As the core component of the rotating packing bed,packing is a place for efficient gas–liquid mixing and mass transfer.In this paper,a 3D structured packing composed of a mesh structure and a support structure was de...As the core component of the rotating packing bed,packing is a place for efficient gas–liquid mixing and mass transfer.In this paper,a 3D structured packing composed of a mesh structure and a support structure was designed.The mesh structure is a ring-shaped mesh surrounded by triangular meshes,which is stable in structure and can achieve a high degree of dispersion and aggregation of the liquid phase.The support structure is composed of ring-shaped structural units arranged at a certain angle along the axial direction,which can enhance the turbulence of the airflow while constructing regular gas-phase channels.Circumferential steel meshes of different diameters and supporting structures are alternately combined to form 3D packing,which is loaded in a layered cross-flow rotating packing bed.The results show that under the same operating conditions,the mass transfer performance of 3D packing and wire mesh packing are equivalent,and both are better than pall ring packing.Moreover,the pressure drop of 3D packing is significantly lower than that of pall ring packing and wire mesh packing.The design and implementation of packing the development presented in this paper can be used to develop special structured packing for rotating bed,which can further improve the performance of rotating packed bed(RPB).展开更多
BACKGROUND Systemic lupus erythematosus(SLE)is an autoimmune disease characterized by systemic involvement and multiple autoantibodies in the serum.Patients with protein C(PC)and protein S(PS)deficiency are prone to t...BACKGROUND Systemic lupus erythematosus(SLE)is an autoimmune disease characterized by systemic involvement and multiple autoantibodies in the serum.Patients with protein C(PC)and protein S(PS)deficiency are prone to thrombosis.In contrast,patients with primary hyperfibrino-lysis tend to bleed.CASE SUMMARY A 52-year-old female patient with bilateral pleural effusion was diagnosed with"tuberculous pleurisy"and treated with anti-tuberculosis drugs and prednisone.The coagulation-related laboratory results showed decreased fibrinogen,PC activity,PS activity,and antithrombinШactivity.The immune-related laboratory results showed positive antinuclear antibody,anti-Smith antibody,anticardiolipin antibody(ACL),anti-β2-glycoprotein I antibody(aβ2GPI)and direct Coomb’s test and decreased complement 3 and complement 4.Thoracoscopy was performed and bloody pleural fluid was drained.Pathology of the pleural biopsy showed lymphocytes,plasma cells,and a few eosinophils in adipose and fibrous connective tissue.Results of whole exome sequencing of blood showed no genetic mutations suggesting the presence of hereditary hematological diseases.The patient was finally diagnosed with SLE and primary hyperfibrinolysis,and was treated with prednisolone,hydroxychloroquine,and compound cyclophosphamide.CONCLUSION PC and PS deficiency in SLE might be related to ACL and aβ2GPI.SLE and primary hyperfibrinolysis can coexist in one patient,with both a risk of thrombosis and a risk of bleeding.展开更多
The shearing line is the key to improve the quality and efficiency of heavy plates.A model of contour recognition and intelligent shearing strategy for the heavy plate was proposed.Firstly,multi-array binocular vision...The shearing line is the key to improve the quality and efficiency of heavy plates.A model of contour recognition and intelligent shearing strategy for the heavy plate was proposed.Firstly,multi-array binocular vision linear cameras were used to complete the image acquisition.Secondly,the total length of the steel plate after cooling was predicted by back propagation neural network algorithm according to the contour data.Finally,using the scanning line and a new camber description method,the shearing strategy including head/tail irregular shape length and rough dividing strategy was calculated.The practical application shows that the model and strategy can effectively solve the problems existing in the shearing process and can effectively improve the yield of steel plates.The maximum error of detection width,length,camber,and the length of the irregular deformation area at the head/tail of the plate are all less than 5 mm.The correlation coefficient of the length prediction model based on the back propagation neural network is very high.The reverse ratio result of edge cutting failure using the proposed rough dividing strategy is 1/401=0.2%,which is 2%higher than that by human.展开更多
An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-52 cellulose and gel filtration chromatograp...An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-52 cellulose and gel filtration chromatography on Bio-Gel? P-100. The protein was absorbed on DEAE-cellulose and Bio-Gel? P-100. The purified antifungal fraction was designated as B29I, with a molecular mass of 42.3 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), pI value 5.69 by isoelectric focusing (IEF)-PAGE, and 97.81% purity by high performance liquid chromatography (HPLC). B29I exhibited inhibitory activity on mycelial growth in Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, and Sclerotinia sclerotiorum. The 50% inhibitory concentrations (IC50) of its antifungal activity toward Fusarium oxysporum and Rhizoctonia solani were 45 and 112 μmol/L, respectively. B29I also demonstrated an inhibitory effect on conidial spore germination of Fusarium oxysporum and suppression of germ-tube elongation, and induced distortion, tumescence, and rupture of a portion of the germinated spores.展开更多
Objective To compare clinical efficacy of three cervical preparations for surgical evacuation in first trimester missed abortion. Methods Two hundred and ten women with early missed abortion were equally randomized...Objective To compare clinical efficacy of three cervical preparations for surgical evacuation in first trimester missed abortion. Methods Two hundred and ten women with early missed abortion were equally randomized into three groups. Three cervical preparations, Dilapan-dilator method (group A, n= 70), mifepristone method (group B, n= 70), and misoprostol method (group C, n= 70), were used 24 h before curettage for terminating missed abortion, respectively. Clinical outcomes and complications for these three methods were compared. Results No differences were found in rates of successful treatment among the three groups (P〉O.05). The incidence of fitting number 8 Hegar dilator was slightly high in group A, but it was not different compared with that in group B or group C (P〉0.05). Bleeding in previous 24 h in group C was significantly greater than that in group A or group B (P〈0.01). The cases of blood loss (〉50 ml) during evacuation in group B were more than those in group A (P〉0.05). Women in group B had less abdominal pain than those in group A or group C (P〈0.01). Side effects in group C were more than those in group A on nausea (P〈0.01), vomiting (P〈0.05), and diarrhea (P〈0.01). Group B had the highest acceptability among the three groups (P〈0.05). Conclusion Three methods have the similar clinical efficacies in cervical ripening for surgical treatment of early missed abortion. Based on individual characteristics, different approach would be chosen to avoid some adverse events and improve the clinical application of Dilapan-dilator.展开更多
Objective To determine the role of the circulating antisperm antibody (ASA) in the pathogenesis of missed abortion at the first-trimester pregnancy. Methods Sixty-two patients with a history of missed abortion at th...Objective To determine the role of the circulating antisperm antibody (ASA) in the pathogenesis of missed abortion at the first-trimester pregnancy. Methods Sixty-two patients with a history of missed abortion at the first-trimester pregnancy were enrolled into this study. Indirect immunobead test (IBT) was used to measure the circulating ASA levels. Fifty healthy women with the first-trimester pregnancy set as the control. Results No case had the positive level of ASA according to the World Health Organization criteria (50% or more of the motile sperm with immunobead binding). Only 1 case in patient group and 1 case in the control had 10%-20% of the motile spermatozoa with ASA-IgG bead binding. In both patient and control groups, ASA-IgA was found to be completely negative binding. Conclusion The circulating ASA is not associated with the pathogenesis of missed abortions at the first-trimester pregnancy.展开更多
Objective To evaluate possible clinical effects of adiponectin, resistin, 1L-6, and TNF-a in obese and non-obese patients with polycystic ovary syndrome (PCOS). Methods Seventy selected PCOS patients were recruited ...Objective To evaluate possible clinical effects of adiponectin, resistin, 1L-6, and TNF-a in obese and non-obese patients with polycystic ovary syndrome (PCOS). Methods Seventy selected PCOS patients were recruited for this study, and were divided into two groups based on their body mass index (BM1): 35 obese (BM1 25 kg/m2, group A), 35 non-obese (BMI〈25 kg/me, group B). In addition, 35 healthy non-obese women (BMI〈25 kg/m2) were enrolled as the control (group C). Serum levels of FSH, LH, T, glucose, insulin, adiponectin, resistin, 1L-6, TNF-a. were detected, and compared the differences of them among three groups. Results Blood glucose levels among three groups had no difference (P〉0.05). Blood insulin level was significantly greater in group A than in group B or group C (P〈0.05), and a significant difference existed between group B and group C (P〈0. 05). The ratio of glucose/insulin was significantly higher in group A than in group B or group C (P〈0.05), and the ratio in group B was significantly higher than that in group C (P〈0.05). Adiponectin level was significantly lower and resistin level was significantly higher in group A than in group B or group C (P〈0.05). IL-6 level in group C was significantly lower than that in group A or group B (P〈0.05), and significant difference was found between group A and group B (P〈0.05). TNF-a level was a slight high in group B, whereas there was no statistical difference among three groups (P〉0.05). Conclusion Disturbances of some metabolic and inflammatory adipokines could involve the pathogenesis of PCOS in both obese and non-obese women. Low-grade chronic inflammation might have negative effects on the development of PCOS in non- obese women.展开更多
The influence of magnetic interactions to the magnetization dynamics was well experimentally studied in a 3d-4f single-molecule magnet(SMM)[Tb^Ⅲ2Fe^Ⅲ3(μ5-O)L2(NO3)4Cl](1,H4L=N,N,N′,N′-tetrakis(2-hydroxyethyl)ethy...The influence of magnetic interactions to the magnetization dynamics was well experimentally studied in a 3d-4f single-molecule magnet(SMM)[Tb^Ⅲ2Fe^Ⅲ3(μ5-O)L2(NO3)4Cl](1,H4L=N,N,N′,N′-tetrakis(2-hydroxyethyl)ethylene diamine)and its diamagneticion-diluted samples.Significant ferromagnetic coupling between Tb^Ⅲ and Fe^Ⅲ ions and SMM behavior of 1 were observable,which proved clearly that the magnetic interaction between 3d-4f spin carriers has also an excessive impact on fine-tuning the magnetization dynamic behaviors of 3d-4f complexes.展开更多
Objective To compare clinical efficacy for two cervical preparations for early secondtrimester pregnancy termination at 12-17 weeks gestation. Methods Seventy healthy women aged 18-41 years requesting legal terminatio...Objective To compare clinical efficacy for two cervical preparations for early secondtrimester pregnancy termination at 12-17 weeks gestation. Methods Seventy healthy women aged 18-41 years requesting legal termination of pregnancy at 12-17 weeks of gestation were equally randomized into two groups. Two cervical preparations, the Dilapan-STM combined with mifepristone and misoprostol (DMM) method, and the mifepristone combined with misoprostol (MM) method, were used to end early second-trimester pregnancy for group DMM and group MM, respectively. Clinical outcomes and complications for these two methods were compared. Results No differences were found on rates of successful abortion within 48 h and blood loss after abortion at 2 h between the two groups (P〉0.05). DMM group had significant shorter induction-to-abortion interval and hospital stay compared with group MM (P〈0.01). In both DMM and MM groups, side effects including nausea/vomiting, diarrhea, chills and rigors, and fever occurred, and incidences of these side effects were no difference (P〉0.05). Conclusion DMM method would have similar clinical efficacies with MM method for early second-trimester pregnancy termination. Besides,DMM method has shorter induction-to-abortion interval and hospital stay, which benefits its clinical application.展开更多
Objective To evaluate levels of the circulating antisperm antibody (ASA) in women with secondary infertility. Methods Eighty-three women defined as secondary infertility were enrolled into this study. Based on their...Objective To evaluate levels of the circulating antisperm antibody (ASA) in women with secondary infertility. Methods Eighty-three women defined as secondary infertility were enrolled into this study. Based on their outcomes of previous pregnancy, these women were divided into two groups: group HC (having at least one child, n=45), group NC (no child but one to three abortions, n=35). The serum was screened by the indirect immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. Results Of the 83 patients assessed for ASA-IgG, 14 had immunobead binding rate ranged from 15% to 43%, and the sub-positive rate was 16.9%. Five cases showed ASA-IgA sub-positive, and had binding rate between 10% and 20%. No case had the clinical positive level according to the WHO criteria. No differences were found on ASA-IgG and ASA-IgA between HC and NC groups (P〉0.05). Conclusion The circulating ASA may be a possible cause to decrease fertility in women with secondary infertility.展开更多
Objective To evaluate effects of rosiglitazone (RSG) on the expression of adiponectin in mature adipocytes at high levels of both testosterone (T) and insulin in vitro culture. Methods Mouse 3T3-L1 preadipocytes w...Objective To evaluate effects of rosiglitazone (RSG) on the expression of adiponectin in mature adipocytes at high levels of both testosterone (T) and insulin in vitro culture. Methods Mouse 3T3-L1 preadipocytes were induced to be mature adipocytes and used in this study. According to RSG concentrations, the cells added with T (10^-5 mol/L) and insulin (10^-4 mol/L) were divided into 4 groups: free-RSG group (0 mol/L RSG, FR-TI), low-dose group (10^-9 mol/L RSG, LR-TI), middle-dose group (lO-7mol/L RSG, MR-T1) and high-dose group (10^-6 mol/L RSG, HR-TI). Besides, the cells added with RSG without T and insulin were also divided into 4 groups: FR, LR, MR, and HR. These 8 groups were incubated for 42 h. Cell viability was determined by MTT assay. Expression of adiponectin was detected by Western blotting. Results The maximum viability in FR-TI group was observed at point of 42 h. The growth of the adipocytes was significantly inhibited in MR-TI group compared with FR-TI (P〈0.01). The level of adiponectin in MR-TI group was higher than that in LR- TI group (P〈0.01). However, with RSG increasing; HR-TI group showed the lowest level of adiponectin among three treatment groups (P〈0.01). In addition, adiponectin expression in MR-TI group was significantly higher than that in MR group (P〈0.01). Conclusion RSG could increase the expression of adiponectin in 3T3-L1 adipocytes under high levels of both T and insulin, but it acts in a narrow concentration range.展开更多
基金Project Supported by Shanxi Scholarship Council of China(2021-120)the National Natural Science Foundation of China International(Regional)Cooperation and Exchange Project(21961160740)。
文摘As the core component of the rotating packing bed,packing is a place for efficient gas–liquid mixing and mass transfer.In this paper,a 3D structured packing composed of a mesh structure and a support structure was designed.The mesh structure is a ring-shaped mesh surrounded by triangular meshes,which is stable in structure and can achieve a high degree of dispersion and aggregation of the liquid phase.The support structure is composed of ring-shaped structural units arranged at a certain angle along the axial direction,which can enhance the turbulence of the airflow while constructing regular gas-phase channels.Circumferential steel meshes of different diameters and supporting structures are alternately combined to form 3D packing,which is loaded in a layered cross-flow rotating packing bed.The results show that under the same operating conditions,the mass transfer performance of 3D packing and wire mesh packing are equivalent,and both are better than pall ring packing.Moreover,the pressure drop of 3D packing is significantly lower than that of pall ring packing and wire mesh packing.The design and implementation of packing the development presented in this paper can be used to develop special structured packing for rotating bed,which can further improve the performance of rotating packed bed(RPB).
文摘BACKGROUND Systemic lupus erythematosus(SLE)is an autoimmune disease characterized by systemic involvement and multiple autoantibodies in the serum.Patients with protein C(PC)and protein S(PS)deficiency are prone to thrombosis.In contrast,patients with primary hyperfibrino-lysis tend to bleed.CASE SUMMARY A 52-year-old female patient with bilateral pleural effusion was diagnosed with"tuberculous pleurisy"and treated with anti-tuberculosis drugs and prednisone.The coagulation-related laboratory results showed decreased fibrinogen,PC activity,PS activity,and antithrombinШactivity.The immune-related laboratory results showed positive antinuclear antibody,anti-Smith antibody,anticardiolipin antibody(ACL),anti-β2-glycoprotein I antibody(aβ2GPI)and direct Coomb’s test and decreased complement 3 and complement 4.Thoracoscopy was performed and bloody pleural fluid was drained.Pathology of the pleural biopsy showed lymphocytes,plasma cells,and a few eosinophils in adipose and fibrous connective tissue.Results of whole exome sequencing of blood showed no genetic mutations suggesting the presence of hereditary hematological diseases.The patient was finally diagnosed with SLE and primary hyperfibrinolysis,and was treated with prednisolone,hydroxychloroquine,and compound cyclophosphamide.CONCLUSION PC and PS deficiency in SLE might be related to ACL and aβ2GPI.SLE and primary hyperfibrinolysis can coexist in one patient,with both a risk of thrombosis and a risk of bleeding.
基金The paper was prepared under the support of the Natural Science Foundation of Liaoning Province(Grant No.2022-MS-277)This research was also financially supported by the Youth Project of Foundation of Liaoning Province Education Administration(Grant No.lnqn202016).
文摘The shearing line is the key to improve the quality and efficiency of heavy plates.A model of contour recognition and intelligent shearing strategy for the heavy plate was proposed.Firstly,multi-array binocular vision linear cameras were used to complete the image acquisition.Secondly,the total length of the steel plate after cooling was predicted by back propagation neural network algorithm according to the contour data.Finally,using the scanning line and a new camber description method,the shearing strategy including head/tail irregular shape length and rough dividing strategy was calculated.The practical application shows that the model and strategy can effectively solve the problems existing in the shearing process and can effectively improve the yield of steel plates.The maximum error of detection width,length,camber,and the length of the irregular deformation area at the head/tail of the plate are all less than 5 mm.The correlation coefficient of the length prediction model based on the back propagation neural network is very high.The reverse ratio result of edge cutting failure using the proposed rough dividing strategy is 1/401=0.2%,which is 2%higher than that by human.
基金supported by the Hi-Tech Research and Development Pro-gram (863) of China (No. 2003AA241140)the Natural Science Foundation of Heilongjiang Province, China (No. C200522)
文摘An antifungal protein was isolated from a culture of Bacillus subtilis strain B29. The isolation procedure comprised ion exchange chromatography on diethylaminoethyl (DEAE)-52 cellulose and gel filtration chromatography on Bio-Gel? P-100. The protein was absorbed on DEAE-cellulose and Bio-Gel? P-100. The purified antifungal fraction was designated as B29I, with a molecular mass of 42.3 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), pI value 5.69 by isoelectric focusing (IEF)-PAGE, and 97.81% purity by high performance liquid chromatography (HPLC). B29I exhibited inhibitory activity on mycelial growth in Fusarium oxysporum, Rhizoctonia solani, Fusarium moniliforme, and Sclerotinia sclerotiorum. The 50% inhibitory concentrations (IC50) of its antifungal activity toward Fusarium oxysporum and Rhizoctonia solani were 45 and 112 μmol/L, respectively. B29I also demonstrated an inhibitory effect on conidial spore germination of Fusarium oxysporum and suppression of germ-tube elongation, and induced distortion, tumescence, and rupture of a portion of the germinated spores.
基金supported by Science and Technology Planning Project of Guangdong Province,China (No. 2010B031600250)
文摘Objective To compare clinical efficacy of three cervical preparations for surgical evacuation in first trimester missed abortion. Methods Two hundred and ten women with early missed abortion were equally randomized into three groups. Three cervical preparations, Dilapan-dilator method (group A, n= 70), mifepristone method (group B, n= 70), and misoprostol method (group C, n= 70), were used 24 h before curettage for terminating missed abortion, respectively. Clinical outcomes and complications for these three methods were compared. Results No differences were found in rates of successful treatment among the three groups (P〉O.05). The incidence of fitting number 8 Hegar dilator was slightly high in group A, but it was not different compared with that in group B or group C (P〉0.05). Bleeding in previous 24 h in group C was significantly greater than that in group A or group B (P〈0.01). The cases of blood loss (〉50 ml) during evacuation in group B were more than those in group A (P〉0.05). Women in group B had less abdominal pain than those in group A or group C (P〈0.01). Side effects in group C were more than those in group A on nausea (P〈0.01), vomiting (P〈0.05), and diarrhea (P〈0.01). Group B had the highest acceptability among the three groups (P〈0.05). Conclusion Three methods have the similar clinical efficacies in cervical ripening for surgical treatment of early missed abortion. Based on individual characteristics, different approach would be chosen to avoid some adverse events and improve the clinical application of Dilapan-dilator.
文摘Objective To determine the role of the circulating antisperm antibody (ASA) in the pathogenesis of missed abortion at the first-trimester pregnancy. Methods Sixty-two patients with a history of missed abortion at the first-trimester pregnancy were enrolled into this study. Indirect immunobead test (IBT) was used to measure the circulating ASA levels. Fifty healthy women with the first-trimester pregnancy set as the control. Results No case had the positive level of ASA according to the World Health Organization criteria (50% or more of the motile sperm with immunobead binding). Only 1 case in patient group and 1 case in the control had 10%-20% of the motile spermatozoa with ASA-IgG bead binding. In both patient and control groups, ASA-IgA was found to be completely negative binding. Conclusion The circulating ASA is not associated with the pathogenesis of missed abortions at the first-trimester pregnancy.
基金supported by the Fundamental Research Funds for the Central Universities,China(No.21612445)
文摘Objective To evaluate possible clinical effects of adiponectin, resistin, 1L-6, and TNF-a in obese and non-obese patients with polycystic ovary syndrome (PCOS). Methods Seventy selected PCOS patients were recruited for this study, and were divided into two groups based on their body mass index (BM1): 35 obese (BM1 25 kg/m2, group A), 35 non-obese (BMI〈25 kg/me, group B). In addition, 35 healthy non-obese women (BMI〈25 kg/m2) were enrolled as the control (group C). Serum levels of FSH, LH, T, glucose, insulin, adiponectin, resistin, 1L-6, TNF-a. were detected, and compared the differences of them among three groups. Results Blood glucose levels among three groups had no difference (P〉0.05). Blood insulin level was significantly greater in group A than in group B or group C (P〈0.05), and a significant difference existed between group B and group C (P〈0. 05). The ratio of glucose/insulin was significantly higher in group A than in group B or group C (P〈0.05), and the ratio in group B was significantly higher than that in group C (P〈0.05). Adiponectin level was significantly lower and resistin level was significantly higher in group A than in group B or group C (P〈0.05). IL-6 level in group C was significantly lower than that in group A or group B (P〈0.05), and significant difference was found between group A and group B (P〈0.05). TNF-a level was a slight high in group B, whereas there was no statistical difference among three groups (P〉0.05). Conclusion Disturbances of some metabolic and inflammatory adipokines could involve the pathogenesis of PCOS in both obese and non-obese women. Low-grade chronic inflammation might have negative effects on the development of PCOS in non- obese women.
基金the National Natural Science Foundation of China(Nos.21622105,21331003 and 21421001)the Natural Science Foundation of Tianjin(No.18JCJQJC47200)the Ministry of Education of China(No.B12015).
文摘The influence of magnetic interactions to the magnetization dynamics was well experimentally studied in a 3d-4f single-molecule magnet(SMM)[Tb^Ⅲ2Fe^Ⅲ3(μ5-O)L2(NO3)4Cl](1,H4L=N,N,N′,N′-tetrakis(2-hydroxyethyl)ethylene diamine)and its diamagneticion-diluted samples.Significant ferromagnetic coupling between Tb^Ⅲ and Fe^Ⅲ ions and SMM behavior of 1 were observable,which proved clearly that the magnetic interaction between 3d-4f spin carriers has also an excessive impact on fine-tuning the magnetization dynamic behaviors of 3d-4f complexes.
基金supported by Science and Technology Planning Project of Guangdong Province, China (No. 2010B031600250)Scientific and Technical Personnel Serving Company in Guangdong Action Items, China (No. 2009GJE00008)
文摘Objective To compare clinical efficacy for two cervical preparations for early secondtrimester pregnancy termination at 12-17 weeks gestation. Methods Seventy healthy women aged 18-41 years requesting legal termination of pregnancy at 12-17 weeks of gestation were equally randomized into two groups. Two cervical preparations, the Dilapan-STM combined with mifepristone and misoprostol (DMM) method, and the mifepristone combined with misoprostol (MM) method, were used to end early second-trimester pregnancy for group DMM and group MM, respectively. Clinical outcomes and complications for these two methods were compared. Results No differences were found on rates of successful abortion within 48 h and blood loss after abortion at 2 h between the two groups (P〉0.05). DMM group had significant shorter induction-to-abortion interval and hospital stay compared with group MM (P〈0.01). In both DMM and MM groups, side effects including nausea/vomiting, diarrhea, chills and rigors, and fever occurred, and incidences of these side effects were no difference (P〉0.05). Conclusion DMM method would have similar clinical efficacies with MM method for early second-trimester pregnancy termination. Besides,DMM method has shorter induction-to-abortion interval and hospital stay, which benefits its clinical application.
文摘Objective To evaluate levels of the circulating antisperm antibody (ASA) in women with secondary infertility. Methods Eighty-three women defined as secondary infertility were enrolled into this study. Based on their outcomes of previous pregnancy, these women were divided into two groups: group HC (having at least one child, n=45), group NC (no child but one to three abortions, n=35). The serum was screened by the indirect immunobead test for ASA type IgG and IgA according to the WHO laboratory manual. Results Of the 83 patients assessed for ASA-IgG, 14 had immunobead binding rate ranged from 15% to 43%, and the sub-positive rate was 16.9%. Five cases showed ASA-IgA sub-positive, and had binding rate between 10% and 20%. No case had the clinical positive level according to the WHO criteria. No differences were found on ASA-IgG and ASA-IgA between HC and NC groups (P〉0.05). Conclusion The circulating ASA may be a possible cause to decrease fertility in women with secondary infertility.
基金supported by Science and Technology Planning Project of Guangdong Province,China(No.2012B031800400)
文摘Objective To evaluate effects of rosiglitazone (RSG) on the expression of adiponectin in mature adipocytes at high levels of both testosterone (T) and insulin in vitro culture. Methods Mouse 3T3-L1 preadipocytes were induced to be mature adipocytes and used in this study. According to RSG concentrations, the cells added with T (10^-5 mol/L) and insulin (10^-4 mol/L) were divided into 4 groups: free-RSG group (0 mol/L RSG, FR-TI), low-dose group (10^-9 mol/L RSG, LR-TI), middle-dose group (lO-7mol/L RSG, MR-T1) and high-dose group (10^-6 mol/L RSG, HR-TI). Besides, the cells added with RSG without T and insulin were also divided into 4 groups: FR, LR, MR, and HR. These 8 groups were incubated for 42 h. Cell viability was determined by MTT assay. Expression of adiponectin was detected by Western blotting. Results The maximum viability in FR-TI group was observed at point of 42 h. The growth of the adipocytes was significantly inhibited in MR-TI group compared with FR-TI (P〈0.01). The level of adiponectin in MR-TI group was higher than that in LR- TI group (P〈0.01). However, with RSG increasing; HR-TI group showed the lowest level of adiponectin among three treatment groups (P〈0.01). In addition, adiponectin expression in MR-TI group was significantly higher than that in MR group (P〈0.01). Conclusion RSG could increase the expression of adiponectin in 3T3-L1 adipocytes under high levels of both T and insulin, but it acts in a narrow concentration range.