The first local case of mpox caused by an imported case in the Chinese mainland

Monkeypox (mpox) is a zoonotic disease caused by the mpox virus (MPXV) that has been primarily limited to Central and West African nations since its discovery. The recent spread of the West African lineage of MPXV in historically unaffected countries has raised concerns for global public health. Despite a significant decrease in global mpox cases, there is still a risk of a global resurgence. This study reports the first local case of mpox caused by an imported case in the Chinese mainland. Polymerase chain reaction (PCR) diagnosed the two cases, and the viral genomes were obtained by next-generation sequencing. Genomic analysis revealed that the two strains shared an identical genome sequence and belonged to the B.1.3 branch of the West African lineage, which is the first local case of mpox caused by an imported case in the Chinese mainland, highlighting the potential threat of mpox in China and the immediate need for adequate surveillance measures.

Genetic Features of 84 Genomes of Monkeypox Virus in Recent Circulation-Beijing Municipality,China,2023

The first indigenous incidence of Mpox(previously known as monkeypox)within Chinese mainland was documented in May 2023,with subsequent local and imported cases identified.A comprehensive understanding of the Mpox virus’s(MPXV)characteristics within Beijing remains incomplete.In this study,84 MPXV genomes from 82 local incidents and two imported instances,detected between May and July 2023,were analyzed.All MPXV strains fell within lineage C.1 of the West African clade,displaying limited genetic heterogeneity,encompassing 76–87 nucleotide substitutions and holding nucleotide identities between 99.996%and 100%.Phylogenetic exploration indicated that all genomes exhibited high homology to those presently prevalent in neighboring East Asian and Southeast Asian regions.Forty-six distinct haplotypes were identified among the strains,with 36.90%of genomes corresponding to four common haplotypes,suggesting repeated cross-regional introductions and restrained distribution via recurrent local transmission.These findings elucidate the genetic diversity and phylogenesis of MPXVs during their nascent transmission within Beijing and provide vital information to enhance future Mpox containment strategies.

Serological Investigation of Laboratory-Confirmed and Suspected Ebola Virus Disease Patients During the Late Phase of the Ebola Outbreak in Sierra Leone

This study aimed to investigate the serological characteristics of Ebola virus(EBOV) infection during the late phase of the Ebola outbreak in Sierra Leone. In total, 877 blood samples from 694 suspected Ebola virus disease(EVD) cases assessed from March to December 2015, were analyzed via real-time reverse transcription polymerase chain reaction(RT-PCR) for viral RNA and enzyme-linked immunosorbent assay(ELISA) and Luminex to detect antibodies against EBOV. Viral load and EBOV-specific IgM/IgG titers displayed a declining trend during March to December 2015. Viral RNA load decreased rapidly at earlier stages after disease onset, while EBOV-specific IgM and IgG still persisted in 58.1%(18/31) and 93.5%(29/31) of the confirmed EVD patients and in 3.8%(25/663) and 17.8%(118/663) of the RNA-negative suspected patients in the later phase, respectively. Dynamic analysis of longitudinally collected samples from eight EVD patients revealed typically reversed trends of declining viral load and increasing IgM and/or IgG titers in response to the EBOV infection.The present results indicate that certain populations of Sierra Leone developed immunity to an EBOV infection in the late phase of the outbreak, providing novel insights into the risk assessment of EBOV infections among human populations.

SMILE术后弥漫性层间角膜炎一例

患者,男,30岁,于2019年9月在山东省鲁南眼科医院行双眼飞秒激光小切口角膜基质透镜取出术(SMILE)。术前患者否认其他病史。术前检查:屈光度:右眼-4.75 D,左眼-4.50 D,矫正视力均为5.0;眼压:右眼19.7 mmHg(1 mmHg=0.133 kPa),左眼19.7 mmHg;Pentacam测量角膜厚度:右眼583μm,左眼586μm。