We give the moment expressions for the boson resonances X with spin-parity JXPXC=0++,1-+,1++ and 2++ posibly produced in the process J/ψ→ρX，X→b1(1235)π，b1→ωπ in terms of the generalized moment analysis metho...We give the moment expressions for the boson resonances X with spin-parity JXPXC=0++,1-+,1++ and 2++ posibly produced in the process J/ψ→ρX，X→b1(1235)π，b1→ωπ in terms of the generalized moment analysis method .The resonance with JXPXC=1-+ can e distingguished from other resonances by means of these moments exceptfor some rather special cases.The suggestion that the search for the hybrid with IG(JPC)=1-(1-+) can be performed in the decay channl J/ψ→ρωππat upgraded BEPC/BES is presented.展开更多
AIM:To assess the variability of adhesin gene hpaA between different Helicobacter pylori (H pylori) strains with PCR-restriction fragment length polymorphism (RFLP). METHODS: Twelve different H pylori strains were cho...AIM:To assess the variability of adhesin gene hpaA between different Helicobacter pylori (H pylori) strains with PCR-restriction fragment length polymorphism (RFLP). METHODS: Twelve different H pylori strains were chosen to amplify the 710-bp segments of gene hpaA. These strains were NCTC11637, SS1; Chongqing clinical isolates CCS9801, CCS9802, CCS9803, CCS9806, CCS9809, CCS9810, CCS9813, which were gained from patients of gastritis; Mongolia gerbil adapted H pylori strains (abbreviation MG), which were gained from the following steps: gastric mucosal specimens of Mongolia gerbils infected by clinical isolate CCS9803 were cultured and detected, the positive H pylori strains were named as the first generation of Mongolia gerbil adapted H pylori strains (abbreviation MG1) and then were subcultured with healthy Mongolia gerbil to generate MG2, in turn to gain the ninth generation (abbreviation MG9). All hpaA segments, obtained from 12 different H pylori strains, were digested by Hhal and HaeIll individually and analyzed by agarose gel electrophoresis. RESULTS: In all 12 strains, the 710-bp PCR products were successfully amplified and products were cloned to pMD18-T vector respectively, then the recombinant plasmids were digested simultaneously with Ncol and Xhol to recover the small fragments. The objective fragments from 12 different H pylori strains digested with Haelll could be seen as 4 types of bands and 5 types with Hha I. According to the hpaA RFLP patterns, the 12 H pylori strains could be divided into 5 groups: group I, NCTC11637 and SS1; group II, CCS9809, which RFLP type digested with HaeIll was the same as strains of group I, but Hhal RFLP showed difference compared with the other groups; group III, CCS9810; group IV, CCS9803; group V: CCS9801, CCS9802, CCS9806, CCS9813, MGl, MG3 and MG9. The sequence data of 12 hpaA segments were analyzed by DNAsis software and it was observed that: (1) The homologies of base pair and amino acid sequence between strains NCTC11637, SS1, CCS9809 were 99.6% and 98.9%, respectively; (2) The homology of base pair and amino acid sequence between CCS9803 and CCS9810 was 97.7% and 99.1%; (3) That of the rest strains, CCS9801, CCS9802, CCS9806, CCS9813, MGl, MG3, MG9 reached 99.4% and 98.4%; (4) The base pair homologies between all hpaA fragments of different sources were higher than 94.6%, therefore the correspondence of deduced amino acid sequence was higher than 96.8% between each other. CONCLUSION: The gene hpaA from different H pylori strains revealed variation, and this might provide an effective method for molecular epidemiological survey of H pylori.展开更多
The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of macrophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of e...The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of macrophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot Analysis. Enzymatic activity of MMP-12 was determined by β-casein zymography. RT-PCR analysis was used to measure the mRNA expression level of MMP-12. OxLDL-stimulated macrophages produced increased casein-degrading activities and oxLDL also significantly increased the mRNA level of MMP-12 in a dose-dependent manner. OxLDL stimulated the phosphorylation of ERK1/2 in macrophages. The use of the specific inhibitor indicated that the ERK1/2 signaling pathway was required for the induction of MMP-12. These data demonstrated that oxLDL induced MMP-12 expression in macrophages through an ERK1/2-dependent pathway. Key words oxidized low density lipoproteins - macrophage - matrix metalloproteinases CLC number Q 556+.9 Foundation item: Supported by the Natural Science Foundation of Hubei Province (99J138)Biography: He Chun-yan (1970-), female, Ph. D candidate, research direction: biochemistry diagnosis of atherosclerosis.展开更多
A novel MEMS device boning system is presented. Aiming at the high velocity, high precision and high flexibility requirements, a novel manipulator of planar parallel structure is developed to substitute ordinary X-Y t...A novel MEMS device boning system is presented. Aiming at the high velocity, high precision and high flexibility requirements, a novel manipulator of planar parallel structure is developed to substitute ordinary X-Y table. In addition, the machine vision is implemented to improve the system＇ s flexibility. The initial angular positions of the joints are estimated by the extended Kalman filter algorithm. As a resuh, the manipulator＇s absolute locating accuracy in its workspace is guaranteed indirectly. For any MEMS device, the bonding system itself can be used as measurement equipment to create the device＇ s geometry model, which is the base to do off-line programming. A quite ideal trade-off between the system＇ s flexibility and efficiency is got. Finally, some verified motion specification of the manipulator, the bonding experimental results and the verified qualities of the bonded devices are provided.展开更多
Wu Yuhong published an article on the animals issued to the 6-uz-ga institution and its two cooks, in addition to two articles on the Calendar starting from the Month of Akiti in Puzris-Dagan, in 1996, 2000 and 2002. ...Wu Yuhong published an article on the animals issued to the 6-uz-ga institution and its two cooks, in addition to two articles on the Calendar starting from the Month of Akiti in Puzris-Dagan, in 1996, 2000 and 2002. From 2006, more and more Puzris-Dagan texts have been available to us through BDTNS and CDLI online, and this situation urged us to revise and make a complete archive of the four pairs of cooks （muhaldim） working in the e-uz-ga dining hall,展开更多
Rough Set is a valid mathematical theory developed in recent years, which has been applied successfully in such fields as machine learning, data mining, intelligent data analyzing and control algorithm acquiring. In t...Rough Set is a valid mathematical theory developed in recent years, which has been applied successfully in such fields as machine learning, data mining, intelligent data analyzing and control algorithm acquiring. In this paper, the authors discuss the reduction of knowledge using conditional entropy in rough set theory. First, the changing tendency of the conditional entropy of decision attributes giving condition attributes is studied from the viewpoint of information. Next, a new reduction algorithm based on conditional entropy is developed. Furthermore, our simulation results show that the algorithm can find the minimal reduction in most cases.展开更多
Rough Set is a valid mathematical theory developed in recent years, which hasthe ability to deal with imprecise, uncertain, and vague information. This paper presents a newincremental rule acquisition algorithm based ...Rough Set is a valid mathematical theory developed in recent years, which hasthe ability to deal with imprecise, uncertain, and vague information. This paper presents a newincremental rule acquisition algorithm based on rough set theory. First, the relation of the newinstances with the original rule set is discussed. Then the change law of attribute reduction andvalue reduction are studied when a new instance is added. Follow, a new incremental learningalgorithm for decision tables is presented within the framework of rough set. Finally, the newalgorithm and the classical algorithm are analyzed and compared by theory and experiments.展开更多
文摘We give the moment expressions for the boson resonances X with spin-parity JXPXC=0++,1-+,1++ and 2++ posibly produced in the process J/ψ→ρX，X→b1(1235)π，b1→ωπ in terms of the generalized moment analysis method .The resonance with JXPXC=1-+ can e distingguished from other resonances by means of these moments exceptfor some rather special cases.The suggestion that the search for the hybrid with IG(JPC)=1-(1-+) can be performed in the decay channl J/ψ→ρωππat upgraded BEPC/BES is presented.
基金Supported by the Major Projects of the National High Technology R&D (863) Program of China, No. 2001AA215161
文摘AIM:To assess the variability of adhesin gene hpaA between different Helicobacter pylori (H pylori) strains with PCR-restriction fragment length polymorphism (RFLP). METHODS: Twelve different H pylori strains were chosen to amplify the 710-bp segments of gene hpaA. These strains were NCTC11637, SS1; Chongqing clinical isolates CCS9801, CCS9802, CCS9803, CCS9806, CCS9809, CCS9810, CCS9813, which were gained from patients of gastritis; Mongolia gerbil adapted H pylori strains (abbreviation MG), which were gained from the following steps: gastric mucosal specimens of Mongolia gerbils infected by clinical isolate CCS9803 were cultured and detected, the positive H pylori strains were named as the first generation of Mongolia gerbil adapted H pylori strains (abbreviation MG1) and then were subcultured with healthy Mongolia gerbil to generate MG2, in turn to gain the ninth generation (abbreviation MG9). All hpaA segments, obtained from 12 different H pylori strains, were digested by Hhal and HaeIll individually and analyzed by agarose gel electrophoresis. RESULTS: In all 12 strains, the 710-bp PCR products were successfully amplified and products were cloned to pMD18-T vector respectively, then the recombinant plasmids were digested simultaneously with Ncol and Xhol to recover the small fragments. The objective fragments from 12 different H pylori strains digested with Haelll could be seen as 4 types of bands and 5 types with Hha I. According to the hpaA RFLP patterns, the 12 H pylori strains could be divided into 5 groups: group I, NCTC11637 and SS1; group II, CCS9809, which RFLP type digested with HaeIll was the same as strains of group I, but Hhal RFLP showed difference compared with the other groups; group III, CCS9810; group IV, CCS9803; group V: CCS9801, CCS9802, CCS9806, CCS9813, MGl, MG3 and MG9. The sequence data of 12 hpaA segments were analyzed by DNAsis software and it was observed that: (1) The homologies of base pair and amino acid sequence between strains NCTC11637, SS1, CCS9809 were 99.6% and 98.9%, respectively; (2) The homology of base pair and amino acid sequence between CCS9803 and CCS9810 was 97.7% and 99.1%; (3) That of the rest strains, CCS9801, CCS9802, CCS9806, CCS9813, MGl, MG3, MG9 reached 99.4% and 98.4%; (4) The base pair homologies between all hpaA fragments of different sources were higher than 94.6%, therefore the correspondence of deduced amino acid sequence was higher than 96.8% between each other. CONCLUSION: The gene hpaA from different H pylori strains revealed variation, and this might provide an effective method for molecular epidemiological survey of H pylori.
文摘The present study was undertaken to investigate the effect of oxidized low density lipoprotein (oxLDL) on the expression of macrophage matrix metalloproteinase-12 (MMP-12), and the possible mechanisms. Activation of extracellular signal-regulated kinase 1/2 (ERK1/2) was detected by Western blot Analysis. Enzymatic activity of MMP-12 was determined by β-casein zymography. RT-PCR analysis was used to measure the mRNA expression level of MMP-12. OxLDL-stimulated macrophages produced increased casein-degrading activities and oxLDL also significantly increased the mRNA level of MMP-12 in a dose-dependent manner. OxLDL stimulated the phosphorylation of ERK1/2 in macrophages. The use of the specific inhibitor indicated that the ERK1/2 signaling pathway was required for the induction of MMP-12. These data demonstrated that oxLDL induced MMP-12 expression in macrophages through an ERK1/2-dependent pathway. Key words oxidized low density lipoproteins - macrophage - matrix metalloproteinases CLC number Q 556+.9 Foundation item: Supported by the Natural Science Foundation of Hubei Province (99J138)Biography: He Chun-yan (1970-), female, Ph. D candidate, research direction: biochemistry diagnosis of atherosclerosis.
基金Supported by the High Technology Research and Development Programme of China （No. 2003AA404060） and the National Natural Science Foundation of China （No.60405008）.
文摘A novel MEMS device boning system is presented. Aiming at the high velocity, high precision and high flexibility requirements, a novel manipulator of planar parallel structure is developed to substitute ordinary X-Y table. In addition, the machine vision is implemented to improve the system＇ s flexibility. The initial angular positions of the joints are estimated by the extended Kalman filter algorithm. As a resuh, the manipulator＇s absolute locating accuracy in its workspace is guaranteed indirectly. For any MEMS device, the bonding system itself can be used as measurement equipment to create the device＇ s geometry model, which is the base to do off-line programming. A quite ideal trade-off between the system＇ s flexibility and efficiency is got. Finally, some verified motion specification of the manipulator, the bonding experimental results and the verified qualities of the bonded devices are provided.
基金supported by the Noble Group Fundthe W.F.Albright Institute of Archaeological ResearchJerusalem
文摘Wu Yuhong published an article on the animals issued to the 6-uz-ga institution and its two cooks, in addition to two articles on the Calendar starting from the Month of Akiti in Puzris-Dagan, in 1996, 2000 and 2002. From 2006, more and more Puzris-Dagan texts have been available to us through BDTNS and CDLI online, and this situation urged us to revise and make a complete archive of the four pairs of cooks （muhaldim） working in the e-uz-ga dining hall,
文摘Rough Set is a valid mathematical theory developed in recent years, which has been applied successfully in such fields as machine learning, data mining, intelligent data analyzing and control algorithm acquiring. In this paper, the authors discuss the reduction of knowledge using conditional entropy in rough set theory. First, the changing tendency of the conditional entropy of decision attributes giving condition attributes is studied from the viewpoint of information. Next, a new reduction algorithm based on conditional entropy is developed. Furthermore, our simulation results show that the algorithm can find the minimal reduction in most cases.
基金This work is supported by National Science Foundation of China (No.60373111).
文摘Rough Set is a valid mathematical theory developed in recent years, which hasthe ability to deal with imprecise, uncertain, and vague information. This paper presents a newincremental rule acquisition algorithm based on rough set theory. First, the relation of the newinstances with the original rule set is discussed. Then the change law of attribute reduction andvalue reduction are studied when a new instance is added. Follow, a new incremental learningalgorithm for decision tables is presented within the framework of rough set. Finally, the newalgorithm and the classical algorithm are analyzed and compared by theory and experiments.
