion is better than drug therapy in effective rate [OR = 0.40, 95%CI （0.27, 0.60）] and knee score [SMD = -0.70, 95%CI （-1.22, -0.19）]. The result of trial sequential analysis indicated that the sample size didn＇t reach the TSA Boundary on VAS, the sample size of effectiveness achieved Require Information Size. In the indicator of knee score has obtained certain re- sult before reaching the Require Information Size. Conclusion： moxibustion is superior to drug therapy on treatment knee osteoarthritis. However, large sample size and high-quality studies are still needed.

Research progress of diabetes treatment guided by Traditional Chinese Medicine constitutional types

Diabetes and its complications seriously affect patients’life quality,and have a major impact on individuals,families,health systems,and national economies.Traditional Chinese Medicine constitution refers to the inherent characteristics of a relatively comprehensive and stable form,structure,physiological function,and psychological state formed under the combined effects of innate endowments and acquired in the course of an individual’s life.Many studies have shown that the constitution type is closely related to the incidence of diabetes,especially in the process of disease prevention and treatment.This article reviews the correlation of diabetes and its chronic complications with constitution types to provide theoretical basis for the treatment of diabetes based on syndrome differentiation.

Effect of moxibustion combined with acupoint application on enteral nutrition tolerance in patients with severe acute pancreatitis

Objective: To explore and analyze the effect of moxibustion combined with acupoint application on enteral nutrition tolerance, nutritional status and comfort in patients with severe acute pancreatitis. Methods: Ninety-six patients who were hospitalized in a third-grade class-A general hospital in Tianjin were selected and divided into three groups: A, B, and C, each with 32 cases. Group A uses usual treatment and care methods, group B implements acupoint application treatment, group C was treated with moxibustion on the basis of acupoint application. Intervention time is from the first day of the enteral nutrition to the 7th day, the tolerance of enteral nutrition in patients 7 days during the intervention and the level of serum albumin and pre-protein in the nutritional indicators of patients on day 3 of intervention and intervention on day 7, as well as the comfort level of the patients on the 7th day of intervention were observed and recorded. Results:(1) The difference in the incidence of enteral nutrition intolerance among the three groups was significantly statistical (P < 0.05). The incidence of enteral nutrition intolerance in group C was lower than that in group B (P < 0.05) and group A (P < 0.05). There was no significant difference between group B and group C (P > 0.05).(2) Nutritional index levels of three groups of patients, on the third day of intervention, the serum albumin and serum pre-protein were significantly statistical (P < 0.05). Comparing the two groups, the serum albumin level in group A was significantly different from that in group B (P < 0.05). There was significant difference between group A and group C (P < 0.05). There was no significant difference between the group and the group C (P > 0.05). On the 7th day of intervention, the difference between group A and group B was statistically significant (P < 0.05). There was a statistically significant difference between group A and group C (P < 0.05), group B and group C (P < 0.05). Pre-protein levels in three groups, there was a significantly statistical difference between intervention days 3 and 7 (P < 0.05), further comparison was made between the two groups. On the third day of intervention, there were significant differences between group A and group B, group A and group C, group B and group C (P < 0.05). On the 7th day of intervention, there were significant differences between group A and group B, group A and group C, group B and group C (P < 0.05).(3) On the 7th day of intervention, the comfort level of the three groups was significantly different (P < 0.05). Further comparison between the two groups was made. The comfort score of group C was higher than that of group B and group A (P < 0.05), and the score of group B was higher than group A (P < 0.05). Conclusion: Moxibustion combined with acupoint application can effectively improve the tolerance level of enteral nutrition and improve the nutritional status of patients and increase patient comfort.

The clinical effect observation of gastrointestinal function after colorectal cancer surgery with integrated of Chinese and western medicine nursing methods

Objective: To observe the clinical effect of warm moxibustion therapy and acupoint application therapy on recovery of gastrointestinal functions in postoperative patients with colorectal cancer. Methods: Eighty patients with colorectal cancer were admitted to Department of Gastrointestinal surgery Tianjin Nankai Hospital. A retrospective analysis of 20 cases as control group. 60 patients near admitted and the use of randomly divided into three treatment groups, 20 cases of each group. The control group received conventional therapy and nursing, and three treatment groups respectively received warm moxibustion therapy, acupoint application therapy and both the two methods above together additionally. Then compared the intervention effect of them. Results: General information on four groups of comparison, there was no statistics significance (P > 0.05), between the four groups were comparable. Analysis of variance of repeated measures indicated that the interaction effect of intervention time and grouping had significant influence on Chinese medicine symptom. Two groups of the Chinese medicine symptom integral changing are inconsistent (P < 0.01). Chinese medicine symptom efficacy total effective rate of treatment group was superior to the control group (P < 0.01). And first postoperative exhaust time, defecating time and eating time were earlier than control group (P < 0.01). Conclusion: Warm moxibustion therapy and acupoint application therapy had certain clinical effect on the recovery of gastrointestinal function in patients with colorectal cancer after operation, and worthy of further application in clinical practice.

Study on the timing of intervention of gastrointestinal function recovery in patients after laparoscopic cholecystectomy with acupoint application of traditional Chinese medicine

Objective: To explore the optimal timing of drug application for the recovery of gastrointestinal function in patients after laparoscopic cholecystectomy. Methods: Ninety patients undergoing laparoscopic cholecystectomy were randomly divided into three groups: control group, intervention group 1 and intervention group 2. All three groups received routine perioperative nursing. Intervention group 1 and group 2 were given acupoint application of Chinese medicine at 0.5 h and 6 h after operation, and the waveform was monitored by electrogastrogram on the 1st day before operation and 3 d after operation. The first anal exhaust time, defecation time, abdominal distension and electrogastrogram changes were analyzed and compared in the three groups. Results: Compared with the first exhaust time and defecation time after operation, the intervention group 1 was earlier than the intervention group 2, and the intervention group 2 was earlier than the control group, the difference was statistically significant (P < 0.05). The average frequency, waveform response area and average amplitude of the intervention group 1 were better than those of the intervention group 2, and the intervention group 2 was better than the control group (P < 0.05). Conclusion: Administration of Chinese medicine acupoint application within 0.5 hours after operation can promote the recovery of gastrointestinal function and improve gastric motility in patients undergoing abdominal surgery, and the curative effect is significant and safe.

The Application Progress of Auricular Therapy in Diabetes

Diabetes,as a chronic incurable disease,not only causes acute complications such as hyperglycemic hyperosmolar status,ketoacidosis and hypoglycemia,but also affects arteries and nervous system,causing the eyes,kidneys,feet,blood vessels,nerves and other organs or tissues damage,and leading to the occurrence and development of chronic diseases.The World Health Organization believes that diabetes may be the most important non-communicable global disease caused by unhealthy modern lifestyles and regards it as a major global health problem.At present,there is still a lack of specific drugs for the treatment of diabetes,and related treatment drugs are often accompanied by adverse reactions.Auricular therapy as a non-drug,non-invasive treatment method show unique advantages in the treatment of diabetes.This article reviews the mechanism of auricular therapy and its application status in diabetes,in order to broaden the mind for the auricular therapy to treat diabetes.

Research Progress of Moxibustion in Improving Postoperative Discomfort of Breast Cancer Patients

Moxibustion is one of the characteristics of Traditional Chinese medicine therapy.It has the effects of activating blood circulation and removing stasis,warming the meridians and strengthening and exorcising evil spirits.It shows unique advantages in improving postoperative symptoms of breast cancer patients.In this paper,the overview of moxibustion method,moxibustion method in improving postoperative upper limb dysfunction,cancer-related fatigue,nausea and vomiting,anxiety and depression of breast cancer patients were reviewed,and the existing deficiencies were summarized and analyzed,in order to provide reference for the clinical application of moxibustion method in improving postoperative discomfort symptoms of breast cancer patients and related research.

Characteristics of the crack tip field in high-speed railway tunnel linings under train-induced aerodynamic shockwaves

High-speed railway tunnels in various countries have continuously reported accidents of vault falling concrete blocks.Once the concrete block falling occurs,serious consequences follow,and traffic safety may be endangered.The aerodynamic shockwave evolves from the initial compression wave may be an important inducement causing the tunnel lining cracks to grow and form falling concrete blocks.A joint calculation framework is established based on ANSYS Fluent,ABAQUS,and FRANC3D for calculating the crack tip field under the aerodynamic shockwave.The intensification effect of aerodynamic shockwaves in the crack is revealed,and the evolution characteristics of the crack tip field and the influence factors of stress intensity factor(SIF)are analyzed.Results show that(1)the aerodynamic shockwave intensifies after entering the crack,resulting in more significant pressure in the crack than the input pressure.The maximum pressure of the inclined and longitudinal cracks is higher than the corresponding values of the circumferential crack,respectively.(2)The maximum SIF of the circumferential,inclined,and longitudinal crack appears at 0.5,0.68,and 0.78 times the crack front length.The maximum SIF of the circumferential crack is higher than that of the inclined and longitudinal crack.The possibility of crack growth of the circumferential crack is the highest under aerodynamic shockwaves.(3)The influence of train speed on the SIF of the circumferential crack is more than 40%.When the train speed,crack depth,and crack length change,the change of pressure in the crack is the direct cause of the change of SIF.

Fluorinated metal-organic framework for methane purification from a ternary CH_(4)/C_(2)H_(6)/C_(3)H_(8)mixture

Herein we present a fluorinated metal-organic framework of{(Me_(2)NH_(2))[Ni_(3)(μ_(3)-OH)(CF_(3)-BPDC-CF_(3))_(3)(tpt)]}_(n)(1)constructed from 2,2'-bis(trifluoromethyl)biphenyl-4,4'-dicarboxylic(CF_(3)-BPDC-CF_(3)^(2-))and 2,4,6-tri(4-pyridyl)-1,3,5-triazine(tpt)ligands,which is developed for separating propane(C_(3)H_(8))and ethane(C_(2)H_(6))from natural gas.Compound 1 preferentially adsorbs C_(3)H_(8)and C_(2)H_(6)over CH_(4)demonstrated by gas adsorption experiments.The presence of trifluoromethyl groups on the biphenyl-4,4'-dicarboxylic ligands facilitates the highly polarized micropore environments for compound 1,thus providing suitable micorpores for capturing the C_(3)H_(8)and C_(2)H_(6)molecules with larger polarizabilities and sizes compared to CH_(4)molecule.The dynamic mixture breakthrough experiments showed that compound 1 can separate C_(3)H_(8)and C_(2)H_(6)from the ternary CH_(4)/C_(2)H_(6)/C_(3)H_(8)mixtures efficiently,endowing compound 1 with excellent methane purification ability.

Scarring Airway Stenosis in Chinese Adults： Characteristics and Interventional Bronchoscopy Treatment

Background：Scarring airway stenosis is commonly seen in China as compared to other developed countries,due to the high prevalence of tuberculosis.Nowadays,interventional bronchoscopy treatment has been widely used to treat this disease in China.This study demonstrated the characteristics of scarring airway stenosis in Chinese adults and retrospectively evaluated the efficacy of interventional bronchoscopy treatment of this disease.Methods：Patients with scarring airway stenosis from 18 tertiary hospitals were enrolled between January 2013 and June 2016.The causes,site,and length of scarring airway stenosis were analyzed,and the efficacy of the interventional bronchoscopy treatment was evaluated.Results：The final study cohort consisted of 392 patients.Endotracheobronchial tuberculosis （EBTB） was the most common cause of scarring airway stenosis （305/392,77.8%） in Chinese adults with a high rate of incidence in young women.The left main bronchus was most susceptible to EBTB,and most posttuberculosis airway scarring stenosis length was 1.1-2.0 cm.The average clinical success rate of interventional bronchoscopy treatment for scarring airway stenosis in Chinese patients is 60.5%.The stent was inserted in 8.7% scarring airway stenosis in China.Conclusions：Scarring airway stenosis exhibits specific characteristics in Chinese patients.Interventional bronchoscopy is a useful and safe treatment method for the disease.

Rapid Growth of Halide Perovskite Single Crystals:From Methods to Optimization Control

Metal halide perovskites are emerging as new generation optoelectronic materials due to their high carrier mobility, long carrier diffusion length and large light absorption coefficient, which have broad applications in solar cell, light-emitting diode, laser, photodetector and transistors. Perovskite single crystal is an ideal platform for discerning the intrinsic properties of these materials. In some cases, perovskite single crystals are better candidates to gain high performance optoelectronics. However, the growth of perovskite single crystals is time and cost consuming, which has an obvious disadvantage for device exploration. Therefore, fast growth technique is highly desirable in not only promoting the use of perovskites in commercial applications but also facilitating deep physical investigation of the materials. In this review, we summarize thoroughly the development of fast growth of the halide perovskites single crystal. Specifically, we highlight the progress of rapid growth techniques with emphasis on the optimization control.

Comparison of clinical features and stent placement outcomes between airway stenosis caused by primary pulmonary malignancies and that caused by primary non-pulmonary malignancies

Background: Primary pulmonary malignancies (PPMs) and non-pulmonary malignancies (PNPMs) may result in airway stenosis requiring stenting. This study aimed to compare and evaluate the clinical features and stent placement outcomes of airway stenosis caused by PPMs and PNPMs. Methods: A total of 141 patients with malignant airway stenosis who underwent Micro-Tech stent placements between January 2004 and October 2017 at Department of Respiratory Medicine, Beijing Tian Tan Hospital, Capital Medical University were divided into PPM (n= 100) and PNPM groups (n = 41). Patients’ clinical features and stent placement outcomes were collected and analyzed. Chi-square test was used to compare the categorical variables, while independent- or paired-sample t test was used to compare the continuous variables. Results: There were no significant differences in age, sex, treatment history, respiratory symptoms, and incidence of obstructive pneumonia between groups. Multiple airway involvement (63.0% vs. 31.7%;χ^2=11.459, P= 0.001) and atelectasis (17.0% vs. 2.4%;χ^25.536, P= 0.019) were more common in the PPM group, while extraluminal obstruction (24.4% vs. 6.0%;χ^28.033, P= 0.005) was more common in the PNPM group. Before stenting, the American Thoracic Society Dyspnea Index (ADI) and Karnofsky Performance Scale (KPS) scores showed no significant differences between groups (all P > 0.05). After stenting, a satisfactory rate of symptom improvement was achieved in both groups (98.0% and 100.0% in the PPM and PNPM groups, respectively;χ^2 0.016, P= 0.898);ADI and KPS scores, which showed no significant differences between groups (all P > 0.05), were significantly improved in each group (all P < 0.001). Complications after stenting could be effectively managed using bronchoscopic procedures. Conclusions: Among cases of malignant airway stenosis requiring stenting, those caused by PPM are more likely to involve multiple airways and are associated with atelectasis, while those caused by PNPM are more likely to cause extraluminal obstruction. Micro- Tech stent placement has the same immediate effect in terms of improvement in respiratory symptoms and performance status for both malignant airway stenosis caused by PPM and that caused by PNPM.

Interventional Bronchoscopic Therapy in Adult Patients with Tracheobronchial Mucoepidermoid Carcinoma

Background： Tracheobronchial mucoepidermoid carcinoma （MEC） is a rare airway tumor in adults for which surgery is considered a first-line treatment. However, some patients already lost the best opportunity of a surgical intervention when diagnoses are confirmed, and surgery causes considerable trauma resulting in partial loss of pulmonary function. Moreover, the tumor is resistant to radiotherapy and chemotherapy. These factors make the treatment of tracheobronchial MEC challenging. This study aimed to evaluate the safety and et^cacy ofinterventional bronchoscopic therapy in adult patients with tracheobronchial MEC. Methods： We retrospectively analyzed the clinical manifestations, bronchoscopic interventions, complications, and outcomes of 11 adult patients with tracheobronchial MEC. Paired t-test was used to analyze the parameters of the American Thoracic Society Dyspnea Index and the Karnofsky Score before and after the first interventional bronchoscopic therapy. Results： All tumors occurred in the main bronchus and were easily visualized by bronchoscopy. After interventional bronchoscopic therapy, the symptoms of all patients showed significant improvement. The American Thoracic Society Dyspnea Index decreased from 1.91 ± 1.22 to 0.27 ± 0.47 （t = 6.708, P 〈 0.001） and the Kamofsky Score increased from 78.18 ±16.62 to 95.46 ± 8.20 （t =-5.190, P 〈 0.001 ）. Bronchoscopic intervention did not result in serious complications or mortality. During the follow-up period between 3 and 96 months after the first therapy, the following results were noted： （ 1 ） among the eight patients with low-grade tracheobronchial MEC, only one patient had a relapse and agreed to surgical treatment; （2） among the three patients with high-grade tracheobronchial MEC, one patient required repeated bronchoscopic interventions, one patient died of pulmonary infection, and one patient died of systemic failure owing to tumor metastasis. Conclusions： Interventional bronchoscopic therapy, as an alternative treatment, shows promise in some adult patients with low-grade tracheobronchial MEC confined to the bronchus. However, for adult patients with high-grade tracheobronchial MEC, early diagnosis and surgical treatment are still strongly recommended.

Paclitaxel Drug-eluting Tracheal Stent Could Reduce Granulation Tissue Formation in a Canine Model

Background：Currently available silicone and metallic stents for tracheal stenosis are associated with many problems.Granulation proliferation is one of the main complications.The present study aimed to evaluate the efficacy of paclitaxel drug-eluting tracheal stent in reducing granulation tissue formation in a canine model,as well as the pharmacokinetic features and safety profiles of the coated drug.Methods：Eight beagles were randomly divided into a control group （bare-metal stent group,n =4） and an experimental group （paclitaxel-eluting stent group,n =4）.The observation period was 5 months.One beagle in both groups was sacrificed at the end of the 1st and 3rd months,respectively.The last two beagles in both groups were sacrificed at the end of 5th month.The proliferation of granulation tissue and changes in tracheal mucosa were compared between the two groups.Blood routine and liver and kidney function were monitored to evaluate the safety of the paclitaxel-eluting stent.The elution method and high-performance liquid chromatography were used to characterize the rate of in vivo release of paclitaxel from the stent.Results：Compared with the control group,the proliferation of granulation tissue in the experimental group was significantly reduced.The drug release of paclitaxel-eluting stent was the fastest in the 1st month after implantation （up to 70.9%）.Then,the release slowed down gradually.By the 54 month,the release reached up to 98.5%.During the observation period,a high concentration of the drug in the trachea （in the stented and adjacent unstented areas） and lung tissue was not noted,and the blood test showed no side effect.Conclusions：The paclitaxel-eluting stent could safely reduce the granulation tissue formation after stent implantation in vivo,suggesting that the paclitaxel-eluting tracheal stent might be considered for potential use in humans in the future.

NaV3O8/poly(3,4-ethylenedioxythiophene) composites as high-rate and long-lifespan cathode materials for reversible sodium storage

Sodium-ion batteries have received a surge of interests for the alternatives to lithium-ion batteries due to their abundant reserves and low cost.The quest of reliable and high-performance cathode materials is crucial to future Na storage technologies.Herein,poly(3,4-ethylenedioxythiophene)(PEDOT)was successfully introduced to NaV3O8 via in situ oxidation polymerization,which can effectively enhance electron conductivity and ionic diffusion of NaV3O8 material.As a result,these NaV3O8@-PEDOT composites exhibit a significantly improved electrochemical performance including cycle stability and rate performance.In particular,NaV3O8@20 wt%PEDOT composite demonstrates better dispersibility and lower charge transfer resistance compared with bare NaV3O8,which delivers the first discharge capacity of 142 mAh-g-1and holds about 128.7 mAh·g-1 after 300 cycles at a current density of 120 mA·g-1.Even at a high current density of 300 mA·g-1,a high reversible capacity of 99.6 mAh·g-1 is revealed.All these consequences suggest that NaV3O8@20 wt%PEDOT composite may be a promising candidate to serve as a high-rate and long-lifespan cathode material for sodium-ion batteries.

Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

While some individuals infected by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)present mild-to-severe disease,many SARS-CoV-2-infected individuals are asymptomatic.We sought to identify the distinction of immune response between asymptomatic and moderate patients.We performed single-cell transcriptome and T-cell/B-cell receptor(TCR/BCR)sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic,moderate,and severe patients with healthy controls.Asymptomatic patients displayed increased CD56^(bri)CD16^(-) natural killer(NK)cells and upregulation of interferon-gamma in effector CD4^(+) and CD8^(+) T cells and NK cells.They showed more robust TCR clonal expansion,especially in effector CD4^(+) T cells,but lack strong BCR clonal expansion compared to moderate patients.Moreover,asymptomatic patients have lower interferon-stimulated genes(ISGs)expression in general but large interpatient variability,whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe disease.Our data provide evidenee of different immune signatures to SARS-CoV-2 in asymptomatic infections.

Fluorous Metal–Organic Frameworks with Unique Cage-in-Cage Structures Featuring Fluorophilic Pore Surfaces for Efficient C_(2)H_(2)/CO_(2)Separation

The similarities in molecular size and physical properties of acetylene(C_(2)H_(2))and carbon dioxide(CO_(2))produce a formidable challenge for their separation.Herein,we report two isoreticular fluorinated metal–organic frameworks(MOFs),labeled as JXNU-11(Fe2M)(M=Ni and Co),featuring unique octahedral cages encapsulated by cuboctahedral cages.JXNU-11(Fe_(2)Ni)shows a record high C_(2)H_(2)-capture amount of 4.8mmol g−1 and a long C_(2)H_(2)/CO_(2)breakthrough interval time of 55 min g^(−1)in an actual breakthrough experiment based on the equimolar C_(2)H_(2)/CO_(2)mixture under ambient conditions,indicating a high-performance material for C_(2)H_(2)capture andC_(2)H_(2)/CO_(2)separation.